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The map label for this gene is mepA [H]

Identifier: 42522591

GI number: 42522591

Start: 979154

End: 980710

Strand: Direct

Name: mepA [H]

Synonym: Bd1032

Alternate gene names: 42522591

Gene position: 979154-980710 (Clockwise)

Preceding gene: 42522590

Following gene: 42522592

Centisome position: 25.88

GC content: 51.77

Gene sequence:

>1557_bases
ATGAAGACTTTGTTGCGTGCCCTCATCATTTTAAGCTCCCTCTCAATGCTGGCAGCCTGTGCTCCGGACACCCGTGAACA
GGGTTCTGAGGTCGTGACGACCTATGAGCCGGCACAACCCGATGTGATGGATGAAGACGGTTACAAAGTCGTGCGAGGTT
CGACTCGCGTGCAGGACATGAGTGTTAACTTTGACCAGGCCACCAAGGGCATGACCCTGAAGGGTAAAATCGAATTCCTG
CCAGTGCGTGCGAAAGCCGTGCAGTCTGTAGAGCTGGATCTGGCTGGTATTTTAGATCCTTACGGATTCATCGCTCTTAA
AACCTATAAAGCCAAAACCCAGGACAATCAGGATTTGAAAGTGGCGGCGAAAGCCACGTGTTTGAGTGTCGACGGCGGAT
GCCGTTCGTCTTTCATTGATATCTACGTTTACTATCAGGGCATCGTATATCATCACCAGGTGGAATCCCTGCAAGATGAT
GTCAGTCCGGTGAATGAGGAGCCGATTCCGGGTGAAGAGGGTCTGCAAGGCGACGAAGAATCTGAAATCGAAGGTGGCCA
CGATGAAGTCGAGGGCGAGCCAGGTCGTTACGTCGGTGATATCGCCGATGACATCGAAAAAATTCTGGAAGTAAAGAAGC
CGGAAGCTCCCAAGTCCGAGGAAACTCCTAAGGCTGAAGAGCCTAAAAAAGAAGAACCGAAGAAAGAAGACCCTAAAAAA
GAGGAACCAAAGCAGGACGCTCCGAAAAAGGACGAACCGAAGAAGGATCCTCCAAAGGCCGATCCACCAAAGAAGGAAGA
TCCAAAAAAAGATCCTCCTAAAAAAGAGGAGCCTCCAAAGCGTGAGGAGCCTAAAAAGGATCCACCTAAAACCGGTCAAC
CACGTGTGCCTGAGACGGCTCCGGCGGTGAACAAGGTCAGTCAGGCTATTGGGCCAGTGAATGCGGGCCGTTTGCAGAAT
GCGGCGAACATGCTGACTTACGAGCAGGCGCATGCTCCGACAGGCTATGAGATCATTCGTCCGAAAAGAAAAACTCACTT
CGCGACCAATGAGCTGGCTTACATCATTGTGAAAATGGGTCTTTTGACCAAAAAAGAAATCCCGGGTTACGAGCTGGCGG
TGGGCGATTTGTCCCGAGAAGCGGGTGGTAAACTGGGCTCCCACAAATCCCATCAGAACGGACTGGATGCGGACGTGGCC
TTCTTCTTTAACAACAAGTCCTTCCAAGGATACTTCGCGTCCGCAGTGGCGGTGAACAAACCTCACGCCAACTGGATGCT
GGAGCCTCAATGGAAACTCTTTAAAGAGGTCGTGGGCACTCAGCTTATTGACCGTATCTTTATCCACGGTGCTTTGAAGC
AGGCCCTGTGCAGTCACGCCATTGCCAATGGCGAGTTGACCAAGGGTGACAACTCAAGCCTTGCGGCTCAGACGCTGCGC
CGCCTGATCCCGGAAAAAGACCACCACAATCACTTTCACCTGCGTGTGAAGTGTTCCAAAGCTCAGGTTCGTTGCCGTCA
GATGGCAGAGCCAGTCAATACCACCGGCTGTTTCTAA

Upstream 100 bases:

>100_bases
CTTCTTGTCATACCTATCTAAAACTTTGACAGTCCGTCAGTAAGAAGTCCTCTTAAATTGCAGCTCCCGAAAGCACGGTT
CCTGCAATGTTCCCGGCAGG

Downstream 100 bases:

>100_bases
TCCCAATTGCGGCAGCCGTGGGGCTGTGTCAGAATATAGTCATGGCTGATTGGCGTGATCGTAGTGAGATGAATGGGGAT
GTGGTTTTTTTCCGATACGT

Product: hypothetical protein

Products: Split Peptidoglycan Strands [C]

Alternate protein names: D-alanyl-D-alanine-endopeptidase; DD-endopeptidase [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MKTLLRALIILSSLSMLAACAPDTREQGSEVVTTYEPAQPDVMDEDGYKVVRGSTRVQDMSVNFDQATKGMTLKGKIEFL
PVRAKAVQSVELDLAGILDPYGFIALKTYKAKTQDNQDLKVAAKATCLSVDGGCRSSFIDIYVYYQGIVYHHQVESLQDD
VSPVNEEPIPGEEGLQGDEESEIEGGHDEVEGEPGRYVGDIADDIEKILEVKKPEAPKSEETPKAEEPKKEEPKKEDPKK
EEPKQDAPKKDEPKKDPPKADPPKKEDPKKDPPKKEEPPKREEPKKDPPKTGQPRVPETAPAVNKVSQAIGPVNAGRLQN
AANMLTYEQAHAPTGYEIIRPKRKTHFATNELAYIIVKMGLLTKKEIPGYELAVGDLSREAGGKLGSHKSHQNGLDADVA
FFFNNKSFQGYFASAVAVNKPHANWMLEPQWKLFKEVVGTQLIDRIFIHGALKQALCSHAIANGELTKGDNSSLAAQTLR
RLIPEKDHHNHFHLRVKCSKAQVRCRQMAEPVNTTGCF

Sequences:

>Translated_518_residues
MKTLLRALIILSSLSMLAACAPDTREQGSEVVTTYEPAQPDVMDEDGYKVVRGSTRVQDMSVNFDQATKGMTLKGKIEFL
PVRAKAVQSVELDLAGILDPYGFIALKTYKAKTQDNQDLKVAAKATCLSVDGGCRSSFIDIYVYYQGIVYHHQVESLQDD
VSPVNEEPIPGEEGLQGDEESEIEGGHDEVEGEPGRYVGDIADDIEKILEVKKPEAPKSEETPKAEEPKKEEPKKEDPKK
EEPKQDAPKKDEPKKDPPKADPPKKEDPKKDPPKKEEPPKREEPKKDPPKTGQPRVPETAPAVNKVSQAIGPVNAGRLQN
AANMLTYEQAHAPTGYEIIRPKRKTHFATNELAYIIVKMGLLTKKEIPGYELAVGDLSREAGGKLGSHKSHQNGLDADVA
FFFNNKSFQGYFASAVAVNKPHANWMLEPQWKLFKEVVGTQLIDRIFIHGALKQALCSHAIANGELTKGDNSSLAAQTLR
RLIPEKDHHNHFHLRVKCSKAQVRCRQMAEPVNTTGCF
>Mature_518_residues
MKTLLRALIILSSLSMLAACAPDTREQGSEVVTTYEPAQPDVMDEDGYKVVRGSTRVQDMSVNFDQATKGMTLKGKIEFL
PVRAKAVQSVELDLAGILDPYGFIALKTYKAKTQDNQDLKVAAKATCLSVDGGCRSSFIDIYVYYQGIVYHHQVESLQDD
VSPVNEEPIPGEEGLQGDEESEIEGGHDEVEGEPGRYVGDIADDIEKILEVKKPEAPKSEETPKAEEPKKEEPKKEDPKK
EEPKQDAPKKDEPKKDPPKADPPKKEDPKKDPPKKEEPPKREEPKKDPPKTGQPRVPETAPAVNKVSQAIGPVNAGRLQN
AANMLTYEQAHAPTGYEIIRPKRKTHFATNELAYIIVKMGLLTKKEIPGYELAVGDLSREAGGKLGSHKSHQNGLDADVA
FFFNNKSFQGYFASAVAVNKPHANWMLEPQWKLFKEVVGTQLIDRIFIHGALKQALCSHAIANGELTKGDNSSLAAQTLR
RLIPEKDHHNHFHLRVKCSKAQVRCRQMAEPVNTTGCF

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=220, Percent_Identity=25.4545454545455, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 57339; Mature: 57339

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLLRALIILSSLSMLAACAPDTREQGSEVVTTYEPAQPDVMDEDGYKVVRGSTRVQDM
CHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEECCCCCCCCCCCCCEEEECCCEEEEE
SVNFDQATKGMTLKGKIEFLPVRAKAVQSVELDLAGILDPYGFIALKTYKAKTQDNQDLK
ECCHHHHHCCCEEECEEEEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEE
VAAKATCLSVDGGCRSSFIDIYVYYQGIVYHHQVESLQDDVSPVNEEPIPGEEGLQGDEE
EEEEEEEEECCCCCCHHHEEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
SEIEGGHDEVEGEPGRYVGDIADDIEKILEVKKPEAPKSEETPKAEEPKKEEPKKEDPKK
HCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EEPKQDAPKKDEPKKDPPKADPPKKEDPKKDPPKKEEPPKREEPKKDPPKTGQPRVPETA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAVNKVSQAIGPVNAGRLQNAANMLTYEQAHAPTGYEIIRPKRKTHFATNELAYIIVKMG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHCCHHHHHHHHHHHH
LLTKKEIPGYELAVGDLSREAGGKLGSHKSHQNGLDADVAFFFNNKSFQGYFASAVAVNK
HHHHCCCCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEECC
PHANWMLEPQWKLFKEVVGTQLIDRIFIHGALKQALCSHAIANGELTKGDNSSLAAQTLR
CCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
RLIPEKDHHNHFHLRVKCSKAQVRCRQMAEPVNTTGCF
HHCCCCCCCCEEEEEEEECHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKTLLRALIILSSLSMLAACAPDTREQGSEVVTTYEPAQPDVMDEDGYKVVRGSTRVQDM
CHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEECCCCCCCCCCCCCEEEECCCEEEEE
SVNFDQATKGMTLKGKIEFLPVRAKAVQSVELDLAGILDPYGFIALKTYKAKTQDNQDLK
ECCHHHHHCCCEEECEEEEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEE
VAAKATCLSVDGGCRSSFIDIYVYYQGIVYHHQVESLQDDVSPVNEEPIPGEEGLQGDEE
EEEEEEEEECCCCCCHHHEEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
SEIEGGHDEVEGEPGRYVGDIADDIEKILEVKKPEAPKSEETPKAEEPKKEEPKKEDPKK
HCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EEPKQDAPKKDEPKKDPPKADPPKKEDPKKDPPKKEEPPKREEPKKDPPKTGQPRVPETA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAVNKVSQAIGPVNAGRLQNAANMLTYEQAHAPTGYEIIRPKRKTHFATNELAYIIVKMG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHCCHHHHHHHHHHHH
LLTKKEIPGYELAVGDLSREAGGKLGSHKSHQNGLDADVAFFFNNKSFQGYFASAVAVNK
HHHHCCCCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEECC
PHANWMLEPQWKLFKEVVGTQLIDRIFIHGALKQALCSHAIANGELTKGDNSSLAAQTLR
CCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
RLIPEKDHHNHFHLRVKCSKAQVRCRQMAEPVNTTGCF
HHCCCCCCCCEEEEEEEECHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA