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The map label for this gene is clpB [H]

Identifier: 37678899

GI number: 37678899

Start: 714179

End: 716752

Strand: Direct

Name: clpB [H]

Synonym: VV0715

Alternate gene names: 37678899

Gene position: 714179-716752 (Clockwise)

Preceding gene: 37678898

Following gene: 37678903

Centisome position: 21.29

GC content: 47.28

Gene sequence:

>2574_bases
ATGCGTCTTGATCGATTTACCAGCAAATTTCAAATCGCTATCTCTGATGCACAATCATTGGCGTTAGGGCGCGATCATCA
ATACATTGAACCTGTTCACTTAATGGTGGCGCTGCTTGATCAAAACGGCAGCCCTATTCGTCCACTGCTGACCATCTTAA
ATGTGGATGTCACTCACCTACGTTCAAAACTCAGCGAAATGTTGGATCGCTTACCCAAAGTGAGCGGTATTGGTGGTGAT
GTACAGCTTTCCTCAGCCATGGGCGCGATGTTTAACTTGTGCGATAAGATTGCACAAAAACGCCAAGATGCTTACATCTC
ATCTGAAATCTTTTTGCTCGCAGCCATCGAAGATAGAGGACCACTTGGCCAGCTCTTCAAAGAACTTGGCTTAACTGAAC
AGAAAGTGAGTCAAGCTATCGAGCAAATTCGCGGTGGTCAAAAAGTGAATGATCAAAATGCGGAAGAACTTCGCCAAGCG
TTAGAGAAATTTACCATTGATCTGACGGAACGTGCAGAACAGGGCAAACTTGACCCTGTGATTGGACGTGACGATGAAAT
TCGTCGCACCATCCAAGTATTGCAGCGTCGAACCAAAAACAACCCCGTGATCATTGGTGAGCCAGGTGTTGGTAAAACCG
CCATTGTTGAAGGTTTAGCACAACGTATCATCAATAACGAGGTGCCCGAAGGTCTGCGTGGTCGCCGAGTGTTGTCACTC
GATATGGGCGCGTTGGTGGCTGGAGCGAAGTACCGTGGTGAGTTTGAAGAGCGACTTAAATCGGTATTGAATGAGTTGTC
TAAAGAAGAAGGCAATATCATCCTCTTTATTGATGAGCTTCATACCATGGTTGGCGCCGGTAAAGGTGAAGGTTCGATGG
ATGCGGGCAATATGCTCAAACCCGCATTGGCACGTGGTGAGTTACATTGTGTGGGCGCAACGACACTTGACGAGTATCGT
CAGTATATCGAGAAAGATCCGGCACTTGAGCGCCGCTTCCAAAAAGTGCTGGTTGATGAACCAACAGTGGAAGACACCAT
TGCAATTTTGCGTGGTTTAAAAGAGCGCTATGAGCTGCATCATCATGTTGAAATTACCGATCCAGCGATCGTTGCTGCGG
CCAGTTTGTCGCATCGCTATGTTTCTGATCGTCAATTACCGGATAAAGCGATCGACCTTATCGACGAAGCCGCATCCAGT
ATTCGCATGCAGATCGATTCTAAACCCGAAGCTCTGGATAAGTTGGAGCGCAAAATCATCCAACTTAAGATAGAGCAACA
GGCGCTGAGTAATGAACACGATGAAGCGAGTGAAAAACGTCTGCGCTCACTTAACGAAGAGTTGAATGAGAAAGAGCGTG
AGTTTGCTGAGTTAGAGGAAATTTGGAACGCAGAAAAAGCCGCGCTGTCTGGCACTCAGCACATTAAAGCGGCGTTAGAG
CAAGCACGCATGGATATGGAGTTTGCGAGACGAGCGGGGGATCTTAGCCGCATGTCTGAGCTTCAATATGGCCGTATTCC
AGAGCTTGAGAAGCAACTTGACCTAGCAACGCAAGCAGAAATGCAGGAAATGACCTTGTTGAAAAACAAAGTCACCGATA
ATGAAATTGCAGAAGTGCTTTCTAAGCAAACGGGTATCCCTGTCTCCAAAATGCTTGAAGCAGAAAAAGAAAAACTGCTT
CGCATGGAAGAGGTGCTGCATAAACGTGTTATTGGGCAGAAAGAGGCGGTGGAAGTTGTTGCGAATGCGATACGTCGCAG
CCGGGCGGGCTTGTCCGATCCCAATAAACCGATTGGTTCGTTCTTATTCTTAGGTCCAACCGGTGTGGGGAAAACGGAAC
TGTGCAAAACGTTGGCGAATTTCATGTTCGACAGTGAAGATGCCATGGTGCGTATCGACATGTCTGAGTTTATGGAGAAA
CACTCAGTGGCACGCTTAGTCGGTGCACCTCCGGGCTATGTGGGTTATGAAGAAGGTGGATATTTAACGGAAGCGGTTCG
ACGTAAGCCTTATTCAGTGATTTTGCTCGATGAAGTGGAAAAGGCACACCCTGATGTGTTCAACATCCTGCTACAAGTCT
TGGATGACGGTCGATTGACGGATGGACAAGGTAGAACAGTGGATTTCCGCAATACCGTGGTCATCATGACATCAAACCTA
GGCTCAAGCCGCATCCAAGAAAACTTCGCTATGTTGGATTACCAAGGTATCAAAGAACAGGTGATGGAAGTGGTGACCAA
ACACTTCAGGCCGGAGTTTTTGAACCGTGTTGATGAGACAGTGGTCTTCCACCCACTTAGTCAAGATCATATTAAGTCGA
TCGCAGCGATTCAACTTAACCGCTTGGCGAATCGCATGGAAGAGCATGGCTATCAGTTGGAAGTGTCCGATAAAGCGCTC
GAATTGATCGCACAAGTTGGTTTTGACCCGGTTTACGGAGCAAGACCATTGAAACGAGCGATTCAGCAAAGTATCGAGAA
CCCACTGGCGAAATCTATTTTGGCGGGCTCGGTGCTACCGGATAAGAAAATACAGCTTATCGTCAACAACGACCAAATTG
TGGCACACCAATAG

Upstream 100 bases:

>100_bases
CGAGCGTTAGTTTTTAATTCCCCCTTGAAAAACGATTTTTGCGTAGCCATCTTTCATACTGATTAAAGAATTTACTTCAG
TTGAGGTTAGGAAGGTAGCT

Downstream 100 bases:

>100_bases
ATAGAGTGGTTTTCTCTTCTTTGAGGGAGAAAAATAGTAAATCAAAAGAGGCGAAACGCCTCTTTTGATGTTTTTAAACA
CAACTTGAGCAAAAAGCAGG

Product: clpB protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 857; Mature: 857

Protein sequence:

>857_residues
MRLDRFTSKFQIAISDAQSLALGRDHQYIEPVHLMVALLDQNGSPIRPLLTILNVDVTHLRSKLSEMLDRLPKVSGIGGD
VQLSSAMGAMFNLCDKIAQKRQDAYISSEIFLLAAIEDRGPLGQLFKELGLTEQKVSQAIEQIRGGQKVNDQNAEELRQA
LEKFTIDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRTKNNPVIIGEPGVGKTAIVEGLAQRIINNEVPEGLRGRRVLSL
DMGALVAGAKYRGEFEERLKSVLNELSKEEGNIILFIDELHTMVGAGKGEGSMDAGNMLKPALARGELHCVGATTLDEYR
QYIEKDPALERRFQKVLVDEPTVEDTIAILRGLKERYELHHHVEITDPAIVAAASLSHRYVSDRQLPDKAIDLIDEAASS
IRMQIDSKPEALDKLERKIIQLKIEQQALSNEHDEASEKRLRSLNEELNEKEREFAELEEIWNAEKAALSGTQHIKAALE
QARMDMEFARRAGDLSRMSELQYGRIPELEKQLDLATQAEMQEMTLLKNKVTDNEIAEVLSKQTGIPVSKMLEAEKEKLL
RMEEVLHKRVIGQKEAVEVVANAIRRSRAGLSDPNKPIGSFLFLGPTGVGKTELCKTLANFMFDSEDAMVRIDMSEFMEK
HSVARLVGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSSRIQENFAMLDYQGIKEQVMEVVTKHFRPEFLNRVDETVVFHPLSQDHIKSIAAIQLNRLANRMEEHGYQLEVSDKAL
ELIAQVGFDPVYGARPLKRAIQQSIENPLAKSILAGSVLPDKKIQLIVNNDQIVAHQ

Sequences:

>Translated_857_residues
MRLDRFTSKFQIAISDAQSLALGRDHQYIEPVHLMVALLDQNGSPIRPLLTILNVDVTHLRSKLSEMLDRLPKVSGIGGD
VQLSSAMGAMFNLCDKIAQKRQDAYISSEIFLLAAIEDRGPLGQLFKELGLTEQKVSQAIEQIRGGQKVNDQNAEELRQA
LEKFTIDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRTKNNPVIIGEPGVGKTAIVEGLAQRIINNEVPEGLRGRRVLSL
DMGALVAGAKYRGEFEERLKSVLNELSKEEGNIILFIDELHTMVGAGKGEGSMDAGNMLKPALARGELHCVGATTLDEYR
QYIEKDPALERRFQKVLVDEPTVEDTIAILRGLKERYELHHHVEITDPAIVAAASLSHRYVSDRQLPDKAIDLIDEAASS
IRMQIDSKPEALDKLERKIIQLKIEQQALSNEHDEASEKRLRSLNEELNEKEREFAELEEIWNAEKAALSGTQHIKAALE
QARMDMEFARRAGDLSRMSELQYGRIPELEKQLDLATQAEMQEMTLLKNKVTDNEIAEVLSKQTGIPVSKMLEAEKEKLL
RMEEVLHKRVIGQKEAVEVVANAIRRSRAGLSDPNKPIGSFLFLGPTGVGKTELCKTLANFMFDSEDAMVRIDMSEFMEK
HSVARLVGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSSRIQENFAMLDYQGIKEQVMEVVTKHFRPEFLNRVDETVVFHPLSQDHIKSIAAIQLNRLANRMEEHGYQLEVSDKAL
ELIAQVGFDPVYGARPLKRAIQQSIENPLAKSILAGSVLPDKKIQLIVNNDQIVAHQ
>Mature_857_residues
MRLDRFTSKFQIAISDAQSLALGRDHQYIEPVHLMVALLDQNGSPIRPLLTILNVDVTHLRSKLSEMLDRLPKVSGIGGD
VQLSSAMGAMFNLCDKIAQKRQDAYISSEIFLLAAIEDRGPLGQLFKELGLTEQKVSQAIEQIRGGQKVNDQNAEELRQA
LEKFTIDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRTKNNPVIIGEPGVGKTAIVEGLAQRIINNEVPEGLRGRRVLSL
DMGALVAGAKYRGEFEERLKSVLNELSKEEGNIILFIDELHTMVGAGKGEGSMDAGNMLKPALARGELHCVGATTLDEYR
QYIEKDPALERRFQKVLVDEPTVEDTIAILRGLKERYELHHHVEITDPAIVAAASLSHRYVSDRQLPDKAIDLIDEAASS
IRMQIDSKPEALDKLERKIIQLKIEQQALSNEHDEASEKRLRSLNEELNEKEREFAELEEIWNAEKAALSGTQHIKAALE
QARMDMEFARRAGDLSRMSELQYGRIPELEKQLDLATQAEMQEMTLLKNKVTDNEIAEVLSKQTGIPVSKMLEAEKEKLL
RMEEVLHKRVIGQKEAVEVVANAIRRSRAGLSDPNKPIGSFLFLGPTGVGKTELCKTLANFMFDSEDAMVRIDMSEFMEK
HSVARLVGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSSRIQENFAMLDYQGIKEQVMEVVTKHFRPEFLNRVDETVVFHPLSQDHIKSIAAIQLNRLANRMEEHGYQLEVSDKAL
ELIAQVGFDPVYGARPLKRAIQQSIENPLAKSILAGSVLPDKKIQLIVNNDQIVAHQ

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=300, Percent_Identity=38.6666666666667, Blast_Score=214, Evalue=2e-55,
Organism=Escherichia coli, GI1788943, Length=857, Percent_Identity=76.3127187864644, Blast_Score=1354, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=331, Percent_Identity=42.2960725075529, Blast_Score=273, Evalue=5e-74,
Organism=Saccharomyces cerevisiae, GI6320464, Length=709, Percent_Identity=50.916784203103, Blast_Score=749, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=860, Percent_Identity=41.8604651162791, Blast_Score=671, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 96099; Mature: 96099

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDRFTSKFQIAISDAQSLALGRDHQYIEPVHLMVALLDQNGSPIRPLLTILNVDVTHL
CCCHHHCCHHEEEEHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHH
RSKLSEMLDRLPKVSGIGGDVQLSSAMGAMFNLCDKIAQKRQDAYISSEIFLLAAIEDRG
HHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
PLGQLFKELGLTEQKVSQAIEQIRGGQKVNDQNAEELRQALEKFTIDLTERAEQGKLDPV
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
IGRDDEIRRTIQVLQRRTKNNPVIIGEPGVGKTAIVEGLAQRIINNEVPEGLRGRRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DMGALVAGAKYRGEFEERLKSVLNELSKEEGNIILFIDELHTMVGAGKGEGSMDAGNMLK
CHHHHHHCHHHCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCHHH
PALARGELHCVGATTLDEYRQYIEKDPALERRFQKVLVDEPTVEDTIAILRGLKERYELH
HHHHCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HHVEITDPAIVAAASLSHRYVSDRQLPDKAIDLIDEAASSIRMQIDSKPEALDKLERKII
HCEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHH
QLKIEQQALSNEHDEASEKRLRSLNEELNEKEREFAELEEIWNAEKAALSGTQHIKAALE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHH
QARMDMEFARRAGDLSRMSELQYGRIPELEKQLDLATQAEMQEMTLLKNKVTDNEIAEVL
HHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SKQTGIPVSKMLEAEKEKLLRMEEVLHKRVIGQKEAVEVVANAIRRSRAGLSDPNKPIGS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKTLANFMFDSEDAMVRIDMSEFMEKHSVARLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
HHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSSRIQENFAMLDYQGIKEQVMEVVTKHFRPEFLNRVDETVVFHPLSQDHIKSIAAIQLN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCHHHHHHHHHHHHH
RLANRMEEHGYQLEVSDKALELIAQVGFDPVYGARPLKRAIQQSIENPLAKSILAGSVLP
HHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
DKKIQLIVNNDQIVAHQ
CCCEEEEECCCEEEECC
>Mature Secondary Structure
MRLDRFTSKFQIAISDAQSLALGRDHQYIEPVHLMVALLDQNGSPIRPLLTILNVDVTHL
CCCHHHCCHHEEEEHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHH
RSKLSEMLDRLPKVSGIGGDVQLSSAMGAMFNLCDKIAQKRQDAYISSEIFLLAAIEDRG
HHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
PLGQLFKELGLTEQKVSQAIEQIRGGQKVNDQNAEELRQALEKFTIDLTERAEQGKLDPV
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
IGRDDEIRRTIQVLQRRTKNNPVIIGEPGVGKTAIVEGLAQRIINNEVPEGLRGRRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DMGALVAGAKYRGEFEERLKSVLNELSKEEGNIILFIDELHTMVGAGKGEGSMDAGNMLK
CHHHHHHCHHHCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCHHH
PALARGELHCVGATTLDEYRQYIEKDPALERRFQKVLVDEPTVEDTIAILRGLKERYELH
HHHHCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HHVEITDPAIVAAASLSHRYVSDRQLPDKAIDLIDEAASSIRMQIDSKPEALDKLERKII
HCEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHH
QLKIEQQALSNEHDEASEKRLRSLNEELNEKEREFAELEEIWNAEKAALSGTQHIKAALE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHH
QARMDMEFARRAGDLSRMSELQYGRIPELEKQLDLATQAEMQEMTLLKNKVTDNEIAEVL
HHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SKQTGIPVSKMLEAEKEKLLRMEEVLHKRVIGQKEAVEVVANAIRRSRAGLSDPNKPIGS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKTLANFMFDSEDAMVRIDMSEFMEKHSVARLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
HHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSSRIQENFAMLDYQGIKEQVMEVVTKHFRPEFLNRVDETVVFHPLSQDHIKSIAAIQLN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCHHHHHHHHHHHHH
RLANRMEEHGYQLEVSDKALELIAQVGFDPVYGARPLKRAIQQSIENPLAKSILAGSVLP
HHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
DKKIQLIVNNDQIVAHQ
CCCEEEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]