Click here to switch to the map view.

The map label for this gene is murE

Identifier: 37678793

GI number: 37678793

Start: 592898

End: 594445

Strand: Direct

Name: murE

Synonym: VV0609

Alternate gene names: 37678793

Gene position: 592898-594445 (Clockwise)

Preceding gene: 37678792

Following gene: 37678794

Centisome position: 17.67

GC content: 50.97

Gene sequence:

>1548_bases
ATGTGGCACCCGATGAAAATGCATTCCAGAACTAGTTTTATGGCTGAGCGTGGCTCGGAGAACAAAATGCGAAATACCAT
GAATCTGACAAATTTATTAGCGCCGTGGCTAGATTGTCCTGAGCTTGCGGACATTACCGTCCAATCGCTGGAGTTAGACA
GTCGTCAGGTGAAACAAGGCGATACTTTTGTCGCTATCGTTGGTCACGTGGTGGATGGCCGCCAGTATATTGAGAAAGCA
ATAGAACAGGGCGCAAATGCCGTCATTGCTCAATCTTGTCAGCAATACCCTCCCGGCTTGGTGCGCTATCAGCAAAACGT
GGTGATTGTCTATCTTGAGAAGCTGGACGAAAAACTGTCGCAATTAGCGGGACGCCTGTACCAACATCCTGAGATGTCAT
TAATTGGTGTGACAGGAACCAATGGCAAAACCACCATCACTCAATTGATTGCGCAATGGCTAGAGTTAGCCGGGCAAAAA
GCCGCAGTGATGGGGACGACGGGCAACGGTTTTCTCAATGCCTTACAGCCTGCGGCCAATACCACGGGCAATGCGGTTGA
GATTCAAAAAACGTTGGCAGACTTACAGCAGCAAGGGGCAAAAGCAACTGCGTTGGAAGTCTCTTCTCATGGCTTAGTGC
AGGGGCGCGTTAAGGCGCTACAGTTTGCCGCTGGCGTGTTTACCAATCTGAGCCGTGATCATTTGGATTATCACGGCACG
ATGGAAGCCTATGCCAAAGCCAAAATGACGCTGTTTACTGAGCACCAGTGCCAGCATGCAATCATCAATCTGGATGACGA
AGTCGGTGCGCAGTGGTTTCAAGAGCTGCAACAAGGGGTCGGCGTTTCATTGTTTCCACAAGATGCAAGTGTAAAAGCAC
TGTGGGCCAGCTCGGTAGCTTACGCAGAAAGCGGTATTACGATTGAGTTTGATGGCTGCTTTGGCCAAGGGCGTCTGCAC
GCGCCATTGATTGGTGAATTTAATGCCACCAACTTACTGCTCGCGTTGGCAACACTGCTAGCCTTAGGCGTTGATAAGCA
AGCTTTATTGGATTCGGCCGCAAGCCTGCGCCCAGTACTTGGACGTATGGAACTGTTTCAAGTGAACAGCAAAGCCAAAG
TGGTGGTCGATTATGCCCATACCCCAGATGCGCTGGAGAAAGCGCTGCAAGCACTGCGTGTTCACTGTACCGGTCATTTG
TGGGCGATTTTCGGCTGTGGTGGTGATCGCGATAAAGGCAAGCGACCAATGATGGCAGAGATTGCAGAGCGTCTGGCCGA
TCACGTCGTGCTTACCGACGACAACCCACGCAGTGAAGACCCCGCCATGATTGTGCAAGATATGCTGGCGGGCTTAACTC
GTGGAGACAGTGCAGTGGTTGAGCACGATCGCTTCAGTGCGCTTCAATACGCGCTCGACAATGCACAAGCGGATGACATC
ATTTTGTTGGCAGGCAAAGGCCACGAAGATTATCAAGTACTCAAGCACCAGACGGTTCACTACTCGGATCGTGAATCGGC
GCAGCAACTGCTAGGAATTTCATCATGA

Upstream 100 bases:

>100_bases
GTCGTGGTGGTCAATGAACCACAAGGGGATGATTACTATGGCGGTTCCGTCGCTTCCCCTGTTTTTTCAGAAATTATGAA
AGGTGCGCTGCAAATTTTGA

Downstream 100 bases:

>100_bases
TTGAGACCTCTCTGAGTCATTTGGCGCAATTGACGCAAGGACAACTGATTGGCGATGACGCCACCTTTTGTACCGTTTCA
ACGGATACGCGTCATATCCC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MWHPMKMHSRTSFMAERGSENKMRNTMNLTNLLAPWLDCPELADITVQSLELDSRQVKQGDTFVAIVGHVVDGRQYIEKA
IEQGANAVIAQSCQQYPPGLVRYQQNVVIVYLEKLDEKLSQLAGRLYQHPEMSLIGVTGTNGKTTITQLIAQWLELAGQK
AAVMGTTGNGFLNALQPAANTTGNAVEIQKTLADLQQQGAKATALEVSSHGLVQGRVKALQFAAGVFTNLSRDHLDYHGT
MEAYAKAKMTLFTEHQCQHAIINLDDEVGAQWFQELQQGVGVSLFPQDASVKALWASSVAYAESGITIEFDGCFGQGRLH
APLIGEFNATNLLLALATLLALGVDKQALLDSAASLRPVLGRMELFQVNSKAKVVVDYAHTPDALEKALQALRVHCTGHL
WAIFGCGGDRDKGKRPMMAEIAERLADHVVLTDDNPRSEDPAMIVQDMLAGLTRGDSAVVEHDRFSALQYALDNAQADDI
ILLAGKGHEDYQVLKHQTVHYSDRESAQQLLGISS

Sequences:

>Translated_515_residues
MWHPMKMHSRTSFMAERGSENKMRNTMNLTNLLAPWLDCPELADITVQSLELDSRQVKQGDTFVAIVGHVVDGRQYIEKA
IEQGANAVIAQSCQQYPPGLVRYQQNVVIVYLEKLDEKLSQLAGRLYQHPEMSLIGVTGTNGKTTITQLIAQWLELAGQK
AAVMGTTGNGFLNALQPAANTTGNAVEIQKTLADLQQQGAKATALEVSSHGLVQGRVKALQFAAGVFTNLSRDHLDYHGT
MEAYAKAKMTLFTEHQCQHAIINLDDEVGAQWFQELQQGVGVSLFPQDASVKALWASSVAYAESGITIEFDGCFGQGRLH
APLIGEFNATNLLLALATLLALGVDKQALLDSAASLRPVLGRMELFQVNSKAKVVVDYAHTPDALEKALQALRVHCTGHL
WAIFGCGGDRDKGKRPMMAEIAERLADHVVLTDDNPRSEDPAMIVQDMLAGLTRGDSAVVEHDRFSALQYALDNAQADDI
ILLAGKGHEDYQVLKHQTVHYSDRESAQQLLGISS
>Mature_515_residues
MWHPMKMHSRTSFMAERGSENKMRNTMNLTNLLAPWLDCPELADITVQSLELDSRQVKQGDTFVAIVGHVVDGRQYIEKA
IEQGANAVIAQSCQQYPPGLVRYQQNVVIVYLEKLDEKLSQLAGRLYQHPEMSLIGVTGTNGKTTITQLIAQWLELAGQK
AAVMGTTGNGFLNALQPAANTTGNAVEIQKTLADLQQQGAKATALEVSSHGLVQGRVKALQFAAGVFTNLSRDHLDYHGT
MEAYAKAKMTLFTEHQCQHAIINLDDEVGAQWFQELQQGVGVSLFPQDASVKALWASSVAYAESGITIEFDGCFGQGRLH
APLIGEFNATNLLLALATLLALGVDKQALLDSAASLRPVLGRMELFQVNSKAKVVVDYAHTPDALEKALQALRVHCTGHL
WAIFGCGGDRDKGKRPMMAEIAERLADHVVLTDDNPRSEDPAMIVQDMLAGLTRGDSAVVEHDRFSALQYALDNAQADDI
ILLAGKGHEDYQVLKHQTVHYSDRESAQQLLGISS

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=491, Percent_Identity=54.1751527494908, Blast_Score=477, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 56222; Mature: 56222

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWHPMKMHSRTSFMAERGSENKMRNTMNLTNLLAPWLDCPELADITVQSLELDSRQVKQG
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHCCCHHHHHCC
DTFVAIVGHVVDGRQYIEKAIEQGANAVIAQSCQQYPPGLVRYQQNVVIVYLEKLDEKLS
CEEEEEEHHHHCCHHHHHHHHHCCCCHHHHHHHHHCCCHHHEECCCEEEEEHHHHHHHHH
QLAGRLYQHPEMSLIGVTGTNGKTTITQLIAQWLELAGQKAAVMGTTGNGFLNALQPAAN
HHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCC
TTGNAVEIQKTLADLQQQGAKATALEVSSHGLVQGRVKALQFAAGVFTNLSRDHLDYHGT
CCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCCH
MEAYAKAKMTLFTEHQCQHAIINLDDEVGAQWFQELQQGVGVSLFPQDASVKALWASSVA
HHHHHHHHHHEEEHHCCCHHEECCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHH
YAESGITIEFDGCFGQGRLHAPLIGEFNATNLLLALATLLALGVDKQALLDSAASLRPVL
HHCCCCEEEECCCCCCCEECCCEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
GRMELFQVNSKAKVVVDYAHTPDALEKALQALRVHCTGHLWAIFGCGGDRDKGKRPMMAE
CCHHHEEECCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHH
IAERLADHVVLTDDNPRSEDPAMIVQDMLAGLTRGDSAVVEHDRFSALQYALDNAQADDI
HHHHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCE
ILLAGKGHEDYQVLKHQTVHYSDRESAQQLLGISS
EEEECCCCCHHHHHHHHHCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MWHPMKMHSRTSFMAERGSENKMRNTMNLTNLLAPWLDCPELADITVQSLELDSRQVKQG
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHCCCHHHHHCC
DTFVAIVGHVVDGRQYIEKAIEQGANAVIAQSCQQYPPGLVRYQQNVVIVYLEKLDEKLS
CEEEEEEHHHHCCHHHHHHHHHCCCCHHHHHHHHHCCCHHHEECCCEEEEEHHHHHHHHH
QLAGRLYQHPEMSLIGVTGTNGKTTITQLIAQWLELAGQKAAVMGTTGNGFLNALQPAAN
HHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCC
TTGNAVEIQKTLADLQQQGAKATALEVSSHGLVQGRVKALQFAAGVFTNLSRDHLDYHGT
CCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCCH
MEAYAKAKMTLFTEHQCQHAIINLDDEVGAQWFQELQQGVGVSLFPQDASVKALWASSVA
HHHHHHHHHHEEEHHCCCHHEECCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHH
YAESGITIEFDGCFGQGRLHAPLIGEFNATNLLLALATLLALGVDKQALLDSAASLRPVL
HHCCCCEEEECCCCCCCEECCCEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
GRMELFQVNSKAKVVVDYAHTPDALEKALQALRVHCTGHLWAIFGCGGDRDKGKRPMMAE
CCHHHEEECCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHH
IAERLADHVVLTDDNPRSEDPAMIVQDMLAGLTRGDSAVVEHDRFSALQYALDNAQADDI
HHHHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCE
ILLAGKGHEDYQVLKHQTVHYSDRESAQQLLGISS
EEEECCCCCHHHHHHHHHCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA