Click here to switch to the map view.

The map label for this gene is dph5 [H]

Identifier: 45358151

GI number: 45358151

Start: 584564

End: 585331

Strand: Direct

Name: dph5 [H]

Synonym: MMP0588

Alternate gene names: 45358151

Gene position: 584564-585331 (Clockwise)

Preceding gene: 45358148

Following gene: 45358152

Centisome position: 35.19

GC content: 33.46

Gene sequence:

>768_bases
ATGCTTGTAATGGCAGGACTTGGACTTTACGATGAAAGAGACGTTACACTAAAAACGCTTGACTTTGCAAAAAAAGTTGA
TAAAATTTACGCTGAATTTTACACGGCAATACTTACTGGAACAACGATGGAAAAAATTGAAGGTACGCTTCAAAAACCCA
TAACTGTTTTGGATCGAGAAAAAGTAGAGTATGAAACGAATAAATTAATTGACGAAGCAAAAGATAAGGATATAATGTTT
TTAACTGCAGGAGACCCGATGGTTGCAACAACACACGTGGATATAGCAGTTGAAGCTAGAAAAAAAGGAATCGAAGTTGT
AATTATAAATGCTCCTTCAATTTACTCGGCAATAGGAATTACGGGACTTCAATTATACAAATTTGGAAAAACAACGTCAA
TAGTATTTCCCGAACCAAATTATTTCCCGGAAACCCCTTATGACGTAATAAAAGATAATTTAAAATTAGGATACCACACA
CTCTGCCTTTTAGATATTCAGGCAGATAAGGAAAGATTCATGACTGCAAACGAAGGATTGGATGCACTTTTAAAAATTGA
GGAAAAAAGAAAAGAAAACGTAATTTCTGAGGAAACAGAAGTTGCGGTTGTTGCAAGAGCTGGAAGTATTAATCCTGGAT
TATACTATGGAAAAATAAAAGATCTTTTAAATTATGATTTTAAAAGCCCGCTTCACTGTGTGATAATTCCTGGAAAACTT
CACTTTATGGAAGAAGACGCTTTAAAATACTTATTTGAAAATATTTAA

Upstream 100 bases:

>100_bases
CAGTTAGAATTTACGTTATGATTTTTAATTTTACAATTAAGTTTATGAGCTATATAGTATTATTGATATTATTTCTATTT
ATTAAATCGGGTGAAACACA

Downstream 100 bases:

>100_bases
AAGTTTTAAGCTTCTTCGCACTGTGTTGTAGGCATTGACGACTTTGCAAGTAAATAAGTTGCAGTAATTAGTGCAAAGAC
GTAAAAAACGTGCATAATGT

Product: diphthine synthase

Products: NA

Alternate protein names: Diphthamide biosynthesis methyltransferase [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MLVMAGLGLYDERDVTLKTLDFAKKVDKIYAEFYTAILTGTTMEKIEGTLQKPITVLDREKVEYETNKLIDEAKDKDIMF
LTAGDPMVATTHVDIAVEARKKGIEVVIINAPSIYSAIGITGLQLYKFGKTTSIVFPEPNYFPETPYDVIKDNLKLGYHT
LCLLDIQADKERFMTANEGLDALLKIEEKRKENVISEETEVAVVARAGSINPGLYYGKIKDLLNYDFKSPLHCVIIPGKL
HFMEEDALKYLFENI

Sequences:

>Translated_255_residues
MLVMAGLGLYDERDVTLKTLDFAKKVDKIYAEFYTAILTGTTMEKIEGTLQKPITVLDREKVEYETNKLIDEAKDKDIMF
LTAGDPMVATTHVDIAVEARKKGIEVVIINAPSIYSAIGITGLQLYKFGKTTSIVFPEPNYFPETPYDVIKDNLKLGYHT
LCLLDIQADKERFMTANEGLDALLKIEEKRKENVISEETEVAVVARAGSINPGLYYGKIKDLLNYDFKSPLHCVIIPGKL
HFMEEDALKYLFENI
>Mature_255_residues
MLVMAGLGLYDERDVTLKTLDFAKKVDKIYAEFYTAILTGTTMEKIEGTLQKPITVLDREKVEYETNKLIDEAKDKDIMF
LTAGDPMVATTHVDIAVEARKKGIEVVIINAPSIYSAIGITGLQLYKFGKTTSIVFPEPNYFPETPYDVIKDNLKLGYHT
LCLLDIQADKERFMTANEGLDALLKIEEKRKENVISEETEVAVVARAGSINPGLYYGKIKDLLNYDFKSPLHCVIIPGKL
HFMEEDALKYLFENI

Specific function: Required for the methylation step in diphthamide biosynthesis [H]

COG id: COG1798

COG function: function code J; Diphthamide biosynthesis methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diphthine synthase family [H]

Homologues:

Organism=Homo sapiens, GI117190324, Length=268, Percent_Identity=37.3134328358209, Blast_Score=178, Evalue=4e-45,
Organism=Homo sapiens, GI117190326, Length=269, Percent_Identity=36.8029739776952, Blast_Score=178, Evalue=5e-45,
Organism=Homo sapiens, GI117190262, Length=269, Percent_Identity=36.8029739776952, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17531369, Length=269, Percent_Identity=37.546468401487, Blast_Score=168, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6323201, Length=254, Percent_Identity=37.7952755905512, Blast_Score=184, Evalue=1e-47,
Organism=Drosophila melanogaster, GI28572120, Length=269, Percent_Identity=35.6877323420074, Blast_Score=164, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR004551 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.98 [H]

Molecular weight: Translated: 28772; Mature: 28772

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVMAGLGLYDERDVTLKTLDFAKKVDKIYAEFYTAILTGTTMEKIEGTLQKPITVLDRE
CEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHCHH
KVEYETNKLIDEAKDKDIMFLTAGDPMVATTHVDIAVEARKKGIEVVIINAPSIYSAIGI
HHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEEEEEEEECCCCCEEEEEECCCHHHHHCC
TGLQLYKFGKTTSIVFPEPNYFPETPYDVIKDNLKLGYHTLCLLDIQADKERFMTANEGL
CCEEEEECCCEEEEEECCCCCCCCCCHHHHHCCCCCCEEEEEEEEEECCHHHHEECCCCH
DALLKIEEKRKENVISEETEVAVVARAGSINPGLYYGKIKDLLNYDFKSPLHCVIIPGKL
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEHHHHHHHHCCCCCCCCEEEEECCEE
HFMEEDALKYLFENI
EECHHHHHHHHHHCC
>Mature Secondary Structure
MLVMAGLGLYDERDVTLKTLDFAKKVDKIYAEFYTAILTGTTMEKIEGTLQKPITVLDRE
CEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHCHH
KVEYETNKLIDEAKDKDIMFLTAGDPMVATTHVDIAVEARKKGIEVVIINAPSIYSAIGI
HHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEEEEEEEECCCCCEEEEEECCCHHHHHCC
TGLQLYKFGKTTSIVFPEPNYFPETPYDVIKDNLKLGYHTLCLLDIQADKERFMTANEGL
CCEEEEECCCEEEEEECCCCCCCCCCHHHHHCCCCCCEEEEEEEEEECCHHHHEECCCCH
DALLKIEEKRKENVISEETEVAVVARAGSINPGLYYGKIKDLLNYDFKSPLHCVIIPGKL
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEHHHHHHHHCCCCCCCCEEEEECCEE
HFMEEDALKYLFENI
EECHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA