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The map label for this gene is murF [H]

Identifier: 37678794

GI number: 37678794

Start: 594442

End: 595803

Strand: Direct

Name: murF [H]

Synonym: VV0610

Alternate gene names: 37678794

Gene position: 594442-595803 (Clockwise)

Preceding gene: 37678793

Following gene: 37678795

Centisome position: 17.72

GC content: 49.49

Gene sequence:

>1362_bases
ATGATTGAGACCTCTCTGAGTCATTTGGCGCAATTGACGCAAGGACAACTGATTGGCGATGACGCCACCTTTTGTACCGT
TTCAACGGATACGCGTCATATCCCTCAGCAAGCGTTGTTTATTGCGCTCGTCGGTGAACGTTTTGATGCGCATGATTTTT
GCCAGCAAGCGGTGGACGCAGGTGCTGTTGCATTGTTGGTGGAGCGAGCGCTGGATCTTCCCGTCGCACAAGTGGTGGTC
AAAGATACGAAAATGGCACTCGGTCAGCTCGGAGCGAATGTGCATCAGCGCTGCCAAACTCCAACCGTGGCGATCACGGG
CAGTTGCGGCAAAACCACGGTGAAAGAGATGGTGGCCAGTATTCTGCAGCAAAAAGGTAAAGTGCTGTTCACGGCAGGTA
ATTTTAACAATGACATTGGTGCGCCTCTGACGTTGCTACGCTCTGAGCCAGAAGATGATTTTGCGGTGATTGAACTGGGC
GCCAATCATATTGGTGAAATTGCCTATACCACCCAGCTTGTTCGGCCTGATGTGGCGTTGGTGAACAACGTGGCTGCGGC
GCATCTTGAAGGGTTTGGCTCGATTGATGGCGTGAAACAAGCGAAAGGGGAGATCTACCAAGGTTTGTCTGCTGGTGGCG
TGGCCATCGTCAATCTAGACAGTCATGGCGGTGAGTACTGGACACAGGTACTGGCCGATAAACAGGTCACGACGTTTTCG
GCAAGCTCCGAGCAGGCTGATTTCTACGCAACAGACATCGTACTGGATACGCAAGGGAAAGCGTCATTCGTATTGCATAC
GCCTGAAGAACGCATTCCACTTTCTCTTGGTATCATTGGCCAACATAACGTCGCCAATGCGATTGCCGCTAGCATCATTG
CTTTGCAGTTTGGTGCCAGCTTAGCGCAAATCCAATCGGGATTGCTCAATTTGATGCCTGTCAAAGGGCGTGTTGATGTT
CAACAATTATCCGACAACATCAAACTGATTGATGACAGTTATAATGCCAGTGTGCCAGCGATGAAGGCGGCGGTGGACCT
ATTAGGTAGTTTTACCGGACAGCGTTGGTTAGTTTTAGGTTATATGGCGGAATTGGGCAATGAAAGTCTTGCACTTCATC
GTCAAGTCGGTGAACATGCTGCCCCATTAAATTTTGAACACGTCCTTACCTATGGCGAGGATACAAGGATCATTAGCCAG
TTGTGTGGTGGAAAGCATTTTTCAGACCATGGTGAACTGATCGAATACATCAAGCAGAATTTGGCCAACGAAAATGAAGC
CTGTCATACCGTGTTGGTCAAAGGCGCAAACTCAGCTGGCATGAGTAAAGTGGCTGCTGCTTTGAAGGAGAACTATCGAT
GA

Upstream 100 bases:

>100_bases
TGTTGGCAGGCAAAGGCCACGAAGATTATCAAGTACTCAAGCACCAGACGGTTCACTACTCGGATCGTGAATCGGCGCAG
CAACTGCTAGGAATTTCATC

Downstream 100 bases:

>100_bases
TTATTTGGCTTGCCGAGCTGCTACAGCCATACTTTTCCTTTTTCCGACTGTTTGAATACCTGTCGTTTCGCGCGATCTTA
AGTGTGCTAACCGCGTTGGG

Product: UDP-N-acetylmuramyl pentapeptide synthase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MIETSLSHLAQLTQGQLIGDDATFCTVSTDTRHIPQQALFIALVGERFDAHDFCQQAVDAGAVALLVERALDLPVAQVVV
KDTKMALGQLGANVHQRCQTPTVAITGSCGKTTVKEMVASILQQKGKVLFTAGNFNNDIGAPLTLLRSEPEDDFAVIELG
ANHIGEIAYTTQLVRPDVALVNNVAAAHLEGFGSIDGVKQAKGEIYQGLSAGGVAIVNLDSHGGEYWTQVLADKQVTTFS
ASSEQADFYATDIVLDTQGKASFVLHTPEERIPLSLGIIGQHNVANAIAASIIALQFGASLAQIQSGLLNLMPVKGRVDV
QQLSDNIKLIDDSYNASVPAMKAAVDLLGSFTGQRWLVLGYMAELGNESLALHRQVGEHAAPLNFEHVLTYGEDTRIISQ
LCGGKHFSDHGELIEYIKQNLANENEACHTVLVKGANSAGMSKVAAALKENYR

Sequences:

>Translated_453_residues
MIETSLSHLAQLTQGQLIGDDATFCTVSTDTRHIPQQALFIALVGERFDAHDFCQQAVDAGAVALLVERALDLPVAQVVV
KDTKMALGQLGANVHQRCQTPTVAITGSCGKTTVKEMVASILQQKGKVLFTAGNFNNDIGAPLTLLRSEPEDDFAVIELG
ANHIGEIAYTTQLVRPDVALVNNVAAAHLEGFGSIDGVKQAKGEIYQGLSAGGVAIVNLDSHGGEYWTQVLADKQVTTFS
ASSEQADFYATDIVLDTQGKASFVLHTPEERIPLSLGIIGQHNVANAIAASIIALQFGASLAQIQSGLLNLMPVKGRVDV
QQLSDNIKLIDDSYNASVPAMKAAVDLLGSFTGQRWLVLGYMAELGNESLALHRQVGEHAAPLNFEHVLTYGEDTRIISQ
LCGGKHFSDHGELIEYIKQNLANENEACHTVLVKGANSAGMSKVAAALKENYR
>Mature_453_residues
MIETSLSHLAQLTQGQLIGDDATFCTVSTDTRHIPQQALFIALVGERFDAHDFCQQAVDAGAVALLVERALDLPVAQVVV
KDTKMALGQLGANVHQRCQTPTVAITGSCGKTTVKEMVASILQQKGKVLFTAGNFNNDIGAPLTLLRSEPEDDFAVIELG
ANHIGEIAYTTQLVRPDVALVNNVAAAHLEGFGSIDGVKQAKGEIYQGLSAGGVAIVNLDSHGGEYWTQVLADKQVTTFS
ASSEQADFYATDIVLDTQGKASFVLHTPEERIPLSLGIIGQHNVANAIAASIIALQFGASLAQIQSGLLNLMPVKGRVDV
QQLSDNIKLIDDSYNASVPAMKAAVDLLGSFTGQRWLVLGYMAELGNESLALHRQVGEHAAPLNFEHVLTYGEDTRIISQ
LCGGKHFSDHGELIEYIKQNLANENEACHTVLVKGANSAGMSKVAAALKENYR

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=452, Percent_Identity=50.4424778761062, Blast_Score=390, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48336; Mature: 48336

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIETSLSHLAQLTQGQLIGDDATFCTVSTDTRHIPQQALFIALVGERFDAHDFCQQAVDA
CCCCHHHHHHHHHCCCEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCC
GAVALLVERALDLPVAQVVVKDTKMALGQLGANVHQRCQTPTVAITGSCGKTTVKEMVAS
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHCCCCCEEEECCCCHHHHHHHHHH
ILQQKGKVLFTAGNFNNDIGAPLTLLRSEPEDDFAVIELGANHIGEIAYTTQLVRPDVAL
HHHHCCCEEEEECCCCCCCCCCHHEECCCCCCCEEEEEECCHHHHHHHHHHHHHCCCHHH
VNNVAAAHLEGFGSIDGVKQAKGEIYQGLSAGGVAIVNLDSHGGEYWTQVLADKQVTTFS
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCEEEEE
ASSEQADFYATDIVLDTQGKASFVLHTPEERIPLSLGIIGQHNVANAIAASIIALQFGAS
CCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCH
LAQIQSGLLNLMPVKGRVDVQQLSDNIKLIDDSYNASVPAMKAAVDLLGSFTGQRWLVLG
HHHHHHCHHHEECCCCCCCHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHH
YMAELGNESLALHRQVGEHAAPLNFEHVLTYGEDTRIISQLCGGKHFSDHGELIEYIKQN
HHHHHCCCHHHHHHHHCCCCCCCCHHHEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
LANENEACHTVLVKGANSAGMSKVAAALKENYR
HCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MIETSLSHLAQLTQGQLIGDDATFCTVSTDTRHIPQQALFIALVGERFDAHDFCQQAVDA
CCCCHHHHHHHHHCCCEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCC
GAVALLVERALDLPVAQVVVKDTKMALGQLGANVHQRCQTPTVAITGSCGKTTVKEMVAS
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHCCCCCEEEECCCCHHHHHHHHHH
ILQQKGKVLFTAGNFNNDIGAPLTLLRSEPEDDFAVIELGANHIGEIAYTTQLVRPDVAL
HHHHCCCEEEEECCCCCCCCCCHHEECCCCCCCEEEEEECCHHHHHHHHHHHHHCCCHHH
VNNVAAAHLEGFGSIDGVKQAKGEIYQGLSAGGVAIVNLDSHGGEYWTQVLADKQVTTFS
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCEEEEE
ASSEQADFYATDIVLDTQGKASFVLHTPEERIPLSLGIIGQHNVANAIAASIIALQFGAS
CCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCH
LAQIQSGLLNLMPVKGRVDVQQLSDNIKLIDDSYNASVPAMKAAVDLLGSFTGQRWLVLG
HHHHHHCHHHEECCCCCCCHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHH
YMAELGNESLALHRQVGEHAAPLNFEHVLTYGEDTRIISQLCGGKHFSDHGELIEYIKQN
HHHHHCCCHHHHHHHHCCCCCCCCHHHEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
LANENEACHTVLVKGANSAGMSKVAAALKENYR
HCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2668880; 8973200; 1630901; 9278503; 9166795; 2186811; 11090285 [H]