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The map label for this gene is mii [H]

Identifier: 37679716

GI number: 37679716

Start: 1570823

End: 1572061

Strand: Direct

Name: mii [H]

Synonym: VV1532

Alternate gene names: 37679716

Gene position: 1570823-1572061 (Clockwise)

Preceding gene: 37679715

Following gene: 37679717

Centisome position: 46.83

GC content: 53.11

Gene sequence:

>1239_bases
ATGAAACCTTCTACACAATCCTCTACGCATAGCTCCACCAAAAGCTCCCCGCAACGCGATGCGCGGATGAGCCAAATGAA
AGTGCCTGCCACGTATATGCGAGGCGGCACCAGCAAGGGGGTGTTTTTCAACCTTGCGGATTTACCGCCAGAGGCACAAA
TTGCGGGGGAAAAACGTGATCAACTGCTGCTGCGCGTGATCGGCAGCCCAGATCCTTACGGCAAACAGATTGACGGCATG
GGCGGGGCGACATCCAGCACCAGCAAAACGGTGATTGTGAGCAAAAGTAGCCGCGCCGATCACGATGTTGATTACCTGTT
TGGTCAAGTCTCTATCGACAAACCGTTTGTCGATTGGAGCGGTAACTGTGGCAACTTGTCTGCTGCGGTTGGCCCATTTG
CCATCCATGCTGGCTTGATTGACGAAAAACGCATTCCTCAAAATGGCGTGGTGACGGTGAAAGTGTGGCAAGCCAACATT
GGCAAAACCATTGAAGTGCGCGTGCCCATTCAACATGGTTTGGTGCAGGAAACGGGCGAGTTTTCTCTTGATGGCGTGAC
ATTTCCTGCTGCAGAGATCCAAGTGGATTTCGTTGACCCAGCCGATGGCGAGGGGAGCATGTTTCCCACTGGCAATTTGA
TGGATGATCTTGTCGTACCGGGTATTGGCACCTTTAACGCCACGCTCATTAATGCGGGCATTCCCACCATTTTCCTTGAT
GCTGAGGCGTTGGGGTATTTGGGGACGGAACTGCAAGAGCAGATCAATAATGATGAGCAAGCGTTAGCCAAGTTTGAAAC
CATTCGTGCTTATGGCGCGCTAAAAATGGGTTTGATTAGTTCGATTGACGAAGCAGAAGGTCGCCAGCACACCCCAAAAG
TGGCGTTTGTCGCGAAGCCGAAAAGTTACCAAGCCTCAAGTGGCAAAGTGGTGGAGGCAGGAGAGGTTGATCTGCTGGTT
CGCGCCCTTTCAATGGGCAAGCTGCATCACGCCATGATGGGGACCGCAGCGGTCGCGATTGGCGCAGCCGCTTGCGTGCC
GGGCACTTTAGTGAACTTGGCTGCCGGTGGTGGCGAGAAACAAGCCGTCACCTTTGGCCATCCGTCTGGAACGCTCAAAG
TCGGTGCTCAAGCAAAAGCCACTGAACAAGGTTGGGTGGTAGAAAAAGCCATTATGAGCCGCAGTGCTCGCATACTGATG
GAGGGCTTTGTGCGTGTTCCCTCCGATCTCTTTGAATAA

Upstream 100 bases:

>100_bases
CGTGTCGTCTTGATACCGAAGATGAAGTGCATGTGTATCAAGCTGGCGGTGTGTTGCAGCGATTCGCGCAAGACTTTCTC
TCCCAGCAAGGAGCGTAACG

Downstream 100 bases:

>100_bases
AACTGTGTTTGATGACTTGTTCAAAGAGCAAGGTGAAAAGGACCGTTAAGACATACTGGAGGAAGCACTATGTCTGCTTA
TCAGAAAGAATATCAATGGG

Product: hypothetical protein

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MKPSTQSSTHSSTKSSPQRDARMSQMKVPATYMRGGTSKGVFFNLADLPPEAQIAGEKRDQLLLRVIGSPDPYGKQIDGM
GGATSSTSKTVIVSKSSRADHDVDYLFGQVSIDKPFVDWSGNCGNLSAAVGPFAIHAGLIDEKRIPQNGVVTVKVWQANI
GKTIEVRVPIQHGLVQETGEFSLDGVTFPAAEIQVDFVDPADGEGSMFPTGNLMDDLVVPGIGTFNATLINAGIPTIFLD
AEALGYLGTELQEQINNDEQALAKFETIRAYGALKMGLISSIDEAEGRQHTPKVAFVAKPKSYQASSGKVVEAGEVDLLV
RALSMGKLHHAMMGTAAVAIGAAACVPGTLVNLAAGGGEKQAVTFGHPSGTLKVGAQAKATEQGWVVEKAIMSRSARILM
EGFVRVPSDLFE

Sequences:

>Translated_412_residues
MKPSTQSSTHSSTKSSPQRDARMSQMKVPATYMRGGTSKGVFFNLADLPPEAQIAGEKRDQLLLRVIGSPDPYGKQIDGM
GGATSSTSKTVIVSKSSRADHDVDYLFGQVSIDKPFVDWSGNCGNLSAAVGPFAIHAGLIDEKRIPQNGVVTVKVWQANI
GKTIEVRVPIQHGLVQETGEFSLDGVTFPAAEIQVDFVDPADGEGSMFPTGNLMDDLVVPGIGTFNATLINAGIPTIFLD
AEALGYLGTELQEQINNDEQALAKFETIRAYGALKMGLISSIDEAEGRQHTPKVAFVAKPKSYQASSGKVVEAGEVDLLV
RALSMGKLHHAMMGTAAVAIGAAACVPGTLVNLAAGGGEKQAVTFGHPSGTLKVGAQAKATEQGWVVEKAIMSRSARILM
EGFVRVPSDLFE
>Mature_412_residues
MKPSTQSSTHSSTKSSPQRDARMSQMKVPATYMRGGTSKGVFFNLADLPPEAQIAGEKRDQLLLRVIGSPDPYGKQIDGM
GGATSSTSKTVIVSKSSRADHDVDYLFGQVSIDKPFVDWSGNCGNLSAAVGPFAIHAGLIDEKRIPQNGVVTVKVWQANI
GKTIEVRVPIQHGLVQETGEFSLDGVTFPAAEIQVDFVDPADGEGSMFPTGNLMDDLVVPGIGTFNATLINAGIPTIFLD
AEALGYLGTELQEQINNDEQALAKFETIRAYGALKMGLISSIDEAEGRQHTPKVAFVAKPKSYQASSGKVVEAGEVDLLV
RALSMGKLHHAMMGTAAVAIGAAACVPGTLVNLAAGGGEKQAVTFGHPSGTLKVGAQAKATEQGWVVEKAIMSRSARILM
EGFVRVPSDLFE

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=383, Percent_Identity=32.8981723237598, Blast_Score=162, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 43501; Mature: 43501

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSTQSSTHSSTKSSPQRDARMSQMKVPATYMRGGTSKGVFFNLADLPPEAQIAGEKRD
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHCCCCCCCEEEEECCCCCCCHHCCCCHH
QLLLRVIGSPDPYGKQIDGMGGATSSTSKTVIVSKSSRADHDVDYLFGQVSIDKPFVDWS
EEEHHEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHEEEEEEECCCCCCCC
GNCGNLSAAVGPFAIHAGLIDEKRIPQNGVVTVKVWQANIGKTIEVRVPIQHGLVQETGE
CCCCCCCHHHCCHHEEECCCCCCCCCCCCEEEEEEEECCCCCEEEEEECHHHCCHHCCCC
FSLDGVTFPAAEIQVDFVDPADGEGSMFPTGNLMDDLVVPGIGTFNATLINAGIPTIFLD
EEECCEECCCEEEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEECCCCEEEEC
AEALGYLGTELQEQINNDEQALAKFETIRAYGALKMGLISSIDEAEGRQHTPKVAFVAKP
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECC
KSYQASSGKVVEAGEVDLLVRALSMGKLHHAMMGTAAVAIGAAACVPGTLVNLAAGGGEK
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCC
QAVTFGHPSGTLKVGAQAKATEQGWVVEKAIMSRSARILMEGFVRVPSDLFE
CEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCC
>Mature Secondary Structure
MKPSTQSSTHSSTKSSPQRDARMSQMKVPATYMRGGTSKGVFFNLADLPPEAQIAGEKRD
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHCCCCCCCEEEEECCCCCCCHHCCCCHH
QLLLRVIGSPDPYGKQIDGMGGATSSTSKTVIVSKSSRADHDVDYLFGQVSIDKPFVDWS
EEEHHEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHEEEEEEECCCCCCCC
GNCGNLSAAVGPFAIHAGLIDEKRIPQNGVVTVKVWQANIGKTIEVRVPIQHGLVQETGE
CCCCCCCHHHCCHHEEECCCCCCCCCCCCEEEEEEEECCCCCEEEEEECHHHCCHHCCCC
FSLDGVTFPAAEIQVDFVDPADGEGSMFPTGNLMDDLVVPGIGTFNATLINAGIPTIFLD
EEECCEECCCEEEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEECCCCEEEEC
AEALGYLGTELQEQINNDEQALAKFETIRAYGALKMGLISSIDEAEGRQHTPKVAFVAKP
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECC
KSYQASSGKVVEAGEVDLLVRALSMGKLHHAMMGTAAVAIGAAACVPGTLVNLAAGGGEK
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCC
QAVTFGHPSGTLKVGAQAKATEQGWVVEKAIMSRSARILMEGFVRVPSDLFE
CEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA