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The map label for this gene is tilS

Identifier: 42523405

GI number: 42523405

Start: 1839178

End: 1840128

Strand: Direct

Name: tilS

Synonym: Bd1927

Alternate gene names: 42523405

Gene position: 1839178-1840128 (Clockwise)

Preceding gene: 42523404

Following gene: 42523406

Centisome position: 48.62

GC content: 50.16

Gene sequence:

>951_bases
GTGAAACTGTCAAAGGCCAAGCAGGATCTGGATCATCATGTATGGAAGCTGATCAAACTTCATTCGCTTCAGGATAAAAA
GATTCTGGTGGCGCTTTCCGGTGGCACCGATTCGGTGGCTTTGCTTCGCAGTTTGACGAAGGTGCACAAAAAGAATCTGC
TCGGGGCCTGTTATTTTCATCATGGAGAGGACTCGAACCAGGAATACCGCAAAGAAGCCCAAGAGTTTTGTGAAAAGCTT
TGTAAGAAGCTCGAAATTGAATTCTACCCATTGCGCGCCTCGCAATTGGCTAAATCCGAAGCTGAATATCGCGAGCTGCG
TTATGAGGCCTTGGATCGCCTGAAAAAGGAGCAAGGGTTCGAACTTGTGGCCACGGGGCACCATCGGGATGACCTGCTAG
AGACGCGTCTGATTCGTTTGATTCGCGGCACAGGGGCTCAGGGGTTTGCGGCAATGCATGTTTTGCGGGATGGTCTGTTT
CGTCCACTTTTGGAGATTTCCAAGAAAGAGCTTAAAAAGTATCTGCGCGAAGAGCGTCTGCGAAGCTTTGAGGACCCAAC
TAACAAGGCCCTGGACCCTTTGCGTAATTGGCTGCGGGAGGAGTGGCTGAAATCCCTTGAAAGGCGCGCCAGAGGCTCGA
CTGCGGCCTTAGCCCGGTCTTTGGAGACCATTGCTCAAGAGATTGAAAATCGCCCCTGGGGAGATTTGCTGGGGCAGAAT
GAGGCTTATAAGACACAGGGGTTGCGCAGGTCTTTTTACCTCACATTGAGTCCGTTTGAGCAAAAAAGATTGCTGGCTCA
GTACCTGTTTTCCCTCGGGAAACGAGATTTTTCGCAATCTCACCTTGAAGAAATTCAAAAGCGCCTGGACAAGTCGCAAA
AAGTGATCACATTCAAAGTCGCTGGGTGCCAATGGGAAGTTAACGCAGAGCAAATCAAGGTCCAGTCCTGA

Upstream 100 bases:

>100_bases
TTTGGCGGAATCAAAGAATCTGGCTACGGCCGCGAATTGGGTCATTTTGGAATCATGGAATTTGTGAATATCAAAACCGT
CGCGGTAGCGGGGGAGTAGA

Downstream 100 bases:

>100_bases
TTTGAGAGGACTGGTTTCTCACTCTAAAGTTTCTTTTTTTGAGAGGGCATTCATGCTACCCTTTGGGGGTATTACCTTCG
ACTGAAAGGAACTGAAATGC

Product: putative cell cycle protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MKLSKAKQDLDHHVWKLIKLHSLQDKKILVALSGGTDSVALLRSLTKVHKKNLLGACYFHHGEDSNQEYRKEAQEFCEKL
CKKLEIEFYPLRASQLAKSEAEYRELRYEALDRLKKEQGFELVATGHHRDDLLETRLIRLIRGTGAQGFAAMHVLRDGLF
RPLLEISKKELKKYLREERLRSFEDPTNKALDPLRNWLREEWLKSLERRARGSTAALARSLETIAQEIENRPWGDLLGQN
EAYKTQGLRRSFYLTLSPFEQKRLLAQYLFSLGKRDFSQSHLEEIQKRLDKSQKVITFKVAGCQWEVNAEQIKVQS

Sequences:

>Translated_316_residues
MKLSKAKQDLDHHVWKLIKLHSLQDKKILVALSGGTDSVALLRSLTKVHKKNLLGACYFHHGEDSNQEYRKEAQEFCEKL
CKKLEIEFYPLRASQLAKSEAEYRELRYEALDRLKKEQGFELVATGHHRDDLLETRLIRLIRGTGAQGFAAMHVLRDGLF
RPLLEISKKELKKYLREERLRSFEDPTNKALDPLRNWLREEWLKSLERRARGSTAALARSLETIAQEIENRPWGDLLGQN
EAYKTQGLRRSFYLTLSPFEQKRLLAQYLFSLGKRDFSQSHLEEIQKRLDKSQKVITFKVAGCQWEVNAEQIKVQS
>Mature_316_residues
MKLSKAKQDLDHHVWKLIKLHSLQDKKILVALSGGTDSVALLRSLTKVHKKNLLGACYFHHGEDSNQEYRKEAQEFCEKL
CKKLEIEFYPLRASQLAKSEAEYRELRYEALDRLKKEQGFELVATGHHRDDLLETRLIRLIRGTGAQGFAAMHVLRDGLF
RPLLEISKKELKKYLREERLRSFEDPTNKALDPLRNWLREEWLKSLERRARGSTAALARSLETIAQEIENRPWGDLLGQN
EAYKTQGLRRSFYLTLSPFEQKRLLAQYLFSLGKRDFSQSHLEEIQKRLDKSQKVITFKVAGCQWEVNAEQIKVQS

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=216, Percent_Identity=33.7962962962963, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_BDEBA (Q6MLS8)

Other databases:

- EMBL:   BX842651
- RefSeq:   NP_968785.1
- ProteinModelPortal:   Q6MLS8
- SMR:   Q6MLS8
- GeneID:   2735145
- GenomeReviews:   BX842601_GR
- KEGG:   bba:Bd1927
- NMPDR:   fig|264462.1.peg.1756
- OMA:   HALEKYM
- BioCyc:   BBAC264462:BD1927-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 36905; Mature: 36905

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSKAKQDLDHHVWKLIKLHSLQDKKILVALSGGTDSVALLRSLTKVHKKNLLGACYFH
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEE
HGEDSNQEYRKEAQEFCEKLCKKLEIEFYPLRASQLAKSEAEYRELRYEALDRLKKEQGF
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ELVATGHHRDDLLETRLIRLIRGTGAQGFAAMHVLRDGLFRPLLEISKKELKKYLREERL
EEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSFEDPTNKALDPLRNWLREEWLKSLERRARGSTAALARSLETIAQEIENRPWGDLLGQN
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHCCCC
EAYKTQGLRRSFYLTLSPFEQKRLLAQYLFSLGKRDFSQSHLEEIQKRLDKSQKVITFKV
HHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEE
AGCQWEVNAEQIKVQS
ECEEEEECHHHHEECC
>Mature Secondary Structure
MKLSKAKQDLDHHVWKLIKLHSLQDKKILVALSGGTDSVALLRSLTKVHKKNLLGACYFH
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEE
HGEDSNQEYRKEAQEFCEKLCKKLEIEFYPLRASQLAKSEAEYRELRYEALDRLKKEQGF
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ELVATGHHRDDLLETRLIRLIRGTGAQGFAAMHVLRDGLFRPLLEISKKELKKYLREERL
EEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSFEDPTNKALDPLRNWLREEWLKSLERRARGSTAALARSLETIAQEIENRPWGDLLGQN
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHCCCC
EAYKTQGLRRSFYLTLSPFEQKRLLAQYLFSLGKRDFSQSHLEEIQKRLDKSQKVITFKV
HHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEE
AGCQWEVNAEQIKVQS
ECEEEEECHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA