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The map label for this gene is menE [H]

Identifier: 37679304

GI number: 37679304

Start: 1128311

End: 1129804

Strand: Direct

Name: menE [H]

Synonym: VV1120

Alternate gene names: 37679304

Gene position: 1128311-1129804 (Clockwise)

Preceding gene: 37679303

Following gene: 37679305

Centisome position: 33.64

GC content: 50.0

Gene sequence:

>1494_bases
ATGGCCGAAATGCTCGCTGCCGATCGCCTCGTTAGAAGAACAGCAATTGGTGTGGCATTCATAATGGGTGAAAAGGCATT
GGTCAGTGAACAACCATTGTGGAAATATTGGGCTCAGCAGAGCCCATTTTCTATGGCGCTGAACTCATCACGTTATCAAT
TCAACTGGCAACAATTGAACGACGAGATTGAACGTTTAGCGGCGGTTCTTGGTGCGCAAGGGGTGAGTCGTGGAGATGTC
ATCACTCTGCTGGGTAAAAACCATGTTGATATGGTGCTTATCTTGCTCGCTGCGCATCAACTGGGGGCTGTGGTGGCGAT
CATCATGCCGCAATCAATTGCAATGTTCTCAGCAAAGCTTGATACGCTTTACCGTGAGCAGCAAACGCGCTTTATTTGGC
TTGATCCTAACGTTGATGTGCAGACCCGCTCGGCTTGCTCTCACGATCGTCGTAATGTACTGCTAGAAATCTCACTCGCC
TCGGATCATCTTAACTCGATGCCTAGCAACCACGTTTGCGATTATCGCGCCGATCAACTTGCTACCATCATTTTTACCTC
TGGTTCCACGGGCAATCCCAAAGCGGTGGCTCATACTCATCGGCAGCATTTCGCGTCAGCACAAGGATTGTTAGAAGAGT
TCTGTTTTACTCAGCAAGATACATGGTTGCTCTCCCTGCCGCTGTATCATGTTTCTGGCATGGCTATTATTTATCGCTGG
TTGTTGGCTGGCGCATGTTTGAAAGTGGGGCATGGCACTTTGTCTGAGGACATTCAAGGGGTGACCCACGCGTCTCTCGT
CGCGACTCAATTGAAACGCCTGCTTGATGACAACACGCCTTTGCAACTGAGCCATGTTTTGCTGGGGGGGAGCCATGTGG
AGCATGCCTTGGCCCGAGCGGCGCAGCGTAAAGGAATAGAGACCTGGCTAGGCTACGGCATGACAGAGGCCGCTTCAACC
GTGACCGCCAAGCAGATCGACAGCGTGAGCAACGCAGGGCATCTATTGAAAAATCGAGCACTGCAACTGATTGGTCAGCG
CATTTATATCGGTGGTGAAACCTTGGCAGAAGGCTATTTCTTTCAGGGTAGCTTAACGACGCTGGTGGATGAGCATGGGT
GGTTTGACAGTAAAGATTTAGGCGTCTGGCAAGGTGAGGAGCTTAAGATCATCGGCCGTGCGGACAATCAGTTTATCTCT
GGCGGGGAAAACATCCACTGCGAAGAGATTGAAGCGGTGCTAAACGAGATTGAAGGTGTGGAACAGAGTATCGTGGTTCC
CATGCCCGATGCAGAATTTGGCTATCGACCAGTTGCTGTCATCCAATCGCCATCGCTAGCAAACAGAGCGCAGTATGAGC
AACACTTAAAAGAGAAACTGCAAAAGTTTAAGTGGCCAGTTGCCTATTACTTAATGCCTCTGACGCTACTGCAGGAAGGG
ATTAAAGTCTCGCGGCAAGCCGTTAAAGCATGGCTGGAAAATCAACTTAGTTGA

Upstream 100 bases:

>100_bases
TAGGGCTTACCCAGTTAGCGCGTTTTGCCAAATGGCAGTTGCCTGACGTGACGCCGGGCTTGGACACCATAAGTTTGTTC
CAATCGCAGTTAATGGTGGG

Downstream 100 bases:

>100_bases
TTTCGACATGAATTAGTCAAAAAGAGAATTTCTCAACCTTATAACCCTCAATCATAATTGCGCATCATATGACAAAGAAA
AGGCATAAGGTTGAAGATGG

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MAEMLAADRLVRRTAIGVAFIMGEKALVSEQPLWKYWAQQSPFSMALNSSRYQFNWQQLNDEIERLAAVLGAQGVSRGDV
ITLLGKNHVDMVLILLAAHQLGAVVAIIMPQSIAMFSAKLDTLYREQQTRFIWLDPNVDVQTRSACSHDRRNVLLEISLA
SDHLNSMPSNHVCDYRADQLATIIFTSGSTGNPKAVAHTHRQHFASAQGLLEEFCFTQQDTWLLSLPLYHVSGMAIIYRW
LLAGACLKVGHGTLSEDIQGVTHASLVATQLKRLLDDNTPLQLSHVLLGGSHVEHALARAAQRKGIETWLGYGMTEAAST
VTAKQIDSVSNAGHLLKNRALQLIGQRIYIGGETLAEGYFFQGSLTTLVDEHGWFDSKDLGVWQGEELKIIGRADNQFIS
GGENIHCEEIEAVLNEIEGVEQSIVVPMPDAEFGYRPVAVIQSPSLANRAQYEQHLKEKLQKFKWPVAYYLMPLTLLQEG
IKVSRQAVKAWLENQLS

Sequences:

>Translated_497_residues
MAEMLAADRLVRRTAIGVAFIMGEKALVSEQPLWKYWAQQSPFSMALNSSRYQFNWQQLNDEIERLAAVLGAQGVSRGDV
ITLLGKNHVDMVLILLAAHQLGAVVAIIMPQSIAMFSAKLDTLYREQQTRFIWLDPNVDVQTRSACSHDRRNVLLEISLA
SDHLNSMPSNHVCDYRADQLATIIFTSGSTGNPKAVAHTHRQHFASAQGLLEEFCFTQQDTWLLSLPLYHVSGMAIIYRW
LLAGACLKVGHGTLSEDIQGVTHASLVATQLKRLLDDNTPLQLSHVLLGGSHVEHALARAAQRKGIETWLGYGMTEAAST
VTAKQIDSVSNAGHLLKNRALQLIGQRIYIGGETLAEGYFFQGSLTTLVDEHGWFDSKDLGVWQGEELKIIGRADNQFIS
GGENIHCEEIEAVLNEIEGVEQSIVVPMPDAEFGYRPVAVIQSPSLANRAQYEQHLKEKLQKFKWPVAYYLMPLTLLQEG
IKVSRQAVKAWLENQLS
>Mature_496_residues
AEMLAADRLVRRTAIGVAFIMGEKALVSEQPLWKYWAQQSPFSMALNSSRYQFNWQQLNDEIERLAAVLGAQGVSRGDVI
TLLGKNHVDMVLILLAAHQLGAVVAIIMPQSIAMFSAKLDTLYREQQTRFIWLDPNVDVQTRSACSHDRRNVLLEISLAS
DHLNSMPSNHVCDYRADQLATIIFTSGSTGNPKAVAHTHRQHFASAQGLLEEFCFTQQDTWLLSLPLYHVSGMAIIYRWL
LAGACLKVGHGTLSEDIQGVTHASLVATQLKRLLDDNTPLQLSHVLLGGSHVEHALARAAQRKGIETWLGYGMTEAASTV
TAKQIDSVSNAGHLLKNRALQLIGQRIYIGGETLAEGYFFQGSLTTLVDEHGWFDSKDLGVWQGEELKIIGRADNQFISG
GENIHCEEIEAVLNEIEGVEQSIVVPMPDAEFGYRPVAVIQSPSLANRAQYEQHLKEKLQKFKWPVAYYLMPLTLLQEGI
KVSRQAVKAWLENQLS

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=470, Percent_Identity=37.2340425531915, Blast_Score=294, Evalue=7e-81,
Organism=Escherichia coli, GI145693145, Length=520, Percent_Identity=23.8461538461538, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788107, Length=327, Percent_Identity=24.4648318042813, Blast_Score=62, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI17558820, Length=436, Percent_Identity=24.7706422018349, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 55249; Mature: 55118

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEMLAADRLVRRTAIGVAFIMGEKALVSEQPLWKYWAQQSPFSMALNSSRYQFNWQQLN
CCHHHHHHHHHHHHHHHHHEEECCHHHCCCCHHHHHHCCCCCHHHHCCCCCEEEEHHHHH
DEIERLAAVLGAQGVSRGDVITLLGKNHVDMVLILLAAHQLGAVVAIIMPQSIAMFSAKL
HHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHH
DTLYREQQTRFIWLDPNVDVQTRSACSHDRRNVLLEISLASDHLNSMPSNHVCDYRADQL
HHHHHHCCCEEEEECCCCCCCCCHHHCCCCCCEEEEEEECHHHHHCCCCCCCCCCCCCCE
ATIIFTSGSTGNPKAVAHTHRQHFASAQGLLEEFCFTQQDTWLLSLPLYHVSGMAIIYRW
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHH
LLAGACLKVGHGTLSEDIQGVTHASLVATQLKRLLDDNTPLQLSHVLLGGSHVEHALARA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHCCHHHHHHHHHH
AQRKGIETWLGYGMTEAASTVTAKQIDSVSNAGHLLKNRALQLIGQRIYIGGETLAEGYF
HHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHCCEE
FQGSLTTLVDEHGWFDSKDLGVWQGEELKIIGRADNQFISGGENIHCEEIEAVLNEIEGV
ECCCHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCC
EQSIVVPMPDAEFGYRPVAVIQSPSLANRAQYEQHLKEKLQKFKWPVAYYLMPLTLLQEG
CCEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
IKVSRQAVKAWLENQLS
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AEMLAADRLVRRTAIGVAFIMGEKALVSEQPLWKYWAQQSPFSMALNSSRYQFNWQQLN
CHHHHHHHHHHHHHHHHHEEECCHHHCCCCHHHHHHCCCCCHHHHCCCCCEEEEHHHHH
DEIERLAAVLGAQGVSRGDVITLLGKNHVDMVLILLAAHQLGAVVAIIMPQSIAMFSAKL
HHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHH
DTLYREQQTRFIWLDPNVDVQTRSACSHDRRNVLLEISLASDHLNSMPSNHVCDYRADQL
HHHHHHCCCEEEEECCCCCCCCCHHHCCCCCCEEEEEEECHHHHHCCCCCCCCCCCCCCE
ATIIFTSGSTGNPKAVAHTHRQHFASAQGLLEEFCFTQQDTWLLSLPLYHVSGMAIIYRW
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHH
LLAGACLKVGHGTLSEDIQGVTHASLVATQLKRLLDDNTPLQLSHVLLGGSHVEHALARA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHCCHHHHHHHHHH
AQRKGIETWLGYGMTEAASTVTAKQIDSVSNAGHLLKNRALQLIGQRIYIGGETLAEGYF
HHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHCCEE
FQGSLTTLVDEHGWFDSKDLGVWQGEELKIIGRADNQFISGGENIHCEEIEAVLNEIEGV
ECCCHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCC
EQSIVVPMPDAEFGYRPVAVIQSPSLANRAQYEQHLKEKLQKFKWPVAYYLMPLTLLQEG
CCEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
IKVSRQAVKAWLENQLS
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8206837 [H]