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The map label for this gene is cofF

Identifier: 45357733

GI number: 45357733

Start: 178521

End: 179378

Strand: Direct

Name: cofF

Synonym: MMP0170

Alternate gene names: 45357733

Gene position: 178521-179378 (Clockwise)

Preceding gene: 45357732

Following gene: 45357734

Centisome position: 10.75

GC content: 33.45

Gene sequence:

>858_bases
ATGATAACAATTGCTTGTGCAGAAGGGGGGTCAACAATATACTCCCTAAAAAAAGCTATTGAAGATTTGGGCGAAAAATG
TAATATTTTATTACTTTCTAGCGATAATTTGTTAGTTGATACGGATTTCAATATAAAAACTGATTTAATACACTCAAGAT
GCGGTATCGGGGATTATTTAGATAGGCTGACCCTTTTTTCATGGCAGGTTTTAAAAAATCTGGAATCTGAAGGCCACTAT
TTTATAAATCCACTTGAAACAATCTATAATTCATCGGATAAATTTAAAACAACGAAAATTCTTAGTAAAAACGGGTTAAA
AACGCCAAAAACTGCACTCATTAGGGATTACGCGGATGCAAAACATTTTTTAGATACTAAAAATATGAACTACCCGGTTA
TATTGAAAAATTCATTTTCAAAATGCGGAATGAAAGTACAAAAAGCGAATTCTGACGATGAATTAAAAAAACTTTCCAAA
AATTCGATATGGGAAAGCAAACTAATTCAGGAATACGTTGATTTTAAAAATGGGGACACTTATAAGGATATGAGAATACT
CGTAATTGATGGGGAAGTTGTTGGTGGGTATCGGCGAGTAAGTAATAACTTCATAACGAACCTATATGTTGGCGGTCAAA
TCGAGCCTTTAAATGTATCCTCAGAACTTGAAGAAATCGCTTTAAAGTGTTCTGAGTGCATGAATGGATATATTATGGGA
ATTGACATCTTACCAAAAGATGGAGAGTACTACGTTGTTGAGGTAAATACTGCACCGGGTACAAAAGGCTTTAGGTCCCT
TGGAATTGATGTTGATAAAAGAATTGCAGAATGCCTTATAAAATACAAAAAAAGCTAA

Upstream 100 bases:

>100_bases
TAGTGAAAGGAATTTAAACTCGATAAGTATATTTGTATGGCTGGCAATACTAGCGTCAATGATTTATTCATTTATTTAAA
CGTTAATTTTCAGGTGGATG

Downstream 100 bases:

>100_bases
AAGATGATAAAAGTGTTTGAATCTCTTTTAATTTCTTTTTCATTCGTTTTACTTTCTTTAATATTAATTTTTAAGGAAAA
ACTAGGGATCGGAAAAGAAA

Product: RimK family alpha-L-glutamate ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MITIACAEGGSTIYSLKKAIEDLGEKCNILLLSSDNLLVDTDFNIKTDLIHSRCGIGDYLDRLTLFSWQVLKNLESEGHY
FINPLETIYNSSDKFKTTKILSKNGLKTPKTALIRDYADAKHFLDTKNMNYPVILKNSFSKCGMKVQKANSDDELKKLSK
NSIWESKLIQEYVDFKNGDTYKDMRILVIDGEVVGGYRRVSNNFITNLYVGGQIEPLNVSSELEEIALKCSECMNGYIMG
IDILPKDGEYYVVEVNTAPGTKGFRSLGIDVDKRIAECLIKYKKS

Sequences:

>Translated_285_residues
MITIACAEGGSTIYSLKKAIEDLGEKCNILLLSSDNLLVDTDFNIKTDLIHSRCGIGDYLDRLTLFSWQVLKNLESEGHY
FINPLETIYNSSDKFKTTKILSKNGLKTPKTALIRDYADAKHFLDTKNMNYPVILKNSFSKCGMKVQKANSDDELKKLSK
NSIWESKLIQEYVDFKNGDTYKDMRILVIDGEVVGGYRRVSNNFITNLYVGGQIEPLNVSSELEEIALKCSECMNGYIMG
IDILPKDGEYYVVEVNTAPGTKGFRSLGIDVDKRIAECLIKYKKS
>Mature_285_residues
MITIACAEGGSTIYSLKKAIEDLGEKCNILLLSSDNLLVDTDFNIKTDLIHSRCGIGDYLDRLTLFSWQVLKNLESEGHY
FINPLETIYNSSDKFKTTKILSKNGLKTPKTALIRDYADAKHFLDTKNMNYPVILKNSFSKCGMKVQKANSDDELKKLSK
NSIWESKLIQEYVDFKNGDTYKDMRILVIDGEVVGGYRRVSNNFITNLYVGGQIEPLNVSSELEEIALKCSECMNGYIMG
IDILPKDGEYYVVEVNTAPGTKGFRSLGIDVDKRIAECLIKYKKS

Specific function: Catalyzes the ATP-dependent addition of one L-glutamate molecule to gamma-F420-2, producing the mature coenzyme alpha- F420-3

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1787076, Length=214, Percent_Identity=26.6355140186916, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COFF_METMP (Q6M0U8)

Other databases:

- EMBL:   BX950229
- RefSeq:   NP_987290.1
- ProteinModelPortal:   Q6M0U8
- GeneID:   2761868
- GenomeReviews:   BX950229_GR
- KEGG:   mmp:MMP0170
- NMPDR:   fig|267377.1.peg.170
- HOGENOM:   HBG541036
- OMA:   HSRCGIG
- ProtClustDB:   CLSK876420
- BioCyc:   MMAR267377:MMP0170-MONOMER
- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- TIGRFAMs:   TIGR00768

Pfam domain/function: PF08443 RimK

EC number: NA

Molecular weight: Translated: 32127; Mature: 32127

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIACAEGGSTIYSLKKAIEDLGEKCNILLLSSDNLLVDTDFNIKTDLIHSRCGIGDYL
CEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCCCHHHHHHHHCCHHHHH
DRLTLFSWQVLKNLESEGHYFINPLETIYNSSDKFKTTKILSKNGLKTPKTALIRDYADA
HHHHHHHHHHHHHHCCCCCEEECCHHHHHCCCCCEEHHEEHHCCCCCCCHHHHHHHHHHH
KHFLDTKNMNYPVILKNSFSKCGMKVQKANSDDELKKLSKNSIWESKLIQEYVDFKNGDT
HHHHCCCCCCCCEEECCCHHHCCCEEECCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
YKDMRILVIDGEVVGGYRRVSNNFITNLYVGGQIEPLNVSSELEEIALKCSECMNGYIMG
CCCEEEEEEECCEECCHHHHCCCCEEEEEECCEEEECCCHHHHHHHHHHHHHHCCCEEEE
IDILPKDGEYYVVEVNTAPGTKGFRSLGIDVDKRIAECLIKYKKS
EEEEECCCCEEEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MITIACAEGGSTIYSLKKAIEDLGEKCNILLLSSDNLLVDTDFNIKTDLIHSRCGIGDYL
CEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCCCHHHHHHHHCCHHHHH
DRLTLFSWQVLKNLESEGHYFINPLETIYNSSDKFKTTKILSKNGLKTPKTALIRDYADA
HHHHHHHHHHHHHHCCCCCEEECCHHHHHCCCCCEEHHEEHHCCCCCCCHHHHHHHHHHH
KHFLDTKNMNYPVILKNSFSKCGMKVQKANSDDELKKLSKNSIWESKLIQEYVDFKNGDT
HHHHCCCCCCCCEEECCCHHHCCCEEECCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
YKDMRILVIDGEVVGGYRRVSNNFITNLYVGGQIEPLNVSSELEEIALKCSECMNGYIMG
CCCEEEEEEECCEECCHHHHCCCCEEEEEECCEEEECCCHHHHHHHHHHHHHHCCCEEEE
IDILPKDGEYYVVEVNTAPGTKGFRSLGIDVDKRIAECLIKYKKS
EEEEECCCCEEEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA