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The map label for this gene is purB [H]

Identifier: 45358534

GI number: 45358534

Start: 958096

End: 959445

Strand: Direct

Name: purB [H]

Synonym: MMP0971

Alternate gene names: 45358534

Gene position: 958096-959445 (Clockwise)

Preceding gene: 45358533

Following gene: 45358539

Centisome position: 57.68

GC content: 35.19

Gene sequence:

>1350_bases
ATGGCAATTCACCCTATCGACTTTAGATACGGAACTCCTGAAATGAAAAAAGTATGGGGAGAAGAAACAAAACTTCAAAA
AATGTTAGAAGTTGAAGCGGCCCTTGCTTTAGCAGAATCTGAACTTGGAATGATTCCAAAAGAAGCTGCAGAAGAAATAA
ACAGAAAAAAATCAACTGAATTTGTAAAACTTGAAAGAGTAAAAGAAATTGAAATGGCGACAAGACATGATGTTGTTTCA
GTTGTAAAAGCTTTTGCAGAACAGTGCGAAGGAACTGCTGGAGAATACATCCACTTTGGTTCCACTTCAAATGATATTAT
CGATACTGCACAGTCCCTCCAATTTAAAGATGCTTTTGAAGTTTTGGAAAGTAAATTAAAAATTTTAAGAGGGGAACTCT
TAAAAAAAGCAGAAGAGCACAAAAATACAGTTTGTATTGGAAGAACACACGGACAGCATGCAGTTCCAACCACCTACGGA
ATGAAATTTGCATTATGGGCTACAGAAATTCAAAGACATATTGAAAGACTTGAAAGCTCTAAAAAAAGGCTCTGTGTTTC
AATGATTACTGGTGCAGTTGGTACAATGGCTGCAATCGGTGAAAATGGAATTTTAGTCCACAAAAGAGTTGGAGAAATTT
TAAGCCTTGATCCAGTTTTAATTTCAAACCAGGTTGTTCAAAGAGACAGACACGGAGAATTCATGGCTACTTTAGCATTA
ATTGCACAGACTTTGAATAAAATAGGAGTTACTGTAAGAAGCATGCAGAGAAGTGAAATTAAAGAATTAGAAGAAGAATT
TGACGCGTCAAAACAGACTGGTTCATCCACAATGCCGCACAAAAGAAATCCAATTACATTTGAGCAGATTTGCGGACTTT
CAAGAATTGTAAAAGCAAATGCACTTGCAGAATTTGATAACATTCCGCTTTGGGAAGAAAGAGATTTAACAAACTCATCT
TCTGAAAGATGCTTGTTCCCAGAGTCATGCGTACTTTTGGACCACATAATAAACCTTTCGATAAAAGGAATGAGAAAATT
AAGTGTAAATCTTGAAAATGTTAATAAAAACCTTGAAATGACAAAAGGACTCATCATGGCAGAAAGAGTTATGATGTTTT
TAGCAAATAGTGGAATGGGAAGACAGACAGGTCACGAAACTGTTAGAAAATGTGCAATGAAGGCCCACGATGAAGGAAGA
CACTTAAAAGAAGTACTTTTGGAAGAGCCTGAAGTTATGAAATACGTTTCACTTGATGAAATTGAAAAAATGTTTGATTA
TAAAACATATATCGGATTAGCTCCTGAAATTGTAGAAAATGTACTCAATGAAGCTAAAAATTGGGAATAA

Upstream 100 bases:

>100_bases
GGAAGACAAATCTATAAATGACATTAATACCTTAGATGATGTGAAGAAAATATACGAAATAGAACGAAACAGAAAATAAT
TTTTTATAAAAGGTGATTAC

Downstream 100 bases:

>100_bases
CTTAAAAAAACATTTTTAATAACTATATTCTTTTTTTAAAATTAAAAAATTATTCAAAATTATAGTATACTTTTGGACCG
GTTTCCGAAGAATAAACTGA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MAIHPIDFRYGTPEMKKVWGEETKLQKMLEVEAALALAESELGMIPKEAAEEINRKKSTEFVKLERVKEIEMATRHDVVS
VVKAFAEQCEGTAGEYIHFGSTSNDIIDTAQSLQFKDAFEVLESKLKILRGELLKKAEEHKNTVCIGRTHGQHAVPTTYG
MKFALWATEIQRHIERLESSKKRLCVSMITGAVGTMAAIGENGILVHKRVGEILSLDPVLISNQVVQRDRHGEFMATLAL
IAQTLNKIGVTVRSMQRSEIKELEEEFDASKQTGSSTMPHKRNPITFEQICGLSRIVKANALAEFDNIPLWEERDLTNSS
SERCLFPESCVLLDHIINLSIKGMRKLSVNLENVNKNLEMTKGLIMAERVMMFLANSGMGRQTGHETVRKCAMKAHDEGR
HLKEVLLEEPEVMKYVSLDEIEKMFDYKTYIGLAPEIVENVLNEAKNWE

Sequences:

>Translated_449_residues
MAIHPIDFRYGTPEMKKVWGEETKLQKMLEVEAALALAESELGMIPKEAAEEINRKKSTEFVKLERVKEIEMATRHDVVS
VVKAFAEQCEGTAGEYIHFGSTSNDIIDTAQSLQFKDAFEVLESKLKILRGELLKKAEEHKNTVCIGRTHGQHAVPTTYG
MKFALWATEIQRHIERLESSKKRLCVSMITGAVGTMAAIGENGILVHKRVGEILSLDPVLISNQVVQRDRHGEFMATLAL
IAQTLNKIGVTVRSMQRSEIKELEEEFDASKQTGSSTMPHKRNPITFEQICGLSRIVKANALAEFDNIPLWEERDLTNSS
SERCLFPESCVLLDHIINLSIKGMRKLSVNLENVNKNLEMTKGLIMAERVMMFLANSGMGRQTGHETVRKCAMKAHDEGR
HLKEVLLEEPEVMKYVSLDEIEKMFDYKTYIGLAPEIVENVLNEAKNWE
>Mature_448_residues
AIHPIDFRYGTPEMKKVWGEETKLQKMLEVEAALALAESELGMIPKEAAEEINRKKSTEFVKLERVKEIEMATRHDVVSV
VKAFAEQCEGTAGEYIHFGSTSNDIIDTAQSLQFKDAFEVLESKLKILRGELLKKAEEHKNTVCIGRTHGQHAVPTTYGM
KFALWATEIQRHIERLESSKKRLCVSMITGAVGTMAAIGENGILVHKRVGEILSLDPVLISNQVVQRDRHGEFMATLALI
AQTLNKIGVTVRSMQRSEIKELEEEFDASKQTGSSTMPHKRNPITFEQICGLSRIVKANALAEFDNIPLWEERDLTNSSS
ERCLFPESCVLLDHIINLSIKGMRKLSVNLENVNKNLEMTKGLIMAERVMMFLANSGMGRQTGHETVRKCAMKAHDEGRH
LKEVLLEEPEVMKYVSLDEIEKMFDYKTYIGLAPEIVENVLNEAKNWE

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=456, Percent_Identity=27.4122807017544, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI183227688, Length=388, Percent_Identity=27.8350515463918, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI19743875, Length=370, Percent_Identity=24.0540540540541, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787376, Length=407, Percent_Identity=27.7641277641278, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1790398, Length=370, Percent_Identity=21.6216216216216, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17508577, Length=377, Percent_Identity=29.1777188328912, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI32564234, Length=310, Percent_Identity=29.3548387096774, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17553882, Length=328, Percent_Identity=28.9634146341463, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI32565146, Length=321, Percent_Identity=29.595015576324, Blast_Score=82, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6323391, Length=459, Percent_Identity=26.5795206971678, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24647570, Length=458, Percent_Identity=27.0742358078603, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24640179, Length=330, Percent_Identity=27.8787878787879, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24640177, Length=330, Percent_Identity=27.8787878787879, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24662684, Length=323, Percent_Identity=25.3869969040248, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI78710009, Length=218, Percent_Identity=29.8165137614679, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 50715; Mature: 50584

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIHPIDFRYGTPEMKKVWGEETKLQKMLEVEAALALAESELGMIPKEAAEEINRKKSTE
CCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FVKLERVKEIEMATRHDVVSVVKAFAEQCEGTAGEYIHFGSTSNDIIDTAQSLQFKDAFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHH
VLESKLKILRGELLKKAEEHKNTVCIGRTHGQHAVPTTYGMKFALWATEIQRHIERLESS
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHH
KKRLCVSMITGAVGTMAAIGENGILVHKRVGEILSLDPVLISNQVVQRDRHGEFMATLAL
HHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHH
IAQTLNKIGVTVRSMQRSEIKELEEEFDASKQTGSSTMPHKRNPITFEQICGLSRIVKAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
ALAEFDNIPLWEERDLTNSSSERCLFPESCVLLDHIINLSIKGMRKLSVNLENVNKNLEM
HHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHCCHHHHCCCHHHHCCHHHH
TKGLIMAERVMMFLANSGMGRQTGHETVRKCAMKAHDEGRHLKEVLLEEPEVMKYVSLDE
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHCCHHH
IEKMFDYKTYIGLAPEIVENVLNEAKNWE
HHHHHHHHHHHCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AIHPIDFRYGTPEMKKVWGEETKLQKMLEVEAALALAESELGMIPKEAAEEINRKKSTE
CCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FVKLERVKEIEMATRHDVVSVVKAFAEQCEGTAGEYIHFGSTSNDIIDTAQSLQFKDAFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHH
VLESKLKILRGELLKKAEEHKNTVCIGRTHGQHAVPTTYGMKFALWATEIQRHIERLESS
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHH
KKRLCVSMITGAVGTMAAIGENGILVHKRVGEILSLDPVLISNQVVQRDRHGEFMATLAL
HHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHH
IAQTLNKIGVTVRSMQRSEIKELEEEFDASKQTGSSTMPHKRNPITFEQICGLSRIVKAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
ALAEFDNIPLWEERDLTNSSSERCLFPESCVLLDHIINLSIKGMRKLSVNLENVNKNLEM
HHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHCCHHHHCCCHHHHCCHHHH
TKGLIMAERVMMFLANSGMGRQTGHETVRKCAMKAHDEGRHLKEVLLEEPEVMKYVSLDE
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHCCHHH
IEKMFDYKTYIGLAPEIVENVLNEAKNWE
HHHHHHHHHHHCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]