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The map label for this gene is hypF2

Identifier: 38637761

GI number: 38637761

Start: 86804

End: 89227

Strand: Direct

Name: hypF2

Synonym: PHG096

Alternate gene names: 38637761

Gene position: 86804-89227 (Clockwise)

Preceding gene: 38637760

Following gene: 38637762

Centisome position: 19.2

GC content: 71.99

Gene sequence:

>2424_bases
ATGCTGATGCCGCGCCGTCCTCGCAATCCGCGCACCGTCCGCATCCGCATCCGCGTGCGCGGCGTCGTGCAGGGCGTGGG
CTTCCGGCCCTTCGTCTATCGCCTGGCGCGGGAGCTGGGCCTCGCCGGCTGGGTGCGCAACGACGGCGCCGGCGTGGACA
TCGAGGCCCAAGGGAGCGCCGCGGCCCTCGTCGAGCTGCGCGAGCGCCTGCGCCGCGACGCGCCGCCCCTGGCGCGGGTG
GATGAGATCGGTGAGGAACGCTGTGCGGCACAGGTTGACGCCGACGGCTTTGCCATCCTCGAGAGCAGCCGTTCCGATGC
CGCCGTGCACACCGCCATCGGCCACGACACGGCCGTATGCCCCGACTGCCTGGCGGAGCTGTTCGACCCCGCGAACCGGC
GCTACCGCTACGCCTTCATCAACTGCACCCAGTGCGGCCCGCGCTACACCCTGACGTGGGCGCTGCCCTACGACCGCGCC
ACCACCAGCATGGCGCCGTTCCCCCAGTGCCGGCCCTGTCTCGACGAGTATAACGCACCCGAGCATCGCCGCTTCCACGC
CGAGCCCAACGCCTGTCCGGACTGTGGCCCCAGCCTGGCGCTGCTCAATGCCCAGGGCATGCCGGTCGAGGACGTTGATC
CGATCGCCGAGACGGTGGCGCGCCTGCAGCGGGGCGAGATCGTCGCCATCAAGGGCCTTGGGGGCTTCCATCTCGCCTGT
GACGCGCACAATGCCGACGCGGTGGCGCGGCTGCGCAGCCGCAAGCAGCGCGAGGAGAAGCCCTTCGCCGTCATGGTCGC
GAACCTGGCGACGGCGGCGCAGTGGGGCGACATCGGCAGCGGTGAAGCGGCCCTGCTCACGGCGTCGGAGCGGCCCATCG
TTCTGCTGCGCAAGCGCAGCGGCGTCGATGGGCGGTTCGCGGGCGTGGCGCCGGGACTGGTCTGGCTGGGCGTCATGCTG
CCGTACACGCCGCTCCAGTATCTGCTGTTCCACGAGGCCGCTGGCCGCCCCGAGGGCCTCGGCTGGCTCGCCCAGCCGCA
GTCGCTGGTGCTGGTGATGACCAGCGCCAACCCCGGCGGCGAGCCCTTGGTCACCGGCAACGACGAGGCGGCGCAGCGGC
TCACTGGCATCGCCGACGCTTTCCTGCTCCACGATCGCGAGATCCTGGTGCGTTGTGACGATTCGGTGGTGAGGGGCGAC
GGCGAACCAGCGCCTCACGTCCAGTTCATTCGCCGCGCGCGGGGTTACACGCCGCGGGCGATCAAGCTGGCGCGCAGCGG
CCCCTCGGTGCTGGCGCTGGGCGGCTCCTTCAAGAACACGGTGTGCCTGACGCGCGGCGACGAGGCCTTCGTCTCTCAGC
ATGTGGGCGATCTCGGCAACGCTGCCACCTGCGAGGCCCTGATCGAGGCGGTGGCCCATCTGCAGCGGGTGCTGGAGATC
CGGCCGCAGCTCGTCGCCCACGATCTGCATCCGGACTTCTTCAGCACACGCCACGCAGCGGAGCTGGCGGCGCAATGGGG
GGTGCCTGCCGTCGCCGTGCAGCACCATCATGCCCATATTGCGGCGGTGCTGGCCGAGCACGGCTCGGACGAACCCGCCA
TCGGCCTGGCGCTGGACGGCGTCGGCCTGGGCGACGACGGGCAGGCGTGGGGTGGCGAACTGCTGCTGGTGGACGGCGGC
GCCTGTAAGCGCCTCGGTCACTTGCGCGAGCTGCCGCTACCTGGCGGCGATCGCGCCGCGCGGGAGCCCTGGCGCATGGC
CGCTGCGGCGCTGCATGCGATGGGCCGCGGCGAGGAGATCGAGGGGCGCTTCCCGCGGCAGCCGGGGGCGCCGATGGTGA
ACCGCATGCTGGCCCAGCGGCTTAACGCGCCCCTCTCCTCCAGCATGGGCCGCTGGTTCGACGCCGCCGCCGGCCTGCTC
GGCACACGGGAAACCATGGCCTACGAGGGGCAGGCGGCGATGCTGCTGGAAGGGTTGGCCGAGAGCTGGGGCGAGCAACC
GTCGCCGGGCCGCCCCAAGACGGTTGCTCACTCCCTCGGGGGGGTGCCGCGCAGCGGCGGGGGTACATACAAGGCGCTGG
CGCTGCCCGATGCCTGGCGCATCGATGCGGGCAACACCCTGGATCTGCTGCCGCTGCTCGAGGCGCTGTCCGCCGAAACG
AATGCCGCGCGCGGCGCGGCACAGTTCCACGCCACCCTGGTGGCGGCGCTGGAAGCCTGGACCGTCGCGACCGTCCAGGT
CACCGGCGTGCGCACGGTCGTCTTCGGCGGCGGCTGCTTCCTCAACCACATCCTCGCCCGCAACCTGTGCCGGCGCCTGG
CGGCGCGGGGACTGACCGTGCTGACAGCGCGCCAGCTGCCGCCCAATGACGGAGGCATTGCCCTCGGTCAGGTCTGGGTG
GCGCTGCAGCGGGCGCCCAACTGA

Upstream 100 bases:

>100_bases
TGGCCGGCCAGGCCGCCAGGCGCAGCCAGAGCATGGAGCTACTGCGCAGCCGCATCGCCGGGCTGGAAGCGCAACTGGCG
GCGCTCAAGGTGTAGCGGCC

Downstream 100 bases:

>100_bases
CGAGAGGGTAGAACATGTGCCTGGCCATACCTGCACGCATCGCGAAAAAATTTGACAACGACATGGCCCTCATCGACCTG
GGCGGCGTGGGGAGTGGCCA

Product: HypF2

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF2; [NiFe]-hydrogenase maturation factor hypF2; Hydrogenase maturation protein hypF2

Number of amino acids: Translated: 807; Mature: 807

Protein sequence:

>807_residues
MLMPRRPRNPRTVRIRIRVRGVVQGVGFRPFVYRLARELGLAGWVRNDGAGVDIEAQGSAAALVELRERLRRDAPPLARV
DEIGEERCAAQVDADGFAILESSRSDAAVHTAIGHDTAVCPDCLAELFDPANRRYRYAFINCTQCGPRYTLTWALPYDRA
TTSMAPFPQCRPCLDEYNAPEHRRFHAEPNACPDCGPSLALLNAQGMPVEDVDPIAETVARLQRGEIVAIKGLGGFHLAC
DAHNADAVARLRSRKQREEKPFAVMVANLATAAQWGDIGSGEAALLTASERPIVLLRKRSGVDGRFAGVAPGLVWLGVML
PYTPLQYLLFHEAAGRPEGLGWLAQPQSLVLVMTSANPGGEPLVTGNDEAAQRLTGIADAFLLHDREILVRCDDSVVRGD
GEPAPHVQFIRRARGYTPRAIKLARSGPSVLALGGSFKNTVCLTRGDEAFVSQHVGDLGNAATCEALIEAVAHLQRVLEI
RPQLVAHDLHPDFFSTRHAAELAAQWGVPAVAVQHHHAHIAAVLAEHGSDEPAIGLALDGVGLGDDGQAWGGELLLVDGG
ACKRLGHLRELPLPGGDRAAREPWRMAAAALHAMGRGEEIEGRFPRQPGAPMVNRMLAQRLNAPLSSSMGRWFDAAAGLL
GTRETMAYEGQAAMLLEGLAESWGEQPSPGRPKTVAHSLGGVPRSGGGTYKALALPDAWRIDAGNTLDLLPLLEALSAET
NAARGAAQFHATLVAALEAWTVATVQVTGVRTVVFGGGCFLNHILARNLCRRLAARGLTVLTARQLPPNDGGIALGQVWV
ALQRAPN

Sequences:

>Translated_807_residues
MLMPRRPRNPRTVRIRIRVRGVVQGVGFRPFVYRLARELGLAGWVRNDGAGVDIEAQGSAAALVELRERLRRDAPPLARV
DEIGEERCAAQVDADGFAILESSRSDAAVHTAIGHDTAVCPDCLAELFDPANRRYRYAFINCTQCGPRYTLTWALPYDRA
TTSMAPFPQCRPCLDEYNAPEHRRFHAEPNACPDCGPSLALLNAQGMPVEDVDPIAETVARLQRGEIVAIKGLGGFHLAC
DAHNADAVARLRSRKQREEKPFAVMVANLATAAQWGDIGSGEAALLTASERPIVLLRKRSGVDGRFAGVAPGLVWLGVML
PYTPLQYLLFHEAAGRPEGLGWLAQPQSLVLVMTSANPGGEPLVTGNDEAAQRLTGIADAFLLHDREILVRCDDSVVRGD
GEPAPHVQFIRRARGYTPRAIKLARSGPSVLALGGSFKNTVCLTRGDEAFVSQHVGDLGNAATCEALIEAVAHLQRVLEI
RPQLVAHDLHPDFFSTRHAAELAAQWGVPAVAVQHHHAHIAAVLAEHGSDEPAIGLALDGVGLGDDGQAWGGELLLVDGG
ACKRLGHLRELPLPGGDRAAREPWRMAAAALHAMGRGEEIEGRFPRQPGAPMVNRMLAQRLNAPLSSSMGRWFDAAAGLL
GTRETMAYEGQAAMLLEGLAESWGEQPSPGRPKTVAHSLGGVPRSGGGTYKALALPDAWRIDAGNTLDLLPLLEALSAET
NAARGAAQFHATLVAALEAWTVATVQVTGVRTVVFGGGCFLNHILARNLCRRLAARGLTVLTARQLPPNDGGIALGQVWV
ALQRAPN
>Mature_807_residues
MLMPRRPRNPRTVRIRIRVRGVVQGVGFRPFVYRLARELGLAGWVRNDGAGVDIEAQGSAAALVELRERLRRDAPPLARV
DEIGEERCAAQVDADGFAILESSRSDAAVHTAIGHDTAVCPDCLAELFDPANRRYRYAFINCTQCGPRYTLTWALPYDRA
TTSMAPFPQCRPCLDEYNAPEHRRFHAEPNACPDCGPSLALLNAQGMPVEDVDPIAETVARLQRGEIVAIKGLGGFHLAC
DAHNADAVARLRSRKQREEKPFAVMVANLATAAQWGDIGSGEAALLTASERPIVLLRKRSGVDGRFAGVAPGLVWLGVML
PYTPLQYLLFHEAAGRPEGLGWLAQPQSLVLVMTSANPGGEPLVTGNDEAAQRLTGIADAFLLHDREILVRCDDSVVRGD
GEPAPHVQFIRRARGYTPRAIKLARSGPSVLALGGSFKNTVCLTRGDEAFVSQHVGDLGNAATCEALIEAVAHLQRVLEI
RPQLVAHDLHPDFFSTRHAAELAAQWGVPAVAVQHHHAHIAAVLAEHGSDEPAIGLALDGVGLGDDGQAWGGELLLVDGG
ACKRLGHLRELPLPGGDRAAREPWRMAAAALHAMGRGEEIEGRFPRQPGAPMVNRMLAQRLNAPLSSSMGRWFDAAAGLL
GTRETMAYEGQAAMLLEGLAESWGEQPSPGRPKTVAHSLGGVPRSGGGTYKALALPDAWRIDAGNTLDLLPLLEALSAET
NAARGAAQFHATLVAALEAWTVATVQVTGVRTVVFGGGCFLNHILARNLCRRLAARGLTVLTARQLPPNDGGIALGQVWV
ALQRAPN

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain

Homologues:

Organism=Escherichia coli, GI2367152, Length=805, Percent_Identity=40.8695652173913, Blast_Score=451, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYPF2_CUPNH (O07451)

Other databases:

- EMBL:   AF002655
- EMBL:   AY305378
- RefSeq:   NP_942735.1
- ProteinModelPortal:   O07451
- SMR:   O07451
- GeneID:   2656550
- GenomeReviews:   AY305378_GR
- KEGG:   reh:PHG096
- HOGENOM:   HBG287964
- OMA:   NYPETAS
- PhylomeDB:   O07451
- ProtClustDB:   CLSK923813
- BioCyc:   REUT381666:PHG096-MONOMER
- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125
- Gene3D:   G3DSA:3.90.870.10
- PANTHER:   PTHR10029
- PIRSF:   PIRSF006256
- TIGRFAMs:   TIGR00143

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF; SSF54975 Acylphosphatase; SSF55821 DHBP_synth_RibB-like_a/b_dom

EC number: NA

Molecular weight: Translated: 86439; Mature: 86439

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS00150 ACYLPHOSPHATASE_1; PS00151 ACYLPHOSPHATASE_2; PS51160 ACYLPHOSPHATASE_3; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMPRRPRNPRTVRIRIRVRGVVQGVGFRPFVYRLARELGLAGWVRNDGAGVDIEAQGSA
CCCCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHCCCCEEECCCCCEEEECCCCH
AALVELRERLRRDAPPLARVDEIGEERCAAQVDADGFAILESSRSDAAVHTAIGHDTAVC
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEECCCCCCCC
PDCLAELFDPANRRYRYAFINCTQCGPRYTLTWALPYDRATTSMAPFPQCRPCLDEYNAP
HHHHHHHHCCCCCEEEEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCC
EHRRFHAEPNACPDCGPSLALLNAQGMPVEDVDPIAETVARLQRGEIVAIKGLGGFHLAC
CCCEECCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCEEEEE
DAHNADAVARLRSRKQREEKPFAVMVANLATAAQWGDIGSGEAALLTASERPIVLLRKRS
CCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECC
GVDGRFAGVAPGLVWLGVMLPYTPLQYLLFHEAAGRPEGLGWLAQPQSLVLVMTSANPGG
CCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCC
EPLVTGNDEAAQRLTGIADAFLLHDREILVRCDDSVVRGDGEPAPHVQFIRRARGYTPRA
CCEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCEECCCCCCCHHHHHHHHHCCCCCCE
IKLARSGPSVLALGGSFKNTVCLTRGDEAFVSQHVGDLGNAATCEALIEAVAHLQRVLEI
EEEECCCCCEEEECCCCCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RPQLVAHDLHPDFFSTRHAAELAAQWGVPAVAVQHHHAHIAAVLAEHGSDEPAIGLALDG
CHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEEEECC
VGLGDDGQAWGGELLLVDGGACKRLGHLRELPLPGGDRAAREPWRMAAAALHAMGRGEEI
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHCCCCCC
EGRFPRQPGAPMVNRMLAQRLNAPLSSSMGRWFDAAAGLLGTRETMAYEGQAAMLLEGLA
CCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHEECCCHHHHHHHHH
ESWGEQPSPGRPKTVAHSLGGVPRSGGGTYKALALPDAWRIDAGNTLDLLPLLEALSAET
HHHCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCEEECCCCCCHHHHHHHHHCCCC
NAARGAAQFHATLVAALEAWTVATVQVTGVRTVVFGGGCFLNHILARNLCRRLAARGLTV
HHHHHHHHHHHHHHHHHHHHEEEEEEEECEEEEEECCHHHHHHHHHHHHHHHHHHCCCEE
LTARQLPPNDGGIALGQVWVALQRAPN
EEECCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MLMPRRPRNPRTVRIRIRVRGVVQGVGFRPFVYRLARELGLAGWVRNDGAGVDIEAQGSA
CCCCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHCCCCEEECCCCCEEEECCCCH
AALVELRERLRRDAPPLARVDEIGEERCAAQVDADGFAILESSRSDAAVHTAIGHDTAVC
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEECCCCCCCC
PDCLAELFDPANRRYRYAFINCTQCGPRYTLTWALPYDRATTSMAPFPQCRPCLDEYNAP
HHHHHHHHCCCCCEEEEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCC
EHRRFHAEPNACPDCGPSLALLNAQGMPVEDVDPIAETVARLQRGEIVAIKGLGGFHLAC
CCCEECCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCEEEEE
DAHNADAVARLRSRKQREEKPFAVMVANLATAAQWGDIGSGEAALLTASERPIVLLRKRS
CCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECC
GVDGRFAGVAPGLVWLGVMLPYTPLQYLLFHEAAGRPEGLGWLAQPQSLVLVMTSANPGG
CCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCC
EPLVTGNDEAAQRLTGIADAFLLHDREILVRCDDSVVRGDGEPAPHVQFIRRARGYTPRA
CCEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCEECCCCCCCHHHHHHHHHCCCCCCE
IKLARSGPSVLALGGSFKNTVCLTRGDEAFVSQHVGDLGNAATCEALIEAVAHLQRVLEI
EEEECCCCCEEEECCCCCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RPQLVAHDLHPDFFSTRHAAELAAQWGVPAVAVQHHHAHIAAVLAEHGSDEPAIGLALDG
CHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEEEECC
VGLGDDGQAWGGELLLVDGGACKRLGHLRELPLPGGDRAAREPWRMAAAALHAMGRGEEI
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHCCCCCC
EGRFPRQPGAPMVNRMLAQRLNAPLSSSMGRWFDAAAGLLGTRETMAYEGQAAMLLEGLA
CCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHEECCCHHHHHHHHH
ESWGEQPSPGRPKTVAHSLGGVPRSGGGTYKALALPDAWRIDAGNTLDLLPLLEALSAET
HHHCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCEEECCCCCCHHHHHHHHHCCCC
NAARGAAQFHATLVAALEAWTVATVQVTGVRTVVFGGGCFLNHILARNLCRRLAARGLTV
HHHHHHHHHHHHHHHHHHHHEEEEEEEECEEEEEECCHHHHHHHHHHHHHHHHHHCCCEE
LTARQLPPNDGGIALGQVWVALQRAPN
EEECCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9799289; 12948488