Click here to switch to the map view.

The map label for this gene is vhcA [H]

Identifier: 45358386

GI number: 45358386

Start: 816716

End: 818131

Strand: Direct

Name: vhcA [H]

Synonym: MMP0823

Alternate gene names: 45358386

Gene position: 816716-818131 (Clockwise)

Preceding gene: 45358385

Following gene: 45358387

Centisome position: 49.17

GC content: 38.35

Gene sequence:

>1416_bases
ATGACAAAACTGTCGATAGAACCTGTTACCAGAGTTGAAGGACACGGTAAGGTTACACTTTCCTTTGACGACAGTGGAAA
GCTCGATAAAGTTAATTTCCACGTTGTTGAAGTTAGAGGTTTTGAAAAATTCTTGGAAGGAAGATATATCGAAGATGCTC
CGGTATTTACACCAAGAATTTGTGGAATTTGCCAAGTTTCACACAATTTGGCAAGCGCAAGAGCAGTTGATAACGTATTT
GGAGTAAAAATTCCAAAAACTGCCGACATGCTCAGAAATTTAATGCAACAAGCTTCAAATGTTCACAGCCACGCGCTCCA
CTTTGGAATGCTAGCATCTCCTGATTTAATGTTCCCGATGACTGATGATGCACTTAAAAGGAACGTTTTAGGTGTAGCTT
CTGAAAACATGGACGTTGTAAAAGATGCAATTTCAATGAGAAAAATTGGTCAGACAATTATTCAAAAAGTTGGTGGAAGG
GCCATTCACCCCGTAACGGCAGTTGTTGGGGGACAGTCAAAACCATTAACGGAAGAAGAAAGGGACGAACTTTTAAAAAT
GTCAGAAAATCTTGTAGATACTGCTGAAAGAACTTTAGTAGTTGGAAAACAACTTTTGGAGGGATTAAAAGAACAAGATC
TCCTCGAACTTGGCTACTTTGAATCATACCACATGGGTATGGTAAATAACGGTGCACAAGATATTTATGAAGGAAAAATC
AGGGTTGTAAATCCAGAAGGAAAAATTGAATATGAATACGAACCTTCAGAATACTTAAACTACATGTCAGAAGGAGTAAG
GCCATATTCATACCTTAAATTCCCATACTTAACTGAAAAAGGTCCTGTTGATGGAGTTTACAGAGTAAATACTCTTTCAA
GGTTAAATGTGTGCGACAGAATGCCAACACCTATTGCTCAAAAATACTATGAAGAATTTGTAAAAACTTTTGGAAAACCT
GCACACCACCCGATGCTGTTCCACTATGCAAGATTAATCGAGCTTATTTCAAGTGCAGAACTCATAAGAAAATTCTTAGA
AGACGATGCAATTGTTGGAACTGACATAAGGGTAGAACCAACCGATCTTGTTGGAGAAGGTGTTGGATGTCTCGAAGCTC
CAAGGGGAACTTTAGTGCACCACTTCAAAACCGATGATAAGGGAATTATCACCGATGCAAATTTAGTTGTTGCAACTGTT
CAAAATAACCCTGCAATGGACATAGGCGTTCAAAAGGTTGCTGAAAAATACATAAAAACACCCGAAGATGCAAAACCGCA
TGTTTTAAACCATATGGAAATGGTAATTCGGGCATACGATCCATGCCTTTCATGTGCAACGCACACAATTGACGGAAAAG
AAAGAATGCTTTCAATGGATGTCTACAAGGGCGGAAAATTAATTAAAACATTATAG

Upstream 100 bases:

>100_bases
ACAGATACTGGATAAAGTGGGTAGTTTTTACAAATTTACGTTCCCAAGTGCATTAATTCCGATAAATAACAAAAGGTAAG
ATACTAAATACGGTGATATA

Downstream 100 bases:

>100_bases
GGGATATCATGATCAAGATAAACACTGAAGACTGTTATATGTGTAAAGCTTGTGAAAATAGCTGCCCTACAGAAGCTTTA
AAATTAAATCCCTTTAAAGT

Product: coenzyme F420-non-reducing hydrogenase subunit alpha

Products: 2 Oxidized Ferredoxin; H(2). [C]

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MTKLSIEPVTRVEGHGKVTLSFDDSGKLDKVNFHVVEVRGFEKFLEGRYIEDAPVFTPRICGICQVSHNLASARAVDNVF
GVKIPKTADMLRNLMQQASNVHSHALHFGMLASPDLMFPMTDDALKRNVLGVASENMDVVKDAISMRKIGQTIIQKVGGR
AIHPVTAVVGGQSKPLTEEERDELLKMSENLVDTAERTLVVGKQLLEGLKEQDLLELGYFESYHMGMVNNGAQDIYEGKI
RVVNPEGKIEYEYEPSEYLNYMSEGVRPYSYLKFPYLTEKGPVDGVYRVNTLSRLNVCDRMPTPIAQKYYEEFVKTFGKP
AHHPMLFHYARLIELISSAELIRKFLEDDAIVGTDIRVEPTDLVGEGVGCLEAPRGTLVHHFKTDDKGIITDANLVVATV
QNNPAMDIGVQKVAEKYIKTPEDAKPHVLNHMEMVIRAYDPCLSCATHTIDGKERMLSMDVYKGGKLIKTL

Sequences:

>Translated_471_residues
MTKLSIEPVTRVEGHGKVTLSFDDSGKLDKVNFHVVEVRGFEKFLEGRYIEDAPVFTPRICGICQVSHNLASARAVDNVF
GVKIPKTADMLRNLMQQASNVHSHALHFGMLASPDLMFPMTDDALKRNVLGVASENMDVVKDAISMRKIGQTIIQKVGGR
AIHPVTAVVGGQSKPLTEEERDELLKMSENLVDTAERTLVVGKQLLEGLKEQDLLELGYFESYHMGMVNNGAQDIYEGKI
RVVNPEGKIEYEYEPSEYLNYMSEGVRPYSYLKFPYLTEKGPVDGVYRVNTLSRLNVCDRMPTPIAQKYYEEFVKTFGKP
AHHPMLFHYARLIELISSAELIRKFLEDDAIVGTDIRVEPTDLVGEGVGCLEAPRGTLVHHFKTDDKGIITDANLVVATV
QNNPAMDIGVQKVAEKYIKTPEDAKPHVLNHMEMVIRAYDPCLSCATHTIDGKERMLSMDVYKGGKLIKTL
>Mature_470_residues
TKLSIEPVTRVEGHGKVTLSFDDSGKLDKVNFHVVEVRGFEKFLEGRYIEDAPVFTPRICGICQVSHNLASARAVDNVFG
VKIPKTADMLRNLMQQASNVHSHALHFGMLASPDLMFPMTDDALKRNVLGVASENMDVVKDAISMRKIGQTIIQKVGGRA
IHPVTAVVGGQSKPLTEEERDELLKMSENLVDTAERTLVVGKQLLEGLKEQDLLELGYFESYHMGMVNNGAQDIYEGKIR
VVNPEGKIEYEYEPSEYLNYMSEGVRPYSYLKFPYLTEKGPVDGVYRVNTLSRLNVCDRMPTPIAQKYYEEFVKTFGKPA
HHPMLFHYARLIELISSAELIRKFLEDDAIVGTDIRVEPTDLVGEGVGCLEAPRGTLVHHFKTDDKGIITDANLVVATVQ
NNPAMDIGVQKVAEKYIKTPEDAKPHVLNHMEMVIRAYDPCLSCATHTIDGKERMLSMDVYKGGKLIKTL

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=124, Percent_Identity=36.2903225806452, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789368, Length=115, Percent_Identity=32.1739130434783, Blast_Score=82, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: 1.12.99.6 [C]

Molecular weight: Translated: 52673; Mature: 52542

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLSIEPVTRVEGHGKVTLSFDDSGKLDKVNFHVVEVRGFEKFLEGRYIEDAPVFTPRI
CCCCCCCCCCEECCCCEEEEEECCCCCEEEEEEEEEEECCHHHHHCCCCCCCCCCCCHHH
CGICQVSHNLASARAVDNVFGVKIPKTADMLRNLMQQASNVHSHALHFGMLASPDLMFPM
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCC
TDDALKRNVLGVASENMDVVKDAISMRKIGQTIIQKVGGRAIHPVTAVVGGQSKPLTEEE
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHEECCCCCCCCHHH
RDELLKMSENLVDTAERTLVVGKQLLEGLKEQDLLELGYFESYHMGMVNNGAQDIYEGKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHCCCCCCCCHHHHHCCCE
RVVNPEGKIEYEYEPSEYLNYMSEGVRPYSYLKFPYLTEKGPVDGVYRVNTLSRLNVCDR
EEECCCCCEEEEECHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCEEEECHHHHHHHHHC
MPTPIAQKYYEEFVKTFGKPAHHPMLFHYARLIELISSAELIRKFLEDDAIVGTDIRVEP
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEECC
TDLVGEGVGCLEAPRGTLVHHFKTDDKGIITDANLVVATVQNNPAMDIGVQKVAEKYIKT
HHHHCCCCCCCCCCCCCEEEEEECCCCCEEEECCEEEEEECCCCCHHHHHHHHHHHHHCC
PEDAKPHVLNHMEMVIRAYDPCLSCATHTIDGKERMLSMDVYKGGKLIKTL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEHHHHCCCEEECCC
>Mature Secondary Structure 
TKLSIEPVTRVEGHGKVTLSFDDSGKLDKVNFHVVEVRGFEKFLEGRYIEDAPVFTPRI
CCCCCCCCCEECCCCEEEEEECCCCCEEEEEEEEEEECCHHHHHCCCCCCCCCCCCHHH
CGICQVSHNLASARAVDNVFGVKIPKTADMLRNLMQQASNVHSHALHFGMLASPDLMFPM
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCC
TDDALKRNVLGVASENMDVVKDAISMRKIGQTIIQKVGGRAIHPVTAVVGGQSKPLTEEE
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHEECCCCCCCCHHH
RDELLKMSENLVDTAERTLVVGKQLLEGLKEQDLLELGYFESYHMGMVNNGAQDIYEGKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHCCCCCCCCHHHHHCCCE
RVVNPEGKIEYEYEPSEYLNYMSEGVRPYSYLKFPYLTEKGPVDGVYRVNTLSRLNVCDR
EEECCCCCEEEEECHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCEEEECHHHHHHHHHC
MPTPIAQKYYEEFVKTFGKPAHHPMLFHYARLIELISSAELIRKFLEDDAIVGTDIRVEP
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEECC
TDLVGEGVGCLEAPRGTLVHHFKTDDKGIITDANLVVATVQNNPAMDIGVQKVAEKYIKT
HHHHCCCCCCCCCCCCCEEEEEECCCCCEEEECCEEEEEECCCCCHHHHHHHHHHHHHCC
PEDAKPHVLNHMEMVIRAYDPCLSCATHTIDGKERMLSMDVYKGGKLIKTL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Iron-Sulfur Cluster. [C]

Metal ions: Ni; Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 Reduced Ferredoxin; 2 H(+) [C]

Specific reaction: 2 Reduced Ferredoxin + 2 H(+) = 2 Oxidized Ferredoxin + H(2). [C]

General reaction: Redox reaction [C]

Inhibitor: Dimethyl sulfoxide; EDTA; NaCl; Tiron; Urea [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1603063 [H]