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The map label for this gene is nrfX [H]

Identifier: 37679439

GI number: 37679439

Start: 1274673

End: 1275194

Strand: Direct

Name: nrfX [H]

Synonym: VV1255

Alternate gene names: 37679439

Gene position: 1274673-1275194 (Clockwise)

Preceding gene: 37679438

Following gene: 37679440

Centisome position: 38.0

GC content: 41.19

Gene sequence:

>522_bases
GTGAACAACAAAGTTATTAAGTTGCTTGTGTTGGTGAGCATCGTCGGCATCGTTACGTTTACCGCAGTCTTGGGCATTGA
ACGACAAAATGCTGGTCACACAACGCCTGCCACTGTGATTCCTTTGCCCAATATTGACGCAAAAGATCTTTTTTCGGCGA
ATACGATCACAACGCAAGAGCTTATTTCCGATCAATACCGTGTGATGAACGTTTGGGCATCTTGGTGTGGGATTTGTCGA
GATGAGCATACGTTTATTATGTCGCTTAAACAGCAGGGTATTGACGTGATTGGCTTAAATTATCGTGATAATAAGCAAGA
AGCGTTGGGCTATTTGGAGAGTGAAGGTAACCCTTATTCCAAGGTGATTTATGATCCAGACGGGATCGTCTCAATCGATT
TTGGCGTGATTGGCACACCGGAAACCTACTTAATAAACCCTAATGGGGAAATAATGATCAAATTTCGAGGTAAATTAGAT
CAAAAACAGTGGAATAAACATTTTGCTACGTATTTTAAATAG

Upstream 100 bases:

>100_bases
GCTTACATTTGGTGGATCTGGCTAGGCGGTTTGATGGTTGTTGCTGCGGTGTTCACTGCATTACAACCAGTTCAGCAACG
CGTTAAGGTGGAGCGCTACT

Downstream 100 bases:

>100_bases
AACATTATTATCGACATGGCTCGTCTTATTCGTCGCTTTTGTCTCTTTCTCTAGTCAGGCACAGAACGAGAATACAAGCT
CTAATCACGTTGATTTGTTT

Product: thiol:disulfide interchange protein DsbE

Products: NA

Alternate protein names: Cytochrome c biogenesis protein NrfX [H]

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MNNKVIKLLVLVSIVGIVTFTAVLGIERQNAGHTTPATVIPLPNIDAKDLFSANTITTQELISDQYRVMNVWASWCGICR
DEHTFIMSLKQQGIDVIGLNYRDNKQEALGYLESEGNPYSKVIYDPDGIVSIDFGVIGTPETYLINPNGEIMIKFRGKLD
QKQWNKHFATYFK

Sequences:

>Translated_173_residues
MNNKVIKLLVLVSIVGIVTFTAVLGIERQNAGHTTPATVIPLPNIDAKDLFSANTITTQELISDQYRVMNVWASWCGICR
DEHTFIMSLKQQGIDVIGLNYRDNKQEALGYLESEGNPYSKVIYDPDGIVSIDFGVIGTPETYLINPNGEIMIKFRGKLD
QKQWNKHFATYFK
>Mature_173_residues
MNNKVIKLLVLVSIVGIVTFTAVLGIERQNAGHTTPATVIPLPNIDAKDLFSANTITTQELISDQYRVMNVWASWCGICR
DEHTFIMSLKQQGIDVIGLNYRDNKQEALGYLESEGNPYSKVIYDPDGIVSIDFGVIGTPETYLINPNGEIMIKFRGKLD
QKQWNKHFATYFK

Specific function: Could be involved in disulfide bond formation. Could catalyzes a late, reductive step in the assembly of periplasmic NrfA c-type cytochrome, probably the reduction of disulfide bonds of the apocytochrome c to allow covalent linkage with the heme. Possible

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein; Periplasmic side (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1788523, Length=177, Percent_Identity=31.638418079096, Blast_Score=113, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004799
- InterPro:   IPR013740
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF08534 Redoxin [H]

EC number: NA

Molecular weight: Translated: 19471; Mature: 19471

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKVIKLLVLVSIVGIVTFTAVLGIERQNAGHTTPATVIPLPNIDAKDLFSANTITTQE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHCCCCCCCHHH
LISDQYRVMNVWASWCGICRDEHTFIMSLKQQGIDVIGLNYRDNKQEALGYLESEGNPYS
HHHHHHHHHHHHHHHHHCCCCCCHHEEEHHHCCCEEEEECCCCCHHHHHHHHHCCCCCCE
KVIYDPDGIVSIDFGVIGTPETYLINPNGEIMIKFRGKLDQKQWNKHFATYFK
EEEECCCCEEEEECCEECCCCEEEECCCCEEEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNNKVIKLLVLVSIVGIVTFTAVLGIERQNAGHTTPATVIPLPNIDAKDLFSANTITTQE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHCCCCCCCHHH
LISDQYRVMNVWASWCGICRDEHTFIMSLKQQGIDVIGLNYRDNKQEALGYLESEGNPYS
HHHHHHHHHHHHHHHHHCCCCCCHHEEEHHHCCCEEEEECCCCCHHHHHHHHHCCCCCCE
KVIYDPDGIVSIDFGVIGTPETYLINPNGEIMIKFRGKLDQKQWNKHFATYFK
EEEECCCCEEEEECCEECCCCEEEECCCCEEEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]