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The map label for this gene is dus1 [H]

Identifier: 42521773

GI number: 42521773

Start: 120739

End: 121752

Strand: Direct

Name: dus1 [H]

Synonym: Bd0136

Alternate gene names: 42521773

Gene position: 120739-121752 (Clockwise)

Preceding gene: 42521772

Following gene: 42521774

Centisome position: 3.19

GC content: 50.99

Gene sequence:

>1014_bases
ATGAATCCAGTTGAAGCCTTAAAGACAAATCCCTTTGTCCTTGCCCCTATGGCCGGGATCACGGATCACGCTTTTCGCAC
CTTTATCAAAAAACTAGATGCCAGTGTTGTCGTCACCGAACTTGTCAGTGCCAGCGGTATTGAATACAAGTCCGATCGCA
CTTTGAAGCTGATGAGCTTTGATGAATCTCAGCGCCCCATCGGCATCCAGCTTTTTGGTGAAGAGCCCGAGATCGTGGCG
CGTGCCGCTCAGGTGGCCGAGGCCGATGGCTGTGACTTTGTGGATCTGAACTTTGGTTGTCCTGTTCCGAAAGTCGTGAA
GAAGGGGGCGGGTTCCGCCATTCTGAAAGACCCGGCGGCCGTGCAAAAGATGGTTTCCACCGTCAAAGCGGCAATTAAAA
TTCCTCTGACAATCAAGATCCGCACGGGCTGGGATGCGAATTCCCGCAACGCCACTGAGATTTGTAATATTGCCTACAAC
GAAGGGGTTTCCTGGGTTGCGATCCATGGTCGTACCCGCGCTCAGTCCTATACCGGACTTGCGGACTGGGACTTCATCGC
AGATGTAAAAGCCAATACCAAGATTCCCATTCTTGGAAACGGAGACATCCTCACTCCTCGTCAGGCCAACTTAAGACTGC
AGCAGTCCGGTTGTGACGGGGTGATGATCGGCCGGGGATGCCTTAAAAACCCGTTTATCTTCATGGACGCCCTGTCCTTG
TGGCGAGGCGAGCCGATTAAGGACGTGAAGAGGGATTATGTGAGTCTGTTCCAGGGTCTGCAAAAAGAGATCGTGGCGCA
TTGTGATGAGCACATCACCGGGATCCAGCTCAGAAAGTTTGCAGCTTGGTTCTCAACAGGGTATTCTGGAGCGGCTCAAT
TCCGTAAGAATCTATTCCAGTCCAAAAGCAATGACGAGATCATGGCCTTAGCGAATGAGTTTTTCGCAAGTATTGGAAAT
GTAGAACAAGAAGATACCAGTCAGGAAGATTTCTTAATGGGCGGTCACGGTTAA

Upstream 100 bases:

>100_bases
GGTCATCCGGCCTGAAAAACCTATAAGCTCTCGATTTCTTTTGTTTATTCGGTGGTTTTCCCTATCCATAGAATGGGGAT
TTTGATATATCGCTTCCCCT

Downstream 100 bases:

>100_bases
CAAACCGTGAACTGCGGGGGCCTCTGATGGCGTTTTGACAAGAGCCTGACGAGCATTTGTTTTGATTTTAGTTTAAGTTT
TAAGTGTTTTGTTTTTAGAG

Product: transcription regulator homolog yacF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MNPVEALKTNPFVLAPMAGITDHAFRTFIKKLDASVVVTELVSASGIEYKSDRTLKLMSFDESQRPIGIQLFGEEPEIVA
RAAQVAEADGCDFVDLNFGCPVPKVVKKGAGSAILKDPAAVQKMVSTVKAAIKIPLTIKIRTGWDANSRNATEICNIAYN
EGVSWVAIHGRTRAQSYTGLADWDFIADVKANTKIPILGNGDILTPRQANLRLQQSGCDGVMIGRGCLKNPFIFMDALSL
WRGEPIKDVKRDYVSLFQGLQKEIVAHCDEHITGIQLRKFAAWFSTGYSGAAQFRKNLFQSKSNDEIMALANEFFASIGN
VEQEDTSQEDFLMGGHG

Sequences:

>Translated_337_residues
MNPVEALKTNPFVLAPMAGITDHAFRTFIKKLDASVVVTELVSASGIEYKSDRTLKLMSFDESQRPIGIQLFGEEPEIVA
RAAQVAEADGCDFVDLNFGCPVPKVVKKGAGSAILKDPAAVQKMVSTVKAAIKIPLTIKIRTGWDANSRNATEICNIAYN
EGVSWVAIHGRTRAQSYTGLADWDFIADVKANTKIPILGNGDILTPRQANLRLQQSGCDGVMIGRGCLKNPFIFMDALSL
WRGEPIKDVKRDYVSLFQGLQKEIVAHCDEHITGIQLRKFAAWFSTGYSGAAQFRKNLFQSKSNDEIMALANEFFASIGN
VEQEDTSQEDFLMGGHG
>Mature_337_residues
MNPVEALKTNPFVLAPMAGITDHAFRTFIKKLDASVVVTELVSASGIEYKSDRTLKLMSFDESQRPIGIQLFGEEPEIVA
RAAQVAEADGCDFVDLNFGCPVPKVVKKGAGSAILKDPAAVQKMVSTVKAAIKIPLTIKIRTGWDANSRNATEICNIAYN
EGVSWVAIHGRTRAQSYTGLADWDFIADVKANTKIPILGNGDILTPRQANLRLQQSGCDGVMIGRGCLKNPFIFMDALSL
WRGEPIKDVKRDYVSLFQGLQKEIVAHCDEHITGIQLRKFAAWFSTGYSGAAQFRKNLFQSKSNDEIMALANEFFASIGN
VEQEDTSQEDFLMGGHG

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=227, Percent_Identity=34.3612334801762, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI239788462, Length=227, Percent_Identity=34.3612334801762, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI31742496, Length=240, Percent_Identity=29.1666666666667, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI40807366, Length=334, Percent_Identity=24.5508982035928, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI8923374, Length=281, Percent_Identity=23.8434163701068, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1789660, Length=313, Percent_Identity=33.8658146964856, Blast_Score=181, Evalue=5e-47,
Organism=Escherichia coli, GI1788462, Length=235, Percent_Identity=32.7659574468085, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI145693211, Length=320, Percent_Identity=27.1875, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17543114, Length=239, Percent_Identity=32.6359832635983, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17507177, Length=230, Percent_Identity=32.1739130434783, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI25144369, Length=223, Percent_Identity=32.7354260089686, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17510279, Length=261, Percent_Identity=27.2030651340996, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=232, Percent_Identity=29.7413793103448, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323433, Length=279, Percent_Identity=26.8817204301075, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=247, Percent_Identity=24.2914979757085, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24585320, Length=230, Percent_Identity=34.3478260869565, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24580595, Length=254, Percent_Identity=31.496062992126, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI19920448, Length=254, Percent_Identity=31.496062992126, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI19921524, Length=243, Percent_Identity=25.1028806584362, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36894; Mature: 36894

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPVEALKTNPFVLAPMAGITDHAFRTFIKKLDASVVVTELVSASGIEYKSDRTLKLMSF
CCCHHHHCCCCEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCEECCCCEEEEEEC
DESQRPIGIQLFGEEPEIVARAAQVAEADGCDFVDLNFGCPVPKVVKKGAGSAILKDPAA
CCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHCCCCCCHHCCHHH
VQKMVSTVKAAIKIPLTIKIRTGWDANSRNATEICNIAYNEGVSWVAIHGRTRAQSYTGL
HHHHHHHHHHHEECCEEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
ADWDFIADVKANTKIPILGNGDILTPRQANLRLQQSGCDGVMIGRGCLKNPFIFMDALSL
CCCCCEEECCCCCEEEEEECCCEECCCCCCEEEECCCCCEEEECCCCCCCCHHHHHHHHH
WRGEPIKDVKRDYVSLFQGLQKEIVAHCDEHITGIQLRKFAAWFSTGYSGAAQFRKNLFQ
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
SKSNDEIMALANEFFASIGNVEQEDTSQEDFLMGGHG
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCC
>Mature Secondary Structure
MNPVEALKTNPFVLAPMAGITDHAFRTFIKKLDASVVVTELVSASGIEYKSDRTLKLMSF
CCCHHHHCCCCEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCEECCCCEEEEEEC
DESQRPIGIQLFGEEPEIVARAAQVAEADGCDFVDLNFGCPVPKVVKKGAGSAILKDPAA
CCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHCCCCCCHHCCHHH
VQKMVSTVKAAIKIPLTIKIRTGWDANSRNATEICNIAYNEGVSWVAIHGRTRAQSYTGL
HHHHHHHHHHHEECCEEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
ADWDFIADVKANTKIPILGNGDILTPRQANLRLQQSGCDGVMIGRGCLKNPFIFMDALSL
CCCCCEEECCCCCEEEEEECCCEECCCCCCEEEECCCCCEEEECCCCCCCCHHHHHHHHH
WRGEPIKDVKRDYVSLFQGLQKEIVAHCDEHITGIQLRKFAAWFSTGYSGAAQFRKNLFQ
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
SKSNDEIMALANEFFASIGNVEQEDTSQEDFLMGGHG
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]