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The map label for this gene is cheB [H]

Identifier: 42522175

GI number: 42522175

Start: 537923

End: 539485

Strand: Direct

Name: cheB [H]

Synonym: Bd0580

Alternate gene names: 42522175

Gene position: 537923-539485 (Clockwise)

Preceding gene: 42522174

Following gene: 42522176

Centisome position: 14.22

GC content: 55.09

Gene sequence:

>1563_bases
TTGATGAACACTCATTATCTTTTTCCCGGTAAGCTGGCGGCCTTCAAAGAAGAGACCGTGATTTCCACTTTGCTGGGCTC
TTGCGTGGCGGTGGCTTTGCATGACCCCACAACCCGCATCGGCGGCTTGAATCACTATCTGCTTGCCGATGGCATGCCGG
ACGAACGCGAGAACACGCGCTATGGGGCGCATGCCATTCCGTTGCTGATTGAAGAGTGTGTTCATCTGGGAGCCAATCGC
GGCCGCCTGCAGGCCAAGATTTACGGCGGCGGGAATGTGATCAGTGTTTCCCAGATTGGGGAAGGCATTGGCAAGCGCAA
CATCGAGTTCGCGGAAAAGATCCTGCGTGATCACGGCATTCCGATCCTGGAAAGAAATGTCGCCGGTGAATCGGCGCGCA
CAATTAAAATGAACACGGCCACTTTTGAAGTGCTGCACAACTCCTCGCAAGGGGGCAGTGTTGATAAACCTGTGGATGTC
TCGGGCTTCAGACCCTTGGCTGTGGCCAAAAGCGTGAAGGTTCTGGTCGTCGATGACTCGGCAACCGTGCGAACTCTGTT
CACGAATATCTTCACCAAAAGCGGACTGGAAGTTGTCGGTTCTGCGGCGGACGCCTATCAGGCGCGTGAAATGATTTTGA
GCAAGAAACCCGACGTTCTGACTCTGGACATCGAGATGCCGAAGATGTCGGGTGTGATGTTCCTGGAAAAGCTGATGAAG
CATCACCCGATCCCGGTGGTGATGGTTTCCTCTTTGGCCAGTACCGGTGAAGCGGCGATGAAATCCCTGGAGCTGGGTGC
TGTGGAGTTCGTGCACAAGCCTTCGCAGTTTGACCCGGCGGTTTTAAAGGACCTGGCGGGAATGCTGGTGGACAAAGTCC
GCGCGGCGGCTTCGGTGAATATTTTGAAGAAACTTAAAGAGACTCCGGCCCCTCAGGAAATCAAGACCACCACCTTTGCG
ACTCCGACCCGTCGTCGTGCGGCTGAGTTGAAAGTGGTGGTGCTGGGCGGAAATGCCGGCAGTGCGGAAGCGCTGGAAAA
GTTTGTCAAAGGCCTTGCGGCGGACACGCCTCCGGTGGTGGTGGCCTGCAGTACGGTGGCGAACTTTGTGACGGCTTTCA
TCAGCAAGCTGAAGGTGGGATCCAAAGTCACGCCGGTGGTGGGTAAAGACGGTGATTTCCTCAGAATGGGGCATGTTTAT
TTTGTGCCAGCCGAACACCATGGGAAAATCGTCACCGGTCCTCAGGGACCCGTGCTGAATCTTTCCAAAGGAGCTCCGGT
TTCCTCACAATTGCCTTCGGCGAACGTGCTTTTCAACTCGGCGGCGCAGTCTTATGGGCAGGGTGTTTATGCCCTGCTGT
TGGGTGGGTTTGGCACCGACGGAGTCGATGGACTGGTGGAAGTGCAAAAACGCGGTGGCGCCAGCGTGGTGCAGCACCCG
GAAGAAGCTCAATTTCCCTATGCTCCTCAAAAAGCGATTGAACTGGGAGTGGCGGATGAGATACTTAAAGCCGACATGTT
GGCGCATCACCTGATGCAGTATCGAAACCAAAACTTATACTAG

Upstream 100 bases:

>100_bases
TTGAAAACCAATCCTCCATGATGTCTGCGAACTTTGTTCACCAGTTTTTCATCAAAGACCGCCGCAGTATCATGGTTCTG
GATCTTGAAAAACTGGTGGC

Downstream 100 bases:

>100_bases
GATCTTCGTTGGAACTGCAAAATTTTATCGGAGGGGCCTTTGTCCCTTCCGACAGTCAGAAAACCTTCGCAAAAATTTCT
CCTTTTGATGGCACGGAACT

Product: cheB, glutamate methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MMNTHYLFPGKLAAFKEETVISTLLGSCVAVALHDPTTRIGGLNHYLLADGMPDERENTRYGAHAIPLLIEECVHLGANR
GRLQAKIYGGGNVISVSQIGEGIGKRNIEFAEKILRDHGIPILERNVAGESARTIKMNTATFEVLHNSSQGGSVDKPVDV
SGFRPLAVAKSVKVLVVDDSATVRTLFTNIFTKSGLEVVGSAADAYQAREMILSKKPDVLTLDIEMPKMSGVMFLEKLMK
HHPIPVVMVSSLASTGEAAMKSLELGAVEFVHKPSQFDPAVLKDLAGMLVDKVRAAASVNILKKLKETPAPQEIKTTTFA
TPTRRRAAELKVVVLGGNAGSAEALEKFVKGLAADTPPVVVACSTVANFVTAFISKLKVGSKVTPVVGKDGDFLRMGHVY
FVPAEHHGKIVTGPQGPVLNLSKGAPVSSQLPSANVLFNSAAQSYGQGVYALLLGGFGTDGVDGLVEVQKRGGASVVQHP
EEAQFPYAPQKAIELGVADEILKADMLAHHLMQYRNQNLY

Sequences:

>Translated_520_residues
MMNTHYLFPGKLAAFKEETVISTLLGSCVAVALHDPTTRIGGLNHYLLADGMPDERENTRYGAHAIPLLIEECVHLGANR
GRLQAKIYGGGNVISVSQIGEGIGKRNIEFAEKILRDHGIPILERNVAGESARTIKMNTATFEVLHNSSQGGSVDKPVDV
SGFRPLAVAKSVKVLVVDDSATVRTLFTNIFTKSGLEVVGSAADAYQAREMILSKKPDVLTLDIEMPKMSGVMFLEKLMK
HHPIPVVMVSSLASTGEAAMKSLELGAVEFVHKPSQFDPAVLKDLAGMLVDKVRAAASVNILKKLKETPAPQEIKTTTFA
TPTRRRAAELKVVVLGGNAGSAEALEKFVKGLAADTPPVVVACSTVANFVTAFISKLKVGSKVTPVVGKDGDFLRMGHVY
FVPAEHHGKIVTGPQGPVLNLSKGAPVSSQLPSANVLFNSAAQSYGQGVYALLLGGFGTDGVDGLVEVQKRGGASVVQHP
EEAQFPYAPQKAIELGVADEILKADMLAHHLMQYRNQNLY
>Mature_520_residues
MMNTHYLFPGKLAAFKEETVISTLLGSCVAVALHDPTTRIGGLNHYLLADGMPDERENTRYGAHAIPLLIEECVHLGANR
GRLQAKIYGGGNVISVSQIGEGIGKRNIEFAEKILRDHGIPILERNVAGESARTIKMNTATFEVLHNSSQGGSVDKPVDV
SGFRPLAVAKSVKVLVVDDSATVRTLFTNIFTKSGLEVVGSAADAYQAREMILSKKPDVLTLDIEMPKMSGVMFLEKLMK
HHPIPVVMVSSLASTGEAAMKSLELGAVEFVHKPSQFDPAVLKDLAGMLVDKVRAAASVNILKKLKETPAPQEIKTTTFA
TPTRRRAAELKVVVLGGNAGSAEALEKFVKGLAADTPPVVVACSTVANFVTAFISKLKVGSKVTPVVGKDGDFLRMGHVY
FVPAEHHGKIVTGPQGPVLNLSKGAPVSSQLPSANVLFNSAAQSYGQGVYALLLGGFGTDGVDGLVEVQKRGGASVVQHP
EEAQFPYAPQKAIELGVADEILKADMLAHHLMQYRNQNLY

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR [H]

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=343, Percent_Identity=31.1953352769679, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1790299, Length=108, Percent_Identity=32.4074074074074, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789 [H]

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg [H]

EC number: =3.1.1.61 [H]

Molecular weight: Translated: 55513; Mature: 55513

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50122 CHEB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNTHYLFPGKLAAFKEETVISTLLGSCVAVALHDPTTRIGGLNHYLLADGMPDERENTR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCHHHCCCCEEEEECCCCCCCCCCC
YGAHAIPLLIEECVHLGANRGRLQAKIYGGGNVISVSQIGEGIGKRNIEFAEKILRDHGI
CCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEHHHHHCCCCCCCHHHHHHHHHHCCC
PILERNVAGESARTIKMNTATFEVLHNSSQGGSVDKPVDVSGFRPLAVAKSVKVLVVDDS
CEEEECCCCCCCEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCHHEECCCEEEEEEECC
ATVRTLFTNIFTKSGLEVVGSAADAYQAREMILSKKPDVLTLDIEMPKMSGVMFLEKLMK
HHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHH
HHPIPVVMVSSLASTGEAAMKSLELGAVEFVHKPSQFDPAVLKDLAGMLVDKVRAAASVN
HCCCCEEEHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ILKKLKETPAPQEIKTTTFATPTRRRAAELKVVVLGGNAGSAEALEKFVKGLAADTPPVV
HHHHHHHCCCCCCCCCCCCCCCCHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEE
VACSTVANFVTAFISKLKVGSKVTPVVGKDGDFLRMGHVYFVPAEHHGKIVTGPQGPVLN
EEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCEEEEECCCCCEEEECCCCCEEE
LSKGAPVSSQLPSANVLFNSAAQSYGQGVYALLLGGFGTDGVDGLVEVQKRGGASVVQHP
CCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCHHHCCC
EEAQFPYAPQKAIELGVADEILKADMLAHHLMQYRNQNLY
CCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMNTHYLFPGKLAAFKEETVISTLLGSCVAVALHDPTTRIGGLNHYLLADGMPDERENTR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCHHHCCCCEEEEECCCCCCCCCCC
YGAHAIPLLIEECVHLGANRGRLQAKIYGGGNVISVSQIGEGIGKRNIEFAEKILRDHGI
CCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEHHHHHCCCCCCCHHHHHHHHHHCCC
PILERNVAGESARTIKMNTATFEVLHNSSQGGSVDKPVDVSGFRPLAVAKSVKVLVVDDS
CEEEECCCCCCCEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCHHEECCCEEEEEEECC
ATVRTLFTNIFTKSGLEVVGSAADAYQAREMILSKKPDVLTLDIEMPKMSGVMFLEKLMK
HHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHH
HHPIPVVMVSSLASTGEAAMKSLELGAVEFVHKPSQFDPAVLKDLAGMLVDKVRAAASVN
HCCCCEEEHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ILKKLKETPAPQEIKTTTFATPTRRRAAELKVVVLGGNAGSAEALEKFVKGLAADTPPVV
HHHHHHHCCCCCCCCCCCCCCCCHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEE
VACSTVANFVTAFISKLKVGSKVTPVVGKDGDFLRMGHVYFVPAEHHGKIVTGPQGPVLN
EEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCEEEEECCCCCEEEECCCCCEEE
LSKGAPVSSQLPSANVLFNSAAQSYGQGVYALLLGGFGTDGVDGLVEVQKRGGASVVQHP
CCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCHHHCCC
EEAQFPYAPQKAIELGVADEILKADMLAHHLMQYRNQNLY
CCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA