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The map label for this gene is dut

Identifier: 42523060

GI number: 42523060

Start: 1479991

End: 1480440

Strand: Direct

Name: dut

Synonym: Bd1553

Alternate gene names: 42523060

Gene position: 1479991-1480440 (Clockwise)

Preceding gene: 42523059

Following gene: 42523066

Centisome position: 39.12

GC content: 54.67

Gene sequence:

>450_bases
ATGCAAAAACTGGCTGTAAAAATCAAAAAACTCGAAAACTTCCATGGCGATCTGCCTCAGTATCAGTCTTTGGGCGCCAG
TGGTTTCGACGTGCGTGCCCAGCTTGCAGGTCCAGTGGTTTTGAACCCGGGCGAAAGAGCGATGATCCCAACCGGCCTCA
GTTTCGAAATCCCACTGGGTTACGAAATCCAGGCGCGCCCCCGCAGCGGCTGGGCAGCAAAAAGCGGCCTGACTGTCCTG
AACACTCCGGGCACCATCGATGCTGACTATCGCGGCGAAGTAAAAATCATCGTGATCAACCTGGGCAACGAAGCGGTCAC
CATCAACGATCAAGAGCGTTGTGCTCAACTGGTGCTAGCACCCGTAATCCAAGCACAATTCGAACTGGTTAACGAACTAA
GCGACACCGAGCGTGGCGCAGGCGGGTTCGGCTCCACTGGAAGAGCTTAA

Upstream 100 bases:

>100_bases
GATCTGGACAAAGCCGCAGGCGTTCTGCTGGGACCTGAAGTGACCAAGCTTCAGGACTGGTGGCAAGATCTAAAACTGTA
AATTTTGGGAAGGGCATCAT

Downstream 100 bases:

>100_bases
GCGCGACCGTTGAAAAAGGCCCATCTGACTTCGTTGTCGGGCCTTTCGCTTCACTTCGACGTGCAGGAGCACGCCTCCGT
TGCGCGAAAAACCCTCCGCG

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MQKLAVKIKKLENFHGDLPQYQSLGASGFDVRAQLAGPVVLNPGERAMIPTGLSFEIPLGYEIQARPRSGWAAKSGLTVL
NTPGTIDADYRGEVKIIVINLGNEAVTINDQERCAQLVLAPVIQAQFELVNELSDTERGAGGFGSTGRA

Sequences:

>Translated_149_residues
MQKLAVKIKKLENFHGDLPQYQSLGASGFDVRAQLAGPVVLNPGERAMIPTGLSFEIPLGYEIQARPRSGWAAKSGLTVL
NTPGTIDADYRGEVKIIVINLGNEAVTINDQERCAQLVLAPVIQAQFELVNELSDTERGAGGFGSTGRA
>Mature_149_residues
MQKLAVKIKKLENFHGDLPQYQSLGASGFDVRAQLAGPVVLNPGERAMIPTGLSFEIPLGYEIQARPRSGWAAKSGLTVL
NTPGTIDADYRGEVKIIVINLGNEAVTINDQERCAQLVLAPVIQAQFELVNELSDTERGAGGFGSTGRA

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=134, Percent_Identity=38.0597014925373, Blast_Score=91, Evalue=5e-19,
Organism=Homo sapiens, GI4503423, Length=134, Percent_Identity=38.0597014925373, Blast_Score=90, Evalue=8e-19,
Organism=Homo sapiens, GI70906441, Length=134, Percent_Identity=38.0597014925373, Blast_Score=88, Evalue=3e-18,
Organism=Escherichia coli, GI1790071, Length=152, Percent_Identity=42.1052631578947, Blast_Score=111, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71988561, Length=148, Percent_Identity=36.4864864864865, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319729, Length=139, Percent_Identity=35.2517985611511, Blast_Score=86, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24583610, Length=129, Percent_Identity=34.8837209302326, Blast_Score=76, Evalue=8e-15,
Organism=Drosophila melanogaster, GI19921126, Length=129, Percent_Identity=34.8837209302326, Blast_Score=76, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_BDEBA (P61906)

Other databases:

- EMBL:   BX842650
- RefSeq:   NP_968440.1
- ProteinModelPortal:   P61906
- SMR:   P61906
- GeneID:   2735500
- GenomeReviews:   BX842601_GR
- KEGG:   bba:Bd1553
- NMPDR:   fig|264462.1.peg.1410
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- PhylomeDB:   P61906
- BioCyc:   BBAC264462:BD1553-MONOMER
- BRENDA:   3.6.1.23
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 15934; Mature: 15934

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: BINDING 81-81 BINDING 95-95

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLAVKIKKLENFHGDLPQYQSLGASGFDVRAQLAGPVVLNPGERAMIPTGLSFEIPLG
CCHHHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEECCCEEECCCCCEEECCCCEEECCCC
YEIQARPRSGWAAKSGLTVLNTPGTIDADYRGEVKIIVINLGNEAVTINDQERCAQLVLA
CEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCCEEEECCHHHHHHHHHH
PVIQAQFELVNELSDTERGAGGFGSTGRA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MQKLAVKIKKLENFHGDLPQYQSLGASGFDVRAQLAGPVVLNPGERAMIPTGLSFEIPLG
CCHHHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEECCCEEECCCCCEEECCCCEEECCCC
YEIQARPRSGWAAKSGLTVLNTPGTIDADYRGEVKIIVINLGNEAVTINDQERCAQLVLA
CEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCCEEEECCHHHHHHHHHH
PVIQAQFELVNELSDTERGAGGFGSTGRA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA