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The map label for this gene is hslU

Identifier: 37681200

GI number: 37681200

Start: 3097452

End: 3098789

Strand: Direct

Name: hslU

Synonym: VV3016

Alternate gene names: 37681200

Gene position: 3097452-3098789 (Clockwise)

Preceding gene: 37681199

Following gene: 37681201

Centisome position: 92.34

GC content: 47.53

Gene sequence:

>1338_bases
ATGATTATGTCTGAAATGACTCCTCGTGAAATTGTCCATGAACTGAATCGACACATCATTGGTCAAGACAATGCAAAACG
CTCTGTGGCAATTGCACTACGTAATCGCTGGCGTCGAATGCAACTTGAAGAAAGCTTGCGCGTAGAAGTCACGCCAAAGA
ATATTCTGATGATCGGCCCAACTGGGGTTGGTAAAACCGAAATCGCTCGTCGTCTTGCGAAGTTGGCCAACGCACCTTTC
ATAAAAGTGGAAGCCACCAAATTCACCGAAGTGGGTTATGTGGGTAAAGAAGTGGAAAGCATTATTCGCGATCTGACTGA
CGTGGCAGTAAAGCTGACTCATCAGCAAGCGATGGAAAAAGTCAAATTCCGTGCAGAAGAGCTCGCGGAAGAACGAATCC
TTGATGCGCTCCTACCACCACCTCGCGATGCTTGGGGCCAGAACGAGCAAAGCGAAGATACCTCGAACACTCGCCAAATC
TTCCGTAAAAAACTGCGTGAAGGAAAGCTCGACGACAAAGAAATTGAAATCAATGTCGCAGCGCCACAGATGGGCGTGGA
AATCATGGCGCCTCCTGGTATGGAAGAGATGACTAACCAGCTACAAGGGATGTTCCAGAGCCTAGCTGGCAACACCAGTA
AAAAGCGCAAATTGAAAATCAAAGACGCCTTTAAAGCACTGATTGAAGAAGAAGCCGCTAAGCTGGTTAATCAAGATGAA
CTTAAAGAGCAAGCCATCTACAGTGTAGAAAACAATGGCATCGTGTTTATCGATGAGATCGATAAAATCTGTAAACGCGG
TGAGAGCTCAGGCCCGGATGTGTCTCGAGAAGGCGTTCAGCGTGACTTGTTGCCTCTTATCGAAGGCAGCACTGTCTCCA
CCAAGCACGGTATGGTAAAGACCGACCATATTCTGTTTATTGCCTCTGGTGCTTTCCAAGTCGCTAAGCCTTCTGACCTA
ATTCCTGAACTGCAAGGTCGATTGCCGATCCGTGTTGAGCTTGAGGCGCTTTCAAGCCACGATTTCAAGCGTATTCTGAC
CGAGCCAAGAGCATCACTGACAGAACAATATGTCGCTCTGATGAAAACAGAAGATGTGGACATCGAATTTACGGAAGACG
GCATCACGCAAATTGCAGAAGCCGCCTGGACCGTGAATGAAACCACAGAGAACATTGGTGCTCGCCGTCTACATACCGTA
ATGGAACGCCTGATGGATGAGATCTCATTCGATGCAACGGAAAAATCAGGCACCAAGTTTGTGATTGATGCAGCGTACGT
GCAGCAACGTCTCGGCGAATTTGTTGAAGATGAAGACTTAAGCCGTTTCATCCTGTAA

Upstream 100 bases:

>100_bases
TTGTGTGTTTACCAACCACAACCACACCATTGAAGAGCTCGAGATCCCAGCAGAATTGCCAAATCTCAGCCAAGCTTAAT
TCGCCCACAGATTGTAAGGA

Downstream 100 bases:

>100_bases
TCCTCTTGAGTAGCAAGGGCGAGAGCATTATTTCTCTCGCCCTTTTTCTTCCTCTTCTCACCTAGCGCCTTTGCATTTAG
CCGTATTGCGCTCCCTTAAG

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MIMSEMTPREIVHELNRHIIGQDNAKRSVAIALRNRWRRMQLEESLRVEVTPKNILMIGPTGVGKTEIARRLAKLANAPF
IKVEATKFTEVGYVGKEVESIIRDLTDVAVKLTHQQAMEKVKFRAEELAEERILDALLPPPRDAWGQNEQSEDTSNTRQI
FRKKLREGKLDDKEIEINVAAPQMGVEIMAPPGMEEMTNQLQGMFQSLAGNTSKKRKLKIKDAFKALIEEEAAKLVNQDE
LKEQAIYSVENNGIVFIDEIDKICKRGESSGPDVSREGVQRDLLPLIEGSTVSTKHGMVKTDHILFIASGAFQVAKPSDL
IPELQGRLPIRVELEALSSHDFKRILTEPRASLTEQYVALMKTEDVDIEFTEDGITQIAEAAWTVNETTENIGARRLHTV
MERLMDEISFDATEKSGTKFVIDAAYVQQRLGEFVEDEDLSRFIL

Sequences:

>Translated_445_residues
MIMSEMTPREIVHELNRHIIGQDNAKRSVAIALRNRWRRMQLEESLRVEVTPKNILMIGPTGVGKTEIARRLAKLANAPF
IKVEATKFTEVGYVGKEVESIIRDLTDVAVKLTHQQAMEKVKFRAEELAEERILDALLPPPRDAWGQNEQSEDTSNTRQI
FRKKLREGKLDDKEIEINVAAPQMGVEIMAPPGMEEMTNQLQGMFQSLAGNTSKKRKLKIKDAFKALIEEEAAKLVNQDE
LKEQAIYSVENNGIVFIDEIDKICKRGESSGPDVSREGVQRDLLPLIEGSTVSTKHGMVKTDHILFIASGAFQVAKPSDL
IPELQGRLPIRVELEALSSHDFKRILTEPRASLTEQYVALMKTEDVDIEFTEDGITQIAEAAWTVNETTENIGARRLHTV
MERLMDEISFDATEKSGTKFVIDAAYVQQRLGEFVEDEDLSRFIL
>Mature_445_residues
MIMSEMTPREIVHELNRHIIGQDNAKRSVAIALRNRWRRMQLEESLRVEVTPKNILMIGPTGVGKTEIARRLAKLANAPF
IKVEATKFTEVGYVGKEVESIIRDLTDVAVKLTHQQAMEKVKFRAEELAEERILDALLPPPRDAWGQNEQSEDTSNTRQI
FRKKLREGKLDDKEIEINVAAPQMGVEIMAPPGMEEMTNQLQGMFQSLAGNTSKKRKLKIKDAFKALIEEEAAKLVNQDE
LKEQAIYSVENNGIVFIDEIDKICKRGESSGPDVSREGVQRDLLPLIEGSTVSTKHGMVKTDHILFIASGAFQVAKPSDL
IPELQGRLPIRVELEALSSHDFKRILTEPRASLTEQYVALMKTEDVDIEFTEDGITQIAEAAWTVNETTENIGARRLHTV
MERLMDEISFDATEKSGTKFVIDAAYVQQRLGEFVEDEDLSRFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790366, Length=443, Percent_Identity=79.2325056433409, Blast_Score=716, Evalue=0.0,
Organism=Escherichia coli, GI1786642, Length=100, Percent_Identity=47, Blast_Score=96, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6319704, Length=122, Percent_Identity=33.6065573770492, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648289, Length=272, Percent_Identity=30.1470588235294, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648291, Length=272, Percent_Identity=30.1470588235294, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 50238; Mature: 50238

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMSEMTPREIVHELNRHIIGQDNAKRSVAIALRNRWRRMQLEESLRVEVTPKNILMIGP
CCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECC
TGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVESIIRDLTDVAVKLTHQQAMEK
CCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKFRAEELAEERILDALLPPPRDAWGQNEQSEDTSNTRQIFRKKLREGKLDDKEIEINVA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEE
APQMGVEIMAPPGMEEMTNQLQGMFQSLAGNTSKKRKLKIKDAFKALIEEEAAKLVNQDE
CCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHEEHHHHHHHHHHHHHHHHHHCHHH
LKEQAIYSVENNGIVFIDEIDKICKRGESSGPDVSREGVQRDLLPLIEGSTVSTKHGMVK
HHHHHHHEECCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEE
TDHILFIASGAFQVAKPSDLIPELQGRLPIRVELEALSSHDFKRILTEPRASLTEQYVAL
ECCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEHHHHCCCHHHHHHHCHHHHHHHHHHHH
MKTEDVDIEFTEDGITQIAEAAWTVNETTENIGARRLHTVMERLMDEISFDATEKSGTKF
HHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
VIDAAYVQQRLGEFVEDEDLSRFIL
EEEHHHHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure
MIMSEMTPREIVHELNRHIIGQDNAKRSVAIALRNRWRRMQLEESLRVEVTPKNILMIGP
CCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECC
TGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVESIIRDLTDVAVKLTHQQAMEK
CCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKFRAEELAEERILDALLPPPRDAWGQNEQSEDTSNTRQIFRKKLREGKLDDKEIEINVA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEE
APQMGVEIMAPPGMEEMTNQLQGMFQSLAGNTSKKRKLKIKDAFKALIEEEAAKLVNQDE
CCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHEEHHHHHHHHHHHHHHHHHHCHHH
LKEQAIYSVENNGIVFIDEIDKICKRGESSGPDVSREGVQRDLLPLIEGSTVSTKHGMVK
HHHHHHHEECCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEE
TDHILFIASGAFQVAKPSDLIPELQGRLPIRVELEALSSHDFKRILTEPRASLTEQYVAL
ECCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEHHHHCCCHHHHHHHCHHHHHHHHHHHH
MKTEDVDIEFTEDGITQIAEAAWTVNETTENIGARRLHTVMERLMDEISFDATEKSGTKF
HHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
VIDAAYVQQRLGEFVEDEDLSRFIL
EEEHHHHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA