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The map label for this gene is pnp

Identifier: 42523058

GI number: 42523058

Start: 1476606

End: 1478699

Strand: Direct

Name: pnp

Synonym: Bd1551

Alternate gene names: 42523058

Gene position: 1476606-1478699 (Clockwise)

Preceding gene: 42523057

Following gene: 42523059

Centisome position: 39.03

GC content: 49.71

Gene sequence:

>2094_bases
ATGAAAACGACTGTCACAACATCGGTGGGTGGAAAACAAATTACCATCGAAACAGGCCGTTTGGCGAAACAAGCGGATGG
CGCCGCTTTGGTAAGCTCCGGTAACAACATGGTTCTTGTTACTGCAACTTCCTCAAAAAAAGCCTCTGAACTGGATTTCT
TCCCTCTGACTGTTGAGTACATTGAGAAGTTCTACGCAACAGGCAAAATCCCAGGTGGTTACTTCAAACGTGAAGCAAAA
CCAACCAACGACGCGGTTCTGATCGCTCGTCTTATCGACCGTCCAATCCGCCCTGTATTCCCTGAAGGCTACCGCCACGA
AACACAAATCGTTGCGACGGTGTTGTCTGCGGATGGCGCATTCCCACTGGAAATTCTAGCGAGCCTTGGTGCATCCGCGG
CTTTGCATTGCTCTGACATTCCATTCAACGGCCCGACAGCAGCTGTGCAAATCGCACGCGTTGACGGTCAGTTCGTTGCC
AATCCAACTCCACAGCAGATGGAAAAATCTGACATGGACATGATCGTTGCGGGTACTCGCAATGGTTTGTTGATGGTTGA
AGGTGAAACGAAATTCATCTCTGAAGCAGACGCCTTGGCAGCCTTGAAATTCGGTCACCAGTCTTTGATTCCTCTTCTGA
ACGCACAGGATGAGTTGCGTGAAAAAGCCGGTTCTGTGGCGAAACGCGCTTTCACTGCTCCATCCATCGATGCAGACTTC
AAAGCCAAAGCTGAAAGCCTGTTGAGACCTAAAATTGCGGCGGCTCTTTCCATCAAGGAAAAACAAGACCGTTATGCAGC
TGCCAACGAAGCAGCAGCAGAAGCTGAAAAAGCTCTTCTTGCTGACATCGCTGACAAAGAGCTTTTGAAGCAGCGTAAAA
AAGAACTGAACTCTATCGTTGAAGATCTGAAATACCAGGAAGCTCGTTCCATGATTCTGGATCGCGCTGTTCGTATCGAC
GGTCGTGACGTGAAAACTGTTCGTCCTATCGCGAACGAAGTGGGTATCCTGCCTCGCGCTCACGGTTCCGGCTTGTTCAC
CCGTGGTGAAACTCAAGTATTGGGCACTGTGACTTTGGGTACGGCTGATGACGAACAAATGGTGGATTCACTGTTGGGTC
TTCAGAAGCGCAAGTTCATGCTTCACTACAACTTCCCTCCATACTCTGTGGGTGAAGTGGGTCGTATGTCCGGTACCAGC
CGTCGTGAGATCGGTCACGGTAACCTGGCTGAGCGCGCGATCAAAGCGGTTCTTCCAGACTTCGAAAAATTCCCTTACAC
AATCCGTATCGTGTCTGAAGTTTTGGAATCCAATGGTTCCTCTTCCATGGGTACTGTTTGCTCCGGTATCATGGCATTGC
TTGACGCCGGTGTTCCTTTGAAAGGCAACGTTGCCGGTGTGGCAATGGGGCTGATCAAGGAAGGTGACCGCGTTGCGGTT
CTGACGGACATCCTTGGTGACGAAGATCACCTGGGTGACATGGACTTCAAAGTGGCGGGTTCTCCTGCGGGTATCACTGC
TTTGCAGATGGATATCAAAATTGACTCTGTTTCCTTCGAAGTGATGGAGCAGGCTTTGGCGCAAGCTAAAGAAGGCCGCG
CTCACATCCTGAACGAAATGGAAAAAGTGATGAAGGTTCCTCGTGGTCAGATCTCTGAATTTGCACCTCGTATCGAAACC
ATCAAAATCAAGCCAGACAAGATCCGTGAAGTGATCGGCTCCGGTGGTAAAGTGATCCGTGGTATCACTGAAGCTACTGG
CGTTAAGATCGAGATCCAGGACGACGGTACTATCAACATCGCGTCTGCTGATCCGGAAGCAACCAAGAAAGCAATTGCAA
TGATCAACGACATCATCGCTGAAGCTGAAGTGGGCAAGACCTACAAAGGCCGCATCGTGAAAATCGCTGAGTTCGGTGCT
TTCGTTGAAATCCTTCCGAACACACAAGGTCTTTTGCACATCTCTGAAATCTCCAACGAACGTGTACGCGCAGTTTCTGA
CGTACTAAAAGAAGGTGAAATCATCGATGTGAAAGTTCTTGAAGTGGACCGTTCTGGTCGCGTCAAGCTTTCCCGCAAGG
CTTTGCTACAGTAA

Upstream 100 bases:

>100_bases
TCAAAAGATTCTAAATGCAATGAAGGGGCGCAGAGCCCCTTCGTCGTTTCAAGCCACAGCAGCAGAGCATAAGGCTCTGT
GATAACTTCAAGGAGATTTA

Downstream 100 bases:

>100_bases
TAGATGAATACAAAGTTCAAAAAATCTGAACTTTCTAACGGGATCCGGGTGGTGAGTGAACTCCATCCAGGGTCCCGTGC
CGTTTCTATGGGTATTTGGG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 697; Mature: 697

Protein sequence:

>697_residues
MKTTVTTSVGGKQITIETGRLAKQADGAALVSSGNNMVLVTATSSKKASELDFFPLTVEYIEKFYATGKIPGGYFKREAK
PTNDAVLIARLIDRPIRPVFPEGYRHETQIVATVLSADGAFPLEILASLGASAALHCSDIPFNGPTAAVQIARVDGQFVA
NPTPQQMEKSDMDMIVAGTRNGLLMVEGETKFISEADALAALKFGHQSLIPLLNAQDELREKAGSVAKRAFTAPSIDADF
KAKAESLLRPKIAAALSIKEKQDRYAAANEAAAEAEKALLADIADKELLKQRKKELNSIVEDLKYQEARSMILDRAVRID
GRDVKTVRPIANEVGILPRAHGSGLFTRGETQVLGTVTLGTADDEQMVDSLLGLQKRKFMLHYNFPPYSVGEVGRMSGTS
RREIGHGNLAERAIKAVLPDFEKFPYTIRIVSEVLESNGSSSMGTVCSGIMALLDAGVPLKGNVAGVAMGLIKEGDRVAV
LTDILGDEDHLGDMDFKVAGSPAGITALQMDIKIDSVSFEVMEQALAQAKEGRAHILNEMEKVMKVPRGQISEFAPRIET
IKIKPDKIREVIGSGGKVIRGITEATGVKIEIQDDGTINIASADPEATKKAIAMINDIIAEAEVGKTYKGRIVKIAEFGA
FVEILPNTQGLLHISEISNERVRAVSDVLKEGEIIDVKVLEVDRSGRVKLSRKALLQ

Sequences:

>Translated_697_residues
MKTTVTTSVGGKQITIETGRLAKQADGAALVSSGNNMVLVTATSSKKASELDFFPLTVEYIEKFYATGKIPGGYFKREAK
PTNDAVLIARLIDRPIRPVFPEGYRHETQIVATVLSADGAFPLEILASLGASAALHCSDIPFNGPTAAVQIARVDGQFVA
NPTPQQMEKSDMDMIVAGTRNGLLMVEGETKFISEADALAALKFGHQSLIPLLNAQDELREKAGSVAKRAFTAPSIDADF
KAKAESLLRPKIAAALSIKEKQDRYAAANEAAAEAEKALLADIADKELLKQRKKELNSIVEDLKYQEARSMILDRAVRID
GRDVKTVRPIANEVGILPRAHGSGLFTRGETQVLGTVTLGTADDEQMVDSLLGLQKRKFMLHYNFPPYSVGEVGRMSGTS
RREIGHGNLAERAIKAVLPDFEKFPYTIRIVSEVLESNGSSSMGTVCSGIMALLDAGVPLKGNVAGVAMGLIKEGDRVAV
LTDILGDEDHLGDMDFKVAGSPAGITALQMDIKIDSVSFEVMEQALAQAKEGRAHILNEMEKVMKVPRGQISEFAPRIET
IKIKPDKIREVIGSGGKVIRGITEATGVKIEIQDDGTINIASADPEATKKAIAMINDIIAEAEVGKTYKGRIVKIAEFGA
FVEILPNTQGLLHISEISNERVRAVSDVLKEGEIIDVKVLEVDRSGRVKLSRKALLQ
>Mature_697_residues
MKTTVTTSVGGKQITIETGRLAKQADGAALVSSGNNMVLVTATSSKKASELDFFPLTVEYIEKFYATGKIPGGYFKREAK
PTNDAVLIARLIDRPIRPVFPEGYRHETQIVATVLSADGAFPLEILASLGASAALHCSDIPFNGPTAAVQIARVDGQFVA
NPTPQQMEKSDMDMIVAGTRNGLLMVEGETKFISEADALAALKFGHQSLIPLLNAQDELREKAGSVAKRAFTAPSIDADF
KAKAESLLRPKIAAALSIKEKQDRYAAANEAAAEAEKALLADIADKELLKQRKKELNSIVEDLKYQEARSMILDRAVRID
GRDVKTVRPIANEVGILPRAHGSGLFTRGETQVLGTVTLGTADDEQMVDSLLGLQKRKFMLHYNFPPYSVGEVGRMSGTS
RREIGHGNLAERAIKAVLPDFEKFPYTIRIVSEVLESNGSSSMGTVCSGIMALLDAGVPLKGNVAGVAMGLIKEGDRVAV
LTDILGDEDHLGDMDFKVAGSPAGITALQMDIKIDSVSFEVMEQALAQAKEGRAHILNEMEKVMKVPRGQISEFAPRIET
IKIKPDKIREVIGSGGKVIRGITEATGVKIEIQDDGTINIASADPEATKKAIAMINDIIAEAEVGKTYKGRIVKIAEFGA
FVEILPNTQGLLHISEISNERVRAVSDVLKEGEIIDVKVLEVDRSGRVKLSRKALLQ

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=722, Percent_Identity=37.5346260387812, Blast_Score=454, Evalue=1e-127,
Organism=Escherichia coli, GI145693187, Length=685, Percent_Identity=51.0948905109489, Blast_Score=692, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=710, Percent_Identity=34.5070422535211, Blast_Score=345, Evalue=4e-95,
Organism=Caenorhabditis elegans, GI17535281, Length=79, Percent_Identity=46.8354430379747, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI281362905, Length=712, Percent_Identity=35.3932584269663, Blast_Score=427, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24651641, Length=712, Percent_Identity=35.3932584269663, Blast_Score=427, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24651643, Length=712, Percent_Identity=35.3932584269663, Blast_Score=427, Evalue=1e-119,
Organism=Drosophila melanogaster, GI161079377, Length=657, Percent_Identity=35.0076103500761, Blast_Score=394, Evalue=1e-109,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_BDEBA (Q6MMS2)

Other databases:

- EMBL:   BX842650
- RefSeq:   NP_968438.1
- HSSP:   P05055
- ProteinModelPortal:   Q6MMS2
- GeneID:   2735498
- GenomeReviews:   BX842601_GR
- KEGG:   bba:Bd1551
- NMPDR:   fig|264462.1.peg.1408
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   Q6MMS2
- ProtClustDB:   PRK11824
- BioCyc:   BBAC264462:BD1551-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 75122; Mature: 75122

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTVTTSVGGKQITIETGRLAKQADGAALVSSGNNMVLVTATSSKKASELDFFPLTVEY
CCCEEEECCCCEEEEEECCCCHHHCCCCEEEECCCCEEEEEECCCCCCCCCCCCCHHHHH
IEKFYATGKIPGGYFKREAKPTNDAVLIARLIDRPIRPVFPEGYRHETQIVATVLSADGA
HHHHHHCCCCCCCHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCC
FPLEILASLGASAALHCSDIPFNGPTAAVQIARVDGQFVANPTPQQMEKSDMDMIVAGTR
CHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEEECCEEECCCCHHHHHHCCCCEEEEECC
NGLLMVEGETKFISEADALAALKFGHQSLIPLLNAQDELREKAGSVAKRAFTAPSIDADF
CCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCH
KAKAESLLRPKIAAALSIKEKQDRYAAANEAAAEAEKALLADIADKELLKQRKKELNSIV
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDLKYQEARSMILDRAVRIDGRDVKTVRPIANEVGILPRAHGSGLFTRGETQVLGTVTLG
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCEECCCCEEEEEEEEC
TADDEQMVDSLLGLQKRKFMLHYNFPPYSVGEVGRMSGTSRREIGHGNLAERAIKAVLPD
CCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
FEKFPYTIRIVSEVLESNGSSSMGTVCSGIMALLDAGVPLKGNVAGVAMGLIKEGDRVAV
HHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEE
LTDILGDEDHLGDMDFKVAGSPAGITALQMDIKIDSVSFEVMEQALAQAKEGRAHILNEM
EEECCCCCCCCCCCCEEECCCCCCEEEEEEEEEEECHHHHHHHHHHHHHHCCHHHHHHHH
EKVMKVPRGQISEFAPRIETIKIKPDKIREVIGSGGKVIRGITEATGVKIEIQDDGTINI
HHHHHCCCCHHHHHCCCCEEEEECHHHHHHHHCCCCCEEECHHHHCCEEEEEECCCEEEE
ASADPEATKKAIAMINDIIAEAEVGKTYKGRIVKIAEFGAFVEILPNTQGLLHISEISNE
ECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCCCEEEEHHHCCH
RVRAVSDVLKEGEIIDVKVLEVDRSGRVKLSRKALLQ
HHHHHHHHHHCCCEEEEEEEEECCCCCEEEHHHHHCC
>Mature Secondary Structure
MKTTVTTSVGGKQITIETGRLAKQADGAALVSSGNNMVLVTATSSKKASELDFFPLTVEY
CCCEEEECCCCEEEEEECCCCHHHCCCCEEEECCCCEEEEEECCCCCCCCCCCCCHHHHH
IEKFYATGKIPGGYFKREAKPTNDAVLIARLIDRPIRPVFPEGYRHETQIVATVLSADGA
HHHHHHCCCCCCCHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCC
FPLEILASLGASAALHCSDIPFNGPTAAVQIARVDGQFVANPTPQQMEKSDMDMIVAGTR
CHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEEECCEEECCCCHHHHHHCCCCEEEEECC
NGLLMVEGETKFISEADALAALKFGHQSLIPLLNAQDELREKAGSVAKRAFTAPSIDADF
CCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCH
KAKAESLLRPKIAAALSIKEKQDRYAAANEAAAEAEKALLADIADKELLKQRKKELNSIV
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDLKYQEARSMILDRAVRIDGRDVKTVRPIANEVGILPRAHGSGLFTRGETQVLGTVTLG
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCEECCCCEEEEEEEEC
TADDEQMVDSLLGLQKRKFMLHYNFPPYSVGEVGRMSGTSRREIGHGNLAERAIKAVLPD
CCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
FEKFPYTIRIVSEVLESNGSSSMGTVCSGIMALLDAGVPLKGNVAGVAMGLIKEGDRVAV
HHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEE
LTDILGDEDHLGDMDFKVAGSPAGITALQMDIKIDSVSFEVMEQALAQAKEGRAHILNEM
EEECCCCCCCCCCCCEEECCCCCCEEEEEEEEEEECHHHHHHHHHHHHHHCCHHHHHHHH
EKVMKVPRGQISEFAPRIETIKIKPDKIREVIGSGGKVIRGITEATGVKIEIQDDGTINI
HHHHHCCCCHHHHHCCCCEEEEECHHHHHHHHCCCCCEEECHHHHCCEEEEEECCCEEEE
ASADPEATKKAIAMINDIIAEAEVGKTYKGRIVKIAEFGAFVEILPNTQGLLHISEISNE
ECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCCCEEEEHHHCCH
RVRAVSDVLKEGEIIDVKVLEVDRSGRVKLSRKALLQ
HHHHHHHHHHCCCEEEEEEEEECCCCCEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA