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The map label for this gene is camA [H]

Identifier: 42522951

GI number: 42522951

Start: 1352131

End: 1353696

Strand: Direct

Name: camA [H]

Synonym: Bd1435

Alternate gene names: 42522951

Gene position: 1352131-1353696 (Clockwise)

Preceding gene: 42522949

Following gene: 42522953

Centisome position: 35.74

GC content: 54.02

Gene sequence:

>1566_bases
ATGGGAACCAGTGCTGAATTGAAAGGACCGAATTTATCCAAGGGAGTGGATCTCAGCGAGCTGCGTGAAGATAAAAGTCT
GCTGGGCTATGTCGGGGAAGAGCCGGTGATTCTGGTTCGCCATGATGAAGAGTACTTTGCCATCGGTGCGACCTGTTCAC
ACTATGGCGGTCCTCTGGCTGATGGACTGGTGGTCGGAGAAACTGTGCACTGTCCATGGCACCATGCCTGTTTTGATCTG
CGCACGGGGGAGGCATTGAAGGCTCCGGCTTTGAATCCTGTTTCGGCCTACAATGTGGAAGTGCGCGGAAGCAAGGTCTT
TGTCACCAGCAAAAAAGAAAGCACGGTGCGGCCGCGGGAGTGGAGTGAATCGCAGAGGTACGTCATCGTGGGTTCCGGAG
CTGCGGGGACCGCCGCGGCAATCATGCTGCGCAAACAGGGTTTTATCGGTTCCATCACTATTGTCAGCGAAGACAAGTCG
TTGCCTTATGACCGTCCGAATCTGTCGAAGGACTATCTGGCGGGGAATATCCCGGAAGACTGGGTGCCTTTGGAGACCGA
GGAATTCTATCAGACTCACAAAATTCACTTTGAACTTTCAACGAAGGCGGAAAAGGTCGATGCTCACAGGCGCAGTGTGT
TCCTGTCGAATGGAAAAACTTTGCGCTATGACCGACTGTTGCTGGCGACAGGTGGGGAACCGATCCACCCGCCGATTCCG
GGCATCAAGCAGGATCACGTGTTTTATCTGCGCACCCTGCAGGACTGCCAGCGCATTATCGGTCGCACATCCTGGGCGCA
AAAAGTGGTGATTGTGGGGGCCGGATTTATCGGCCTTGAGGTGGCGGCAGCCCTTCGCCAGCGCAATCTGGAAGTGCATG
TGGTGGCACCGGAAGAGATGCCCTTGTTGAAAGTTGTCGGCGTCCATGTTGGAAGTGTGCTGCACAAACTGCATGAAGAA
CACGGGGTGATCTTCCATCTGGGGCACACGATCAAAGAAATCCGTCAGCGCAGTGTTCTGCTGGATGACGGGCACAGTGT
GGACTGTGACTTTGTGATAGTGGGCACGGGAATTCACCCTAACACTCAATTGGCTGAACAGGCGGGTTGCTGGGTCGAAA
ATGGGGTGCTGGTGAATGAATATCTTGAAACCAGTGTGCCTGGCATTTTTGCGGCGGGGGACATTGCCAGATGGCCAGAC
CCTCACAGTCAGCGCTCCATCCGGGTGGAACACTGGGAGGTCGCCGAACGCCAAGGGCAAACGGCGGCATTGAACATGAT
GGGGGACCGGATAAAGTTCCAGGATGTGCCGTTCTTCTGGACACAGCACTATGATTTGAGCTTGGGATACGTCGGACACT
CAGATCGCTTTGATAGAATGGATGTGATGGGGGATCTTAACAGCAGAGATTTCGCTGTGGCCTATTATGAAGATCAAAAA
GTGGCTGCAGTACTGACGCTGGGCCGTGATCGGGAAAGCCTTTTGGTCGAAGAGGCGATCGGCCAGTACGATGAGAAGAA
GATCCACGAGATCTTCCATCAACACGAACGTGATCATCATCCCTAA

Upstream 100 bases:

>100_bases
TTTACATTGCAACTGTGTTGCTTTTTAGCATTTTCGTCGAGGTCCTGCTCCAGCCTGAAGCGGAAAATTACTATGATATG
CCCATTCAAAGGAGTGTCTG

Downstream 100 bases:

>100_bases
AGCAGAAACCCCCGGAAAGATCCGGGGGTGTTTGATTGACTAGTGTGCAGTGGCGGACGGGGAACGAAGTTCGTGGAAGT
CGATTTCTAAAGAAATCTCA

Product: putative NAD(FAD)-dependent dehydrogenase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MGTSAELKGPNLSKGVDLSELREDKSLLGYVGEEPVILVRHDEEYFAIGATCSHYGGPLADGLVVGETVHCPWHHACFDL
RTGEALKAPALNPVSAYNVEVRGSKVFVTSKKESTVRPREWSESQRYVIVGSGAAGTAAAIMLRKQGFIGSITIVSEDKS
LPYDRPNLSKDYLAGNIPEDWVPLETEEFYQTHKIHFELSTKAEKVDAHRRSVFLSNGKTLRYDRLLLATGGEPIHPPIP
GIKQDHVFYLRTLQDCQRIIGRTSWAQKVVIVGAGFIGLEVAAALRQRNLEVHVVAPEEMPLLKVVGVHVGSVLHKLHEE
HGVIFHLGHTIKEIRQRSVLLDDGHSVDCDFVIVGTGIHPNTQLAEQAGCWVENGVLVNEYLETSVPGIFAAGDIARWPD
PHSQRSIRVEHWEVAERQGQTAALNMMGDRIKFQDVPFFWTQHYDLSLGYVGHSDRFDRMDVMGDLNSRDFAVAYYEDQK
VAAVLTLGRDRESLLVEEAIGQYDEKKIHEIFHQHERDHHP

Sequences:

>Translated_521_residues
MGTSAELKGPNLSKGVDLSELREDKSLLGYVGEEPVILVRHDEEYFAIGATCSHYGGPLADGLVVGETVHCPWHHACFDL
RTGEALKAPALNPVSAYNVEVRGSKVFVTSKKESTVRPREWSESQRYVIVGSGAAGTAAAIMLRKQGFIGSITIVSEDKS
LPYDRPNLSKDYLAGNIPEDWVPLETEEFYQTHKIHFELSTKAEKVDAHRRSVFLSNGKTLRYDRLLLATGGEPIHPPIP
GIKQDHVFYLRTLQDCQRIIGRTSWAQKVVIVGAGFIGLEVAAALRQRNLEVHVVAPEEMPLLKVVGVHVGSVLHKLHEE
HGVIFHLGHTIKEIRQRSVLLDDGHSVDCDFVIVGTGIHPNTQLAEQAGCWVENGVLVNEYLETSVPGIFAAGDIARWPD
PHSQRSIRVEHWEVAERQGQTAALNMMGDRIKFQDVPFFWTQHYDLSLGYVGHSDRFDRMDVMGDLNSRDFAVAYYEDQK
VAAVLTLGRDRESLLVEEAIGQYDEKKIHEIFHQHERDHHP
>Mature_520_residues
GTSAELKGPNLSKGVDLSELREDKSLLGYVGEEPVILVRHDEEYFAIGATCSHYGGPLADGLVVGETVHCPWHHACFDLR
TGEALKAPALNPVSAYNVEVRGSKVFVTSKKESTVRPREWSESQRYVIVGSGAAGTAAAIMLRKQGFIGSITIVSEDKSL
PYDRPNLSKDYLAGNIPEDWVPLETEEFYQTHKIHFELSTKAEKVDAHRRSVFLSNGKTLRYDRLLLATGGEPIHPPIPG
IKQDHVFYLRTLQDCQRIIGRTSWAQKVVIVGAGFIGLEVAAALRQRNLEVHVVAPEEMPLLKVVGVHVGSVLHKLHEEH
GVIFHLGHTIKEIRQRSVLLDDGHSVDCDFVIVGTGIHPNTQLAEQAGCWVENGVLVNEYLETSVPGIFAAGDIARWPDP
HSQRSIRVEHWEVAERQGQTAALNMMGDRIKFQDVPFFWTQHYDLSLGYVGHSDRFDRMDVMGDLNSRDFAVAYYEDQKV
AAVLTLGRDRESLLVEEAIGQYDEKKIHEIFHQHERDHHP

Specific function: The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI21389617, Length=505, Percent_Identity=33.4653465346535, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI65787454, Length=476, Percent_Identity=33.8235294117647, Blast_Score=288, Evalue=6e-78,
Organism=Homo sapiens, GI226437568, Length=476, Percent_Identity=33.8235294117647, Blast_Score=288, Evalue=6e-78,
Organism=Homo sapiens, GI22202629, Length=329, Percent_Identity=25.8358662613982, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI4757732, Length=329, Percent_Identity=26.4437689969605, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1788892, Length=368, Percent_Identity=30.7065217391304, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1789065, Length=277, Percent_Identity=30.3249097472924, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1789765, Length=283, Percent_Identity=30.3886925795053, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1789915, Length=206, Percent_Identity=30.5825242718447, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1787114, Length=303, Percent_Identity=25.4125412541254, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17559934, Length=478, Percent_Identity=30.5439330543933, Blast_Score=207, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI32564386, Length=348, Percent_Identity=26.7241379310345, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24639257, Length=479, Percent_Identity=34.0292275574113, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI281359715, Length=479, Percent_Identity=34.0292275574113, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI281359713, Length=479, Percent_Identity=34.0292275574113, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24639250, Length=479, Percent_Identity=34.0292275574113, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI18543267, Length=479, Percent_Identity=34.0292275574113, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24639252, Length=479, Percent_Identity=34.0292275574113, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24585130, Length=518, Percent_Identity=31.4671814671815, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI28573993, Length=369, Percent_Identity=28.9972899728997, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24581020, Length=369, Percent_Identity=28.9972899728997, Blast_Score=109, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 58271; Mature: 58139

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTSAELKGPNLSKGVDLSELREDKSLLGYVGEEPVILVRHDEEYFAIGATCSHYGGPLA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCHHHCCCCCC
DGLVVGETVHCPWHHACFDLRTGEALKAPALNPVSAYNVEVRGSKVFVTSKKESTVRPRE
CCEEECCEECCCCHHHEEECCCCCCCCCCCCCCCEEEEEEEECCEEEEECCCCCCCCCCC
WSESQRYVIVGSGAAGTAAAIMLRKQGFIGSITIVSEDKSLPYDRPNLSKDYLAGNIPED
CCCCCCEEEEECCCCCHHHEEEEECCCCEEEEEEEECCCCCCCCCCCCCHHHHCCCCCCC
WVPLETEEFYQTHKIHFELSTKAEKVDAHRRSVFLSNGKTLRYDRLLLATGGEPIHPPIP
CCCCCHHHHHEEEEEEEEECCCHHHHHHHHHEEEECCCCEEEEEEEEEEECCCCCCCCCC
GIKQDHVFYLRTLQDCQRIIGRTSWAQKVVIVGAGFIGLEVAAALRQRNLEVHVVAPEEM
CCCCCCCHHHHHHHHHHHHHCCCCHHCEEEEEECCHHHHHHHHHHHHCCCEEEEECCCCC
PLLKVVGVHVGSVLHKLHEEHGVIFHLGHTIKEIRQRSVLLDDGHSVDCDFVIVGTGIHP
CHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHCCEEEECCCCCCEEEEEEECCCCC
NTQLAEQAGCWVENGVLVNEYLETSVPGIFAAGDIARWPDPHSQRSIRVEHWEVAERQGQ
CCHHHHHCCCEEECCEEEEHHHHHCCCCEEECCCCCCCCCCCCCCCEEEEHHHHHHHCCC
TAALNMMGDRIKFQDVPFFWTQHYDLSLGYVGHSDRFDRMDVMGDLNSRDFAVAYYEDQK
EEEEECCCCEEEEECCCEEEEEEEEEEEEECCCCCCCCHHHHHCCCCCCCEEEEEECCCC
VAAVLTLGRDRESLLVEEAIGQYDEKKIHEIFHQHERDHHP
EEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GTSAELKGPNLSKGVDLSELREDKSLLGYVGEEPVILVRHDEEYFAIGATCSHYGGPLA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCHHHCCCCCC
DGLVVGETVHCPWHHACFDLRTGEALKAPALNPVSAYNVEVRGSKVFVTSKKESTVRPRE
CCEEECCEECCCCHHHEEECCCCCCCCCCCCCCCEEEEEEEECCEEEEECCCCCCCCCCC
WSESQRYVIVGSGAAGTAAAIMLRKQGFIGSITIVSEDKSLPYDRPNLSKDYLAGNIPED
CCCCCCEEEEECCCCCHHHEEEEECCCCEEEEEEEECCCCCCCCCCCCCHHHHCCCCCCC
WVPLETEEFYQTHKIHFELSTKAEKVDAHRRSVFLSNGKTLRYDRLLLATGGEPIHPPIP
CCCCCHHHHHEEEEEEEEECCCHHHHHHHHHEEEECCCCEEEEEEEEEEECCCCCCCCCC
GIKQDHVFYLRTLQDCQRIIGRTSWAQKVVIVGAGFIGLEVAAALRQRNLEVHVVAPEEM
CCCCCCCHHHHHHHHHHHHHCCCCHHCEEEEEECCHHHHHHHHHHHHCCCEEEEECCCCC
PLLKVVGVHVGSVLHKLHEEHGVIFHLGHTIKEIRQRSVLLDDGHSVDCDFVIVGTGIHP
CHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHCCEEEECCCCCCEEEEEEECCCCC
NTQLAEQAGCWVENGVLVNEYLETSVPGIFAAGDIARWPDPHSQRSIRVEHWEVAERQGQ
CCHHHHHCCCEEECCEEEEHHHHHCCCCEEECCCCCCCCCCCCCCCEEEEHHHHHHHCCC
TAALNMMGDRIKFQDVPFFWTQHYDLSLGYVGHSDRFDRMDVMGDLNSRDFAVAYYEDQK
EEEEECCCCEEEEECCCEEEEEEEEEEEEECCCCCCCCHHHHHCCCCCCCEEEEEECCCC
VAAVLTLGRDRESLLVEEAIGQYDEKKIHEIFHQHERDHHP
EEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2180940; 2613690; 3003058; 12011076; 11524002 [H]