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The map label for this gene is hisF1

Identifier: 37679540

GI number: 37679540

Start: 1381603

End: 1382376

Strand: Direct

Name: hisF1

Synonym: VV1356

Alternate gene names: 37679540

Gene position: 1381603-1382376 (Clockwise)

Preceding gene: 37679539

Following gene: 37679541

Centisome position: 41.19

GC content: 48.19

Gene sequence:

>774_bases
ATGCTGGCAAAGCGAATAATTCCATGTCTTGATGTTCGTGATGGACAAGTGGTGAAGGGCGTTCAGTTTCGTAACCATGA
AATCATTGGTGATATCGTTCCTCTTGCTAAACGCTATGCGGAAGAAGGCGCTGACGAGCTTGTATTCTACGATATTACCG
CCTCAAGTGATGGTCGTGTCGTCGATAAAAGTTGGGTTGCTCGCGTTGCAGAAGTGATTGATATTCCGTTCTGTGTCGCT
GGTGGCATTAAGTCAGCAGAAGACGCAGCGCGCATTCTTGAGTTTGGCGCAGACAAAGTCTCTATCAACTCGCCAGCGCT
GGCTAACCCGCAGCTGATTACTGACCTTGCCGATAAGTTTGGTGTGCAATGTATCGTCGTTGGTATCGATTCATACTACG
ACAAAGAGACCGGTAAGTATCAGGTTTACCAGTTTACTGGTGATGAAGAACGCACCAAAGCGACCCAATGGGAAACGCGT
GATTGGGTGCAAGAAGTACAAAAGCGCGGCGCTGGTGAAATCGTGCTGAACATGATGAACCAAGACGGCGTGCGTAACGG
CTACGACATCGAACAACTGAATATGGTGCGTGAAGTGTGTAACGTGCCATTGATTGCATCGGGCGGTGCAGGTGCCATGG
AGCATTTCGCAGAAGCCTACCAAAAAGCAAATGTAGATGGCGCGCTTGCCGCATCGGTATTCCACAAGCAAGTCATCAAT
ATTGGTGAACTAAAGCAGTATTTGAAACAACAAGGCATTGAGGTACGACTATGA

Upstream 100 bases:

>100_bases
CATTAGCGGATATCGAAGCACTAAAAGGCAGCGGTGTGGCTGGCGTGATTGTTGGTCGTGCACTGTTAGATGGCAAGTTC
ACAGCAGAGGAGGCATTTGC

Downstream 100 bases:

>100_bases
GTGTGAAAGCCGCCGAAGTAAGTTCGCTCGCTGAGCGAATCAACTGGGAAAAGGTGGATGGTCTGGTACCTGCCATCGTA
CAAGATTTCCAATCAAGCCA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF1; ImGP synthase subunit hisF1; IGPS subunit hisF1

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRVVDKSWVARVAEVIDIPFCVA
GGIKSAEDAARILEFGADKVSINSPALANPQLITDLADKFGVQCIVVGIDSYYDKETGKYQVYQFTGDEERTKATQWETR
DWVQEVQKRGAGEIVLNMMNQDGVRNGYDIEQLNMVREVCNVPLIASGGAGAMEHFAEAYQKANVDGALAASVFHKQVIN
IGELKQYLKQQGIEVRL

Sequences:

>Translated_257_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRVVDKSWVARVAEVIDIPFCVA
GGIKSAEDAARILEFGADKVSINSPALANPQLITDLADKFGVQCIVVGIDSYYDKETGKYQVYQFTGDEERTKATQWETR
DWVQEVQKRGAGEIVLNMMNQDGVRNGYDIEQLNMVREVCNVPLIASGGAGAMEHFAEAYQKANVDGALAASVFHKQVIN
IGELKQYLKQQGIEVRL
>Mature_257_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRVVDKSWVARVAEVIDIPFCVA
GGIKSAEDAARILEFGADKVSINSPALANPQLITDLADKFGVQCIVVGIDSYYDKETGKYQVYQFTGDEERTKATQWETR
DWVQEVQKRGAGEIVLNMMNQDGVRNGYDIEQLNMVREVCNVPLIASGGAGAMEHFAEAYQKANVDGALAASVFHKQVIN
IGELKQYLKQQGIEVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=85.9922178988327, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI87082028, Length=219, Percent_Identity=26.9406392694064, Blast_Score=72, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319725, Length=317, Percent_Identity=31.2302839116719, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS61_VIBVY (Q7MLS1)

Other databases:

- EMBL:   BA000037
- RefSeq:   NP_934149.1
- ProteinModelPortal:   Q7MLS1
- SMR:   Q7MLS1
- STRING:   Q7MLS1
- GeneID:   2624160
- GenomeReviews:   BA000037_GR
- KEGG:   vvy:VV1356
- NMPDR:   fig|196600.1.peg.1424
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   ASVFHKG
- ProtClustDB:   PRK02083
- BioCyc:   VVUL196600:VV1356-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 28401; Mature: 28401

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVARVAEVIDIPFCVAGGIKSAEDAARILEFGADKVSINSPALANPQLITDLADKF
ECHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHH
GVQCIVVGIDSYYDKETGKYQVYQFTGDEERTKATQWETRDWVQEVQKRGAGEIVLNMMN
CCEEEEEECCHHCCCCCCCEEEEEECCCHHHHHHHCCHHHHHHHHHHHCCCCCEEHHHHH
QDGVRNGYDIEQLNMVREVCNVPLIASGGAGAMEHFAEAYQKANVDGALAASVFHKQVIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
IGELKQYLKQQGIEVRL
HHHHHHHHHHCCCEECC
>Mature Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVARVAEVIDIPFCVAGGIKSAEDAARILEFGADKVSINSPALANPQLITDLADKF
ECHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHH
GVQCIVVGIDSYYDKETGKYQVYQFTGDEERTKATQWETRDWVQEVQKRGAGEIVLNMMN
CCEEEEEECCHHCCCCCCCEEEEEECCCHHHHHHHCCHHHHHHHHHHHCCCCCEEHHHHH
QDGVRNGYDIEQLNMVREVCNVPLIASGGAGAMEHFAEAYQKANVDGALAASVFHKQVIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
IGELKQYLKQQGIEVRL
HHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA