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The map label for this gene is metE [H]

Identifier: 45357964

GI number: 45357964

Start: 399758

End: 400696

Strand: Direct

Name: metE [H]

Synonym: MMP0401

Alternate gene names: 45357964

Gene position: 399758-400696 (Clockwise)

Preceding gene: 45357963

Following gene: 45357965

Centisome position: 24.07

GC content: 31.31

Gene sequence:

>939_bases
ATGATAAAAACTGTTGTTGGTAGCTACCCAGTAGTTAAAGGAAAACCTGATTCTATACCGGATAAAATTAAGAAATTTTT
TGGAAAATATGATAAATACGAATATTCGATAAAAAAAGCGTTAGATGATCAATTAAACGTCGGAATTGATATATTAAGTG
ACGGCCAGGTCAGAGGAGACATGGTTGAGATATTTGTATCAAATATGTATGGTTTTGAAGGCAGGAGAGTTGTCAACAGG
GTTGAATTTGTAAAACCAATCACTTTAAACGATATAAAATATTCTTTAAAATACATTTCAAAAAAAGACCCTGAAAAAGG
AGTAAAAGGAATAATTACTGGAGCATGCACGTTAGCTTCATCCATTCGTGTTGAAAATTATTATTCCGATAACAAAGATG
AAAATTTAATTTACGATTTAGCAGGGGCTTTAAACAAAGAAGCTCTTTCGATTCAAAATCATGTAAAAATGATACAGTTT
GATGAGCCAATCCTTTCAACTGGGTTATACGATCTTGAAGTTGCAAAAAAAGCAATGGATATTATTACTTCTGGAATAAA
TGTTCCTGTTGCAATGCACGTCTGCGGAGATGTTTCAAAAATATTTTACAAATTAAATGAATTCAATGTCGATATTTTGG
ACCACGAATTTGCATCATGCAGAAAAAATCTTGAAATATTAAATGAAATCACAAAAAAGGTTGGTTTTGGGTGTATAAAC
ACTAAACTTAAATCGGTAGATTCTGTCGACGAAGTAAAAGCATTAATAAGTGAAGGAATAGAAATATTAAAGAATAATTC
TAAATTTGAAGGTTCAATAAATGATTCCGTTATAATCGATCCCGACTGCGGTATGAGACTGCTTCCAGTGGATGTTGCAT
ACTCTAAATTAAATAACATGGTTACTGCAGCAAATGAGATTGAAAAGGATTTAATTTAA

Upstream 100 bases:

>100_bases
CGGTAATGACTGTTGCAGATACTGCTAAATTTGACATAACTAAGGTTCGCGGAAGAATACTTTAATTTTATATTATTTTT
TTTATTTTTTTGGTGATTTT

Downstream 100 bases:

>100_bases
ACTTTTAGGTAAGATACAATGTCAAAGCCAATTTTAAATAAGTATAAGCTGTATTTATTAGTCGCCCTAATGTTCTGTTC
TATAAGTGCAGTTGATTCGT

Product: methionine synthase

Products: tetrahydropteroyltri-L-glutamate; L-methionine

Alternate protein names: Methionine synthase [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MIKTVVGSYPVVKGKPDSIPDKIKKFFGKYDKYEYSIKKALDDQLNVGIDILSDGQVRGDMVEIFVSNMYGFEGRRVVNR
VEFVKPITLNDIKYSLKYISKKDPEKGVKGIITGACTLASSIRVENYYSDNKDENLIYDLAGALNKEALSIQNHVKMIQF
DEPILSTGLYDLEVAKKAMDIITSGINVPVAMHVCGDVSKIFYKLNEFNVDILDHEFASCRKNLEILNEITKKVGFGCIN
TKLKSVDSVDEVKALISEGIEILKNNSKFEGSINDSVIIDPDCGMRLLPVDVAYSKLNNMVTAANEIEKDLI

Sequences:

>Translated_312_residues
MIKTVVGSYPVVKGKPDSIPDKIKKFFGKYDKYEYSIKKALDDQLNVGIDILSDGQVRGDMVEIFVSNMYGFEGRRVVNR
VEFVKPITLNDIKYSLKYISKKDPEKGVKGIITGACTLASSIRVENYYSDNKDENLIYDLAGALNKEALSIQNHVKMIQF
DEPILSTGLYDLEVAKKAMDIITSGINVPVAMHVCGDVSKIFYKLNEFNVDILDHEFASCRKNLEILNEITKKVGFGCIN
TKLKSVDSVDEVKALISEGIEILKNNSKFEGSINDSVIIDPDCGMRLLPVDVAYSKLNNMVTAANEIEKDLI
>Mature_312_residues
MIKTVVGSYPVVKGKPDSIPDKIKKFFGKYDKYEYSIKKALDDQLNVGIDILSDGQVRGDMVEIFVSNMYGFEGRRVVNR
VEFVKPITLNDIKYSLKYISKKDPEKGVKGIITGACTLASSIRVENYYSDNKDENLIYDLAGALNKEALSIQNHVKMIQF
DEPILSTGLYDLEVAKKAMDIITSGINVPVAMHVCGDVSKIFYKLNEFNVDILDHEFASCRKNLEILNEITKKVGFGCIN
TKLKSVDSVDEVKALISEGIEILKNNSKFEGSINDSVIIDPDCGMRLLPVDVAYSKLNNMVTAANEIEKDLI

Specific function: Catalyzes the transfer of a methyl group from methylcobalamin and methylcobinamide to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022921
- InterPro:   IPR002629 [H]

Pfam domain/function: PF01717 Meth_synt_2 [H]

EC number: 2.1.1.14

Molecular weight: Translated: 34831; Mature: 34831

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKTVVGSYPVVKGKPDSIPDKIKKFFGKYDKYEYSIKKALDDQLNVGIDILSDGQVRGD
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHH
MVEIFVSNMYGFEGRRVVNRVEFVKPITLNDIKYSLKYISKKDPEKGVKGIITGACTLAS
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
SIRVENYYSDNKDENLIYDLAGALNKEALSIQNHVKMIQFDEPILSTGLYDLEVAKKAMD
HHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHEEEEECCHHHHCCCHHHHHHHHHHH
IITSGINVPVAMHVCGDVSKIFYKLNEFNVDILDHEFASCRKNLEILNEITKKVGFGCIN
HHHCCCCCCEEEHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHH
TKLKSVDSVDEVKALISEGIEILKNNSKFEGSINDSVIIDPDCGMRLLPVDVAYSKLNNM
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCEEEEHHHHHHHHHHH
VTAANEIEKDLI
HHHHHHHHHCCC
>Mature Secondary Structure
MIKTVVGSYPVVKGKPDSIPDKIKKFFGKYDKYEYSIKKALDDQLNVGIDILSDGQVRGD
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHH
MVEIFVSNMYGFEGRRVVNRVEFVKPITLNDIKYSLKYISKKDPEKGVKGIITGACTLAS
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
SIRVENYYSDNKDENLIYDLAGALNKEALSIQNHVKMIQFDEPILSTGLYDLEVAKKAMD
HHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHEEEEECCHHHHCCCHHHHHHHHHHH
IITSGINVPVAMHVCGDVSKIFYKLNEFNVDILDHEFASCRKNLEILNEITKKVGFGCIN
HHHCCCCCCEEEHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHH
TKLKSVDSVDEVKALISEGIEILKNNSKFEGSINDSVIIDPDCGMRLLPVDVAYSKLNNM
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCEEEEHHHHHHHHHHH
VTAANEIEKDLI
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5-methyltetrahydropteroyltri-L-glutamate; L-homocysteine

Specific reaction: 5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]