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The map label for this gene is rfaE [H]

Identifier: 42522305

GI number: 42522305

Start: 678221

End: 679249

Strand: Direct

Name: rfaE [H]

Synonym: Bd0720

Alternate gene names: 42522305

Gene position: 678221-679249 (Clockwise)

Preceding gene: 42522303

Following gene: 42522306

Centisome position: 17.93

GC content: 52.77

Gene sequence:

>1029_bases
ATGACAACACCCGTAAAGGCCTCCGTGGGTCCTCAGGAAAAAGAACTTCTTCTAAATCAGATTCCTTTCCTAAAAGGCAA
AAAAATCCTGATTATCGGCGATGTGGGCCTGGATGAGTATGTCATGGGCCAGGTTCGCCGCATCAGCCCCGAAGCACCAG
TCCCGGTTCTGGAAGTGGAAGAAGAAGACATGCGCCTGGGGCTTGCTGCCAACGTGGCTCAAAACGTGGCCAGCCTTGGT
GGGGAAGCCATGCTGGTTTCTGTTGTGGGCGATGACACCGGTGCCAACCTGCTGCGTGAACTGTTCGCCAAAAGTGGTGT
CAGCTGGGATTACATGATCGTCGACAAGGCTCGTCCCACCACACGCAAAACCCGCGTGATGGCCAAACACCATCACCTGG
TGCGTGTGGACTATGAATTGCGCAAGTATCTTTCTGCCGAAGCCGAAGCCCGCCTGATTGAGACTGTTGAAAAGAATGTG
GCTAAAGCCGATTGCGTGGTGATCGAGGATTACGCCAAGGGTGTGATTTCCCGCAACGTCGTGGAAAAGGTTGCTGCTAT
TTGCAAAAAGCACGGAAAAAAACTGATGGTTGATCCCCATCGCAACAACCACGGTTCATTCTATCAGGGCGTGGACTTGA
TCAAGCCAAACTATGATGAAGCGGTTGTATTGACGGGCATGGATTTCGATGATCTGCGTGACAACCCGAACAAGGTTGTG
GATGTGGCGCGTGCTCTGCAAAAACTGACAGGTGCCAAAGATGTCGTTCTGACTCGTGGTAAAGACGGCATGACCATCGT
TTCTGGTGATGAGATCACCGAAGTTCCAACCTATGCCCGTAAGGTCTTTGACGTGACGGGTGCGGGGGACACGGTCATTG
CTGCTCTTTCCTTGGGGCTGGTTTCGGGCTTGTCTTTGGTTCATTCCTGCATGCTGGCGAACTATGCCGCCGGTGTGGTG
GTGGGTAAAGTGGGCTGCGTGCCTTGCGAAATCCCGGAACTGAAGGAATACATCCAAACTGCGCACTAA

Upstream 100 bases:

>100_bases
CATGAGGCCCCCGGGGCCGCCAAGTGCGGGGTTTTGGGGGTGACAGGCCGGGCCTTTTTGGGCTACAAGGGAAGCTCAAT
TGCTCCGGGAGAATGAAAAA

Downstream 100 bases:

>100_bases
CGGAGCCTGCCATGCAAAAGACTTATACTGTGAACAGCAAGCTTTTGCCGGCGGGTTTTCCTCGCGAGTTTGATGCCCAT
ATTTACTTTGAAAGTGCCAG

Product: ADP-heptose synthase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MTTPVKASVGPQEKELLLNQIPFLKGKKILIIGDVGLDEYVMGQVRRISPEAPVPVLEVEEEDMRLGLAANVAQNVASLG
GEAMLVSVVGDDTGANLLRELFAKSGVSWDYMIVDKARPTTRKTRVMAKHHHLVRVDYELRKYLSAEAEARLIETVEKNV
AKADCVVIEDYAKGVISRNVVEKVAAICKKHGKKLMVDPHRNNHGSFYQGVDLIKPNYDEAVVLTGMDFDDLRDNPNKVV
DVARALQKLTGAKDVVLTRGKDGMTIVSGDEITEVPTYARKVFDVTGAGDTVIAALSLGLVSGLSLVHSCMLANYAAGVV
VGKVGCVPCEIPELKEYIQTAH

Sequences:

>Translated_342_residues
MTTPVKASVGPQEKELLLNQIPFLKGKKILIIGDVGLDEYVMGQVRRISPEAPVPVLEVEEEDMRLGLAANVAQNVASLG
GEAMLVSVVGDDTGANLLRELFAKSGVSWDYMIVDKARPTTRKTRVMAKHHHLVRVDYELRKYLSAEAEARLIETVEKNV
AKADCVVIEDYAKGVISRNVVEKVAAICKKHGKKLMVDPHRNNHGSFYQGVDLIKPNYDEAVVLTGMDFDDLRDNPNKVV
DVARALQKLTGAKDVVLTRGKDGMTIVSGDEITEVPTYARKVFDVTGAGDTVIAALSLGLVSGLSLVHSCMLANYAAGVV
VGKVGCVPCEIPELKEYIQTAH
>Mature_341_residues
TTPVKASVGPQEKELLLNQIPFLKGKKILIIGDVGLDEYVMGQVRRISPEAPVPVLEVEEEDMRLGLAANVAQNVASLGG
EAMLVSVVGDDTGANLLRELFAKSGVSWDYMIVDKARPTTRKTRVMAKHHHLVRVDYELRKYLSAEAEARLIETVEKNVA
KADCVVIEDYAKGVISRNVVEKVAAICKKHGKKLMVDPHRNNHGSFYQGVDLIKPNYDEAVVLTGMDFDDLRDNPNKVVD
VARALQKLTGAKDVVLTRGKDGMTIVSGDEITEVPTYARKVFDVTGAGDTVIAALSLGLVSGLSLVHSCMLANYAAGVVV
GKVGCVPCEIPELKEYIQTAH

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789432, Length=320, Percent_Identity=34.6875, Blast_Score=171, Evalue=6e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.-.-

Molecular weight: Translated: 37146; Mature: 37014

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPVKASVGPQEKELLLNQIPFLKGKKILIIGDVGLDEYVMGQVRRISPEAPVPVLEVE
CCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCEEEEC
EEDMRLGLAANVAQNVASLGGEAMLVSVVGDDTGANLLRELFAKSGVSWDYMIVDKARPT
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCC
TRKTRVMAKHHHLVRVDYELRKYLSAEAEARLIETVEKNVAKADCVVIEDYAKGVISRNV
HHHHHHHHHCCCEEEECHHHHHHHCHHHHHHHHHHHHHHHHHHCEEEEEHHHHHHHHHHH
VEKVAAICKKHGKKLMVDPHRNNHGSFYQGVDLIKPNYDEAVVLTGMDFDDLRDNPNKVV
HHHHHHHHHHCCCEEEECCCCCCCCCHHCCCCCCCCCCCCEEEEECCCHHHHCCCHHHHH
DVARALQKLTGAKDVVLTRGKDGMTIVSGDEITEVPTYARKVFDVTGAGDTVIAALSLGL
HHHHHHHHHCCCCCEEEECCCCCCEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
VSGLSLVHSCMLANYAAGVVVGKVGCVPCEIPELKEYIQTAH
HHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TTPVKASVGPQEKELLLNQIPFLKGKKILIIGDVGLDEYVMGQVRRISPEAPVPVLEVE
CCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCEEEEC
EEDMRLGLAANVAQNVASLGGEAMLVSVVGDDTGANLLRELFAKSGVSWDYMIVDKARPT
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCC
TRKTRVMAKHHHLVRVDYELRKYLSAEAEARLIETVEKNVAKADCVVIEDYAKGVISRNV
HHHHHHHHHCCCEEEECHHHHHHHCHHHHHHHHHHHHHHHHHHCEEEEEHHHHHHHHHHH
VEKVAAICKKHGKKLMVDPHRNNHGSFYQGVDLIKPNYDEAVVLTGMDFDDLRDNPNKVV
HHHHHHHHHHCCCEEEECCCCCCCCCHHCCCCCCCCCCCCEEEEECCCHHHHCCCHHHHH
DVARALQKLTGAKDVVLTRGKDGMTIVSGDEITEVPTYARKVFDVTGAGDTVIAALSLGL
HHHHHHHHHCCCCCEEEECCCCCCEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
VSGLSLVHSCMLANYAAGVVVGKVGCVPCEIPELKEYIQTAH
HHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA