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The map label for this gene is mepA

Identifier: 37678903

GI number: 37678903

Start: 723024

End: 723854

Strand: Direct

Name: mepA

Synonym: VV0719

Alternate gene names: 37678903

Gene position: 723024-723854 (Clockwise)

Preceding gene: 37678899

Following gene: 37678907

Centisome position: 21.55

GC content: 47.41

Gene sequence:

>831_bases
ATGTTCGCTAAAATACTGGCATCATTGTGCACGATGTATTCAATACCTATTTGGGCATTGCAAATGACAGCGTGGGAATT
GGTGACTGCACCAACGCGTGAACGTGCTCAATCAATAGGATCGTACGCAAATGGCTGTTTAGATGGTGGTGTCGCTTTAC
CACTTGAGGGGGCGGGGTATCAAGTTGTGCGTACAGAGCGTAATCGCTACTTTGCGCATCCAGAAACTGTCAATTTTATA
CAAGAGTTAGGCTATTACGCGGATACACATTTGGCTTCGACTCTAATGATTGCGGATGTCAGTTTACCACGAGGTGGGCG
TTTTTCTTTTGGTCATGCGAGCCATCAAACCGGACTCGATATTGATGTATGGCTTAAACTAAACACCTCTCCTTTGAATA
AATATGAGCTTGAACAGGCAAAGACAGAGAGTGTCGTCAATTTCGATACCATGACTATTCGTGCCGATGAATGGCGTGAT
GAGCAATTCGAGTTGATCCGAGCCGCAGCCGAAAATGGCAGGGTAGCGAGAATTTTTGTCCACGCCGTTATCAAGCAACG
TTTATGTCAAATGGAAACATCCACTCATAGGTCGTGGCTGAGAAAGGTAAGGCCTTGGTGGGGACATTCTTCACACATGC
ACGTGAGACTCTCTTGCCCACAAGGAAGCGATGACTGTATCGAGCAAGCTTTGCCGCCGGAGGGAGATGGTTGTGGTGAA
GAAGTGTTGAGCTGGATAGAGCGCCCAACACCAACGGCGAAAAAAGCACAGGAGCCCGAGCCTATCTCTGAGGCATGTGT
GACGGTGCTAAATCAGAAACGTGAGAAATAA

Upstream 100 bases:

>100_bases
TACTTGTATTTCACGCATTTGTTCACTTCAATTGAAATCATGACTTCCTAGTCGCAATATGCTTTAGAAACCTATCTTCA
CAATAAGTAAGGATCAATGG

Downstream 100 bases:

>100_bases
ATAAAGCGCCCATTGGCGCTTTATTTGATATCGATTAGGCTAAGCCTTGGATGATGACAAACTTTTCAAGCAGTTGCTCT
TCCGTTTCGATGTGGTTTGG

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: D-alanyl-D-alanine-endopeptidase; DD-endopeptidase [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MFAKILASLCTMYSIPIWALQMTAWELVTAPTRERAQSIGSYANGCLDGGVALPLEGAGYQVVRTERNRYFAHPETVNFI
QELGYYADTHLASTLMIADVSLPRGGRFSFGHASHQTGLDIDVWLKLNTSPLNKYELEQAKTESVVNFDTMTIRADEWRD
EQFELIRAAAENGRVARIFVHAVIKQRLCQMETSTHRSWLRKVRPWWGHSSHMHVRLSCPQGSDDCIEQALPPEGDGCGE
EVLSWIERPTPTAKKAQEPEPISEACVTVLNQKREK

Sequences:

>Translated_276_residues
MFAKILASLCTMYSIPIWALQMTAWELVTAPTRERAQSIGSYANGCLDGGVALPLEGAGYQVVRTERNRYFAHPETVNFI
QELGYYADTHLASTLMIADVSLPRGGRFSFGHASHQTGLDIDVWLKLNTSPLNKYELEQAKTESVVNFDTMTIRADEWRD
EQFELIRAAAENGRVARIFVHAVIKQRLCQMETSTHRSWLRKVRPWWGHSSHMHVRLSCPQGSDDCIEQALPPEGDGCGE
EVLSWIERPTPTAKKAQEPEPISEACVTVLNQKREK
>Mature_276_residues
MFAKILASLCTMYSIPIWALQMTAWELVTAPTRERAQSIGSYANGCLDGGVALPLEGAGYQVVRTERNRYFAHPETVNFI
QELGYYADTHLASTLMIADVSLPRGGRFSFGHASHQTGLDIDVWLKLNTSPLNKYELEQAKTESVVNFDTMTIRADEWRD
EQFELIRAAAENGRVARIFVHAVIKQRLCQMETSTHRSWLRKVRPWWGHSSHMHVRLSCPQGSDDCIEQALPPEGDGCGE
EVLSWIERPTPTAKKAQEPEPISEACVTVLNQKREK

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=252, Percent_Identity=44.8412698412698, Blast_Score=239, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 31132; Mature: 31132

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00477 ALPHA_2_MACROGLOBULIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAKILASLCTMYSIPIWALQMTAWELVTAPTRERAQSIGSYANGCLDGGVALPLEGAGY
CHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCE
QVVRTERNRYFAHPETVNFIQELGYYADTHLASTLMIADVSLPRGGRFSFGHASHQTGLD
EEEEECCCCEECCCHHHHHHHHHCCHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCC
IDVWLKLNTSPLNKYELEQAKTESVVNFDTMTIRADEWRDEQFELIRAAAENGRVARIFV
EEEEEEECCCCCCHHHHHHHHHHHHEEEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHH
HAVIKQRLCQMETSTHRSWLRKVRPWWGHSSHMHVRLSCPQGSDDCIEQALPPEGDGCGE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCHH
EVLSWIERPTPTAKKAQEPEPISEACVTVLNQKREK
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFAKILASLCTMYSIPIWALQMTAWELVTAPTRERAQSIGSYANGCLDGGVALPLEGAGY
CHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCE
QVVRTERNRYFAHPETVNFIQELGYYADTHLASTLMIADVSLPRGGRFSFGHASHQTGLD
EEEEECCCCEECCCHHHHHHHHHCCHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCC
IDVWLKLNTSPLNKYELEQAKTESVVNFDTMTIRADEWRDEQFELIRAAAENGRVARIFV
EEEEEEECCCCCCHHHHHHHHHHHHEEEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHH
HAVIKQRLCQMETSTHRSWLRKVRPWWGHSSHMHVRLSCPQGSDDCIEQALPPEGDGCGE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCHH
EVLSWIERPTPTAKKAQEPEPISEACVTVLNQKREK
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA