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The map label for this gene is metK [H]

Identifier: 37681047

GI number: 37681047

Start: 2919395

End: 2920549

Strand: Direct

Name: metK [H]

Synonym: VV2863

Alternate gene names: 37681047

Gene position: 2919395-2920549 (Clockwise)

Preceding gene: 37681045

Following gene: 37681048

Centisome position: 87.03

GC content: 47.19

Gene sequence:

>1155_bases
ATGGCTAAGCACCTGTTTACTTCTGAGTCGGTTTCAGAAGGTCATCCAGATAAAATTGCAGACCAGATTTCTGATGCGGT
TCTTGATGCGATTCTTGAACAAGACCCAAAAGCGCGTGTTGCATGTGAAACGTACGTAAAAACTGGCATGGTAATGGTTG
GTGGTGAAATCACCACTTCAGCATGGGTTGATATCGAAGAGTTAACTCGTGAAACGGTGCGTGAAATTGGTTATGTACAT
TCAGATATGGGTTTCGATGCAAACTCATGTGCAGTACTGAACACTATCGGTAAACAGTCTCCAGACATCAACCAAGGTGT
TGATAAAGCCGATCCTAAAGAGCAAGGCGCGGGCGACCAAGGCATCATGTTCGGTTACGCATGTAACGAAACTGAAGTCC
TTATGCCAGCACCAATCACTTACGCTCACCGTTTGATGGAGCGTCAAGCGAAAGTTCGTAAAGATGGCACACTGCCATGG
TTGCGTCCTGATGCAAAATCACAGGTTACTTTCCAATACGAGCAAGGCAAAATTGTTGGCATTGATGCGGTTGTTCTTTC
AACTCAGCACTGCGATTCTATTTCCACACCGGATCTTCGCGAAGCGGTTATGGAAGAGATCATCAAGCCAGTTCTTCCTT
CTGAATGGCTAAACAAAGAAACAAAATACTTCATCAACCCAACTGGCCGTTTCGTTATCGGTGGTCCAATGGGTGACTGT
GGTTTGACTGGTCGTAAGATCATCGTTGATACCTACGGCGGCGCAGCTCGTCACGGTGGTGGTGCTTTCTCTGGTAAAGA
TCCATCGAAAGTAGACCGTTCAGCTGCTTACGCAGCACGTTACGTAGCGAAAAACATCGTCGCGGCGGGTATGGCAGATC
GCTGTGAAATCCAACTGTCTTACGCAATCGGTGTTGCTGATCCAACCTCTATCATGGTGGAAACATTCGGTACTGAGAAA
GTATCACACGACATCATCATTGAAGCGGTACGTCAGTTCTTCGACCTACGTCCATACGGTCTACAAGAGATGCTGAATCT
ACTTCAGCCAATCTACAAGAAGACCGCAGCATACGGTCACTTCGGTCGCGAAGAGTTCCCATGGGAAGCGACTGACAAAG
CAGCACTACTGCGCGAGTTTGCTGGTATCAAGTAA

Upstream 100 bases:

>100_bases
TAGACGTCTAGATGTAGAAACACCTACAAAAATTATTGTATTTCCTACTGCTGGAGCATGCATTCTCACAGCAGAATAGA
TTAATAAAAGTGGAGCTCTC

Downstream 100 bases:

>100_bases
GATAACCTCACCTCATTTTCTAAAGCCTTCGCTCAATGCGAAGGCTTTTTCATTTGTGCGACGAAAAATTCGCCACGGCA
TTTGTATTTTTAACTAACTC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEITTSAWVDIEELTRETVREIGYVH
SDMGFDANSCAVLNTIGKQSPDINQGVDKADPKEQGAGDQGIMFGYACNETEVLMPAPITYAHRLMERQAKVRKDGTLPW
LRPDAKSQVTFQYEQGKIVGIDAVVLSTQHCDSISTPDLREAVMEEIIKPVLPSEWLNKETKYFINPTGRFVIGGPMGDC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGMADRCEIQLSYAIGVADPTSIMVETFGTEK
VSHDIIIEAVRQFFDLRPYGLQEMLNLLQPIYKKTAAYGHFGREEFPWEATDKAALLREFAGIK

Sequences:

>Translated_384_residues
MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEITTSAWVDIEELTRETVREIGYVH
SDMGFDANSCAVLNTIGKQSPDINQGVDKADPKEQGAGDQGIMFGYACNETEVLMPAPITYAHRLMERQAKVRKDGTLPW
LRPDAKSQVTFQYEQGKIVGIDAVVLSTQHCDSISTPDLREAVMEEIIKPVLPSEWLNKETKYFINPTGRFVIGGPMGDC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGMADRCEIQLSYAIGVADPTSIMVETFGTEK
VSHDIIIEAVRQFFDLRPYGLQEMLNLLQPIYKKTAAYGHFGREEFPWEATDKAALLREFAGIK
>Mature_383_residues
AKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEITTSAWVDIEELTRETVREIGYVHS
DMGFDANSCAVLNTIGKQSPDINQGVDKADPKEQGAGDQGIMFGYACNETEVLMPAPITYAHRLMERQAKVRKDGTLPWL
RPDAKSQVTFQYEQGKIVGIDAVVLSTQHCDSISTPDLREAVMEEIIKPVLPSEWLNKETKYFINPTGRFVIGGPMGDCG
LTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGMADRCEIQLSYAIGVADPTSIMVETFGTEKV
SHDIIIEAVRQFFDLRPYGLQEMLNLLQPIYKKTAAYGHFGREEFPWEATDKAALLREFAGIK

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=374, Percent_Identity=56.1497326203209, Blast_Score=410, Evalue=1e-115,
Organism=Homo sapiens, GI4557737, Length=374, Percent_Identity=56.4171122994652, Blast_Score=408, Evalue=1e-114,
Organism=Escherichia coli, GI1789311, Length=384, Percent_Identity=85.9375, Blast_Score=695, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17551082, Length=373, Percent_Identity=55.2278820375335, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17538498, Length=373, Percent_Identity=56.8364611260054, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17538494, Length=373, Percent_Identity=56.5683646112601, Blast_Score=401, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25145633, Length=384, Percent_Identity=54.9479166666667, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI32565905, Length=373, Percent_Identity=50.6702412868633, Blast_Score=338, Evalue=2e-93,
Organism=Caenorhabditis elegans, GI32565911, Length=326, Percent_Identity=53.3742331288344, Blast_Score=328, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI25145635, Length=337, Percent_Identity=51.3353115727003, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI32565903, Length=299, Percent_Identity=52.8428093645485, Blast_Score=295, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=65.0602409638554, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32565907, Length=46, Percent_Identity=73.9130434782609, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320710, Length=374, Percent_Identity=58.8235294117647, Blast_Score=420, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6323209, Length=370, Percent_Identity=58.3783783783784, Blast_Score=413, Evalue=1e-116,
Organism=Drosophila melanogaster, GI45552159, Length=373, Percent_Identity=55.7640750670241, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580529, Length=373, Percent_Identity=55.7640750670241, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580537, Length=373, Percent_Identity=55.7640750670241, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580533, Length=373, Percent_Identity=55.7640750670241, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580535, Length=373, Percent_Identity=55.7640750670241, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580531, Length=373, Percent_Identity=55.7640750670241, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580527, Length=373, Percent_Identity=55.2278820375335, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580525, Length=373, Percent_Identity=55.2278820375335, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580541, Length=372, Percent_Identity=52.9569892473118, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580539, Length=120, Percent_Identity=56.6666666666667, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42092; Mature: 41961

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEITTS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCEECCH
AWVDIEELTRETVREIGYVHSDMGFDANSCAVLNTIGKQSPDINQGVDKADPKEQGAGDQ
HCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHCCCCCCCCHHHCCCCC
GIMFGYACNETEVLMPAPITYAHRLMERQAKVRKDGTLPWLRPDAKSQVTFQYEQGKIVG
CEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEE
IDAVVLSTQHCDSISTPDLREAVMEEIIKPVLPSEWLNKETKYFINPTGRFVIGGPMGDC
EEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGMADRCEIQLS
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGVADPTSIMVETFGTEKVSHDIIIEAVRQFFDLRPYGLQEMLNLLQPIYKKTAAYGH
EEEECCCCHHHHEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
FGREEFPWEATDKAALLREFAGIK
CCCCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
AKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEITTS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCEECCH
AWVDIEELTRETVREIGYVHSDMGFDANSCAVLNTIGKQSPDINQGVDKADPKEQGAGDQ
HCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHCCCCCCCCHHHCCCCC
GIMFGYACNETEVLMPAPITYAHRLMERQAKVRKDGTLPWLRPDAKSQVTFQYEQGKIVG
CEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEE
IDAVVLSTQHCDSISTPDLREAVMEEIIKPVLPSEWLNKETKYFINPTGRFVIGGPMGDC
EEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGMADRCEIQLS
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGVADPTSIMVETFGTEKVSHDIIIEAVRQFFDLRPYGLQEMLNLLQPIYKKTAAYGH
EEEECCCCHHHHEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
FGREEFPWEATDKAALLREFAGIK
CCCCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA