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The map label for this gene is mdeA [H]

Identifier: 42522905

GI number: 42522905

Start: 1312215

End: 1313495

Strand: Direct

Name: mdeA [H]

Synonym: Bd1386

Alternate gene names: 42522905

Gene position: 1312215-1313495 (Clockwise)

Preceding gene: 42522904

Following gene: 42522907

Centisome position: 34.69

GC content: 50.66

Gene sequence:

>1281_bases
ATGACCAAGAAAAAAGCGGCCAAATCCAAGTCACCAAGAACTCAAGCCATTCACGGCGAGTTTCTTTCCAAATCCTGGGA
GTTCTCTCACCATTTGATTCCGCCGATGACGGCTTCGACGACATTCCGTCTGGAATCCCTGCAAAGAGGTGCGGAAGGTT
TTAGCACTTTCGGCGCACAGAAGGGCGAAGAAAAACCCATCTGGATCTACGACCGTCTGGAAGAGCCGACAACAAAAATG
CTGGAAGACCAACTGGCCCTTCTTGAAAAAGGCGAGTGTGCGGTGACCTTCGGAAGCGGCATGGGCGCAATCGCCTCCAC
TCTGATGTCATTGCTTTCTTCGGGCCAGAAAGTCATTGCCCATAAAACTTTGTACGGGTGCACCTACAGCCTTTTGACGA
ACTGGCTGCCGCGTTTGAATATTTCCACGCATCTGATGGATGTGAATGATTTCAAAAATCTTGAGCTGCAGCTGGCGGAC
CCGGCAGCACGCGTGGTGTACTTTGAATCTGTTTCCAATCCTATTTTGGAAATCGCGGATCTTCAAAAGATCACCGCGCT
GGTGAAAGCTGCCAACAAAAAACGCGGCAAGGACAATCAGATCTATACTGTTGTGGATAACACTTTCGCGACTCCGTGGG
CGCTTCGTCCGGTGGAATGGAACGTGGACTTCGTGATCCAAAGTTTGACGAAAAACATCTCTGGCTTCGGTACGGAAATG
GGCGGGGCCGTGATCGCTCCAAGAACGCATGAAAGCATTCTGCGTGTGGCGCGCAAGGATTTCGGTGCGATTATCCATCC
ATATTCGGCATGGCACATTCTGGTGTACGGTATTTCCACCCAGGCCATTCGTTTTGAACAGCAGCAGGCATCAGCCTGGG
CGGTGGCCAAGTTCCTGGAAGCGCACCCGAAAGTGGCCAGTGTCACTTATCCGGGCCTGAAAAGTCATGCTCAGCACAAG
CTGGCGAAAAAATATCTAAAATCCCCGGAGGGCAAGTTTGCTCCGGGCACGATGATTTCCTTCCAACTTAAAGGTGACAT
GAAAAAGTGTCAGAAGTTTGTCGATGATATCGCCAAGAATTCATACTCGATCACTCTGGCCGTCAGCTTGGGACTGACCA
AAACGTTGATTGAGGTTCCGGGCTTTATGACTCACTCGGCGATCCCGGATGACAAGCGCGGCGACAGCGGTATTGATCCG
CGCGCCATCCGTCTGAGTATCGGTTTGGAGAACGTAAATGACATTATTGAGGATCTGAAAGAAGCCCTCAAAAAAATCTA
A

Upstream 100 bases:

>100_bases
CAGATGTTCCATTTACCGCCGCGTCGTGGGTCTAAGAGTAGAGCTTTCCTTCAATTAGAACCTGTGCTCTAGTGATTCTC
ATACAATTTATGGGGATCCA

Downstream 100 bases:

>100_bases
AATTCGCGTGGAGGACGCTGGGATTCGTCCTTCACATCCAGTGTATAGCTGGTGGCCGTGTCAGAGATCATGTAGCTCAT
CTCGGGCAGGCCCTCAGCGA

Product: Cys/Met metabolism lyase (PLP-dependent)

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTKKKAAKSKSPRTQAIHGEFLSKSWEFSHHLIPPMTASTTFRLESLQRGAEGFSTFGAQKGEEKPIWIYDRLEEPTTKM
LEDQLALLEKGECAVTFGSGMGAIASTLMSLLSSGQKVIAHKTLYGCTYSLLTNWLPRLNISTHLMDVNDFKNLELQLAD
PAARVVYFESVSNPILEIADLQKITALVKAANKKRGKDNQIYTVVDNTFATPWALRPVEWNVDFVIQSLTKNISGFGTEM
GGAVIAPRTHESILRVARKDFGAIIHPYSAWHILVYGISTQAIRFEQQQASAWAVAKFLEAHPKVASVTYPGLKSHAQHK
LAKKYLKSPEGKFAPGTMISFQLKGDMKKCQKFVDDIAKNSYSITLAVSLGLTKTLIEVPGFMTHSAIPDDKRGDSGIDP
RAIRLSIGLENVNDIIEDLKEALKKI

Sequences:

>Translated_426_residues
MTKKKAAKSKSPRTQAIHGEFLSKSWEFSHHLIPPMTASTTFRLESLQRGAEGFSTFGAQKGEEKPIWIYDRLEEPTTKM
LEDQLALLEKGECAVTFGSGMGAIASTLMSLLSSGQKVIAHKTLYGCTYSLLTNWLPRLNISTHLMDVNDFKNLELQLAD
PAARVVYFESVSNPILEIADLQKITALVKAANKKRGKDNQIYTVVDNTFATPWALRPVEWNVDFVIQSLTKNISGFGTEM
GGAVIAPRTHESILRVARKDFGAIIHPYSAWHILVYGISTQAIRFEQQQASAWAVAKFLEAHPKVASVTYPGLKSHAQHK
LAKKYLKSPEGKFAPGTMISFQLKGDMKKCQKFVDDIAKNSYSITLAVSLGLTKTLIEVPGFMTHSAIPDDKRGDSGIDP
RAIRLSIGLENVNDIIEDLKEALKKI
>Mature_425_residues
TKKKAAKSKSPRTQAIHGEFLSKSWEFSHHLIPPMTASTTFRLESLQRGAEGFSTFGAQKGEEKPIWIYDRLEEPTTKML
EDQLALLEKGECAVTFGSGMGAIASTLMSLLSSGQKVIAHKTLYGCTYSLLTNWLPRLNISTHLMDVNDFKNLELQLADP
AARVVYFESVSNPILEIADLQKITALVKAANKKRGKDNQIYTVVDNTFATPWALRPVEWNVDFVIQSLTKNISGFGTEMG
GAVIAPRTHESILRVARKDFGAIIHPYSAWHILVYGISTQAIRFEQQQASAWAVAKFLEAHPKVASVTYPGLKSHAQHKL
AKKYLKSPEGKFAPGTMISFQLKGDMKKCQKFVDDIAKNSYSITLAVSLGLTKTLIEVPGFMTHSAIPDDKRGDSGIDPR
AIRLSIGLENVNDIIEDLKEALKKI

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=412, Percent_Identity=32.0388349514563, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI299473759, Length=412, Percent_Identity=30.0970873786408, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI25453487, Length=412, Percent_Identity=28.6407766990291, Blast_Score=125, Evalue=6e-29,
Organism=Escherichia coli, GI1790375, Length=395, Percent_Identity=28.3544303797468, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1789383, Length=365, Percent_Identity=26.5753424657534, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17538055, Length=416, Percent_Identity=32.6923076923077, Blast_Score=194, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI17560056, Length=413, Percent_Identity=32.2033898305085, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI71980708, Length=399, Percent_Identity=30.8270676691729, Blast_Score=179, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6319307, Length=418, Percent_Identity=32.5358851674641, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=29.4930875576037, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6321254, Length=415, Percent_Identity=27.710843373494, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6321170, Length=308, Percent_Identity=25, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19922554, Length=412, Percent_Identity=33.7378640776699, Blast_Score=205, Evalue=6e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 47164; Mature: 47033

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKKAAKSKSPRTQAIHGEFLSKSWEFSHHLIPPMTASTTFRLESLQRGAEGFSTFGAQ
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
KGEEKPIWIYDRLEEPTTKMLEDQLALLEKGECAVTFGSGMGAIASTLMSLLSSGQKVIA
CCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHH
HKTLYGCTYSLLTNWLPRLNISTHLMDVNDFKNLELQLADPAARVVYFESVSNPILEIAD
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCEEEEEEHHCCCCHHHHHH
LQKITALVKAANKKRGKDNQIYTVVDNTFATPWALRPVEWNVDFVIQSLTKNISGFGTEM
HHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCEECCHHHHHHHHHHHHHCCCCCC
GGAVIAPRTHESILRVARKDFGAIIHPYSAWHILVYGISTQAIRFEQQQASAWAVAKFLE
CCEEECCCCHHHHHHHHHHHHCCEECCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHH
AHPKVASVTYPGLKSHAQHKLAKKYLKSPEGKFAPGTMISFQLKGDMKKCQKFVDDIAKN
CCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCC
SYSITLAVSLGLTKTLIEVPGFMTHSAIPDDKRGDSGIDPRAIRLSIGLENVNDIIEDLK
CEEEEEEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHH
EALKKI
HHHHCC
>Mature Secondary Structure 
TKKKAAKSKSPRTQAIHGEFLSKSWEFSHHLIPPMTASTTFRLESLQRGAEGFSTFGAQ
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
KGEEKPIWIYDRLEEPTTKMLEDQLALLEKGECAVTFGSGMGAIASTLMSLLSSGQKVIA
CCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHH
HKTLYGCTYSLLTNWLPRLNISTHLMDVNDFKNLELQLADPAARVVYFESVSNPILEIAD
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCEEEEEEHHCCCCHHHHHH
LQKITALVKAANKKRGKDNQIYTVVDNTFATPWALRPVEWNVDFVIQSLTKNISGFGTEM
HHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCEECCHHHHHHHHHHHHHCCCCCC
GGAVIAPRTHESILRVARKDFGAIIHPYSAWHILVYGISTQAIRFEQQQASAWAVAKFLE
CCEEECCCCHHHHHHHHHHHHCCEECCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHH
AHPKVASVTYPGLKSHAQHKLAKKYLKSPEGKFAPGTMISFQLKGDMKKCQKFVDDIAKN
CCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCC
SYSITLAVSLGLTKTLIEVPGFMTHSAIPDDKRGDSGIDPRAIRLSIGLENVNDIIEDLK
CEEEEEEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHH
EALKKI
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]