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The map label for this gene is citZ [H]

Identifier: 42522160

GI number: 42522160

Start: 520456

End: 521595

Strand: Direct

Name: citZ [H]

Synonym: Bd0562

Alternate gene names: 42522160

Gene position: 520456-521595 (Clockwise)

Preceding gene: 42522159

Following gene: 42522163

Centisome position: 13.76

GC content: 49.3

Gene sequence:

>1140_bases
ATGGCTGAAGTAAATATCTATGAAGGCGCCTTGGACAAGGGTTTGGAAGGCGTTGTTGCATGTACGACGAAAGTGTCTTT
TATCGTCGGTGATTCTCTAAATTTCCGTGGTTACACGATCGATGATCTTGCTGCGAATTCCACTTTTGAGGAAGTAACTT
ATCTTCTTTGGAACGACAAACTTCCAAATGCTCAAGAGCTGGCGGCATTCTCCACTCAACTTCGCAATGAAATGGCTTTG
AGCCCTGATTTCATCAAAGTTTTGAAAGCAATCCCTACCAACGTTCACCCAATGGGTTGGTTGCGTACGGCTGTTTCTTT
GATGGCTCACTGGGATTCTGATGCCAACGACAACGGCGCTGAAGCAAACTTGCGCAAGTCTGTTCGTTTGACTGCGAAAA
TGGGAACTCTTCTTTGTGCATTCGATGCCATCCGCAAAGGCAAAGAGCCTGTAGCTCCTAAAGCTGACAAATCCATGGCT
TGGAACATGATGTACATGCTGGGCGGCGGAGCTGAGCCAAAAGCTGAGCACGTAAAAGTGATGGACACGTGCTTGATCCT
TCACGCTGACCACGAGCTGAACTGCTCTGCGTTCGCAACTCGCGTAACCGCTTCTTCTTTGTCTGACCTTCACTCTGCAA
TCGTTTCTGCTATTGGCGCGTTGAAAGGTCCTTTGCACGGTGGTGCGAACGAACAAGTGATCCTGATGCTGCAAAAAATC
GGCACGATGGAAAAAGCTCAGCAGTTCGTTAAAGACGCTTTGGCTGCAAAAGAAAAAGTTATGGGTATCGGTCACCGCGT
TTACAAAAACGGCGACCCTCGCGCTCGTATCCTGCGTGGCATGTCCGACAAGCTGACTAAAGATGCTGGCATCCACCACA
TGTATGAAATGTCCACTTTGATCGACGACACCATGTTCAACGAAAAAGGCTTGATGCCGAACGTGGACTTCTACTCTGCA
ACTGTTTACTTCTCTATGGGCATCCCGACAGATCTGTTCACGCCGATCTTCGCGGCTTCCCGTATTTCCGGCTGGTGCGC
ACACGCCTTCGAGCAGTACGCAAACAACCGTATCTACCGTCCTCGTGGCAAATGGGCAGGCAAAGAGGGTCTGAAATGGA
CTCCAGCCAACCAACGCTAA

Upstream 100 bases:

>100_bases
AGACCTAGGATCGGGAACCGCGTGACAACTTGTGAAAACTCCTGTTAAGTCTACAATGTTTAAAAAAAGAAGTATTTGAT
AAGTCAGAAAGGAAACTACG

Downstream 100 bases:

>100_bases
GAATTTCGGTTTAAGATCGAATGAAAAAGGGAGCTGCAAAGCTCCCTTTTTTATTTCAGAGGCTCGTCTGAAATAAGGGC
CACGTTTTTCTGAATCCAGG

Product: hypothetical protein

Products: NA

Alternate protein names: Citrate synthase II [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MAEVNIYEGALDKGLEGVVACTTKVSFIVGDSLNFRGYTIDDLAANSTFEEVTYLLWNDKLPNAQELAAFSTQLRNEMAL
SPDFIKVLKAIPTNVHPMGWLRTAVSLMAHWDSDANDNGAEANLRKSVRLTAKMGTLLCAFDAIRKGKEPVAPKADKSMA
WNMMYMLGGGAEPKAEHVKVMDTCLILHADHELNCSAFATRVTASSLSDLHSAIVSAIGALKGPLHGGANEQVILMLQKI
GTMEKAQQFVKDALAAKEKVMGIGHRVYKNGDPRARILRGMSDKLTKDAGIHHMYEMSTLIDDTMFNEKGLMPNVDFYSA
TVYFSMGIPTDLFTPIFAASRISGWCAHAFEQYANNRIYRPRGKWAGKEGLKWTPANQR

Sequences:

>Translated_379_residues
MAEVNIYEGALDKGLEGVVACTTKVSFIVGDSLNFRGYTIDDLAANSTFEEVTYLLWNDKLPNAQELAAFSTQLRNEMAL
SPDFIKVLKAIPTNVHPMGWLRTAVSLMAHWDSDANDNGAEANLRKSVRLTAKMGTLLCAFDAIRKGKEPVAPKADKSMA
WNMMYMLGGGAEPKAEHVKVMDTCLILHADHELNCSAFATRVTASSLSDLHSAIVSAIGALKGPLHGGANEQVILMLQKI
GTMEKAQQFVKDALAAKEKVMGIGHRVYKNGDPRARILRGMSDKLTKDAGIHHMYEMSTLIDDTMFNEKGLMPNVDFYSA
TVYFSMGIPTDLFTPIFAASRISGWCAHAFEQYANNRIYRPRGKWAGKEGLKWTPANQR
>Mature_378_residues
AEVNIYEGALDKGLEGVVACTTKVSFIVGDSLNFRGYTIDDLAANSTFEEVTYLLWNDKLPNAQELAAFSTQLRNEMALS
PDFIKVLKAIPTNVHPMGWLRTAVSLMAHWDSDANDNGAEANLRKSVRLTAKMGTLLCAFDAIRKGKEPVAPKADKSMAW
NMMYMLGGGAEPKAEHVKVMDTCLILHADHELNCSAFATRVTASSLSDLHSAIVSAIGALKGPLHGGANEQVILMLQKIG
TMEKAQQFVKDALAAKEKVMGIGHRVYKNGDPRARILRGMSDKLTKDAGIHHMYEMSTLIDDTMFNEKGLMPNVDFYSAT
VYFSMGIPTDLFTPIFAASRISGWCAHAFEQYANNRIYRPRGKWAGKEGLKWTPANQR

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=372, Percent_Identity=25, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1786527, Length=368, Percent_Identity=35.3260869565217, Blast_Score=235, Evalue=3e-63,
Organism=Escherichia coli, GI1786939, Length=375, Percent_Identity=34.6666666666667, Blast_Score=208, Evalue=6e-55,
Organism=Caenorhabditis elegans, GI17555174, Length=374, Percent_Identity=27.0053475935829, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6319850, Length=366, Percent_Identity=24.3169398907104, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324328, Length=347, Percent_Identity=25.0720461095101, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6325257, Length=364, Percent_Identity=24.7252747252747, Blast_Score=76, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24640126, Length=373, Percent_Identity=28.1501340482574, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24640124, Length=373, Percent_Identity=28.1501340482574, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI21356863, Length=343, Percent_Identity=24.4897959183673, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 41728; Mature: 41596

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVNIYEGALDKGLEGVVACTTKVSFIVGDSLNFRGYTIDDLAANSTFEEVTYLLWNDK
CCCCEEEHHHHHCCCCCEEEHHEEEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHCCCC
LPNAQELAAFSTQLRNEMALSPDFIKVLKAIPTNVHPMGWLRTAVSLMAHWDSDANDNGA
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
EANLRKSVRLTAKMGTLLCAFDAIRKGKEPVAPKADKSMAWNMMYMLGGGAEPKAEHVKV
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHEEECCCCCCHHHHHHH
MDTCLILHADHELNCSAFATRVTASSLSDLHSAIVSAIGALKGPLHGGANEQVILMLQKI
HHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHH
GTMEKAQQFVKDALAAKEKVMGIGHRVYKNGDPRARILRGMSDKLTKDAGIHHMYEMSTL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHCCHHHHHHHHHH
IDDTMFNEKGLMPNVDFYSATVYFSMGIPTDLFTPIFAASRISGWCAHAFEQYANNRIYR
HHHHHHCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEC
PRGKWAGKEGLKWTPANQR
CCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AEVNIYEGALDKGLEGVVACTTKVSFIVGDSLNFRGYTIDDLAANSTFEEVTYLLWNDK
CCCEEEHHHHHCCCCCEEEHHEEEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHCCCC
LPNAQELAAFSTQLRNEMALSPDFIKVLKAIPTNVHPMGWLRTAVSLMAHWDSDANDNGA
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
EANLRKSVRLTAKMGTLLCAFDAIRKGKEPVAPKADKSMAWNMMYMLGGGAEPKAEHVKV
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHEEECCCCCCHHHHHHH
MDTCLILHADHELNCSAFATRVTASSLSDLHSAIVSAIGALKGPLHGGANEQVILMLQKI
HHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHH
GTMEKAQQFVKDALAAKEKVMGIGHRVYKNGDPRARILRGMSDKLTKDAGIHHMYEMSTL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHCCHHHHHHHHHH
IDDTMFNEKGLMPNVDFYSATVYFSMGIPTDLFTPIFAASRISGWCAHAFEQYANNRIYR
HHHHHHCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEC
PRGKWAGKEGLKWTPANQR
CCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8045898; 9387221; 9384377 [H]