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The map label for this gene is mpl [H]

Identifier: 42523429

GI number: 42523429

Start: 1864805

End: 1866160

Strand: Direct

Name: mpl [H]

Synonym: Bd1952

Alternate gene names: 42523429

Gene position: 1864805-1866160 (Clockwise)

Preceding gene: 42523428

Following gene: 42523431

Centisome position: 49.29

GC content: 49.48

Gene sequence:

>1356_bases
ATGGCCTCCATGGCAGGTCTTTTGAAAGACCGTGGCTTTAAAATCACCGGCAGTGATTCCAATCCGTATCCTCCGATGTC
AACCCAGCTGGAAAGTCTGGGCATCAGTATCATGAAAGGCTACAAAGCCGAAAATCTGCATCCGAAGCCGGACTTCGTGA
TCGTGGGGAACGTGATTTCTGCGAGCAACGAGGAAGCTCAGGAGCTGATGAAGCTGGGCATCCCGTATACTTCTTTGCCA
AAAGCGATGGGTGAATTCATCATCGAAAATCGTGAAAGCGTTGTGATCTCCGGTACACACGGGAAAACCACAACAACTTC
CATGATGGCGTGGGTGGCAGAAAATGCCGGAGTAAAACCGGGTTTCCTGATCGGGGGGATTCCGAAGAACTTCTCTCAAA
GTTTCAAAAATCCGGAAGACAATTACTTCATCATCGAAGGTGATGAATACGACACGGCCTTCTTCGATAAAGTTCCAAAG
TTCATTCACTATCGTCCAAAACACGTGATCCTGACTTCGGTGGAGTTTGATCACGCGGATATCTATAAAGACCTGGATGA
CGTGAAAAAATCCTTCGCTCGTTTGATGGATCTTCTTCCTGAAAATGGAACGTTGCTGGCTTGCGCGGAAGACGCCAATG
TGATGGAGCTTCGCAAGCTTACCAAGTGCAAAAACTCCTTCACTTACGGCTTTGGCGCTGAGGCGGATTTCCGTGCGAAG
ATCATGTTCCAAAACGCCAATGGTCTGGGCTTTGAAGTTCATCACCGTGGCGAGATCATGGGCCCTTATGCTATGCAGAT
CACGGGTGAATACAACGTCCTGAATGCCACAGCTGTTGTGGCGATGTCCAAGATGCTGGGCTTCTCTGAAAACCGCATTC
AGATCGCATTGGAATCCTTCGAAGGTGTGAAGCGCCGTCAGGAAATTCTGGGTGAACCCAACGGTATTTTGGTGATTGAA
GATTTCGCCCACCACCCAACAGCGGTGCGTGAAACAGTGAAAGGCATTCAAAAGAAATACCCGGGCCGCAAAGTGTTCTC
GGTGTTTGAGCCAAGAAGTGCGACGTCCCGCCGTAAAGTCTTCCAGAAAGACTATGTGGAGGCGTTCAAGGGCTCTCACG
AAGTTCTTCTGGCAAAAGCTTTTGATCAGACCAAAATCGACGAAGAAAACCGTTTCTCCACGCATGAGCTGGTCGATGAC
CTCAAGAAAACCCATGTGACCGCAGCGGACTTCGATACAGCTGACGACATCGTCGCAGCCTTGAAATCCAAAGCCCACAA
AGGCGACGTCATCCTAATCATGAGCAACGGCGGCTTCGACGGCATCTACACCAAACTCCTAAAAGCCCTCGAATAA

Upstream 100 bases:

>100_bases
ATTCATAATTGGATTGTTGAAAACTACAGAAGAAGTGGACTTTAAGATGAATATCAAACCAGGCAGTCACATTCACTTGA
TGGGTATTTGCGGGACGGCG

Downstream 100 bases:

>100_bases
AAAAGGTACCTGCTTACTTTTGCATAAACGCCGCGTCGGTCTTCTCTCCTGATCTTAGTAAGGTAGAGAAGTGAGTCGAT
GCGGTCGCTGATTCTTTCTA

Product: hypothetical protein

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MASMAGLLKDRGFKITGSDSNPYPPMSTQLESLGISIMKGYKAENLHPKPDFVIVGNVISASNEEAQELMKLGIPYTSLP
KAMGEFIIENRESVVISGTHGKTTTTSMMAWVAENAGVKPGFLIGGIPKNFSQSFKNPEDNYFIIEGDEYDTAFFDKVPK
FIHYRPKHVILTSVEFDHADIYKDLDDVKKSFARLMDLLPENGTLLACAEDANVMELRKLTKCKNSFTYGFGAEADFRAK
IMFQNANGLGFEVHHRGEIMGPYAMQITGEYNVLNATAVVAMSKMLGFSENRIQIALESFEGVKRRQEILGEPNGILVIE
DFAHHPTAVRETVKGIQKKYPGRKVFSVFEPRSATSRRKVFQKDYVEAFKGSHEVLLAKAFDQTKIDEENRFSTHELVDD
LKKTHVTAADFDTADDIVAALKSKAHKGDVILIMSNGGFDGIYTKLLKALE

Sequences:

>Translated_451_residues
MASMAGLLKDRGFKITGSDSNPYPPMSTQLESLGISIMKGYKAENLHPKPDFVIVGNVISASNEEAQELMKLGIPYTSLP
KAMGEFIIENRESVVISGTHGKTTTTSMMAWVAENAGVKPGFLIGGIPKNFSQSFKNPEDNYFIIEGDEYDTAFFDKVPK
FIHYRPKHVILTSVEFDHADIYKDLDDVKKSFARLMDLLPENGTLLACAEDANVMELRKLTKCKNSFTYGFGAEADFRAK
IMFQNANGLGFEVHHRGEIMGPYAMQITGEYNVLNATAVVAMSKMLGFSENRIQIALESFEGVKRRQEILGEPNGILVIE
DFAHHPTAVRETVKGIQKKYPGRKVFSVFEPRSATSRRKVFQKDYVEAFKGSHEVLLAKAFDQTKIDEENRFSTHELVDD
LKKTHVTAADFDTADDIVAALKSKAHKGDVILIMSNGGFDGIYTKLLKALE
>Mature_450_residues
ASMAGLLKDRGFKITGSDSNPYPPMSTQLESLGISIMKGYKAENLHPKPDFVIVGNVISASNEEAQELMKLGIPYTSLPK
AMGEFIIENRESVVISGTHGKTTTTSMMAWVAENAGVKPGFLIGGIPKNFSQSFKNPEDNYFIIEGDEYDTAFFDKVPKF
IHYRPKHVILTSVEFDHADIYKDLDDVKKSFARLMDLLPENGTLLACAEDANVMELRKLTKCKNSFTYGFGAEADFRAKI
MFQNANGLGFEVHHRGEIMGPYAMQITGEYNVLNATAVVAMSKMLGFSENRIQIALESFEGVKRRQEILGEPNGILVIED
FAHHPTAVRETVKGIQKKYPGRKVFSVFEPRSATSRRKVFQKDYVEAFKGSHEVLLAKAFDQTKIDEENRFSTHELVDDL
KKTHVTAADFDTADDIVAALKSKAHKGDVILIMSNGGFDGIYTKLLKALE

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=451, Percent_Identity=37.4722838137472, Blast_Score=319, Evalue=3e-88,
Organism=Escherichia coli, GI1786279, Length=386, Percent_Identity=30.5699481865285, Blast_Score=145, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 50193; Mature: 50062

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASMAGLLKDRGFKITGSDSNPYPPMSTQLESLGISIMKGYKAENLHPKPDFVIVGNVIS
CCCHHHHHHHCCEEEECCCCCCCCCHHHHHHHHCHHHHCCCCCCCCCCCCCEEEEECEEC
ASNEEAQELMKLGIPYTSLPKAMGEFIIENRESVVISGTHGKTTTTSMMAWVAENAGVKP
CCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCC
GFLIGGIPKNFSQSFKNPEDNYFIIEGDEYDTAFFDKVPKFIHYRPKHVILTSVEFDHAD
CEEECCCCCHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECCCHHH
IYKDLDDVKKSFARLMDLLPENGTLLACAEDANVMELRKLTKCKNSFTYGFGAEADFRAK
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCEEE
IMFQNANGLGFEVHHRGEIMGPYAMQITGEYNVLNATAVVAMSKMLGFSENRIQIALESF
EEEECCCCCEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHH
EGVKRRQEILGEPNGILVIEDFAHHPTAVRETVKGIQKKYPGRKVFSVFEPRSATSRRKV
HHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHH
FQKDYVEAFKGSHEVLLAKAFDQTKIDEENRFSTHELVDDLKKTHVTAADFDTADDIVAA
HHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCEECCCCCHHHHHHH
LKSKAHKGDVILIMSNGGFDGIYTKLLKALE
HHHCCCCCCEEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure 
ASMAGLLKDRGFKITGSDSNPYPPMSTQLESLGISIMKGYKAENLHPKPDFVIVGNVIS
CCHHHHHHHCCEEEECCCCCCCCCHHHHHHHHCHHHHCCCCCCCCCCCCCEEEEECEEC
ASNEEAQELMKLGIPYTSLPKAMGEFIIENRESVVISGTHGKTTTTSMMAWVAENAGVKP
CCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCC
GFLIGGIPKNFSQSFKNPEDNYFIIEGDEYDTAFFDKVPKFIHYRPKHVILTSVEFDHAD
CEEECCCCCHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECCCHHH
IYKDLDDVKKSFARLMDLLPENGTLLACAEDANVMELRKLTKCKNSFTYGFGAEADFRAK
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCEEE
IMFQNANGLGFEVHHRGEIMGPYAMQITGEYNVLNATAVVAMSKMLGFSENRIQIALESF
EEEECCCCCEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHH
EGVKRRQEILGEPNGILVIEDFAHHPTAVRETVKGIQKKYPGRKVFSVFEPRSATSRRKV
HHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHH
FQKDYVEAFKGSHEVLLAKAFDQTKIDEENRFSTHELVDDLKKTHVTAADFDTADDIVAA
HHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCEECCCCCHHHHHHH
LKSKAHKGDVILIMSNGGFDGIYTKLLKALE
HHHCCCCCCEEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]