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The map label for this gene is cca [H]

Identifier: 42523306

GI number: 42523306

Start: 1747288

End: 1748481

Strand: Direct

Name: cca [H]

Synonym: Bd1820

Alternate gene names: 42523306

Gene position: 1747288-1748481 (Clockwise)

Preceding gene: 42523304

Following gene: 42523307

Centisome position: 46.19

GC content: 54.61

Gene sequence:

>1194_bases
GTGCAAAGTCTTCTTCAATCCCATCCTCACTGGCCGGCCGTGGAGTCAATCTACCACAGGCTGCAGGCCCATGGCTATAT
GGCCTTTTTGGCCGGCGGCTGTGTGCGCGACGCTTTGCTGGGGGTTCAGGCTCATGATTTGGATCTGGCGACCAATGCCA
CTCCGGAAAAAGTGGAGTCCTTGTTTAGTAAAACCGTCAATGTGGGTAAAAGCTTCGGGGTCATGCGGGTGCTGGTCGAG
GGGGCGGATATCGAGGTCGCCACCTTCCGCAATGACGGCACTTACAGCGACGGTCGCCGGCCTGAGAATGTGGTTTTCTC
AAGCCCCCAAGAGGACGCCCAGCGCCGGGATTTCACCGTGAATGCTCTTTTCTATGATCTGGCGACGGCCCAGGTGCTGG
ATTATGTCGAGGGCCAAAAAGATCTGAAAGCGCGTGTTCTGCGCACGGTGGGGGACGCCGAGCGCCGGTTTGAAGAGGAT
CATCTGCGCCTGCTCAGGGCGGCCCGTTTTTCCGCGCAGTTGAATTTTGAGCTGGAACCGGTCAGTTTTGCCGCCGTAAA
AAAAATGGCTGAAAAAGTGAAGTCTGTCAGCGGCGAGCGCATCCGCGAGGAAATGACGAAGCTTCTGAAAACAGAGCACG
CCGACCATGGACTTCAGGTCATGAAGGATTCTGGTTTGATGAAGGTGCTGTTCCCTTTTCGGCTGCGTAAAAACGACTGG
GTGAAAAACAGTCTGGCCACCGAGGCCTGGCAGCGTCTGGCGATGTTCGTGCGCAGCGCTCAGGGCACCGAGCTTGTTGT
GGTTCTGGATATGCTGAAGCTTTCCACCCGGGATCGTCGCGCGATCGAGGACGCCTGGAAAGTCTGGCAGGATCCTCAAA
CAGTTCTGAAGAACTCTTTGGGAAAACAGCTGCAGATGCTGGCTAAAGAGGGTGTTGTTTTTGCTTTAAGAATTTTACTG
TCCGAAGCGGTTGAAAAGACCGCGATTGAATTCCTGCTGGCTGAATGGAAGAGCTGGCAGGAAGTGCTGCCTCGGCCGTT
TTTAAATGGCGAGGATGTCAAGGGGCGTCTGACTGGGCCTGCTATTGGAGTCTGCCTGGACAAAGCCTTTGAACAGCAGC
TGGAGCGTCAGTTGCAAAGCCGCGATGAAGCTTTGTTGTGGCTGAAAAAATATCTGGAAAGAGAGTCATCTTAA

Upstream 100 bases:

>100_bases
TCAAGGCTTCATCGATAAGAGATTTCATCTCCTGTGGCGTCACGCGAACAAACTCTTTCTTTCAGGCCTGTCTTTTTCTA
CACTCTAGGGTGTGAATCAG

Downstream 100 bases:

>100_bases
ATGGAATCCGCATTTGTTCCCAGCACCTGGAGACGTTTATTTGCCCATGGCATCGATCAGATCATCAGTTTGCCATTTTA
TCTGCCGTTTGCCGGTGTGT

Product: poly A polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MQSLLQSHPHWPAVESIYHRLQAHGYMAFLAGGCVRDALLGVQAHDLDLATNATPEKVESLFSKTVNVGKSFGVMRVLVE
GADIEVATFRNDGTYSDGRRPENVVFSSPQEDAQRRDFTVNALFYDLATAQVLDYVEGQKDLKARVLRTVGDAERRFEED
HLRLLRAARFSAQLNFELEPVSFAAVKKMAEKVKSVSGERIREEMTKLLKTEHADHGLQVMKDSGLMKVLFPFRLRKNDW
VKNSLATEAWQRLAMFVRSAQGTELVVVLDMLKLSTRDRRAIEDAWKVWQDPQTVLKNSLGKQLQMLAKEGVVFALRILL
SEAVEKTAIEFLLAEWKSWQEVLPRPFLNGEDVKGRLTGPAIGVCLDKAFEQQLERQLQSRDEALLWLKKYLERESS

Sequences:

>Translated_397_residues
MQSLLQSHPHWPAVESIYHRLQAHGYMAFLAGGCVRDALLGVQAHDLDLATNATPEKVESLFSKTVNVGKSFGVMRVLVE
GADIEVATFRNDGTYSDGRRPENVVFSSPQEDAQRRDFTVNALFYDLATAQVLDYVEGQKDLKARVLRTVGDAERRFEED
HLRLLRAARFSAQLNFELEPVSFAAVKKMAEKVKSVSGERIREEMTKLLKTEHADHGLQVMKDSGLMKVLFPFRLRKNDW
VKNSLATEAWQRLAMFVRSAQGTELVVVLDMLKLSTRDRRAIEDAWKVWQDPQTVLKNSLGKQLQMLAKEGVVFALRILL
SEAVEKTAIEFLLAEWKSWQEVLPRPFLNGEDVKGRLTGPAIGVCLDKAFEQQLERQLQSRDEALLWLKKYLERESS
>Mature_397_residues
MQSLLQSHPHWPAVESIYHRLQAHGYMAFLAGGCVRDALLGVQAHDLDLATNATPEKVESLFSKTVNVGKSFGVMRVLVE
GADIEVATFRNDGTYSDGRRPENVVFSSPQEDAQRRDFTVNALFYDLATAQVLDYVEGQKDLKARVLRTVGDAERRFEED
HLRLLRAARFSAQLNFELEPVSFAAVKKMAEKVKSVSGERIREEMTKLLKTEHADHGLQVMKDSGLMKVLFPFRLRKNDW
VKNSLATEAWQRLAMFVRSAQGTELVVVLDMLKLSTRDRRAIEDAWKVWQDPQTVLKNSLGKQLQMLAKEGVVFALRILL
SEAVEKTAIEFLLAEWKSWQEVLPRPFLNGEDVKGRLTGPAIGVCLDKAFEQQLERQLQSRDEALLWLKKYLERESS

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=202, Percent_Identity=35.1485148514851, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI87081691, Length=227, Percent_Identity=40.5286343612335, Blast_Score=164, Evalue=7e-42,
Organism=Escherichia coli, GI1789436, Length=215, Percent_Identity=33.953488372093, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71995920, Length=205, Percent_Identity=33.6585365853659, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321016, Length=229, Percent_Identity=31.8777292576419, Blast_Score=103, Evalue=6e-23,
Organism=Drosophila melanogaster, GI281360112, Length=207, Percent_Identity=34.2995169082126, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21357337, Length=207, Percent_Identity=34.2995169082126, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45158; Mature: 45158

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSLLQSHPHWPAVESIYHRLQAHGYMAFLAGGCVRDALLGVQAHDLDLATNATPEKVES
CCCHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
LFSKTVNVGKSFGVMRVLVEGADIEVATFRNDGTYSDGRRPENVVFSSPQEDAQRRDFTV
HHHHHHHHCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCCH
NALFYDLATAQVLDYVEGQKDLKARVLRTVGDAERRFEEDHLRLLRAARFSAQLNFELEP
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECC
VSFAAVKKMAEKVKSVSGERIREEMTKLLKTEHADHGLQVMKDSGLMKVLFPFRLRKNDW
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHCCCHH
VKNSLATEAWQRLAMFVRSAQGTELVVVLDMLKLSTRDRRAIEDAWKVWQDPQTVLKNSL
HHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHH
GKQLQMLAKEGVVFALRILLSEAVEKTAIEFLLAEWKSWQEVLPRPFLNGEDVKGRLTGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCC
AIGVCLDKAFEQQLERQLQSRDEALLWLKKYLERESS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQSLLQSHPHWPAVESIYHRLQAHGYMAFLAGGCVRDALLGVQAHDLDLATNATPEKVES
CCCHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
LFSKTVNVGKSFGVMRVLVEGADIEVATFRNDGTYSDGRRPENVVFSSPQEDAQRRDFTV
HHHHHHHHCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCCH
NALFYDLATAQVLDYVEGQKDLKARVLRTVGDAERRFEEDHLRLLRAARFSAQLNFELEP
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECC
VSFAAVKKMAEKVKSVSGERIREEMTKLLKTEHADHGLQVMKDSGLMKVLFPFRLRKNDW
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHCCCHH
VKNSLATEAWQRLAMFVRSAQGTELVVVLDMLKLSTRDRRAIEDAWKVWQDPQTVLKNSL
HHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHH
GKQLQMLAKEGVVFALRILLSEAVEKTAIEFLLAEWKSWQEVLPRPFLNGEDVKGRLTGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCC
AIGVCLDKAFEQQLERQLQSRDEALLWLKKYLERESS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA