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The map label for this gene is murD

Identifier: 37678796

GI number: 37678796

Start: 596916

End: 598244

Strand: Direct

Name: murD

Synonym: VV0612

Alternate gene names: 37678796

Gene position: 596916-598244 (Clockwise)

Preceding gene: 37678795

Following gene: 37678797

Centisome position: 17.79

GC content: 48.83

Gene sequence:

>1329_bases
ATGGATCGTTGGCAAGACATCAAACAGGTTGTGGTGGTAGGGCTCGGTATTACTGGGCTCTCCGTCGTTAGACATTTGCG
AAAAACACAGCCGCAGTTGCAAGTGAAAGTGATTGATACGCGTCCAACCCCACCTGGTGTGGAACAATTGCCTCCAGACA
TTGCGCTGCATGTTGGCAGTTGGAATGAAGCTTGGTTAGCTGAGGCTGATTTGGTCGTCACAAACCCAGGCATCGCCTTA
GCGACGCCTCAAATTCAAACGGTGTTAGCACGCGGCGTTGCTGTGGTGGGTGATATCGAGTTGTTCGCTTGGGCGGCAGA
TAAACCCGTCATCGCGATTACTGGCTCAAATGGCAAAAGCACCGTCACGGATTTAACCGGAGTGATGGCTAACGCCTGTG
GGGTGAAATGCGCGATTGGCGGTAACATTGGTGTTCCTGCACTCGATTTGTTGGAGCAAGAGGTTGAGCTTTATGTACTT
GAACTCTCGAGTTTTCAATTAGAGACGACTACCAGTCTCCATTTGGTTGCAGCGGCATTTTTGAACCTTTCTGAAGATCA
CATGGATCGCTACCAAGGCATGGATGACTATCGCCAAGCTAAACTGCGCATCTTTCAGCATGCAAAACATGGTGTCGTGA
ATCGCGATGATCGTCAGACTTATCCTGAAACTTCCCATGGCCAGCAATCACTTGCTTTGGTGACTTTTGGCTCCGATGAC
AAAGAGTTTGGTGTCATGAGTCATCAGGGTGAATCTTGGTTGTCATATAACCAGCAACCTATTTTGGCAAGCCGAGAACT
CAAATTAGTCGGTCAGCACAACGTGGCAAACGTATTGGTGGTTTTGGCGCTGCTGACGTGCGCTGGTATTGATTACCGTA
AAGGCTTGAGCGCTTTGAAGTCTTACACCGGTTTGACACACCGATGTCAGGTTGTCGCAGATAATCGTGGCATCAAGTGG
GTCAATGATTCAAAAGCGACAAATCTTGCTAGTACACAGGCTGCCTTATCGGGGCTTAACTGTGCTGGCAAACTCTACCT
GCTGGTGGGTGGAGATGGCAAAGGGGCTGACTTCTCGCCATTGAAGCCGATTTTGGCGCAGCTAAACCTCCAACTATGTT
GTTTTGGTGCTGATGGTGATCAATTTATGCCATTGCACGCATCGGCCACGCGTTTTGAGCGGATGGAAGATGTGATTGAA
CATATATCTCCACAATTACAATCTGGAGATATGGTGATGCTTTCCCCTGCATGTGCTAGTTTTGACCAATTCAGTAATTT
TATGGCGCGAGGCGATCGATTCGCCGAATTGGCTCGCCAGTATGCATAG

Upstream 100 bases:

>100_bases
TGTGCGTTTCTGGATTATTTCTATGGTGTTGGTTCTGATCGGTCTAGCGACACTGAAAGTGCGTTAATCGACGGACACTC
TTTTTACGGTGTAATGAAAT

Downstream 100 bases:

>100_bases
CGCAACCTCTAACGCGTATTTTTAACAAGGCACGATGTTTGGTGATGGTAGGTAATATCAAAAACAATTTGTTCGGCTGG
TTTCGCCACTCACCACCAGA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MDRWQDIKQVVVVGLGITGLSVVRHLRKTQPQLQVKVIDTRPTPPGVEQLPPDIALHVGSWNEAWLAEADLVVTNPGIAL
ATPQIQTVLARGVAVVGDIELFAWAADKPVIAITGSNGKSTVTDLTGVMANACGVKCAIGGNIGVPALDLLEQEVELYVL
ELSSFQLETTTSLHLVAAAFLNLSEDHMDRYQGMDDYRQAKLRIFQHAKHGVVNRDDRQTYPETSHGQQSLALVTFGSDD
KEFGVMSHQGESWLSYNQQPILASRELKLVGQHNVANVLVVLALLTCAGIDYRKGLSALKSYTGLTHRCQVVADNRGIKW
VNDSKATNLASTQAALSGLNCAGKLYLLVGGDGKGADFSPLKPILAQLNLQLCCFGADGDQFMPLHASATRFERMEDVIE
HISPQLQSGDMVMLSPACASFDQFSNFMARGDRFAELARQYA

Sequences:

>Translated_442_residues
MDRWQDIKQVVVVGLGITGLSVVRHLRKTQPQLQVKVIDTRPTPPGVEQLPPDIALHVGSWNEAWLAEADLVVTNPGIAL
ATPQIQTVLARGVAVVGDIELFAWAADKPVIAITGSNGKSTVTDLTGVMANACGVKCAIGGNIGVPALDLLEQEVELYVL
ELSSFQLETTTSLHLVAAAFLNLSEDHMDRYQGMDDYRQAKLRIFQHAKHGVVNRDDRQTYPETSHGQQSLALVTFGSDD
KEFGVMSHQGESWLSYNQQPILASRELKLVGQHNVANVLVVLALLTCAGIDYRKGLSALKSYTGLTHRCQVVADNRGIKW
VNDSKATNLASTQAALSGLNCAGKLYLLVGGDGKGADFSPLKPILAQLNLQLCCFGADGDQFMPLHASATRFERMEDVIE
HISPQLQSGDMVMLSPACASFDQFSNFMARGDRFAELARQYA
>Mature_442_residues
MDRWQDIKQVVVVGLGITGLSVVRHLRKTQPQLQVKVIDTRPTPPGVEQLPPDIALHVGSWNEAWLAEADLVVTNPGIAL
ATPQIQTVLARGVAVVGDIELFAWAADKPVIAITGSNGKSTVTDLTGVMANACGVKCAIGGNIGVPALDLLEQEVELYVL
ELSSFQLETTTSLHLVAAAFLNLSEDHMDRYQGMDDYRQAKLRIFQHAKHGVVNRDDRQTYPETSHGQQSLALVTFGSDD
KEFGVMSHQGESWLSYNQQPILASRELKLVGQHNVANVLVVLALLTCAGIDYRKGLSALKSYTGLTHRCQVVADNRGIKW
VNDSKATNLASTQAALSGLNCAGKLYLLVGGDGKGADFSPLKPILAQLNLQLCCFGADGDQFMPLHASATRFERMEDVIE
HISPQLQSGDMVMLSPACASFDQFSNFMARGDRFAELARQYA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=437, Percent_Identity=52.1739130434783, Blast_Score=423, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 47978; Mature: 47978

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRWQDIKQVVVVGLGITGLSVVRHLRKTQPQLQVKVIDTRPTPPGVEQLPPDIALHVGS
CCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHCCCCEEEEECC
WNEAWLAEADLVVTNPGIALATPQIQTVLARGVAVVGDIELFAWAADKPVIAITGSNGKS
CCCCCEECCCEEEECCCCEEECHHHHHHHHCCEEEEECEEEEEEECCCCEEEEECCCCCC
TVTDLTGVMANACGVKCAIGGNIGVPALDLLEQEVELYVLELSSFQLETTTSLHLVAAAF
HHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHEEEEEECCEEEECCCHHHHHHHHH
LNLSEDHMDRYQGMDDYRQAKLRIFQHAKHGVVNRDDRQTYPETSHGQQSLALVTFGSDD
HCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCC
KEFGVMSHQGESWLSYNQQPILASRELKLVGQHNVANVLVVLALLTCAGIDYRKGLSALK
CCCCCCCCCCCHHHCCCCCCCEECCCEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
SYTGLTHRCQVVADNRGIKWVNDSKATNLASTQAALSGLNCAGKLYLLVGGDGKGADFSP
HHCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCH
LKPILAQLNLQLCCFGADGDQFMPLHASATRFERMEDVIEHISPQLQSGDMVMLSPACAS
HHHHHHHCCEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHC
FDQFSNFMARGDRFAELARQYA
HHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MDRWQDIKQVVVVGLGITGLSVVRHLRKTQPQLQVKVIDTRPTPPGVEQLPPDIALHVGS
CCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHCCCCEEEEECC
WNEAWLAEADLVVTNPGIALATPQIQTVLARGVAVVGDIELFAWAADKPVIAITGSNGKS
CCCCCEECCCEEEECCCCEEECHHHHHHHHCCEEEEECEEEEEEECCCCEEEEECCCCCC
TVTDLTGVMANACGVKCAIGGNIGVPALDLLEQEVELYVLELSSFQLETTTSLHLVAAAF
HHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHEEEEEECCEEEECCCHHHHHHHHH
LNLSEDHMDRYQGMDDYRQAKLRIFQHAKHGVVNRDDRQTYPETSHGQQSLALVTFGSDD
HCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCC
KEFGVMSHQGESWLSYNQQPILASRELKLVGQHNVANVLVVLALLTCAGIDYRKGLSALK
CCCCCCCCCCCHHHCCCCCCCEECCCEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
SYTGLTHRCQVVADNRGIKWVNDSKATNLASTQAALSGLNCAGKLYLLVGGDGKGADFSP
HHCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCH
LKPILAQLNLQLCCFGADGDQFMPLHASATRFERMEDVIEHISPQLQSGDMVMLSPACAS
HHHHHHHCCEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHC
FDQFSNFMARGDRFAELARQYA
HHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA