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The map label for this gene is aceA [H]

Identifier: 37678926

GI number: 37678926

Start: 748085

End: 749458

Strand: Direct

Name: aceA [H]

Synonym: VV0742

Alternate gene names: 37678926

Gene position: 748085-749458 (Clockwise)

Preceding gene: 37678925

Following gene: 37678928

Centisome position: 22.3

GC content: 49.13

Gene sequence:

>1374_bases
ATGGAATATATAGCGCACTCAATTTTAATAATCAGCGCTCATCAAAAGAAGGATAGACCGATGACAAACTTAACTCGCCG
CCAGCAAATCGAAGCTCTGGAAAAAGATTGGGCAACCAATCCTCGTTGGAAAAACGTCAAACGCACTTACACAGCTGAGG
AAGTGGTGGCCTTACGTGGCTCTATGGTGCCAGCTAATACCATCGCGCAGCGTGGTGCCGACAAGTTGTGGTCGTTGGTG
AATGGTAGCTCGAAAAAAGGCTATGTGAATTGTCTAGGCGCACTAACTGGTGGCCAAGCGGTGCAGCAAGCCAAAGCGGG
GATAGAAGCGATCTACCTATCGGGTTGGCAGGTTGCAGCGGATAACAACACCGCCTCGACCATGTACCCAGACCAATCAC
TTTACCCTGTGGATTCGGTTCCTTCAGTAGTCAAACGCATTAACAATTCGTTCCGCCGCGCAGACCAAATTCAATGGTCG
AATGGTAAGTCGCCAGAAGAGGGTGGAATTGATTACTTCTTGCCTATCGTTGCTGATGCAGAAGCGGGCTTTGGTGGTGT
GCTGAATGCGTATGAGCTGATGAAGTCGATGATTGAAGCAGGGGCCGCGGGTGTACACTTTGAAGACCAATTGGCTTCGG
TGAAGAAGTGTGGCCATATGGGCGGTAAAGTATTGGTTCCAACGCAAGAAGCGGTGCAAAAACTGGTTGCTGCGCGTCTA
GCCGCAGATGTTGCTGGTACAACGACATTGGTGATTGCTCGAACTGATGCCAATGCGGCGGATTTGTTGACGTCAGATTG
TGACCCATATGACGCAGACTTTATTGTTGGCGAGCGCACTCAGGAAGGTTTCTATCGTGTTCGTGCAGGGATTGACCAAG
CGATCTCACGTGGCCTTGCTTATGCGCCATATGCGGATCTTATTTGGTGTGAAACGGCAACTCCGTGTCTTGAAGAAGCG
CGTAAGTTTGCTGAGGCTATTCATGCGGAATACCCAGACCAACTGTTGGCATACAACTGCTCGCCATCATTTAACTGGGA
GAAAAACCTGGATGCTGAGACCATTGCTAAGTTCCAACAAGAACTCGCAGATATGGGTTACAAGTACCAGTTCATCACCC
TAGCTGGTATTCACAACATGTGGTTCAACATGTTTGAACTGGCACACGCATATGCTCAAGGTGAAGGTATGCGTCACTAC
GTTGAGAAAGTTCAGCGTCCTGAATTTGAAGCGGCGGAAAAAGGCTACACCTTCGTTGCACACCAACAAGAAGTGGGTAC
GGGTTACTTTGATAAGATGACCAACACCATCCAAGGTGGTAACTCTTCAGTCACGGCACTGACGGGCTCTACCGAAGAAG
ACCAGTTCCATTAA

Upstream 100 bases:

>100_bases
TTAACCGTACCGGGTTATGACTACCTAGATTAATGAGTAAACCAATCAACAACCAATAACGTGAACCTTCATTGGCGGAG
CAGGATGTACCGCTAACGAA

Downstream 100 bases:

>100_bases
TACCTTTTCATCTTTAGGTATTAACCACTGCAAGAGCGGCGAAAGCCGCTCTTTTTCCTTCCCTATCAGGACAAACAAGG
ATCTCGCAGCTAAGAGTGTC

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MEYIAHSILIISAHQKKDRPMTNLTRRQQIEALEKDWATNPRWKNVKRTYTAEEVVALRGSMVPANTIAQRGADKLWSLV
NGSSKKGYVNCLGALTGGQAVQQAKAGIEAIYLSGWQVAADNNTASTMYPDQSLYPVDSVPSVVKRINNSFRRADQIQWS
NGKSPEEGGIDYFLPIVADAEAGFGGVLNAYELMKSMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARL
AADVAGTTTLVIARTDANAADLLTSDCDPYDADFIVGERTQEGFYRVRAGIDQAISRGLAYAPYADLIWCETATPCLEEA
RKFAEAIHAEYPDQLLAYNCSPSFNWEKNLDAETIAKFQQELADMGYKYQFITLAGIHNMWFNMFELAHAYAQGEGMRHY
VEKVQRPEFEAAEKGYTFVAHQQEVGTGYFDKMTNTIQGGNSSVTALTGSTEEDQFH

Sequences:

>Translated_457_residues
MEYIAHSILIISAHQKKDRPMTNLTRRQQIEALEKDWATNPRWKNVKRTYTAEEVVALRGSMVPANTIAQRGADKLWSLV
NGSSKKGYVNCLGALTGGQAVQQAKAGIEAIYLSGWQVAADNNTASTMYPDQSLYPVDSVPSVVKRINNSFRRADQIQWS
NGKSPEEGGIDYFLPIVADAEAGFGGVLNAYELMKSMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARL
AADVAGTTTLVIARTDANAADLLTSDCDPYDADFIVGERTQEGFYRVRAGIDQAISRGLAYAPYADLIWCETATPCLEEA
RKFAEAIHAEYPDQLLAYNCSPSFNWEKNLDAETIAKFQQELADMGYKYQFITLAGIHNMWFNMFELAHAYAQGEGMRHY
VEKVQRPEFEAAEKGYTFVAHQQEVGTGYFDKMTNTIQGGNSSVTALTGSTEEDQFH
>Mature_457_residues
MEYIAHSILIISAHQKKDRPMTNLTRRQQIEALEKDWATNPRWKNVKRTYTAEEVVALRGSMVPANTIAQRGADKLWSLV
NGSSKKGYVNCLGALTGGQAVQQAKAGIEAIYLSGWQVAADNNTASTMYPDQSLYPVDSVPSVVKRINNSFRRADQIQWS
NGKSPEEGGIDYFLPIVADAEAGFGGVLNAYELMKSMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARL
AADVAGTTTLVIARTDANAADLLTSDCDPYDADFIVGERTQEGFYRVRAGIDQAISRGLAYAPYADLIWCETATPCLEEA
RKFAEAIHAEYPDQLLAYNCSPSFNWEKNLDAETIAKFQQELADMGYKYQFITLAGIHNMWFNMFELAHAYAQGEGMRHY
VEKVQRPEFEAAEKGYTFVAHQQEVGTGYFDKMTNTIQGGNSSVTALTGSTEEDQFH

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=433, Percent_Identity=77.5981524249423, Blast_Score=718, Evalue=0.0,
Organism=Escherichia coli, GI1786525, Length=268, Percent_Identity=29.1044776119403, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71982926, Length=419, Percent_Identity=57.0405727923628, Blast_Score=472, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI17561814, Length=419, Percent_Identity=57.0405727923628, Blast_Score=471, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6320908, Length=290, Percent_Identity=38.2758620689655, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6325263, Length=249, Percent_Identity=37.7510040160643, Blast_Score=162, Evalue=9e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 50366; Mature: 50366

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYIAHSILIISAHQKKDRPMTNLTRRQQIEALEKDWATNPRWKNVKRTYTAEEVVALRG
CCCHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHC
SMVPANTIAQRGADKLWSLVNGSSKKGYVNCLGALTGGQAVQQAKAGIEAIYLSGWQVAA
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHCCCEEEEECCEEEEE
DNNTASTMYPDQSLYPVDSVPSVVKRINNSFRRADQIQWSNGKSPEEGGIDYFLPIVADA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECC
EAGFGGVLNAYELMKSMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARL
CCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHH
AADVAGTTTLVIARTDANAADLLTSDCDPYDADFIVGERTQEGFYRVRAGIDQAISRGLA
HHHHCCCEEEEEEECCCCHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCC
YAPYADLIWCETATPCLEEARKFAEAIHAEYPDQLLAYNCSPSFNWEKNLDAETIAKFQQ
CCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCHHEEEECCCCCCCCCCCCHHHHHHHHH
ELADMGYKYQFITLAGIHNMWFNMFELAHAYAQGEGMRHYVEKVQRPEFEAAEKGYTFVA
HHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHCCCEEEE
HQQEVGTGYFDKMTNTIQGGNSSVTALTGSTEEDQFH
EHHHHCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MEYIAHSILIISAHQKKDRPMTNLTRRQQIEALEKDWATNPRWKNVKRTYTAEEVVALRG
CCCHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHC
SMVPANTIAQRGADKLWSLVNGSSKKGYVNCLGALTGGQAVQQAKAGIEAIYLSGWQVAA
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHCCCEEEEECCEEEEE
DNNTASTMYPDQSLYPVDSVPSVVKRINNSFRRADQIQWSNGKSPEEGGIDYFLPIVADA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECC
EAGFGGVLNAYELMKSMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARL
CCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHH
AADVAGTTTLVIARTDANAADLLTSDCDPYDADFIVGERTQEGFYRVRAGIDQAISRGLA
HHHHCCCEEEEEEECCCCHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCC
YAPYADLIWCETATPCLEEARKFAEAIHAEYPDQLLAYNCSPSFNWEKNLDAETIAKFQQ
CCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCHHEEEECCCCCCCCCCCCHHHHHHHHH
ELADMGYKYQFITLAGIHNMWFNMFELAHAYAQGEGMRHYVEKVQRPEFEAAEKGYTFVA
HHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHCCCEEEE
HQQEVGTGYFDKMTNTIQGGNSSVTALTGSTEEDQFH
EHHHHCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]