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The map label for this gene is gatA

Identifier: 42521706

GI number: 42521706

Start: 52978

End: 54450

Strand: Direct

Name: gatA

Synonym: Bd0059

Alternate gene names: 42521706

Gene position: 52978-54450 (Clockwise)

Preceding gene: 42521705

Following gene: 42521707

Centisome position: 1.4

GC content: 53.02

Gene sequence:

>1473_bases
GTGGATCTAACATTTGCCTCGCTTTCTGAAATTTCTGAAGCCGTCAATAACCGCAGTATCAGCGCCAAAGAAGTCACTTT
GCATTTCCTAAAGCGCATTGAAAATTTGAATCCGAAGCTGAATGCCTTTACGTCACTAAATCCCCAGGCTGTTCAGGAGG
CTGAGGCTGTAGACGCTCGAATCGCCAACGGCGAAGACGTGGGCCTGCTGGCGGGTGTGCCTTTTGGTATCAAAGAGATG
TTTTGTACCAAGGGTCTGACAACCACCGCCGGATCCAAGATTCTTGAAAACTTTGTTCCGCCTTATGATGCCACAGCGGT
GGCCCGTCTTAAAAAATCCGGCATCGTGGTGATGGGTAAGCTGAATCAGGATGAATTTGCCATGGGCTCTTCCAATGAAA
CGTCCTTTCACGGTGTGGTGAAAAACCCCTGGGACCTGGAACGTGTGCCCGGGGGATCTTCCGGTGGCTCGGCGGCAGCG
CAAGCGTCGCGCCTGGTGGCAGGCACGCTGGGTACCGACACCGGCGGCTCGATTCGCCAACCCGCAAGCTTCTGCGGTAT
CGTGGGCGTGAAGCCCACATACGGCCGTGTGAGCCGCTATGGCATTGTCGCTTATGCTTCGTCCTTGGATCAGGCCGGCC
CGATGGTGAGTTCTGTGAGGGATGCGGCATTGACCCTGGAAGTGATTTCAGGTTTTGACCCGCAGGACTCCACCACAACT
CAGAAACAAGTCCCCGCCTGGAGTCAGAATCTGAAAGCCGACGTCAAAGGCATGAAGATCGGTCTGATGAAGGAGTACAT
GACTGGAGCTTTGGATCCGGACGTGCAAAAGACTGTGGAAAACTCGGTGGATACCTTAAAACAACTCGGAGCTGAGATTG
TTGAAGTCTCTGTTCCCATGACCGCGTTTGCAGTTCCTGTGTATTACCTGGTGGCCGCCAGCGAAGCGTCGTCGAATCTT
TCCCGCTATGACGGTGTGAAATACGGCTATCGTGCCGAATTTAAAAATCTTTCGGCGGTGGACCTGGAAGAGTTCTACAG
TCAGACGCGGGGTCAGGCGTTCGGTGCCGAAGTCAAACGTCGTATCATGCTGGGCACGTACTGCCTTTCCAGCGGTTACT
ATGATGCCTTTTACAACAAGGCCGGTCAGGTGCGCCGCCTGATCATGGAACAGTATCTGGAAGCCTTCAAAAAATGTGAT
GTGATTTTAAGTCCGGTCACCACCGCACCCGCCTTCAAGATTGGTGAACGGGTTTCTGATCCGTTGGCGATGTATTTGAA
TGACATCTTTACCACCTCGACGAATCTTGCCGGACTTCCGGGAATGAGTGTCCCGTTTGGTCAGTCGCAAAGTGGTCTTC
CGATCGGGATTCAGCTGACCGCCGGTCACTTTGAAGAACAAAAGATGCTGAACGTGGCCTTTGCGCTGGAAGGTGCCTCT
TTGGTGAAAGGAAAACATCCCCATGTCATCTAG

Upstream 100 bases:

>100_bases
TGTTGTGAAGCAGGATTTCACCGCCGAAGAAATGACCGGAAATGCACCGGACCGTGCGGGCAATCTTTTTAAAGTCCCAC
CGGTCGTTTAGGAGGTTGTT

Downstream 100 bases:

>100_bases
AGGATATGAAGCCGTCATCGGTATTGAGATCCACGTGCAGCTGAGCACAAAGACCAAAATCTTCAGCTCCGAATCCACGG
CCTTTGAAGCGGGTGACAAT

Product: glutamyl-tRNA(Gln) amidotransferase, A subunit

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MDLTFASLSEISEAVNNRSISAKEVTLHFLKRIENLNPKLNAFTSLNPQAVQEAEAVDARIANGEDVGLLAGVPFGIKEM
FCTKGLTTTAGSKILENFVPPYDATAVARLKKSGIVVMGKLNQDEFAMGSSNETSFHGVVKNPWDLERVPGGSSGGSAAA
QASRLVAGTLGTDTGGSIRQPASFCGIVGVKPTYGRVSRYGIVAYASSLDQAGPMVSSVRDAALTLEVISGFDPQDSTTT
QKQVPAWSQNLKADVKGMKIGLMKEYMTGALDPDVQKTVENSVDTLKQLGAEIVEVSVPMTAFAVPVYYLVAASEASSNL
SRYDGVKYGYRAEFKNLSAVDLEEFYSQTRGQAFGAEVKRRIMLGTYCLSSGYYDAFYNKAGQVRRLIMEQYLEAFKKCD
VILSPVTTAPAFKIGERVSDPLAMYLNDIFTTSTNLAGLPGMSVPFGQSQSGLPIGIQLTAGHFEEQKMLNVAFALEGAS
LVKGKHPHVI

Sequences:

>Translated_490_residues
MDLTFASLSEISEAVNNRSISAKEVTLHFLKRIENLNPKLNAFTSLNPQAVQEAEAVDARIANGEDVGLLAGVPFGIKEM
FCTKGLTTTAGSKILENFVPPYDATAVARLKKSGIVVMGKLNQDEFAMGSSNETSFHGVVKNPWDLERVPGGSSGGSAAA
QASRLVAGTLGTDTGGSIRQPASFCGIVGVKPTYGRVSRYGIVAYASSLDQAGPMVSSVRDAALTLEVISGFDPQDSTTT
QKQVPAWSQNLKADVKGMKIGLMKEYMTGALDPDVQKTVENSVDTLKQLGAEIVEVSVPMTAFAVPVYYLVAASEASSNL
SRYDGVKYGYRAEFKNLSAVDLEEFYSQTRGQAFGAEVKRRIMLGTYCLSSGYYDAFYNKAGQVRRLIMEQYLEAFKKCD
VILSPVTTAPAFKIGERVSDPLAMYLNDIFTTSTNLAGLPGMSVPFGQSQSGLPIGIQLTAGHFEEQKMLNVAFALEGAS
LVKGKHPHVI
>Mature_490_residues
MDLTFASLSEISEAVNNRSISAKEVTLHFLKRIENLNPKLNAFTSLNPQAVQEAEAVDARIANGEDVGLLAGVPFGIKEM
FCTKGLTTTAGSKILENFVPPYDATAVARLKKSGIVVMGKLNQDEFAMGSSNETSFHGVVKNPWDLERVPGGSSGGSAAA
QASRLVAGTLGTDTGGSIRQPASFCGIVGVKPTYGRVSRYGIVAYASSLDQAGPMVSSVRDAALTLEVISGFDPQDSTTT
QKQVPAWSQNLKADVKGMKIGLMKEYMTGALDPDVQKTVENSVDTLKQLGAEIVEVSVPMTAFAVPVYYLVAASEASSNL
SRYDGVKYGYRAEFKNLSAVDLEEFYSQTRGQAFGAEVKRRIMLGTYCLSSGYYDAFYNKAGQVRRLIMEQYLEAFKKCD
VILSPVTTAPAFKIGERVSDPLAMYLNDIFTTSTNLAGLPGMSVPFGQSQSGLPIGIQLTAGHFEEQKMLNVAFALEGAS
LVKGKHPHVI

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=497, Percent_Identity=36.0160965794769, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI195972892, Length=498, Percent_Identity=26.3052208835341, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI166795287, Length=192, Percent_Identity=34.375, Blast_Score=77, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=415, Percent_Identity=37.3493975903614, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17537465, Length=498, Percent_Identity=23.2931726907631, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17556264, Length=297, Percent_Identity=26.9360269360269, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71990152, Length=311, Percent_Identity=30.2250803858521, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17556278, Length=298, Percent_Identity=28.8590604026846, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17556276, Length=298, Percent_Identity=28.8590604026846, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=310, Percent_Identity=24.5161290322581, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323950, Length=464, Percent_Identity=33.6206896551724, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6319685, Length=474, Percent_Identity=27.0042194092827, Blast_Score=104, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648113, Length=493, Percent_Identity=38.3367139959432, Blast_Score=312, Evalue=4e-85,
Organism=Drosophila melanogaster, GI24652985, Length=508, Percent_Identity=27.3622047244095, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI19922090, Length=508, Percent_Identity=27.3622047244095, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24652981, Length=508, Percent_Identity=27.3622047244095, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24652983, Length=508, Percent_Identity=27.3622047244095, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI161078093, Length=499, Percent_Identity=27.4549098196393, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24648435, Length=502, Percent_Identity=26.2948207171315, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24648437, Length=502, Percent_Identity=26.2948207171315, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI45550774, Length=502, Percent_Identity=26.2948207171315, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24644968, Length=241, Percent_Identity=31.9502074688797, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI21356731, Length=231, Percent_Identity=32.9004329004329, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648441, Length=439, Percent_Identity=26.879271070615, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24648439, Length=439, Percent_Identity=26.879271070615, Blast_Score=92, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_BDEBA (Q6MRL7)

Other databases:

- EMBL:   BX842646
- RefSeq:   NP_967086.1
- ProteinModelPortal:   Q6MRL7
- SMR:   Q6MRL7
- GeneID:   2733048
- GenomeReviews:   BX842601_GR
- KEGG:   bba:Bd0059
- NMPDR:   fig|264462.1.peg.56
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- PhylomeDB:   Q6MRL7
- BioCyc:   BBAC264462:BD0059-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52535; Mature: 52535

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 78-78 ACT_SITE 153-153 ACT_SITE 177-177

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLTFASLSEISEAVNNRSISAKEVTLHFLKRIENLNPKLNAFTSLNPQAVQEAEAVDAR
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHC
IANGEDVGLLAGVPFGIKEMFCTKGLTTTAGSKILENFVPPYDATAVARLKKSGIVVMGK
CCCCCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEEE
LNQDEFAMGSSNETSFHGVVKNPWDLERVPGGSSGGSAAAQASRLVAGTLGTDTGGSIRQ
CCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCCCCCCC
PASFCGIVGVKPTYGRVSRYGIVAYASSLDQAGPMVSSVRDAALTLEVISGFDPQDSTTT
CHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCH
QKQVPAWSQNLKADVKGMKIGLMKEYMTGALDPDVQKTVENSVDTLKQLGAEIVEVSVPM
HHHCCHHHHCCHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCH
TAFAVPVYYLVAASEASSNLSRYDGVKYGYRAEFKNLSAVDLEEFYSQTRGQAFGAEVKR
HHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHCCCCCCCCHHHHHHHHCCCHHHHHHHH
RIMLGTYCLSSGYYDAFYNKAGQVRRLIMEQYLEAFKKCDVILSPVTTAPAFKIGERVSD
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHCC
PLAMYLNDIFTTSTNLAGLPGMSVPFGQSQSGLPIGIQLTAGHFEEQKMLNVAFALEGAS
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHEECCHH
LVKGKHPHVI
HHCCCCCCCC
>Mature Secondary Structure
MDLTFASLSEISEAVNNRSISAKEVTLHFLKRIENLNPKLNAFTSLNPQAVQEAEAVDAR
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHC
IANGEDVGLLAGVPFGIKEMFCTKGLTTTAGSKILENFVPPYDATAVARLKKSGIVVMGK
CCCCCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEEE
LNQDEFAMGSSNETSFHGVVKNPWDLERVPGGSSGGSAAAQASRLVAGTLGTDTGGSIRQ
CCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCCCCCCC
PASFCGIVGVKPTYGRVSRYGIVAYASSLDQAGPMVSSVRDAALTLEVISGFDPQDSTTT
CHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCH
QKQVPAWSQNLKADVKGMKIGLMKEYMTGALDPDVQKTVENSVDTLKQLGAEIVEVSVPM
HHHCCHHHHCCHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCH
TAFAVPVYYLVAASEASSNLSRYDGVKYGYRAEFKNLSAVDLEEFYSQTRGQAFGAEVKR
HHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHCCCCCCCCHHHHHHHHCCCHHHHHHHH
RIMLGTYCLSSGYYDAFYNKAGQVRRLIMEQYLEAFKKCDVILSPVTTAPAFKIGERVSD
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHCC
PLAMYLNDIFTTSTNLAGLPGMSVPFGQSQSGLPIGIQLTAGHFEEQKMLNVAFALEGAS
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHEECCHH
LVKGKHPHVI
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA