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The map label for this gene is pvuIIM [H]

Identifier: 38637986

GI number: 38637986

Start: 339567

End: 340808

Strand: Direct

Name: pvuIIM [H]

Synonym: PHG325

Alternate gene names: 38637986

Gene position: 339567-340808 (Clockwise)

Preceding gene: 38637985

Following gene: 38637987

Centisome position: 75.1

GC content: 64.01

Gene sequence:

>1242_bases
ATGCGCCAACTAGACCTGTTCGCACACGTCGCCGCGGCGTTTGCAGACGCGTCCGAAGATGGGCTCTCCACCGGCGCTCT
CTACGATATTGTGGCGCGTTCGGCGGGCATCGATCACAGCGCGGCCACGGCGCGCGTTCCGATCGGGACTGCAAAGGCAC
TGCATAGCCCATTCAAGCGTGCGGTTCGCTGGCACCAGCAGACGCTCAAGACGCTCGGTGTTGTCGAGCGTGTCGCCGAT
CGCACGGGATTCTGGCGCCTCACGCAGCCGGTCACCAAGGACCTCGACCGTGCAGCTGACGGCGTCAAGCTGGTCGCGTT
CAGCACGATGCTCGGCATGGCCATCTGGGCTCGAAATGAGGATGTGTTTCGAGGATTGGCGGAACCCATCGCCTTGTGCG
TCACGTCGCCGCCATACCCACTGCGGCAAGCCAGGGCATATGGCAATCCTACGGATAACCAGTATGTCGACTTCATCTGC
GGGGCGCTCGAGCCGATCGTTGCCAATCTCGTGACCGGCGGCAGCATCGTCCTGAACATTTCCAACGACATCTTCCAGCA
CCGCAGTCCCGCCCGATCCCTCTATATGGAGCGGCTTGTGCTGGCCCTTCATGACCGACTCGGCTTGGCGCTGATGGACC
GCATTCCATGGGTGAACTACAGCAAGCCGCCGGGCCCGACTCGATGGGCATGCGTGGACCGTGTCCACCTCGCAACGGCA
TACGAGCCATTACTATGGCTGACGAACGATCCAGGCCGTGTCCGTGCCGACAACCGCCGCGTGCTGCAGGCCCATACCGA
ACGGCATCGGCGGCTGCTCGAAGCCGGTGGCGAACAGCGGTCAGCCAGTTACGGTGATGGTGCCTATCGGATCCGACCCC
AGTCCTTTGGCAATCCGACATCCGGCCGGCTTTCCCGCAATGTCGTCGAACGCGGCCACCGCTGCGCCGACACCCTGGCC
TACCGCAGGGCGGCCGCGACGCTTGGATTGCCCGCGCAAGGCGCCATGCAGCCTACCGCCATCCCGGACTTCTTTACAAG
ATTCCTGACCGAGCCTGGCGACCTGGTCGTCGATCCGTTCGGCGGCACGATCCGTACGGGCCTCGCCGCCGAGCGATTGG
GCAGGCGCTGGATCGTCACGGAATGGATCCTCCAGTATGCTCGCGGCGCCGCCGAGCTGTTTCGTCAGTCCGACGGCTTC
CTGATGCACCCCGCCCTCCAATGGGCTTCCCAAGGTCGATAG

Upstream 100 bases:

>100_bases
CAGGCCGGCAACTCGACGGGATGCTGCATGACGCTTTCCCTGTGACCTCGTTGGACGTGGCGGAGGCCTGCCACCGCGCC
CTGGGTCGTGGAGCAGTCTG

Downstream 100 bases:

>100_bases
CAATGCAGCACCACGACAACGAAGACAGAGAATTTCCCGAGCCCGAGACCGTCCTTGCCATTCGGGGGGCAATCGCCACC
GGCCGGATGGGCGGCCCGAT

Product: putative DNA methyltransferase

Products: NA

Alternate protein names: M.PvuII; N-4 cytosine-specific methyltransferase PvuII [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MRQLDLFAHVAAAFADASEDGLSTGALYDIVARSAGIDHSAATARVPIGTAKALHSPFKRAVRWHQQTLKTLGVVERVAD
RTGFWRLTQPVTKDLDRAADGVKLVAFSTMLGMAIWARNEDVFRGLAEPIALCVTSPPYPLRQARAYGNPTDNQYVDFIC
GALEPIVANLVTGGSIVLNISNDIFQHRSPARSLYMERLVLALHDRLGLALMDRIPWVNYSKPPGPTRWACVDRVHLATA
YEPLLWLTNDPGRVRADNRRVLQAHTERHRRLLEAGGEQRSASYGDGAYRIRPQSFGNPTSGRLSRNVVERGHRCADTLA
YRRAAATLGLPAQGAMQPTAIPDFFTRFLTEPGDLVVDPFGGTIRTGLAAERLGRRWIVTEWILQYARGAAELFRQSDGF
LMHPALQWASQGR

Sequences:

>Translated_413_residues
MRQLDLFAHVAAAFADASEDGLSTGALYDIVARSAGIDHSAATARVPIGTAKALHSPFKRAVRWHQQTLKTLGVVERVAD
RTGFWRLTQPVTKDLDRAADGVKLVAFSTMLGMAIWARNEDVFRGLAEPIALCVTSPPYPLRQARAYGNPTDNQYVDFIC
GALEPIVANLVTGGSIVLNISNDIFQHRSPARSLYMERLVLALHDRLGLALMDRIPWVNYSKPPGPTRWACVDRVHLATA
YEPLLWLTNDPGRVRADNRRVLQAHTERHRRLLEAGGEQRSASYGDGAYRIRPQSFGNPTSGRLSRNVVERGHRCADTLA
YRRAAATLGLPAQGAMQPTAIPDFFTRFLTEPGDLVVDPFGGTIRTGLAAERLGRRWIVTEWILQYARGAAELFRQSDGF
LMHPALQWASQGR
>Mature_413_residues
MRQLDLFAHVAAAFADASEDGLSTGALYDIVARSAGIDHSAATARVPIGTAKALHSPFKRAVRWHQQTLKTLGVVERVAD
RTGFWRLTQPVTKDLDRAADGVKLVAFSTMLGMAIWARNEDVFRGLAEPIALCVTSPPYPLRQARAYGNPTDNQYVDFIC
GALEPIVANLVTGGSIVLNISNDIFQHRSPARSLYMERLVLALHDRLGLALMDRIPWVNYSKPPGPTRWACVDRVHLATA
YEPLLWLTNDPGRVRADNRRVLQAHTERHRRLLEAGGEQRSASYGDGAYRIRPQSFGNPTSGRLSRNVVERGHRCADTLA
YRRAAATLGLPAQGAMQPTAIPDFFTRFLTEPGDLVVDPFGGTIRTGLAAERLGRRWIVTEWILQYARGAAELFRQSDGF
LMHPALQWASQGR

Specific function: This methylase recognizes the double-stranded sequence CAGCTG, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the PvuII endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 45698; Mature: 45698

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQLDLFAHVAAAFADASEDGLSTGALYDIVARSAGIDHSAATARVPIGTAKALHSPFKR
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHH
AVRWHQQTLKTLGVVERVADRTGFWRLTQPVTKDLDRAADGVKLVAFSTMLGMAIWARNE
HHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCH
DVFRGLAEPIALCVTSPPYPLRQARAYGNPTDNQYVDFICGALEPIVANLVTGGSIVLNI
HHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEE
SNDIFQHRSPARSLYMERLVLALHDRLGLALMDRIPWVNYSKPPGPTRWACVDRVHLATA
CHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
YEPLLWLTNDPGRVRADNRRVLQAHTERHRRLLEAGGEQRSASYGDGAYRIRPQSFGNPT
HCCEEEEECCCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCC
SGRLSRNVVERGHRCADTLAYRRAAATLGLPAQGAMQPTAIPDFFTRFLTEPGDLVVDPF
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEECC
GGTIRTGLAAERLGRRWIVTEWILQYARGAAELFRQSDGFLMHPALQWASQGR
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHCCCC
>Mature Secondary Structure
MRQLDLFAHVAAAFADASEDGLSTGALYDIVARSAGIDHSAATARVPIGTAKALHSPFKR
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHH
AVRWHQQTLKTLGVVERVADRTGFWRLTQPVTKDLDRAADGVKLVAFSTMLGMAIWARNE
HHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCH
DVFRGLAEPIALCVTSPPYPLRQARAYGNPTDNQYVDFICGALEPIVANLVTGGSIVLNI
HHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEE
SNDIFQHRSPARSLYMERLVLALHDRLGLALMDRIPWVNYSKPPGPTRWACVDRVHLATA
CHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
YEPLLWLTNDPGRVRADNRRVLQAHTERHRRLLEAGGEQRSASYGDGAYRIRPQSFGNPT
HCCEEEEECCCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCC
SGRLSRNVVERGHRCADTLAYRRAAATLGLPAQGAMQPTAIPDFFTRFLTEPGDLVVDPF
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEECC
GGTIRTGLAAERLGRRWIVTEWILQYARGAAELFRQSDGFLMHPALQWASQGR
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2662138; 7607491; 9207015 [H]