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The map label for this gene is sucB [H]

Identifier: 37679217

GI number: 37679217

Start: 1033185

End: 1034393

Strand: Direct

Name: sucB [H]

Synonym: VV1033

Alternate gene names: 37679217

Gene position: 1033185-1034393 (Clockwise)

Preceding gene: 37679216

Following gene: 37679218

Centisome position: 30.8

GC content: 47.64

Gene sequence:

>1209_bases
ATGACTATTGAAATTCTGGTTCCAGATTTACCTGAATCAGTGGCCGATGCAACAGTTGCAACTTGGCACAAGAAACCAGG
CGACAGAGTTGAGCGCGATGAAGTACTGGTTGATATTGAAACAGACAAAGTAGTGTTAGAAGTACCGGCTTCAGAAGCGG
GTATTCTAGAAGCGATCGTTGAAGAAGAAGGTGCGACAGTGCTTTCGAAGCAACTGATTGGTCGCATTAAATTGGCGGCT
GTCGCGGGTGAACCTACGGCTGATACAACGGAAGAGTCAGAGCCATCTCCAGATAAGCGTCATACAGCAAGCTTATCAGA
AGAGAGCAATGATGCCCTTAGTCCAGCGGTGCGTCGCCTTCTCGCAGAACATAGCTTGGAAGCAAGCCAAGTAAAAGGTA
CGGGTGTTGGTGGCCGTATTACACGTGAAGATATCGAAGCGCACCTTGCCGCGGCAAAACAAGCTCCTGCAGTTGCAGAA
GCTCCTGCTGCAGTTGCACCGGTTGCTGCTCGCAGTGAAAAACGTGTTCCAATGACACGTCTACGCAAAACAGTGGCTAA
CCGTTTGCTAGAAGCGAAAAACAGCACGGCGATGTTGACGACGTTTAACGAAGTTAACATGAAGCCAATCATGGATCTTC
GTAAGCAGTATGGTGATCAGTTTGAAGCGCGTCATGGTATTCGTCTTGGCTTCATGTCTTTCTACGTGAAAGCGGTGACT
GAAGCACTGAAGCGTTATCCTGAAATCAACGCATCGATCGATGGTGATGACATTGTTTACCACAACTATTTTGACATCAG
TATGGCGGTTTCTACGCCACGTGGTCTAGTGACTCCAGTCCTAAAAGACTGCGACACGCTAGGTTTTGCGGATGTTGAAA
AGGGTATCAAAGAGCTCGCCATCAAAGGTCGTGACGGAAAACTAACCGTTGATGAGCTAATTGGCGGCAACTTTACCATC
ACGAATGGTGGTGTGTTTGGTTCATTGATGTCGACTCCAATCATCAACCCACCACAATCTGCGATTTTGGGTATGCATAA
GATTCAAGATCGTCCGATGGCGGTAGATGGCAAAGTTGAAATTCTGCCAATGATGTACCTAGCACTGTCTTATGACCACC
GCTTGATTGATGGTCGCGAGTCGGTTGGCTTCCTAGTGACCGTGAAAGAGTTACTGGAAGATCCAGCACGTCTGTTGTTA
GACGTTTAA

Upstream 100 bases:

>100_bases
TCGGTGCACTTGAAACAGCAGAAAGCGCTGATTGATGACGCGCTAAACGTGAACGAAAAGACTTCGGATTAAGAACTAGA
AGTAAAAGGAAGAAACAGAT

Downstream 100 bases:

>100_bases
TATCGCTAAAACGTCTAGTTGTCGTTGATGGCTAGACGTTAAAAATCCCTAAGGCTAGACTGGCTCAGCGTCTGGCCTTC
ACTTGAACTGTTAGCAGTTT

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTIEILVPDLPESVADATVATWHKKPGDRVERDEVLVDIETDKVVLEVPASEAGILEAIVEEEGATVLSKQLIGRIKLAA
VAGEPTADTTEESEPSPDKRHTASLSEESNDALSPAVRRLLAEHSLEASQVKGTGVGGRITREDIEAHLAAAKQAPAVAE
APAAVAPVAARSEKRVPMTRLRKTVANRLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGDQFEARHGIRLGFMSFYVKAVT
EALKRYPEINASIDGDDIVYHNYFDISMAVSTPRGLVTPVLKDCDTLGFADVEKGIKELAIKGRDGKLTVDELIGGNFTI
TNGGVFGSLMSTPIINPPQSAILGMHKIQDRPMAVDGKVEILPMMYLALSYDHRLIDGRESVGFLVTVKELLEDPARLLL
DV

Sequences:

>Translated_402_residues
MTIEILVPDLPESVADATVATWHKKPGDRVERDEVLVDIETDKVVLEVPASEAGILEAIVEEEGATVLSKQLIGRIKLAA
VAGEPTADTTEESEPSPDKRHTASLSEESNDALSPAVRRLLAEHSLEASQVKGTGVGGRITREDIEAHLAAAKQAPAVAE
APAAVAPVAARSEKRVPMTRLRKTVANRLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGDQFEARHGIRLGFMSFYVKAVT
EALKRYPEINASIDGDDIVYHNYFDISMAVSTPRGLVTPVLKDCDTLGFADVEKGIKELAIKGRDGKLTVDELIGGNFTI
TNGGVFGSLMSTPIINPPQSAILGMHKIQDRPMAVDGKVEILPMMYLALSYDHRLIDGRESVGFLVTVKELLEDPARLLL
DV
>Mature_401_residues
TIEILVPDLPESVADATVATWHKKPGDRVERDEVLVDIETDKVVLEVPASEAGILEAIVEEEGATVLSKQLIGRIKLAAV
AGEPTADTTEESEPSPDKRHTASLSEESNDALSPAVRRLLAEHSLEASQVKGTGVGGRITREDIEAHLAAAKQAPAVAEA
PAAVAPVAARSEKRVPMTRLRKTVANRLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGDQFEARHGIRLGFMSFYVKAVTE
ALKRYPEINASIDGDDIVYHNYFDISMAVSTPRGLVTPVLKDCDTLGFADVEKGIKELAIKGRDGKLTVDELIGGNFTIT
NGGVFGSLMSTPIINPPQSAILGMHKIQDRPMAVDGKVEILPMMYLALSYDHRLIDGRESVGFLVTVKELLEDPARLLLD
V

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=250, Percent_Identity=54.4, Blast_Score=279, Evalue=3e-75,
Organism=Homo sapiens, GI31711992, Length=431, Percent_Identity=29.9303944315545, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI203098753, Length=451, Percent_Identity=28.159645232816, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI203098816, Length=451, Percent_Identity=28.159645232816, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI110671329, Length=427, Percent_Identity=27.1662763466042, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=36.0248447204969, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1786946, Length=403, Percent_Identity=78.9081885856079, Blast_Score=639, Evalue=0.0,
Organism=Escherichia coli, GI1786305, Length=430, Percent_Identity=31.6279069767442, Blast_Score=165, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI25146366, Length=401, Percent_Identity=41.8952618453865, Blast_Score=310, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI17560088, Length=437, Percent_Identity=31.1212814645309, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=420, Percent_Identity=24.7619047619048, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=320, Percent_Identity=30.625, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320352, Length=400, Percent_Identity=42, Blast_Score=308, Evalue=1e-84,
Organism=Saccharomyces cerevisiae, GI6324258, Length=455, Percent_Identity=28.1318681318681, Blast_Score=150, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24645909, Length=229, Percent_Identity=57.6419213973799, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI18859875, Length=442, Percent_Identity=28.7330316742081, Blast_Score=155, Evalue=6e-38,
Organism=Drosophila melanogaster, GI20129315, Length=291, Percent_Identity=32.3024054982818, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24582497, Length=291, Percent_Identity=32.3024054982818, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43639; Mature: 43507

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEILVPDLPESVADATVATWHKKPGDRVERDEVLVDIETDKVVLEVPASEAGILEAIV
CEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCEEEEECCCCCHHHHHHHH
EEEGATVLSKQLIGRIKLAAVAGEPTADTTEESEPSPDKRHTASLSEESNDALSPAVRRL
HHCCHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHH
LAEHSLEASQVKGTGVGGRITREDIEAHLAAAKQAPAVAEAPAAVAPVAARSEKRVPMTR
HHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCHHCCHHHCCCCCCCHHH
LRKTVANRLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGDQFEARHGIRLGFMSFYVKAVT
HHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHH
EALKRYPEINASIDGDDIVYHNYFDISMAVSTPRGLVTPVLKDCDTLGFADVEKGIKELA
HHHHHCCCCCCCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHE
IKGRDGKLTVDELIGGNFTITNGGVFGSLMSTPIINPPQSAILGMHKIQDRPMAVDGKVE
ECCCCCCEEHHHHCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCCH
ILPMMYLALSYDHRLIDGRESVGFLVTVKELLEDPARLLLDV
HHHHHHHHHHCCCCEECCCCCCCHHEEHHHHHCCHHHHHCCC
>Mature Secondary Structure 
TIEILVPDLPESVADATVATWHKKPGDRVERDEVLVDIETDKVVLEVPASEAGILEAIV
EEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCEEEEECCCCCHHHHHHHH
EEEGATVLSKQLIGRIKLAAVAGEPTADTTEESEPSPDKRHTASLSEESNDALSPAVRRL
HHCCHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHH
LAEHSLEASQVKGTGVGGRITREDIEAHLAAAKQAPAVAEAPAAVAPVAARSEKRVPMTR
HHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCHHCCHHHCCCCCCCHHH
LRKTVANRLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGDQFEARHGIRLGFMSFYVKAVT
HHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHH
EALKRYPEINASIDGDDIVYHNYFDISMAVSTPRGLVTPVLKDCDTLGFADVEKGIKELA
HHHHHCCCCCCCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHE
IKGRDGKLTVDELIGGNFTITNGGVFGSLMSTPIINPPQSAILGMHKIQDRPMAVDGKVE
ECCCCCCEEHHHHCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCCH
ILPMMYLALSYDHRLIDGRESVGFLVTVKELLEDPARLLLDV
HHHHHHHHHHCCCCEECCCCCCCHHEEHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]