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The map label for this gene is truB

Identifier: 42523056

GI number: 42523056

Start: 1475179

End: 1476105

Strand: Direct

Name: truB

Synonym: Bd1549

Alternate gene names: 42523056

Gene position: 1475179-1476105 (Clockwise)

Preceding gene: 42523055

Following gene: 42523057

Centisome position: 39.0

GC content: 54.8

Gene sequence:

>927_bases
ATGACGAATAAAAATCAATTCAATGGTCTGCTGCTGGTGGATAAACCTTCAGGGATCTCCAGCCATGACGTTGTGGCGCG
TTTGCGCCGCATTCTTTCCACGCGCGCTGTGGGGCATTCCGGCACTCTGGATCCCATGGCTTCGGGTCTGATGGCCTGTC
TGGTGAACGAGGGCACAAAACTTAGCCAATACATTCTGGAAGGCGACAAGGGCTACCGCCTGCGTGCGCAGTTCGGGATT
CGCACCGACACCCTCGACACGACCGGCGAAACTTTGGAAACCCGTCCCACGGATCATTTGACCCGGGAACTGATCCTGTC
CGAGGCCCTCAAGCTTCAGGGCGAGATGGAAGTTGAAGTGCCTATTTACTCGGCAATCAAGGTTCAGGGTAAAAAGCTTT
ATGAATACGCCCGGGGCGAGCAGGAAGTGACCATTCCCAAGAAGGTCATGAAGTTCTGGGATATTGAACCTGTGGAAATC
GGCTCTGATTGGGCAGAATTTGATATCAAATGCAGTAAGGGCAGCTATATCCGCACCTGGATCGACCTGCTGGGTAAGGC
CTTGGGGTGTGGGGCGGCGATGAGCGGCCTGCGCCGGACGTGGTCTTCACCGTATAAAATTGACCAGGCTCAGACTCTGG
AGCAGATCGAGGCTTCGGTCAAAGGCGGCTCTTTAGGCCCTGCTTTTGTTCCCATGGAGCTGGCGCTGCCTCAGGTGAAA
CGTATTCGAATCAAGGGGCAAGACAAGGTCCTTTTGGGGAATGGCCAGATCAGCCATGACCTCAGATCCCAGCTTATTTC
GGCCTTCAATCCCGATGTGGACCAGTACATTCAGGTTCTGGCCCAAGAGGGGGGCGAGCTTTTGGCCGTTATTGGCCTGG
AGCCCGGCCGGGGGTTCGTTCTACGCAGGGTATTTAAATACTCTTGA

Upstream 100 bases:

>100_bases
GATCACGCCACCGAGCAGGTGCTGAAGGTTGAAAAAATCCTTCACGAGCTTGAGCTTGAGCGCAAGGCCAATAACCCTGG
CGAAGGTTCCGACGAATCCG

Downstream 100 bases:

>100_bases
CCCGTTTGGGGCTTGGGTTTAAACCATAACTTCACAAAAAATCTCTCTGGAGCGACAGCCATGACGCCCGAGGGGGATGT
GATAACTAAAACCATTTCAG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTNKNQFNGLLLVDKPSGISSHDVVARLRRILSTRAVGHSGTLDPMASGLMACLVNEGTKLSQYILEGDKGYRLRAQFGI
RTDTLDTTGETLETRPTDHLTRELILSEALKLQGEMEVEVPIYSAIKVQGKKLYEYARGEQEVTIPKKVMKFWDIEPVEI
GSDWAEFDIKCSKGSYIRTWIDLLGKALGCGAAMSGLRRTWSSPYKIDQAQTLEQIEASVKGGSLGPAFVPMELALPQVK
RIRIKGQDKVLLGNGQISHDLRSQLISAFNPDVDQYIQVLAQEGGELLAVIGLEPGRGFVLRRVFKYS

Sequences:

>Translated_308_residues
MTNKNQFNGLLLVDKPSGISSHDVVARLRRILSTRAVGHSGTLDPMASGLMACLVNEGTKLSQYILEGDKGYRLRAQFGI
RTDTLDTTGETLETRPTDHLTRELILSEALKLQGEMEVEVPIYSAIKVQGKKLYEYARGEQEVTIPKKVMKFWDIEPVEI
GSDWAEFDIKCSKGSYIRTWIDLLGKALGCGAAMSGLRRTWSSPYKIDQAQTLEQIEASVKGGSLGPAFVPMELALPQVK
RIRIKGQDKVLLGNGQISHDLRSQLISAFNPDVDQYIQVLAQEGGELLAVIGLEPGRGFVLRRVFKYS
>Mature_307_residues
TNKNQFNGLLLVDKPSGISSHDVVARLRRILSTRAVGHSGTLDPMASGLMACLVNEGTKLSQYILEGDKGYRLRAQFGIR
TDTLDTTGETLETRPTDHLTRELILSEALKLQGEMEVEVPIYSAIKVQGKKLYEYARGEQEVTIPKKVMKFWDIEPVEIG
SDWAEFDIKCSKGSYIRTWIDLLGKALGCGAAMSGLRRTWSSPYKIDQAQTLEQIEASVKGGSLGPAFVPMELALPQVKR
IRIKGQDKVLLGNGQISHDLRSQLISAFNPDVDQYIQVLAQEGGELLAVIGLEPGRGFVLRRVFKYS

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=268, Percent_Identity=29.4776119402985, Blast_Score=105, Evalue=5e-23,
Organism=Escherichia coli, GI2367200, Length=259, Percent_Identity=37.4517374517375, Blast_Score=174, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17553978, Length=248, Percent_Identity=27.4193548387097, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324037, Length=170, Percent_Identity=30.5882352941176, Blast_Score=79, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323204, Length=247, Percent_Identity=28.3400809716599, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281364189, Length=195, Percent_Identity=28.7179487179487, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI281364187, Length=195, Percent_Identity=28.7179487179487, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI281364185, Length=195, Percent_Identity=28.7179487179487, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI281364183, Length=195, Percent_Identity=28.7179487179487, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI62471759, Length=195, Percent_Identity=28.7179487179487, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17975520, Length=195, Percent_Identity=28.7179487179487, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_BDEBA (Q6MMS4)

Other databases:

- EMBL:   BX842650
- RefSeq:   NP_968436.1
- ProteinModelPortal:   Q6MMS4
- SMR:   Q6MMS4
- GeneID:   2735496
- GenomeReviews:   BX842601_GR
- KEGG:   bba:Bd1549
- NMPDR:   fig|264462.1.peg.1406
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- PhylomeDB:   Q6MMS4
- BioCyc:   BBAC264462:BD1549-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 34111; Mature: 33980

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: ACT_SITE 44-44

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKNQFNGLLLVDKPSGISSHDVVARLRRILSTRAVGHSGTLDPMASGLMACLVNEGTK
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCH
LSQYILEGDKGYRLRAQFGIRTDTLDTTGETLETRPTDHLTRELILSEALKLQGEMEVEV
HHHHHHCCCCCEEEEEEECCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEE
PIYSAIKVQGKKLYEYARGEQEVTIPKKVMKFWDIEPVEIGSDWAEFDIKCSKGSYIRTW
EHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEECCCCHHHEEEEECCCCHHHHH
IDLLGKALGCGAAMSGLRRTWSSPYKIDQAQTLEQIEASVKGGSLGPAFVPMELALPQVK
HHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCEE
RIRIKGQDKVLLGNGQISHDLRSQLISAFNPDVDQYIQVLAQEGGELLAVIGLEPGRGFV
EEEECCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCHHH
LRRVFKYS
HHHHHHCC
>Mature Secondary Structure 
TNKNQFNGLLLVDKPSGISSHDVVARLRRILSTRAVGHSGTLDPMASGLMACLVNEGTK
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCH
LSQYILEGDKGYRLRAQFGIRTDTLDTTGETLETRPTDHLTRELILSEALKLQGEMEVEV
HHHHHHCCCCCEEEEEEECCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEE
PIYSAIKVQGKKLYEYARGEQEVTIPKKVMKFWDIEPVEIGSDWAEFDIKCSKGSYIRTW
EHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEECCCCHHHEEEEECCCCHHHHH
IDLLGKALGCGAAMSGLRRTWSSPYKIDQAQTLEQIEASVKGGSLGPAFVPMELALPQVK
HHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCEE
RIRIKGQDKVLLGNGQISHDLRSQLISAFNPDVDQYIQVLAQEGGELLAVIGLEPGRGFV
EEEECCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCHHH
LRRVFKYS
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA