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The map label for this gene is nusA [H]

Identifier: 42523053

GI number: 42523053

Start: 1470368

End: 1471756

Strand: Direct

Name: nusA [H]

Synonym: Bd1546

Alternate gene names: 42523053

Gene position: 1470368-1471756 (Clockwise)

Preceding gene: 42523052

Following gene: 42523054

Centisome position: 38.87

GC content: 49.6

Gene sequence:

>1389_bases
ATGGCTGAAAATGTGTTTTCAGATCTTTCCAAGGTGATTGATCAAGTCGGAAAAGACAAAGGTATCGACAAACAGGTTGT
TATCGATGCAATCACTCAGGGTATGCTGGTAGCTGCACGTAAAAAATACGGTACTTACCGCGAGATCGAAGCTGCATACA
ACGAAGACACCGGCGAAGTTGAACTGTTTGAGTTCAAAGAAGTTGTTCCTCGTGAAAAATACATCGATGAAGAAGTTGAG
ATTCCTCTTGATGAAGCTCAGAAGCTGGATCCAAATGTTCAGCTGGATGACTCCATTGGTATCAAAATGGAAGCTTCCGA
CCTGGGCCGTATCGCTGCTCAGACTGCAAAACAAATCATCATGCAGAAAGTGCGTGATGCGGAACGCAACATCATCTTCA
ATGAATTTGAAGAGCGCAAAGGCGAGATCGCTTCCGGTATCGCACGTCGTGTTGAAAAAGGCGCGATCGTTGTCGATTTG
GGCCGCACTGAGGCTTACATTCCGCCTCGTGAACAGATCCCGGGCGAACAATACAAGCCAGGCGATCGCATTCAGGGTTA
CCTTTCTGAAGTTCGTCAGACGACACGTGGTCCTCAGATCATCATGTCCCGCGCTGATGAACGTTATTTGATGAAACTTT
TCGAAATGGAAGTTCCAGAGATCTACGACGGTGTTGTGGAAATCATGGCAGCCGCTCGTGAACCAGGACAGCGCGCGAAA
ATCGCGGTTCGTTCCAAAGACAACTCCGTGGACCCGGTAGGGGCTTGCGTGGGTATGAAGGGTTCCCGTGTTCAGAATAT
CGTTCAGGAGCTTCGTGGCGAGAAAATCGACATCGTTCCTTGGGACGAGGATATCACTCGTTTCGCGTGCAACGCTTTGG
CGCCTGCGGAAATCTCCCGCGTGTTCCTGGATGATGCCAACCGCGAAATGGAAATCGTTGTTCCGGACTCTCAATTGTCT
TTGGCTATCGGTAAACGTGGTCAAAACGTGCGTCTGGCAGCGAAACTGACAACTTGGAAGCTGGATATCATTTCTGAATC
TTCAGCGGCTTCCCGCACAGCGGAATCCATCTTCAATCTGATGTTGATCCCGGGCATGAGCGAAACCATGGCTCAGAACA
TCTTCCAGTCCGGCTTCGGTTCATTCCAGGCTGTGGCGACGGCGGCTGTTGAAGAGCTGATGACAATCCCTGGTTACGAC
GATCCAGACAAAGCCGAAAAGCTTTCCAAAGAAGCCAAAGGCCTGGTTGCGAAGTATGAATCTGAAGGTGTGCCGGTTCC
AACCGCTCCTTCAGTTGCCAAGGACAAAGGTTCCAACGTGTCTGCAAAAGAGCAGGCCGATCTTCTGTTGAAGCAAGAGT
TGAAAAAACTCGAAGCTGAAGAAGAGTAG

Upstream 100 bases:

>100_bases
GAAGGACGTGGAGATCAAAATCCCGTACGCGATGATCGACAGGGCAAAATTGGTTTTTGAATATACTAAAGGTCAGAAAA
AGTAACGAGAGAGGAAAATC

Downstream 100 bases:

>100_bases
TATTTAGTTATTTGGATAGAGAACAACAACGCCGCCCCGGCAAAGTATCGGGGTGAGGCAGGCTGCCCCGGAAAGTTCCG
GGGTCTAAGCAGGGAGACTG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MAENVFSDLSKVIDQVGKDKGIDKQVVIDAITQGMLVAARKKYGTYREIEAAYNEDTGEVELFEFKEVVPREKYIDEEVE
IPLDEAQKLDPNVQLDDSIGIKMEASDLGRIAAQTAKQIIMQKVRDAERNIIFNEFEERKGEIASGIARRVEKGAIVVDL
GRTEAYIPPREQIPGEQYKPGDRIQGYLSEVRQTTRGPQIIMSRADERYLMKLFEMEVPEIYDGVVEIMAAAREPGQRAK
IAVRSKDNSVDPVGACVGMKGSRVQNIVQELRGEKIDIVPWDEDITRFACNALAPAEISRVFLDDANREMEIVVPDSQLS
LAIGKRGQNVRLAAKLTTWKLDIISESSAASRTAESIFNLMLIPGMSETMAQNIFQSGFGSFQAVATAAVEELMTIPGYD
DPDKAEKLSKEAKGLVAKYESEGVPVPTAPSVAKDKGSNVSAKEQADLLLKQELKKLEAEEE

Sequences:

>Translated_462_residues
MAENVFSDLSKVIDQVGKDKGIDKQVVIDAITQGMLVAARKKYGTYREIEAAYNEDTGEVELFEFKEVVPREKYIDEEVE
IPLDEAQKLDPNVQLDDSIGIKMEASDLGRIAAQTAKQIIMQKVRDAERNIIFNEFEERKGEIASGIARRVEKGAIVVDL
GRTEAYIPPREQIPGEQYKPGDRIQGYLSEVRQTTRGPQIIMSRADERYLMKLFEMEVPEIYDGVVEIMAAAREPGQRAK
IAVRSKDNSVDPVGACVGMKGSRVQNIVQELRGEKIDIVPWDEDITRFACNALAPAEISRVFLDDANREMEIVVPDSQLS
LAIGKRGQNVRLAAKLTTWKLDIISESSAASRTAESIFNLMLIPGMSETMAQNIFQSGFGSFQAVATAAVEELMTIPGYD
DPDKAEKLSKEAKGLVAKYESEGVPVPTAPSVAKDKGSNVSAKEQADLLLKQELKKLEAEEE
>Mature_461_residues
AENVFSDLSKVIDQVGKDKGIDKQVVIDAITQGMLVAARKKYGTYREIEAAYNEDTGEVELFEFKEVVPREKYIDEEVEI
PLDEAQKLDPNVQLDDSIGIKMEASDLGRIAAQTAKQIIMQKVRDAERNIIFNEFEERKGEIASGIARRVEKGAIVVDLG
RTEAYIPPREQIPGEQYKPGDRIQGYLSEVRQTTRGPQIIMSRADERYLMKLFEMEVPEIYDGVVEIMAAAREPGQRAKI
AVRSKDNSVDPVGACVGMKGSRVQNIVQELRGEKIDIVPWDEDITRFACNALAPAEISRVFLDDANREMEIVVPDSQLSL
AIGKRGQNVRLAAKLTTWKLDIISESSAASRTAESIFNLMLIPGMSETMAQNIFQSGFGSFQAVATAAVEELMTIPGYDD
PDKAEKLSKEAKGLVAKYESEGVPVPTAPSVAKDKGSNVSAKEQADLLLKQELKKLEAEEE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=413, Percent_Identity=37.2881355932203, Blast_Score=282, Evalue=3e-77,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 51178; Mature: 51047

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAENVFSDLSKVIDQVGKDKGIDKQVVIDAITQGMLVAARKKYGTYREIEAAYNEDTGEV
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCE
ELFEFKEVVPREKYIDEEVEIPLDEAQKLDPNVQLDDSIGIKMEASDLGRIAAQTAKQII
EEHHHHHHCCHHHHCCCCCCCCHHHHHCCCCCCEECCCCCCEEEHHHHHHHHHHHHHHHH
MQKVRDAERNIIFNEFEERKGEIASGIARRVEKGAIVVDLGRTEAYIPPREQIPGEQYKP
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHCCCCCCCC
GDRIQGYLSEVRQTTRGPQIIMSRADERYLMKLFEMEVPEIYDGVVEIMAAAREPGQRAK
HHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCE
IAVRSKDNSVDPVGACVGMKGSRVQNIVQELRGEKIDIVPWDEDITRFACNALAPAEISR
EEEECCCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCHHHHHH
VFLDDANREMEIVVPDSQLSLAIGKRGQNVRLAAKLTTWKLDIISESSAASRTAESIFNL
HHHCCCCCEEEEEECCCCEEEEECCCCCCEEEEEEEEEEEEEEECCCCHHHHHHHHHHHH
MLIPGMSETMAQNIFQSGFGSFQAVATAAVEELMTIPGYDDPDKAEKLSKEAKGLVAKYE
HHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
SEGVPVPTAPSVAKDKGSNVSAKEQADLLLKQELKKLEAEEE
CCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AENVFSDLSKVIDQVGKDKGIDKQVVIDAITQGMLVAARKKYGTYREIEAAYNEDTGEV
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCE
ELFEFKEVVPREKYIDEEVEIPLDEAQKLDPNVQLDDSIGIKMEASDLGRIAAQTAKQII
EEHHHHHHCCHHHHCCCCCCCCHHHHHCCCCCCEECCCCCCEEEHHHHHHHHHHHHHHHH
MQKVRDAERNIIFNEFEERKGEIASGIARRVEKGAIVVDLGRTEAYIPPREQIPGEQYKP
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHCCCCCCCC
GDRIQGYLSEVRQTTRGPQIIMSRADERYLMKLFEMEVPEIYDGVVEIMAAAREPGQRAK
HHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCE
IAVRSKDNSVDPVGACVGMKGSRVQNIVQELRGEKIDIVPWDEDITRFACNALAPAEISR
EEEECCCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCHHHHHH
VFLDDANREMEIVVPDSQLSLAIGKRGQNVRLAAKLTTWKLDIISESSAASRTAESIFNL
HHHCCCCCEEEEEECCCCEEEEECCCCCCEEEEEEEEEEEEEEECCCCHHHHHHHHHHHH
MLIPGMSETMAQNIFQSGFGSFQAVATAAVEELMTIPGYDDPDKAEKLSKEAKGLVAKYE
HHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
SEGVPVPTAPSVAKDKGSNVSAKEQADLLLKQELKKLEAEEE
CCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]