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The map label for this gene is potB

Identifier: 37679870

GI number: 37679870

Start: 1739598

End: 1740455

Strand: Direct

Name: potB

Synonym: VV1686

Alternate gene names: 37679870

Gene position: 1739598-1740455 (Clockwise)

Preceding gene: 37679869

Following gene: 37679871

Centisome position: 51.86

GC content: 44.87

Gene sequence:

>858_bases
ATGAGCAAGAAATTTAGTCTTCAAAACGCCATTATCACGCTGATTGTTGGTTGGTTGGTTCTTTTTGTTCTTATTCCAAA
CCTGATGATCATTGGCACCAGTTTTCTGACGCGTGATGAAGCCAACCTCATTGAAATGACGTTTACATTGGATAACTACG
CGCGTTTGCTCGATCCTTTGTACGTGAAAGTGTTGCTGCACTCCTTCTACATGGCGATTGTTGCAACCCTTATCTGTTTG
GTTGTGGGCTACCCATTTGCTTACATTGTTGCGAAGATGCCACACAAATGGCGTCCATTTATGCTGTTTTTGGTGATTGT
GCCTTTCTGGACCAACTCCCTGATTCGTACCTACGGCCTAAAAATCGTATTGGGTACGCAAGGCATTCTCAATAAAGCGC
TATTGAGCATTGGTCTTATTGATACGCCTATGCGCATTATGTTTACCGAAACCGCGGTTATGATTGGTTTAGTGTACATT
TTGCTGCCATTCATGATTTTGCCGTTGTACTCAGCCATTGAAAAGCTGGACGACACGTATATCGAAGCGGCAAAAGACTT
AGGTGCAAATAAGTTCCAGACGCTAACAAAGGTCATTCTGCCATTGACAATGCCTGGCATTATCGGCGGTTGTTTGTTAG
TTCTCCTCCCTGCTCTGGGCATGTTCTACATTTCAGACTTGCTTGGCGGTGCGAAAAACTTGCTGATCGGTAACGTCATT
AAGAGCCAAGTGCTCAATGCACGTGATTGGCCATTTGGGGCTGCGACCAGTATCGCGCTGACCATCGCAATGGCGATTAT
GCTTTATGCCTACTATCGCGCGGGTAAATTGTTGAATAAGAAAGTGGAGCTGGACTAA

Upstream 100 bases:

>100_bases
GTAAGCGAGTTCTTTAACGAAGATGATCCTGATGTGGACCACTCTTTGGGACAAAAAGTCGCCATTACTTGGGTTGAAAG
CTGGGAAGTGGTGCTAAGCG

Downstream 100 bases:

>100_bases
TGGGGCGTACAGTTAGATTTAGCTTTATGACGTTGGTTTATGCGTTTTTGTATTTACCAATCATCGTATTGATCGCCAAC
TCGTTTAACGAAAACAAGTT

Product: spermidine/putrescine ABC transporter membrane protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSKKFSLQNAIITLIVGWLVLFVLIPNLMIIGTSFLTRDEANLIEMTFTLDNYARLLDPLYVKVLLHSFYMAIVATLICL
VVGYPFAYIVAKMPHKWRPFMLFLVIVPFWTNSLIRTYGLKIVLGTQGILNKALLSIGLIDTPMRIMFTETAVMIGLVYI
LLPFMILPLYSAIEKLDDTYIEAAKDLGANKFQTLTKVILPLTMPGIIGGCLLVLLPALGMFYISDLLGGAKNLLIGNVI
KSQVLNARDWPFGAATSIALTIAMAIMLYAYYRAGKLLNKKVELD

Sequences:

>Translated_285_residues
MSKKFSLQNAIITLIVGWLVLFVLIPNLMIIGTSFLTRDEANLIEMTFTLDNYARLLDPLYVKVLLHSFYMAIVATLICL
VVGYPFAYIVAKMPHKWRPFMLFLVIVPFWTNSLIRTYGLKIVLGTQGILNKALLSIGLIDTPMRIMFTETAVMIGLVYI
LLPFMILPLYSAIEKLDDTYIEAAKDLGANKFQTLTKVILPLTMPGIIGGCLLVLLPALGMFYISDLLGGAKNLLIGNVI
KSQVLNARDWPFGAATSIALTIAMAIMLYAYYRAGKLLNKKVELD
>Mature_284_residues
SKKFSLQNAIITLIVGWLVLFVLIPNLMIIGTSFLTRDEANLIEMTFTLDNYARLLDPLYVKVLLHSFYMAIVATLICLV
VGYPFAYIVAKMPHKWRPFMLFLVIVPFWTNSLIRTYGLKIVLGTQGILNKALLSIGLIDTPMRIMFTETAVMIGLVYIL
LPFMILPLYSAIEKLDDTYIEAAKDLGANKFQTLTKVILPLTMPGIIGGCLLVLLPALGMFYISDLLGGAKNLLIGNVIK
SQVLNARDWPFGAATSIALTIAMAIMLYAYYRAGKLLNKKVELD

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=275, Percent_Identity=72.3636363636364, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI1787080, Length=234, Percent_Identity=41.025641025641, Blast_Score=185, Evalue=3e-48,
Organism=Escherichia coli, GI1787713, Length=265, Percent_Identity=28.3018867924528, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31764; Mature: 31633

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKFSLQNAIITLIVGWLVLFVLIPNLMIIGTSFLTRDEANLIEMTFTLDNYARLLDPL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHH
YVKVLLHSFYMAIVATLICLVVGYPFAYIVAKMPHKWRPFMLFLVIVPFWTNSLIRTYGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
KIVLGTQGILNKALLSIGLIDTPMRIMFTETAVMIGLVYILLPFMILPLYSAIEKLDDTY
EEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEAAKDLGANKFQTLTKVILPLTMPGIIGGCLLVLLPALGMFYISDLLGGAKNLLIGNVI
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
KSQVLNARDWPFGAATSIALTIAMAIMLYAYYRAGKLLNKKVELD
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SKKFSLQNAIITLIVGWLVLFVLIPNLMIIGTSFLTRDEANLIEMTFTLDNYARLLDPL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHH
YVKVLLHSFYMAIVATLICLVVGYPFAYIVAKMPHKWRPFMLFLVIVPFWTNSLIRTYGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
KIVLGTQGILNKALLSIGLIDTPMRIMFTETAVMIGLVYILLPFMILPLYSAIEKLDDTY
EEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEAAKDLGANKFQTLTKVILPLTMPGIIGGCLLVLLPALGMFYISDLLGGAKNLLIGNVI
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
KSQVLNARDWPFGAATSIALTIAMAIMLYAYYRAGKLLNKKVELD
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]