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The map label for this gene is nrdE

Identifier: 42522732

GI number: 42522732

Start: 1136102

End: 1138396

Strand: Direct

Name: nrdE

Synonym: Bd1196

Alternate gene names: 42522732

Gene position: 1136102-1138396 (Clockwise)

Preceding gene: 42522728

Following gene: 42522733

Centisome position: 30.03

GC content: 54.29

Gene sequence:

>2295_bases
ATGAAAAAACAAACTCTGCACAGCCCGTCGTACTTTGTTTCTGGAGGCAAAAATCCAGAGAGTCTTTTCGCCTGGAAAAA
TGTGAATTGCGAAATTCGCAACCGCAAGGGCGAAGTGTTTTTCGAAATGAAGAATGTGGAGGCACCCGAGGGCTGGTCCC
AATTGGCAATTGATATTGCCGCCAGCAAGTACTTTAGAAAAGTCGGTGTTCCCAAGACCGGGCATGAGACTTCGGTTCGT
CAGATGGTGGACCGGGTGGTGAAGGCGATTGTGGCCTCTGCGATTCGCCAGGGGGGATACTTTGCCACGCGCAAAGAGGC
CGACATCTTTGCCAAAGAATTGAAGTTCATTCTGCTGTCCCAGCGGGGCGCGTTCAACAGCCCGGTGTGGTTCAATGCCG
GTTTGTGGGAGGCTTACAAAGCACAAAGCCCCAGTGAACATTTCGCCTGGGACGAAAAAAAGAAATCCATTCAGGCCACC
AAAAATGCCTATGAGCGGCCGCAGTGTTCAGCGTGTTTCATTCAAAGTGTCGATGACAGTATCGAGAGCATCTTTGATCT
GGCCAAAACTGAAGCCAAGCTTTTCAAATATGGTTCGGGCACGGGCAGCAACTTCTCAAAAATTCGCAGCAAGTACGAGG
CCACCGGATCGGGCGGAAAAAGTTCGGGCCTGATTTCCTTTTTGGAGGTTCTGGACAAGGGCGCGGGTGCGATCAAATCC
GGCGGGACCACCCGCCGGGCCGCCAAGATGGTGGTGGTGGACATAGATCACCCCGAGGTTCTGGATTTCATTGACTGGAA
AATGCGCGAAGAGCAAAAGGCGCATCTGCTGATTGCCGCAGGACTCAGTGCGGATTTCGAGGGCGAGGCCTATCGCACGG
TATCCGGGCAGAACGCCAACAACTCGGTCCGGGTCAGCGATGCCTTCATGAAAGCGGTGGAACAAGAGCGACCGTGGAAG
CTCAAGGCGCGTTTGACCGGAAAAGTCTTGCGTGAAATTCCAGCCGGCGAAGTCTGGAACCGTATCACACGCGCGGCGTG
GATGTGCGCGGATCCCGGCATTCAGTTCCATGACACCATCAACAAGTGGCACACATGTCCTGAGACAGACATCATTCATT
CCAGCAACCCGTGTTCTGAATACATGTTCCTGGATGATTCCGCCTGCAATCTGGCTTCCATCAACCTGGTGAAGTTCTTA
AACGACACCGGAGACTTTGATTTTGAATCCTTTATTCATACGGCCCGCACGCTGTTTGTGGCGCAGGAAATTCTGGTGGA
CTATTCCAGCTATCCAACCCAAAGAGTTGCGCAGAATTCCCATGACTATCGTCCTCTGGGGTTGGGCTTTGCCAATCTGG
GCAGCCTCCTGATGCGAAAGGGCGTAGCCTATGACAGCGACGAGGGCCGCGCGTGGGCGGGGGCTTTGACATCGTTGATG
TCGGGGGTGGCTTATCTGACCAGTTCCGAGATGGCCCGGGCCAAGGGGCCGTTTGCGGGATATCGCAAAAACAGCAAATC
GATGCTGAAAGTGATGAAGATGCACGAGCGTGCCCTGAATCAGGTGGCGTGGAAGATGCTTCCCGCCGGCATAGACAAGG
CCGTCCGGAATTTATGGAAGGGCGTCTTGTACAACGGCGAAAAGTACGGCTATCGAAACGCTCAAGCCACGGTGATCGCA
CCGACCGGGACTATCGGACTTTTGATGGATTGTGACACCACCGGGATTGAGCCGGACTTTTCTTTGATCAAGTTCAAGAA
ACTGGTCGGCGGTGGCGAAATTCAGATTGTGAACCAGTCCGTGGACGCGGCTTTGGCGTCCCTGCAATACTTCGAAGACG
AACGTCAGGCGATTTTGAAATATGTCGAAGAAAACAACTCGGTCGTGGGCGCTCCGCAAATGCACCCGGAAGACCTGCCG
GTCTTTGACACCGCCACGGCAATGCCAGGCCAGCGGGTTTTGTCCCCGGAAAGTCATGTGAAGATGATGGCGGCAGTGCA
GCCGTTTATCAGCGGGGCCATCTCCAAAACCGTGAACATGCCAAGCACGGCCACCGAAGAGGACATCAGCCGCATTTACT
TCCTGGCCTGGAAGCTGGGGGTGAAGGCCGTGGCGGTGTACCGCGATGGCAGCAAACAAAGCCAGCCTCTGAACCTGAAG
GAAAAGCCCAAAGTGGTGGAGGAAGTCGGTGTTCCGAATTTCACTATGAAATGCCCGGAATGCGGGAGTGACACAGTGCT
TACGAGCGGCTGTTACAGATGTCCAAACTGCGGCACGACGGTGGGTTGTTCCTAA

Upstream 100 bases:

>100_bases
CTTAACAGAACACAGGCCCTTGGGCCATGCTTGTATCGCACATCTGTACTGTCCAAATCGTGGGCTTGGCTCAGAAGGAA
AGAATCTCTAGACTGAATGT

Downstream 100 bases:

>100_bases
AGACTTGAAGGGCAACTCGCTGCGGCGGTGCTGGTCAAAGTTTGGTGGGACCTTTATCCTCTCTGTTCTCTTTAACTAAG
GACTGCTTTATGTCTAAAAA

Product: ribonucleotide-diphosphate reductase alpha subunit

Products: NA

Alternate protein names: Ribonucleoside-diphosphate reductase nrdJ

Number of amino acids: Translated: 764; Mature: 764

Protein sequence:

>764_residues
MKKQTLHSPSYFVSGGKNPESLFAWKNVNCEIRNRKGEVFFEMKNVEAPEGWSQLAIDIAASKYFRKVGVPKTGHETSVR
QMVDRVVKAIVASAIRQGGYFATRKEADIFAKELKFILLSQRGAFNSPVWFNAGLWEAYKAQSPSEHFAWDEKKKSIQAT
KNAYERPQCSACFIQSVDDSIESIFDLAKTEAKLFKYGSGTGSNFSKIRSKYEATGSGGKSSGLISFLEVLDKGAGAIKS
GGTTRRAAKMVVVDIDHPEVLDFIDWKMREEQKAHLLIAAGLSADFEGEAYRTVSGQNANNSVRVSDAFMKAVEQERPWK
LKARLTGKVLREIPAGEVWNRITRAAWMCADPGIQFHDTINKWHTCPETDIIHSSNPCSEYMFLDDSACNLASINLVKFL
NDTGDFDFESFIHTARTLFVAQEILVDYSSYPTQRVAQNSHDYRPLGLGFANLGSLLMRKGVAYDSDEGRAWAGALTSLM
SGVAYLTSSEMARAKGPFAGYRKNSKSMLKVMKMHERALNQVAWKMLPAGIDKAVRNLWKGVLYNGEKYGYRNAQATVIA
PTGTIGLLMDCDTTGIEPDFSLIKFKKLVGGGEIQIVNQSVDAALASLQYFEDERQAILKYVEENNSVVGAPQMHPEDLP
VFDTATAMPGQRVLSPESHVKMMAAVQPFISGAISKTVNMPSTATEEDISRIYFLAWKLGVKAVAVYRDGSKQSQPLNLK
EKPKVVEEVGVPNFTMKCPECGSDTVLTSGCYRCPNCGTTVGCS

Sequences:

>Translated_764_residues
MKKQTLHSPSYFVSGGKNPESLFAWKNVNCEIRNRKGEVFFEMKNVEAPEGWSQLAIDIAASKYFRKVGVPKTGHETSVR
QMVDRVVKAIVASAIRQGGYFATRKEADIFAKELKFILLSQRGAFNSPVWFNAGLWEAYKAQSPSEHFAWDEKKKSIQAT
KNAYERPQCSACFIQSVDDSIESIFDLAKTEAKLFKYGSGTGSNFSKIRSKYEATGSGGKSSGLISFLEVLDKGAGAIKS
GGTTRRAAKMVVVDIDHPEVLDFIDWKMREEQKAHLLIAAGLSADFEGEAYRTVSGQNANNSVRVSDAFMKAVEQERPWK
LKARLTGKVLREIPAGEVWNRITRAAWMCADPGIQFHDTINKWHTCPETDIIHSSNPCSEYMFLDDSACNLASINLVKFL
NDTGDFDFESFIHTARTLFVAQEILVDYSSYPTQRVAQNSHDYRPLGLGFANLGSLLMRKGVAYDSDEGRAWAGALTSLM
SGVAYLTSSEMARAKGPFAGYRKNSKSMLKVMKMHERALNQVAWKMLPAGIDKAVRNLWKGVLYNGEKYGYRNAQATVIA
PTGTIGLLMDCDTTGIEPDFSLIKFKKLVGGGEIQIVNQSVDAALASLQYFEDERQAILKYVEENNSVVGAPQMHPEDLP
VFDTATAMPGQRVLSPESHVKMMAAVQPFISGAISKTVNMPSTATEEDISRIYFLAWKLGVKAVAVYRDGSKQSQPLNLK
EKPKVVEEVGVPNFTMKCPECGSDTVLTSGCYRCPNCGTTVGCS
>Mature_764_residues
MKKQTLHSPSYFVSGGKNPESLFAWKNVNCEIRNRKGEVFFEMKNVEAPEGWSQLAIDIAASKYFRKVGVPKTGHETSVR
QMVDRVVKAIVASAIRQGGYFATRKEADIFAKELKFILLSQRGAFNSPVWFNAGLWEAYKAQSPSEHFAWDEKKKSIQAT
KNAYERPQCSACFIQSVDDSIESIFDLAKTEAKLFKYGSGTGSNFSKIRSKYEATGSGGKSSGLISFLEVLDKGAGAIKS
GGTTRRAAKMVVVDIDHPEVLDFIDWKMREEQKAHLLIAAGLSADFEGEAYRTVSGQNANNSVRVSDAFMKAVEQERPWK
LKARLTGKVLREIPAGEVWNRITRAAWMCADPGIQFHDTINKWHTCPETDIIHSSNPCSEYMFLDDSACNLASINLVKFL
NDTGDFDFESFIHTARTLFVAQEILVDYSSYPTQRVAQNSHDYRPLGLGFANLGSLLMRKGVAYDSDEGRAWAGALTSLM
SGVAYLTSSEMARAKGPFAGYRKNSKSMLKVMKMHERALNQVAWKMLPAGIDKAVRNLWKGVLYNGEKYGYRNAQATVIA
PTGTIGLLMDCDTTGIEPDFSLIKFKKLVGGGEIQIVNQSVDAALASLQYFEDERQAILKYVEENNSVVGAPQMHPEDLP
VFDTATAMPGQRVLSPESHVKMMAAVQPFISGAISKTVNMPSTATEEDISRIYFLAWKLGVKAVAVYRDGSKQSQPLNLK
EKPKVVEEVGVPNFTMKCPECGSDTVLTSGCYRCPNCGTTVGCS

Specific function: Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase class-2 family

Homologues:

Organism=Homo sapiens, GI4506749, Length=587, Percent_Identity=24.3611584327087, Blast_Score=144, Evalue=3e-34,
Organism=Escherichia coli, GI1789030, Length=587, Percent_Identity=21.8057921635434, Blast_Score=100, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17555242, Length=577, Percent_Identity=24.7833622183709, Blast_Score=140, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6322123, Length=582, Percent_Identity=24.7422680412371, Blast_Score=137, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6320914, Length=599, Percent_Identity=23.5392320534224, Blast_Score=136, Evalue=1e-32,
Organism=Drosophila melanogaster, GI17352481, Length=578, Percent_Identity=24.0484429065744, Blast_Score=138, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NRDJ_BDEBA (Q6MNP6)

Other databases:

- EMBL:   BX842649
- RefSeq:   NP_968112.1
- ProteinModelPortal:   Q6MNP6
- GeneID:   2733233
- GenomeReviews:   BX842601_GR
- KEGG:   bba:Bd1196
- NMPDR:   fig|264462.1.peg.1082
- HOGENOM:   HBG414188
- OMA:   MGILDIS
- BioCyc:   BBAC264462:BD1196-MONOMER
- BRENDA:   1.17.4.1
- InterPro:   IPR013344
- InterPro:   IPR000788
- InterPro:   IPR013678
- PANTHER:   PTHR11573
- PRINTS:   PR01183
- TIGRFAMs:   TIGR02504

Pfam domain/function: PF08471 Ribonuc_red_2_N; PF02867 Ribonuc_red_lgC

EC number: =1.17.4.1

Molecular weight: Translated: 84409; Mature: 84409

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: ACT_SITE 172-172 ACT_SITE 378-378 ACT_SITE 389-389 ACT_SITE 757-757 ACT_SITE 763-763

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQTLHSPSYFVSGGKNPESLFAWKNVNCEIRNRKGEVFFEMKNVEAPEGWSQLAIDIA
CCCCCCCCCCEEECCCCCHHHEEEECCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHH
ASKYFRKVGVPKTGHETSVRQMVDRVVKAIVASAIRQGGYFATRKEADIFAKELKFILLS
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHEE
QRGAFNSPVWFNAGLWEAYKAQSPSEHFAWDEKKKSIQATKNAYERPQCSACFIQSVDDS
CCCCCCCCEEECCCHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
IESIFDLAKTEAKLFKYGSGTGSNFSKIRSKYEATGSGGKSSGLISFLEVLDKGAGAIKS
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCC
GGTTRRAAKMVVVDIDHPEVLDFIDWKMREEQKAHLLIAAGLSADFEGEAYRTVSGQNAN
CCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEECCCCCC
NSVRVSDAFMKAVEQERPWKLKARLTGKVLREIPAGEVWNRITRAAWMCADPGIQFHDTI
CCEEHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCCCHHHHH
NKWHTCPETDIIHSSNPCSEYMFLDDSACNLASINLVKFLNDTGDFDFESFIHTARTLFV
HHHCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
AQEILVDYSSYPTQRVAQNSHDYRPLGLGFANLGSLLMRKGVAYDSDEGRAWAGALTSLM
HHHHHHHHCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
SGVAYLTSSEMARAKGPFAGYRKNSKSMLKVMKMHERALNQVAWKMLPAGIDKAVRNLWK
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVLYNGEKYGYRNAQATVIAPTGTIGLLMDCDTTGIEPDFSLIKFKKLVGGGEIQIVNQS
HHHCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCH
VDAALASLQYFEDERQAILKYVEENNSVVGAPQMHPEDLPVFDTATAMPGQRVLSPESHV
HHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
KMMAAVQPFISGAISKTVNMPSTATEEDISRIYFLAWKLGVKAVAVYRDGSKQSQPLNLK
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCC
EKPKVVEEVGVPNFTMKCPECGSDTVLTSGCYRCPNCGTTVGCS
CCCHHHHHCCCCCCEEECCCCCCCCEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKQTLHSPSYFVSGGKNPESLFAWKNVNCEIRNRKGEVFFEMKNVEAPEGWSQLAIDIA
CCCCCCCCCCEEECCCCCHHHEEEECCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHH
ASKYFRKVGVPKTGHETSVRQMVDRVVKAIVASAIRQGGYFATRKEADIFAKELKFILLS
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHEE
QRGAFNSPVWFNAGLWEAYKAQSPSEHFAWDEKKKSIQATKNAYERPQCSACFIQSVDDS
CCCCCCCCEEECCCHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
IESIFDLAKTEAKLFKYGSGTGSNFSKIRSKYEATGSGGKSSGLISFLEVLDKGAGAIKS
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCC
GGTTRRAAKMVVVDIDHPEVLDFIDWKMREEQKAHLLIAAGLSADFEGEAYRTVSGQNAN
CCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEECCCCCC
NSVRVSDAFMKAVEQERPWKLKARLTGKVLREIPAGEVWNRITRAAWMCADPGIQFHDTI
CCEEHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCCCHHHHH
NKWHTCPETDIIHSSNPCSEYMFLDDSACNLASINLVKFLNDTGDFDFESFIHTARTLFV
HHHCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
AQEILVDYSSYPTQRVAQNSHDYRPLGLGFANLGSLLMRKGVAYDSDEGRAWAGALTSLM
HHHHHHHHCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
SGVAYLTSSEMARAKGPFAGYRKNSKSMLKVMKMHERALNQVAWKMLPAGIDKAVRNLWK
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVLYNGEKYGYRNAQATVIAPTGTIGLLMDCDTTGIEPDFSLIKFKKLVGGGEIQIVNQS
HHHCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCH
VDAALASLQYFEDERQAILKYVEENNSVVGAPQMHPEDLPVFDTATAMPGQRVLSPESHV
HHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
KMMAAVQPFISGAISKTVNMPSTATEEDISRIYFLAWKLGVKAVAVYRDGSKQSQPLNLK
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCC
EKPKVVEEVGVPNFTMKCPECGSDTVLTSGCYRCPNCGTTVGCS
CCCHHHHHCCCCCCEEECCCCCCCCEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA