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The map label for this gene is purB [H]

Identifier: 42523426

GI number: 42523426

Start: 1861499

End: 1862755

Strand: Direct

Name: purB [H]

Synonym: Bd1949

Alternate gene names: 42523426

Gene position: 1861499-1862755 (Clockwise)

Preceding gene: 42523425

Following gene: 42523427

Centisome position: 49.21

GC content: 49.8

Gene sequence:

>1257_bases
ATGGGCCTTCTTTGGGACGCCGACCACAAGTTCGGAAAAATGATGGAAGTTGAAATTGCGGTGGCGCAGGTTCAATCCCA
GCTTGGCATCATCCCCAAGAACGCAGCCAAGATCATCGCTCAAAAAGCTCGCTTCAACGTAAAAAGAATCTCTGAAATCG
AACGCGAAACCAAACATGACGTGATCGCCTTTGTTTCAAATCTGGCAGAAAACGTCGGCCCGCAGGGAAAATTCATCCAC
TACGGCATGACGTCTTCGGATGTTCTGGATACGGCCTTCAGCTTGCAGGTGCGCGAAGCCGGAGTCGTTCTGATGAAATC
CATCGTCGCTCTGGAAAAATCCCTGAAAACCCTGGTGACCAAGCATGCGGAAACCCTGTGTGCCGGACGCACCCACGGCA
TGTTTGCCGAGCCGACCACTTTCGGATTCAAGATGGCGGGCTTCCTGGCTGAAGCCCAGCGCAATAAAAAACGTGTGAAA
GCCGCTCTTGATAATATGATGATTTGCAAACTCAGCGGTGCGGTGGGGACTTATTCTTCTCAAGCTCCAAAAATTGAGTC
TTTGGTGGCTAAAAAGCTGAAATTGAAAACTGAAACCATCGCAACTCAAGTGATCCCGCGCGACCGTCATGCCGAGATGC
TTTCAGCCCTGGCTTTGTTCGGAACAGGCCTTGAACGCCTGGCCGTAGAGCTTCGTCATCTGCAGCGCAGTGATGTGGCC
GAAGTGACTGAAGGCTTTACCAAGGGCCAAAAGGGCTCTTCAGCGATGCCTCACAAGAAAAACCCAATCAGTGCGGAAAA
CATAACCGGCCTTTCCCGTTTGCTGCGTGGTTATGCCATTGCCGGATTTGAAAACGTGGCACTTTGGCACGAACGCGACA
TCAGTCATTCCTCAGTGGAGCGCGTGGTGTTCCCGGATGCCTTCATCGTGGCGGACTATGCCTGTAATCGTATGAGCATT
CTGTTGGATGGTCTGGATGTGAACAAGAAGCGCATGATGGACAATATCGAAAGTTCGCAAGGACAGATTTTTAGCTCCCA
TGTGTTGCTGGCGCTGATTCAAAAAGGAATGATTCGTGAGGAAGCGTATGCTTTGGTGCAGCGTCTTTGTCATTCTCTGT
CTTACGGAGAACATCTGAAAGACAAACTTTTGGTGGACGATCAGATCCAGAAGCTTTTGAAGCCCAAAGAGATCGACGAG
ATCTTTACCGGCAAAAAACACAAAAAAGCCATCAAAGACATCATCAAGAGAGTATAG

Upstream 100 bases:

>100_bases
TGCAAATTGTGAACTGGTGGAAAGAAAAACTTCTGAAAGAGCAAAAAGAGAAGAAGCGAAAGGAACAAAATCCGTGATTG
AGAGATACACCCGCCCGGAA

Downstream 100 bases:

>100_bases
ACAATGAATTGGAAGTTTTTGAAAGAAAATGATGTGATTGATGTGGTGGCCCCGGGCTATCCTTCCAAACCTGAAGAGGT
GGAAGGGGCGCGTGCGTTTC

Product: hypothetical protein

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MGLLWDADHKFGKMMEVEIAVAQVQSQLGIIPKNAAKIIAQKARFNVKRISEIERETKHDVIAFVSNLAENVGPQGKFIH
YGMTSSDVLDTAFSLQVREAGVVLMKSIVALEKSLKTLVTKHAETLCAGRTHGMFAEPTTFGFKMAGFLAEAQRNKKRVK
AALDNMMICKLSGAVGTYSSQAPKIESLVAKKLKLKTETIATQVIPRDRHAEMLSALALFGTGLERLAVELRHLQRSDVA
EVTEGFTKGQKGSSAMPHKKNPISAENITGLSRLLRGYAIAGFENVALWHERDISHSSVERVVFPDAFIVADYACNRMSI
LLDGLDVNKKRMMDNIESSQGQIFSSHVLLALIQKGMIREEAYALVQRLCHSLSYGEHLKDKLLVDDQIQKLLKPKEIDE
IFTGKKHKKAIKDIIKRV

Sequences:

>Translated_418_residues
MGLLWDADHKFGKMMEVEIAVAQVQSQLGIIPKNAAKIIAQKARFNVKRISEIERETKHDVIAFVSNLAENVGPQGKFIH
YGMTSSDVLDTAFSLQVREAGVVLMKSIVALEKSLKTLVTKHAETLCAGRTHGMFAEPTTFGFKMAGFLAEAQRNKKRVK
AALDNMMICKLSGAVGTYSSQAPKIESLVAKKLKLKTETIATQVIPRDRHAEMLSALALFGTGLERLAVELRHLQRSDVA
EVTEGFTKGQKGSSAMPHKKNPISAENITGLSRLLRGYAIAGFENVALWHERDISHSSVERVVFPDAFIVADYACNRMSI
LLDGLDVNKKRMMDNIESSQGQIFSSHVLLALIQKGMIREEAYALVQRLCHSLSYGEHLKDKLLVDDQIQKLLKPKEIDE
IFTGKKHKKAIKDIIKRV
>Mature_417_residues
GLLWDADHKFGKMMEVEIAVAQVQSQLGIIPKNAAKIIAQKARFNVKRISEIERETKHDVIAFVSNLAENVGPQGKFIHY
GMTSSDVLDTAFSLQVREAGVVLMKSIVALEKSLKTLVTKHAETLCAGRTHGMFAEPTTFGFKMAGFLAEAQRNKKRVKA
ALDNMMICKLSGAVGTYSSQAPKIESLVAKKLKLKTETIATQVIPRDRHAEMLSALALFGTGLERLAVELRHLQRSDVAE
VTEGFTKGQKGSSAMPHKKNPISAENITGLSRLLRGYAIAGFENVALWHERDISHSSVERVVFPDAFIVADYACNRMSIL
LDGLDVNKKRMMDNIESSQGQIFSSHVLLALIQKGMIREEAYALVQRLCHSLSYGEHLKDKLLVDDQIQKLLKPKEIDEI
FTGKKHKKAIKDIIKRV

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI183227688, Length=389, Percent_Identity=24.6786632390745, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI4557269, Length=384, Percent_Identity=24.4791666666667, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1787376, Length=328, Percent_Identity=28.6585365853659, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790398, Length=379, Percent_Identity=24.5382585751979, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17508577, Length=370, Percent_Identity=25.4054054054054, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI32564234, Length=314, Percent_Identity=27.0700636942675, Blast_Score=95, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6323391, Length=377, Percent_Identity=24.6684350132626, Blast_Score=115, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24647570, Length=401, Percent_Identity=24.9376558603491, Blast_Score=101, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 46433; Mature: 46302

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLLWDADHKFGKMMEVEIAVAQVQSQLGIIPKNAAKIIAQKARFNVKRISEIERETKHD
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
VIAFVSNLAENVGPQGKFIHYGMTSSDVLDTAFSLQVREAGVVLMKSIVALEKSLKTLVT
HHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KHAETLCAGRTHGMFAEPTTFGFKMAGFLAEAQRNKKRVKAALDNMMICKLSGAVGTYSS
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCC
QAPKIESLVAKKLKLKTETIATQVIPRDRHAEMLSALALFGTGLERLAVELRHLQRSDVA
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
EVTEGFTKGQKGSSAMPHKKNPISAENITGLSRLLRGYAIAGFENVALWHERDISHSSVE
HHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCHHHHH
RVVFPDAFIVADYACNRMSILLDGLDVNKKRMMDNIESSQGQIFSSHVLLALIQKGMIRE
HHCCCCHHHHHHHHCCCHHHEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
EAYALVQRLCHSLSYGEHLKDKLLVDDQIQKLLKPKEIDEIFTGKKHKKAIKDIIKRV
HHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
GLLWDADHKFGKMMEVEIAVAQVQSQLGIIPKNAAKIIAQKARFNVKRISEIERETKHD
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
VIAFVSNLAENVGPQGKFIHYGMTSSDVLDTAFSLQVREAGVVLMKSIVALEKSLKTLVT
HHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KHAETLCAGRTHGMFAEPTTFGFKMAGFLAEAQRNKKRVKAALDNMMICKLSGAVGTYSS
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCC
QAPKIESLVAKKLKLKTETIATQVIPRDRHAEMLSALALFGTGLERLAVELRHLQRSDVA
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
EVTEGFTKGQKGSSAMPHKKNPISAENITGLSRLLRGYAIAGFENVALWHERDISHSSVE
HHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCHHHHH
RVVFPDAFIVADYACNRMSILLDGLDVNKKRMMDNIESSQGQIFSSHVLLALIQKGMIRE
HHCCCCHHHHHHHHCCCHHHEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
EAYALVQRLCHSLSYGEHLKDKLLVDDQIQKLLKPKEIDEIFTGKKHKKAIKDIIKRV
HHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]