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The map label for this gene is hemK [H]

Identifier: 37679116

GI number: 37679116

Start: 932448

End: 933323

Strand: Direct

Name: hemK [H]

Synonym: VV0932

Alternate gene names: 37679116

Gene position: 932448-933323 (Clockwise)

Preceding gene: 37679115

Following gene: 37679117

Centisome position: 27.8

GC content: 49.2

Gene sequence:

>876_bases
ATGTCTGAGTTGATGACACTAGATGCCGCTGTTAAACAGGCGGCATCTCGCTTTTCTGAGTTAGGCAATGATTCGCCGTC
TTTAGACGCCGCGGTATTGCTTTGCCATGTACTGGATAAGCCGCGTAGCTATCTGTTTACCTGGCCAGATAAAATACTCA
CCGAACAAGAGCAGCAGCAGTTTGAAGCCTTAGTGGCGCGTCGACTGTCTGGCGAACCGGTGGCTTACATCATCGGTGAA
CGAGAGTTTTGGTCTTTGCCTTTTAAAGTCGCGCCATCGACGTTAATTCCTCGCCCCGATACCGAGCGTTTAGTGGAGTT
GGCGCTAGAAAAAACTGCGACGCAAACGGGCTCTATCCTCGACTTAGGCACAGGCACGGGAGCGATTGCCATTGCACTGG
CGTCTGAACTGCCACATCGCACTGTGATGGGCGTGGATTTGCAGCAGGAAGCGAAGCTGCTTGCTGAGTCGAATGCGTTG
GCATTGAATATCAAAAATGTGACCTTTAAACAGGGCAGCTGGTTTGAACCTGTGGTTCAAGGCACAAAGTTTGCTTTAAT
CGTTTCCAATCCTCCTTATATTGATGAGAATGATCCCCATCTTAATCAAGGAGATGTGCGATTTGAGCCGAAAAGTGCTC
TGGTTGCCGAAGAGTCTGGCCTGGCTGATATCCGTTATATCGCGCAACAGGCACGTGACTATCTTGAGCCTCATGGCTGG
CTGATGTTTGAACATGGCTACGACCAAGGTATCGCTGTTCGAGAAATCCTAGATACGTTGGGATATCAAGAGGTAGCAAC
CGAAAAGGATTACGGTGGCAATGATCGAGTGACTTTGGGTTACTTCGTTGCTTTACCAGAAAACACGGATCACTAG

Upstream 100 bases:

>100_bases
TCTGAAGTGATGGAAGGCGATCTTCAGTCTTTGATCGATCCTGTGATTCAGGAACACCAAGCGGACCAATTGGCTGCTTT
GGCGGAAAACAACTGATCCC

Downstream 100 bases:

>100_bases
ATCCAGATATCAAAGAGAACACTATGTACGAAGCTCTGAAATACTTTCATTTACTCACCATTGTCATCAGCGCGTTGCTG
CTTTCCGTTCGCTATGCATT

Product: methylase of polypeptide chain release factor

Products: NA

Alternate protein names: M.VchAHemK2P [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MSELMTLDAAVKQAASRFSELGNDSPSLDAAVLLCHVLDKPRSYLFTWPDKILTEQEQQQFEALVARRLSGEPVAYIIGE
REFWSLPFKVAPSTLIPRPDTERLVELALEKTATQTGSILDLGTGTGAIAIALASELPHRTVMGVDLQQEAKLLAESNAL
ALNIKNVTFKQGSWFEPVVQGTKFALIVSNPPYIDENDPHLNQGDVRFEPKSALVAEESGLADIRYIAQQARDYLEPHGW
LMFEHGYDQGIAVREILDTLGYQEVATEKDYGGNDRVTLGYFVALPENTDH

Sequences:

>Translated_291_residues
MSELMTLDAAVKQAASRFSELGNDSPSLDAAVLLCHVLDKPRSYLFTWPDKILTEQEQQQFEALVARRLSGEPVAYIIGE
REFWSLPFKVAPSTLIPRPDTERLVELALEKTATQTGSILDLGTGTGAIAIALASELPHRTVMGVDLQQEAKLLAESNAL
ALNIKNVTFKQGSWFEPVVQGTKFALIVSNPPYIDENDPHLNQGDVRFEPKSALVAEESGLADIRYIAQQARDYLEPHGW
LMFEHGYDQGIAVREILDTLGYQEVATEKDYGGNDRVTLGYFVALPENTDH
>Mature_290_residues
SELMTLDAAVKQAASRFSELGNDSPSLDAAVLLCHVLDKPRSYLFTWPDKILTEQEQQQFEALVARRLSGEPVAYIIGER
EFWSLPFKVAPSTLIPRPDTERLVELALEKTATQTGSILDLGTGTGAIAIALASELPHRTVMGVDLQQEAKLLAESNALA
LNIKNVTFKQGSWFEPVVQGTKFALIVSNPPYIDENDPHLNQGDVRFEPKSALVAEESGLADIRYIAQQARDYLEPHGWL
MFEHGYDQGIAVREILDTLGYQEVATEKDYGGNDRVTLGYFVALPENTDH

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=266, Percent_Identity=33.0827067669173, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1787463, Length=261, Percent_Identity=53.2567049808429, Blast_Score=276, Evalue=1e-75,
Organism=Escherichia coli, GI87082085, Length=255, Percent_Identity=31.3725490196078, Blast_Score=91, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24582226, Length=258, Percent_Identity=27.5193798449612, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 32230; Mature: 32098

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELMTLDAAVKQAASRFSELGNDSPSLDAAVLLCHVLDKPRSYLFTWPDKILTEQEQQQ
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCHHHHCHHHHHH
FEALVARRLSGEPVAYIIGEREFWSLPFKVAPSTLIPRPDTERLVELALEKTATQTGSIL
HHHHHHHHCCCCCEEEEECCCHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEE
DLGTGTGAIAIALASELPHRTVMGVDLQQEAKLLAESNALALNIKNVTFKQGSWFEPVVQ
EECCCCCCEEEEEHHHCCCCEEECCCHHHHHHHHHCCCEEEEEEECEEECCCCCCHHHHC
GTKFALIVSNPPYIDENDPHLNQGDVRFEPKSALVAEESGLADIRYIAQQARDYLEPHGW
CCEEEEEECCCCCCCCCCCCCCCCCEEECCCHHEEECCCCCHHHHHHHHHHHHHHCCCCE
LMFEHGYDQGIAVREILDTLGYQEVATEKDYGGNDRVTLGYFVALPENTDH
EEEECCCCCCHHHHHHHHHCCHHHHHCCCCCCCCCEEEEEEEEEECCCCCC
>Mature Secondary Structure 
SELMTLDAAVKQAASRFSELGNDSPSLDAAVLLCHVLDKPRSYLFTWPDKILTEQEQQQ
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCHHHHCHHHHHH
FEALVARRLSGEPVAYIIGEREFWSLPFKVAPSTLIPRPDTERLVELALEKTATQTGSIL
HHHHHHHHCCCCCEEEEECCCHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEE
DLGTGTGAIAIALASELPHRTVMGVDLQQEAKLLAESNALALNIKNVTFKQGSWFEPVVQ
EECCCCCCEEEEEHHHCCCCEEECCCHHHHHHHHHCCCEEEEEEECEEECCCCCCHHHHC
GTKFALIVSNPPYIDENDPHLNQGDVRFEPKSALVAEESGLADIRYIAQQARDYLEPHGW
CCEEEEEECCCCCCCCCCCCCCCCCEEECCCHHEEECCCCCHHHHHHHHHHHHHHCCCCE
LMFEHGYDQGIAVREILDTLGYQEVATEKDYGGNDRVTLGYFVALPENTDH
EEEECCCCCCHHHHHHHHHCCHHHHHCCCCCCCCCEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]