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The map label for this gene is groEL

Identifier: 42521743

GI number: 42521743

Start: 91156

End: 92796

Strand: Direct

Name: groEL

Synonym: Bd0099

Alternate gene names: 42521743

Gene position: 91156-92796 (Clockwise)

Preceding gene: 42521742

Following gene: 42521745

Centisome position: 2.41

GC content: 46.68

Gene sequence:

>1641_bases
ATGAGTAAAGTAATCACATTCTCAGAAGACGCTCGTACACACATCCTTAAAGGTGTGAACACTCTGGCGAACGCAGTAAA
AGTAACTTTGGGCCCTAAAGGTCGCAACGTTGTTATCGACAAATCTTTCGGCTCCCCACTGATCACTAAAGACGGTGTGA
CTGTTGCGAAAGAAATCGAACTTGAAAACAAATTCGAAAACATGGGCGCTCAGATGGTTAAGGAAGTTGCTTCCAAAACC
AACGACGAAGCTGGTGACGGTACAACAACTGCAACAGTTTTGGCGCAAGCTATCTACCGTGAAGGCGCGAAACTTGTTTC
CGCTGGTCACAACCCAATGTCTATCAAACGTGGTATCGACAAAGCTGTTGGCATCATCATTGAAGAATTGAAGTCCATGT
CCAAACCGGTAAAAGGTTCCAACGAAGTGGCTCAAGTTGGCGCTATCTCTGCAAACAATGACAAAGAAATCGGTCAGATG
TTGGCAGACGCTATGGATAAAGTAGGCCGTGAAGGCGTTATCACTATCGAAGAATCCAAAACTGCAAAAACGGAAGTGAC
TGTAGTTGAAGGTATGCAATTCGACCGTGGTTACCTTTCTCCATACTTCGTAACCAACGCAGAAAGAATGGAAGCAGTTC
TTGAGAACGCTTACGTTCTTGTTTACGACAAAAAAATCTCTTCCATGAAAGACATGATCGGTATCCTTGAAGGCGTTGCA
AAACAAGGCCGTCAATTGTTGATCATCGCTGAAGACGTTGAAGGCGAAGCATTGGCAACTTTGGTTGTTAATAAGCTGCG
CGGCACTCTGCACATTGCTGCAGTTAAAGCTCCGGGCTTCGGTGACCGTCGTAAAGCTATGCTTGAAGACATCGCGATCC
TGACTGGCGCGAAAGTGATCTCTGAAGACGTTGGTTTGAAACTTGAGGCGGCGACTGTTGCTGACCTTGGTGTTGCAAAA
CGCATCGTTGTAGACAAAGACAACACAACAATCATCGATGGCGCTGGTAAAAAGAACGACATCAATGGCCGTGTTGGTCA
GATCAAAGCTCAGATCGAAGAAACTTCTTCTGACTATGACAAAGAAAAACTGAAAGAGCGTCTGGCTAAATTGGCTGGCG
GTGTTGCAGTTATCCACGTTGGTGCTCCTTCTGAAGTTGAGATGAAAGAGAAAAAACACCGCGTAGAAGACGCTTTGAAC
GCGACTCGCGCTGCGGTTGAAGAAGGTATCGTTGCTGGTGGTGGTACTGCTTTGCTTCGTGCTTCCACTAAAATCGACAA
ATCCAAATTCTCTGAAGAAGAGCAATTCGGTGCGACGATCATCAAACGTGCTTGCGAAGAGCCAATCCGTCAAATCGCGG
CTAACGCTGGTTTGGATGGCGCGATCGTTTTGGATCGTATCCTTCAGAACAAGTCCACGACTTGGGGCTTCAACGCTTAC
TCTGACGAATACACTGATCTGATCAAAGACGGTGTTATCGATCCAGTTAAAGTTGTTCGTTGCGCTTTGACTAACGCAGC
TTCCGTTTCTTCTTTGATGCTGACTACAGAGACTATGATCGCTGAAGCACCTAAGAAAGAATCTGCAGCTCCTGCAATGC
CTGGCCACGACGGTATGGGCGGCATGGGTGGCATGATGTAG

Upstream 100 bases:

>100_bases
ACCTGATGATGCGCGAAGAAGACATCCTGGGCGTATTTAACTAAATCTAAATATCTACTCCAGCGAAGCTGGAGTGAATA
AGAATTGAACGGAGACAATC

Downstream 100 bases:

>100_bases
TCTTCCGACTACTGACTGTGCTTAACGCAAAAAGCCTGGGTTTATAGCCCAGGCTTTTTTTTTAGAACAGGTACTGAACC
CCGCCAAACAACGTTGTCAT

Product: 60 kDa chaperonin

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MSKVITFSEDARTHILKGVNTLANAVKVTLGPKGRNVVIDKSFGSPLITKDGVTVAKEIELENKFENMGAQMVKEVASKT
NDEAGDGTTTATVLAQAIYREGAKLVSAGHNPMSIKRGIDKAVGIIIEELKSMSKPVKGSNEVAQVGAISANNDKEIGQM
LADAMDKVGREGVITIEESKTAKTEVTVVEGMQFDRGYLSPYFVTNAERMEAVLENAYVLVYDKKISSMKDMIGILEGVA
KQGRQLLIIAEDVEGEALATLVVNKLRGTLHIAAVKAPGFGDRRKAMLEDIAILTGAKVISEDVGLKLEAATVADLGVAK
RIVVDKDNTTIIDGAGKKNDINGRVGQIKAQIEETSSDYDKEKLKERLAKLAGGVAVIHVGAPSEVEMKEKKHRVEDALN
ATRAAVEEGIVAGGGTALLRASTKIDKSKFSEEEQFGATIIKRACEEPIRQIAANAGLDGAIVLDRILQNKSTTWGFNAY
SDEYTDLIKDGVIDPVKVVRCALTNAASVSSLMLTTETMIAEAPKKESAAPAMPGHDGMGGMGGMM

Sequences:

>Translated_546_residues
MSKVITFSEDARTHILKGVNTLANAVKVTLGPKGRNVVIDKSFGSPLITKDGVTVAKEIELENKFENMGAQMVKEVASKT
NDEAGDGTTTATVLAQAIYREGAKLVSAGHNPMSIKRGIDKAVGIIIEELKSMSKPVKGSNEVAQVGAISANNDKEIGQM
LADAMDKVGREGVITIEESKTAKTEVTVVEGMQFDRGYLSPYFVTNAERMEAVLENAYVLVYDKKISSMKDMIGILEGVA
KQGRQLLIIAEDVEGEALATLVVNKLRGTLHIAAVKAPGFGDRRKAMLEDIAILTGAKVISEDVGLKLEAATVADLGVAK
RIVVDKDNTTIIDGAGKKNDINGRVGQIKAQIEETSSDYDKEKLKERLAKLAGGVAVIHVGAPSEVEMKEKKHRVEDALN
ATRAAVEEGIVAGGGTALLRASTKIDKSKFSEEEQFGATIIKRACEEPIRQIAANAGLDGAIVLDRILQNKSTTWGFNAY
SDEYTDLIKDGVIDPVKVVRCALTNAASVSSLMLTTETMIAEAPKKESAAPAMPGHDGMGGMGGMM
>Mature_545_residues
SKVITFSEDARTHILKGVNTLANAVKVTLGPKGRNVVIDKSFGSPLITKDGVTVAKEIELENKFENMGAQMVKEVASKTN
DEAGDGTTTATVLAQAIYREGAKLVSAGHNPMSIKRGIDKAVGIIIEELKSMSKPVKGSNEVAQVGAISANNDKEIGQML
ADAMDKVGREGVITIEESKTAKTEVTVVEGMQFDRGYLSPYFVTNAERMEAVLENAYVLVYDKKISSMKDMIGILEGVAK
QGRQLLIIAEDVEGEALATLVVNKLRGTLHIAAVKAPGFGDRRKAMLEDIAILTGAKVISEDVGLKLEAATVADLGVAKR
IVVDKDNTTIIDGAGKKNDINGRVGQIKAQIEETSSDYDKEKLKERLAKLAGGVAVIHVGAPSEVEMKEKKHRVEDALNA
TRAAVEEGIVAGGGTALLRASTKIDKSKFSEEEQFGATIIKRACEEPIRQIAANAGLDGAIVLDRILQNKSTTWGFNAYS
DEYTDLIKDGVIDPVKVVRCALTNAASVSSLMLTTETMIAEAPKKESAAPAMPGHDGMGGMGGMM

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=528, Percent_Identity=50.9469696969697, Blast_Score=523, Evalue=1e-148,
Organism=Homo sapiens, GI31542947, Length=528, Percent_Identity=50.9469696969697, Blast_Score=523, Evalue=1e-148,
Organism=Homo sapiens, GI38455427, Length=139, Percent_Identity=33.0935251798561, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI5453607, Length=549, Percent_Identity=22.5865209471767, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI24307939, Length=130, Percent_Identity=31.5384615384615, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI1790586, Length=529, Percent_Identity=62.1928166351607, Blast_Score=654, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=529, Percent_Identity=50.0945179584121, Blast_Score=519, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI193210679, Length=212, Percent_Identity=47.6415094339623, Blast_Score=203, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI25144674, Length=194, Percent_Identity=31.4432989690722, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI25147750, Length=130, Percent_Identity=35.3846153846154, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323288, Length=524, Percent_Identity=52.0992366412214, Blast_Score=520, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6322524, Length=134, Percent_Identity=31.3432835820896, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24641193, Length=528, Percent_Identity=53.030303030303, Blast_Score=548, Evalue=1e-156,
Organism=Drosophila melanogaster, GI24641191, Length=528, Percent_Identity=53.030303030303, Blast_Score=548, Evalue=1e-156,
Organism=Drosophila melanogaster, GI45550936, Length=528, Percent_Identity=48.6742424242424, Blast_Score=498, Evalue=1e-141,
Organism=Drosophila melanogaster, GI45550132, Length=528, Percent_Identity=48.6742424242424, Blast_Score=498, Evalue=1e-141,
Organism=Drosophila melanogaster, GI45550935, Length=528, Percent_Identity=48.6742424242424, Blast_Score=498, Evalue=1e-141,
Organism=Drosophila melanogaster, GI17864606, Length=525, Percent_Identity=44.952380952381, Blast_Score=449, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24584129, Length=534, Percent_Identity=37.4531835205993, Blast_Score=329, Evalue=3e-90,
Organism=Drosophila melanogaster, GI19921262, Length=534, Percent_Identity=37.4531835205993, Blast_Score=329, Evalue=3e-90,
Organism=Drosophila melanogaster, GI17647245, Length=134, Percent_Identity=30.5970149253731, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24645179, Length=518, Percent_Identity=21.2355212355212, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24652903, Length=124, Percent_Identity=30.6451612903226, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24583944, Length=129, Percent_Identity=29.4573643410853, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH60_BDEBA (Q6MRI1)

Other databases:

- EMBL:   BX842646
- RefSeq:   NP_967123.1
- ProteinModelPortal:   Q6MRI1
- SMR:   Q6MRI1
- GeneID:   2733063
- GenomeReviews:   BX842601_GR
- KEGG:   bba:Bd0099
- NMPDR:   fig|264462.1.peg.93
- HOGENOM:   HBG625289
- OMA:   NSDTSIG
- PhylomeDB:   Q6MRI1
- BioCyc:   BBAC264462:BD0099-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 58129; Mature: 57997

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVITFSEDARTHILKGVNTLANAVKVTLGPKGRNVVIDKSFGSPLITKDGVTVAKEIE
CCCEEECCCHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCEECCCCCCHHHHH
LENKFENMGAQMVKEVASKTNDEAGDGTTTATVLAQAIYREGAKLVSAGHNPMSIKRGID
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHH
KAVGIIIEELKSMSKPVKGSNEVAQVGAISANNDKEIGQMLADAMDKVGREGVITIEESK
HHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCC
TAKTEVTVVEGMQFDRGYLSPYFVTNAERMEAVLENAYVLVYDKKISSMKDMIGILEGVA
CCCEEEEEECCCCCCCCCCCCEEECCHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHH
KQGRQLLIIAEDVEGEALATLVVNKLRGTLHIAAVKAPGFGDRRKAMLEDIAILTGAKVI
HCCCEEEEEEECCCCHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
SEDVGLKLEAATVADLGVAKRIVVDKDNTTIIDGAGKKNDINGRVGQIKAQIEETSSDYD
HHCCCCEEEHHHHHHHHHHEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCC
KEKLKERLAKLAGGVAVIHVGAPSEVEMKEKKHRVEDALNATRAAVEEGIVAGGGTALLR
HHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
ASTKIDKSKFSEEEQFGATIIKRACEEPIRQIAANAGLDGAIVLDRILQNKSTTWGFNAY
EHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCC
SDEYTDLIKDGVIDPVKVVRCALTNAASVSSLMLTTETMIAEAPKKESAAPAMPGHDGMG
CHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
GMGGMM
CCCCCC
>Mature Secondary Structure 
SKVITFSEDARTHILKGVNTLANAVKVTLGPKGRNVVIDKSFGSPLITKDGVTVAKEIE
CCEEECCCHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCEECCCCCCHHHHH
LENKFENMGAQMVKEVASKTNDEAGDGTTTATVLAQAIYREGAKLVSAGHNPMSIKRGID
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHH
KAVGIIIEELKSMSKPVKGSNEVAQVGAISANNDKEIGQMLADAMDKVGREGVITIEESK
HHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCC
TAKTEVTVVEGMQFDRGYLSPYFVTNAERMEAVLENAYVLVYDKKISSMKDMIGILEGVA
CCCEEEEEECCCCCCCCCCCCEEECCHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHH
KQGRQLLIIAEDVEGEALATLVVNKLRGTLHIAAVKAPGFGDRRKAMLEDIAILTGAKVI
HCCCEEEEEEECCCCHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
SEDVGLKLEAATVADLGVAKRIVVDKDNTTIIDGAGKKNDINGRVGQIKAQIEETSSDYD
HHCCCCEEEHHHHHHHHHHEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCC
KEKLKERLAKLAGGVAVIHVGAPSEVEMKEKKHRVEDALNATRAAVEEGIVAGGGTALLR
HHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
ASTKIDKSKFSEEEQFGATIIKRACEEPIRQIAANAGLDGAIVLDRILQNKSTTWGFNAY
EHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCC
SDEYTDLIKDGVIDPVKVVRCALTNAASVSSLMLTTETMIAEAPKKESAAPAMPGHDGMG
CHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
GMGGMM
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA