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The map label for this gene is ogt [C]

Identifier: 45357632

GI number: 45357632

Start: 80649

End: 80948

Strand: Direct

Name: ogt [C]

Synonym: MMP0069

Alternate gene names: 45357632

Gene position: 80649-80948 (Clockwise)

Preceding gene: 45357631

Following gene: 45357633

Centisome position: 4.86

GC content: 37.0

Gene sequence:

>300_bases
ATGAAAACATTCAATGAACAGTGCTACGACCTTATAATGCAGATTCCGAGAGGAAAAGTCACCACGTACAAAATAATAGC
CGAAGCTCTAAATACTAAAGCTTATCGGGCTGTAGGAAATGCGATGAAAAATAATCCAAAAATTTTAACAGTGCCGTGCC
ACAGGGTTGTAAATTCTAACGGTTACGTTGGCGGATATGTCAACGGGGTTGAAAAAAAGATAGAAATTTTAAAAAACGAG
GGGGTGGTTATTTCGGGGGGTAAAATAACCGATTTAAAGAAATATTTATTCGTGTTTTAG

Upstream 100 bases:

>100_bases
TTCAATCCACAAACTCCCGGCATACCCTAAAGAAAACTAAATATTAACTTTTATTTTTTTTAGAAATTTATTTTTATATT
TTAAAATCAAATTATTTTTT

Downstream 100 bases:

>100_bases
GTCTATTATCGTTGGATCTTTATTGGGCCATTTAACTGTCGACAATAATGATTTTAAATGTTTTCAGCAAATATTTTTTT
GAGGGGTTTAATATTCATTG

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: Probable methylated-DNA--protein-cysteine methyltransferase; O-6-methylbase-DNA-alkyltransferase; Endonuclease V; Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V [H]

Number of amino acids: Translated: 99; Mature: 99

Protein sequence:

>99_residues
MKTFNEQCYDLIMQIPRGKVTTYKIIAEALNTKAYRAVGNAMKNNPKILTVPCHRVVNSNGYVGGYVNGVEKKIEILKNE
GVVISGGKITDLKKYLFVF

Sequences:

>Translated_99_residues
MKTFNEQCYDLIMQIPRGKVTTYKIIAEALNTKAYRAVGNAMKNNPKILTVPCHRVVNSNGYVGGYVNGVEKKIEILKNE
GVVISGGKITDLKKYLFVF
>Mature_99_residues
MKTFNEQCYDLIMQIPRGKVTTYKIIAEALNTKAYRAVGNAMKNNPKILTVPCHRVVNSNGYVGGYVNGVEKKIEILKNE
GVVISGGKITDLKKYLFVF

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the endonuclease V family [H]

Homologues:

Organism=Escherichia coli, GI1787596, Length=82, Percent_Identity=40.2439024390244, Blast_Score=67, Evalue=3e-13,
Organism=Escherichia coli, GI1786659, Length=96, Percent_Identity=34.375, Blast_Score=66, Evalue=6e-13,
Organism=Escherichia coli, GI1788542, Length=79, Percent_Identity=36.7088607594937, Blast_Score=60, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115533070, Length=81, Percent_Identity=40.7407407407407, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=41.25, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007581
- InterPro:   IPR014048
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF04493 Endonuclease_5 [H]

EC number: =3.1.21.7 [H]

Molecular weight: Translated: 11070; Mature: 11070

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTFNEQCYDLIMQIPRGKVTTYKIIAEALNTKAYRAVGNAMKNNPKILTVPCHRVVNSN
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHHHHHHHHCCCCEEEEECCHHHHCCC
GYVGGYVNGVEKKIEILKNEGVVISGGKITDLKKYLFVF
CCCCCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHCC
>Mature Secondary Structure
MKTFNEQCYDLIMQIPRGKVTTYKIIAEALNTKAYRAVGNAMKNNPKILTVPCHRVVNSN
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHHHHHHHHCCCCEEEEECCHHHHCCC
GYVGGYVNGVEKKIEILKNEGVVISGGKITDLKKYLFVF
CCCCCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA