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The map label for this gene is ubiF [H]

Identifier: 37679102

GI number: 37679102

Start: 922139

End: 923359

Strand: Direct

Name: ubiF [H]

Synonym: VV0918

Alternate gene names: 37679102

Gene position: 922139-923359 (Clockwise)

Preceding gene: 37679096

Following gene: 37679105

Centisome position: 27.49

GC content: 47.01

Gene sequence:

>1221_bases
GTGTTTCGCAAACTAGCCAGTAAAACGTACAATGCAAAACCGTCTTTGAATCAAGTTGATCAAGAGTACAAGATGAATAA
ATACGACATTGCCGTCGTCGGTGGCGGCATGGTAGGCGCGGCTGTGGCCATCGGATTTGCCAAACAAGGCAGAAAAGTCA
TCGTGGTTGAAGGGTTTGAGCCGAAAGCGTTTGAAGCAAGCCAACCGATGGACATCCGAGTGTCCGCCATTTCCCATAAC
TCCGTGCGTTTGCTGGAATCGCTAGGGGCCTGGCAACATATTCAAGCAACTCGAGTTTGTCCCTACAAACGCTTAGAAAC
CTGGGAGCACCCAGAATGTCGTACACGTTTTCACAGCGATGAACTCAATTTGGAACAGCTAGGTTACATTGTGGAGAACC
GTCTAATTCAATTGGGGTTGTGGCAAGAGTTTGCTCAATACCCGAATTTGACTCTGCAATGCCCAGATAAACTGAAGCGT
CTTGAGTTCGATGAGATCAACACGTTGACGCTTGAGAGTGGTTTAGAAATTGAAGCCTCTTGGGTCATTGGCGCAGATGG
TGCCAACTCTCAGGTGCGTGAGTTGGCTGGCATTGGCATTACCGCATGGGATTATCGTCAACATTGTATGTTGATCAATA
TTGCGACGGAATCTGCGCAACAGGCGATCACTTGGCAGCAATTCACTCCAACTGGCCCACGATCTTTTTTGCCATTGAAA
GGCCAGCAAGGTTCTTTAGTTTGGTACGATTCACCCAAACGTATTCGTGAGTTGTCAGCCATGAGCGCTGAAAAGCTGAA
ACAGGAAATTATTCGTCATTTCCCTGATGAATTGGGAGAGTTTGACGTGCTGTACTGGGATTCTTTCCCATTAACCCGAC
GACATGCACAAGCGTATTATCAACAAAATTGTTTGTTGGTTGGCGATGCGGCCCACACGATCAATCCATTAGCTGGCCAA
GGTGTCAATCTTGGTTTTAAAGACGTTGAAGCGTTACTCAAGGCGACGGAAAACCAACCTTTGACAGTGGCTCAACAAGC
GGCCTATCAGAAAGCACGCTATGCGGACAACTTGCTAATGCAAACTGGGATGGATCTGTTCTACAAAGGCTTTAGTAACG
ATATCACCCCATTAAAATTGGTTCGTAACGCAGCCCTCAAGTTGGCTGAGCACTCTGGGCCTATCAAACATCAAGTGTTG
AAATATGCGCTAGGAATGTGA

Upstream 100 bases:

>100_bases
AGCCTGATTTTATCTAGGGTCTTGTCAGGTATTTCGCCTTGGTTTTTTAAGGTTTTTGTCGATGGAAACTGAGTGACCTA
AGCCGCTTGATAGGGACAAA

Downstream 100 bases:

>100_bases
TGGCATTGGGAGAGGGCTCATAAACGAGCGTTTGTATCAGGAAAGGAAAGCGGACTCATCGAGTTCGCTTTTTTTTGTTT
TCACAAACAATGAACAATGC

Product: 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MFRKLASKTYNAKPSLNQVDQEYKMNKYDIAVVGGGMVGAAVAIGFAKQGRKVIVVEGFEPKAFEASQPMDIRVSAISHN
SVRLLESLGAWQHIQATRVCPYKRLETWEHPECRTRFHSDELNLEQLGYIVENRLIQLGLWQEFAQYPNLTLQCPDKLKR
LEFDEINTLTLESGLEIEASWVIGADGANSQVRELAGIGITAWDYRQHCMLINIATESAQQAITWQQFTPTGPRSFLPLK
GQQGSLVWYDSPKRIRELSAMSAEKLKQEIIRHFPDELGEFDVLYWDSFPLTRRHAQAYYQQNCLLVGDAAHTINPLAGQ
GVNLGFKDVEALLKATENQPLTVAQQAAYQKARYADNLLMQTGMDLFYKGFSNDITPLKLVRNAALKLAEHSGPIKHQVL
KYALGM

Sequences:

>Translated_406_residues
MFRKLASKTYNAKPSLNQVDQEYKMNKYDIAVVGGGMVGAAVAIGFAKQGRKVIVVEGFEPKAFEASQPMDIRVSAISHN
SVRLLESLGAWQHIQATRVCPYKRLETWEHPECRTRFHSDELNLEQLGYIVENRLIQLGLWQEFAQYPNLTLQCPDKLKR
LEFDEINTLTLESGLEIEASWVIGADGANSQVRELAGIGITAWDYRQHCMLINIATESAQQAITWQQFTPTGPRSFLPLK
GQQGSLVWYDSPKRIRELSAMSAEKLKQEIIRHFPDELGEFDVLYWDSFPLTRRHAQAYYQQNCLLVGDAAHTINPLAGQ
GVNLGFKDVEALLKATENQPLTVAQQAAYQKARYADNLLMQTGMDLFYKGFSNDITPLKLVRNAALKLAEHSGPIKHQVL
KYALGM
>Mature_406_residues
MFRKLASKTYNAKPSLNQVDQEYKMNKYDIAVVGGGMVGAAVAIGFAKQGRKVIVVEGFEPKAFEASQPMDIRVSAISHN
SVRLLESLGAWQHIQATRVCPYKRLETWEHPECRTRFHSDELNLEQLGYIVENRLIQLGLWQEFAQYPNLTLQCPDKLKR
LEFDEINTLTLESGLEIEASWVIGADGANSQVRELAGIGITAWDYRQHCMLINIATESAQQAITWQQFTPTGPRSFLPLK
GQQGSLVWYDSPKRIRELSAMSAEKLKQEIIRHFPDELGEFDVLYWDSFPLTRRHAQAYYQQNCLLVGDAAHTINPLAGQ
GVNLGFKDVEALLKATENQPLTVAQQAAYQKARYADNLLMQTGMDLFYKGFSNDITPLKLVRNAALKLAEHSGPIKHQVL
KYALGM

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=420, Percent_Identity=28.8095238095238, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI32967307, Length=337, Percent_Identity=27.893175074184, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1786883, Length=385, Percent_Identity=52.2077922077922, Blast_Score=390, Evalue=1e-110,
Organism=Escherichia coli, GI1789273, Length=392, Percent_Identity=38.0102040816327, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI1789274, Length=394, Percent_Identity=28.4263959390863, Blast_Score=138, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI25146631, Length=431, Percent_Identity=29.0023201856148, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6321694, Length=449, Percent_Identity=24.2761692650334, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19920760, Length=461, Percent_Identity=29.5010845986985, Blast_Score=180, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 45817; Mature: 45817

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRKLASKTYNAKPSLNQVDQEYKMNKYDIAVVGGGMVGAAVAIGFAKQGRKVIVVEGFE
CCHHHHCCCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCC
PKAFEASQPMDIRVSAISHNSVRLLESLGAWQHIQATRVCPYKRLETWEHPECRTRFHSD
CCCCCCCCCCEEEEEEECCCHHHHHHHCCCHHHHHHHHCCCHHHHCCCCCCHHHHCCCCC
ELNLEQLGYIVENRLIQLGLWQEFAQYPNLTLQCPDKLKRLEFDEINTLTLESGLEIEAS
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCCCCCEEEECCCCEEEEE
WVIGADGANSQVRELAGIGITAWDYRQHCMLINIATESAQQAITWQQFTPTGPRSFLPLK
EEEECCCCCHHHHHHHCCCCEEEECCCCEEEEEEECHHHHHHEEEEECCCCCCCCCCCCC
GQQGSLVWYDSPKRIRELSAMSAEKLKQEIIRHFPDELGEFDVLYWDSFPLTRRHAQAYY
CCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCEEEEEECCCCCHHHHHHHHH
QQNCLLVGDAAHTINPLAGQGVNLGFKDVEALLKATENQPLTVAQQAAYQKARYADNLLM
HCCCEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
QTGMDLFYKGFSNDITPLKLVRNAALKLAEHSGPIKHQVLKYALGM
HHCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MFRKLASKTYNAKPSLNQVDQEYKMNKYDIAVVGGGMVGAAVAIGFAKQGRKVIVVEGFE
CCHHHHCCCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCC
PKAFEASQPMDIRVSAISHNSVRLLESLGAWQHIQATRVCPYKRLETWEHPECRTRFHSD
CCCCCCCCCCEEEEEEECCCHHHHHHHCCCHHHHHHHHCCCHHHHCCCCCCHHHHCCCCC
ELNLEQLGYIVENRLIQLGLWQEFAQYPNLTLQCPDKLKRLEFDEINTLTLESGLEIEAS
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCCCCCEEEECCCCEEEEE
WVIGADGANSQVRELAGIGITAWDYRQHCMLINIATESAQQAITWQQFTPTGPRSFLPLK
EEEECCCCCHHHHHHHCCCCEEEECCCCEEEEEEECHHHHHHEEEEECCCCCCCCCCCCC
GQQGSLVWYDSPKRIRELSAMSAEKLKQEIIRHFPDELGEFDVLYWDSFPLTRRHAQAYY
CCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCEEEEEECCCCCHHHHHHHHH
QQNCLLVGDAAHTINPLAGQGVNLGFKDVEALLKATENQPLTVAQQAAYQKARYADNLLM
HCCCEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
QTGMDLFYKGFSNDITPLKLVRNAALKLAEHSGPIKHQVLKYALGM
HHCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]