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The map label for this gene is mlaE [H]

Identifier: 37678640

GI number: 37678640

Start: 443941

End: 444729

Strand: Direct

Name: mlaE [H]

Synonym: VV0456

Alternate gene names: 37678640

Gene position: 443941-444729 (Clockwise)

Preceding gene: 37678639

Following gene: 37678641

Centisome position: 13.23

GC content: 50.82

Gene sequence:

>789_bases
ATGCCTAACTTAGTCGACATGATTTCAGTGCTCGGGCGTCGCTTCCTTAGCTTATGTCAATCATTTGGTAAGGCGAGCTT
AATGCTTGCCGGTGCACTTGCGGGGAGACCACAACCGCGAAAAACGCTGCCACTATTGGTAAAGCAAATCTATAACGTGG
GGGTGCAGTCTTTAGCCATCATCGTGGTCTCTGGACTCTTCATTGGTATGGTGTTGAGTCTACAAGGCTACGTGATTTTG
GTGGATTACGGCGCAGAAGGCAGCCTAGGGCAAATGGTCGCGCTGTCGTTATTGCGTGAGCTAGGGCCAGTTGTGACGGC
TTTGTTGTTTGCTGGGCGAGCGGGCTCTGCGCTCACGGCTGAAATTGGCCTGATGAAAGCGACAGAGCAACTTTCTAGTA
TGGAAATGATGGCGGTTGACCCACTTAAAAGAATCATAGCGCCAAGATTGTGGGCAGGCATTATCTCAATGCCGCTGTTG
GCGATGATCTTCATGGCCGTGGGTATTTGGGGCGGCCAATTGGTGGGTGTTGACTGGAAAGGCATCGACCACGGCAGTTT
TTGGTCAGCCATGCAAGCTTCGGTGGAGTTAGGGCAAGATATTGGTAACAGCATGATCAAATGCGTGGTCTTTGCCATTA
CCGTGACTTGGATTGCGCTCTTTAATGGTTACGATGCTATTCCAACTTCGGAAGGCATCAGCCGTGCGACGACTCGCACG
GTGGTTCACTCTTCTTTAGCGGTGTTGGGGCTGGATTTTGTCCTCACTGCGTTGATGTTTGGGAATTAA

Upstream 100 bases:

>100_bases
AACAGCGATCCTCGTGTTCAACAATTTTTATTGGGTGAAGCGGATGGTCCTGTGCCGTTTCGTTTTCCCGCGCAACCCAT
AGAAAAGGATTTATTTCACG

Downstream 100 bases:

>100_bases
ACATGCAACAAACACGTAAAACAGAATTATGGGTTGGCAGCTTTGTCTTGGCGGGAATTTGCGCAATTCTGGTCATGATT
TTTCAAGTCGCTGACGTAAA

Product: ABC-type transport system, permease component

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MPNLVDMISVLGRRFLSLCQSFGKASLMLAGALAGRPQPRKTLPLLVKQIYNVGVQSLAIIVVSGLFIGMVLSLQGYVIL
VDYGAEGSLGQMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKATEQLSSMEMMAVDPLKRIIAPRLWAGIISMPLL
AMIFMAVGIWGGQLVGVDWKGIDHGSFWSAMQASVELGQDIGNSMIKCVVFAITVTWIALFNGYDAIPTSEGISRATTRT
VVHSSLAVLGLDFVLTALMFGN

Sequences:

>Translated_262_residues
MPNLVDMISVLGRRFLSLCQSFGKASLMLAGALAGRPQPRKTLPLLVKQIYNVGVQSLAIIVVSGLFIGMVLSLQGYVIL
VDYGAEGSLGQMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKATEQLSSMEMMAVDPLKRIIAPRLWAGIISMPLL
AMIFMAVGIWGGQLVGVDWKGIDHGSFWSAMQASVELGQDIGNSMIKCVVFAITVTWIALFNGYDAIPTSEGISRATTRT
VVHSSLAVLGLDFVLTALMFGN
>Mature_261_residues
PNLVDMISVLGRRFLSLCQSFGKASLMLAGALAGRPQPRKTLPLLVKQIYNVGVQSLAIIVVSGLFIGMVLSLQGYVILV
DYGAEGSLGQMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKATEQLSSMEMMAVDPLKRIIAPRLWAGIISMPLLA
MIFMAVGIWGGQLVGVDWKGIDHGSFWSAMQASVELGQDIGNSMIKCVVFAITVTWIALFNGYDAIPTSEGISRATTRTV
VHSSLAVLGLDFVLTALMFGN

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=259, Percent_Identity=73.7451737451737, Blast_Score=382, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27847; Mature: 27715

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNLVDMISVLGRRFLSLCQSFGKASLMLAGALAGRPQPRKTLPLLVKQIYNVGVQSLAI
CCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
IVVSGLFIGMVLSLQGYVILVDYGAEGSLGQMVALSLLRELGPVVTALLFAGRAGSALTA
HHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
EIGLMKATEQLSSMEMMAVDPLKRIIAPRLWAGIISMPLLAMIFMAVGIWGGQLVGVDWK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
GIDHGSFWSAMQASVELGQDIGNSMIKCVVFAITVTWIALFNGYDAIPTSEGISRATTRT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
VVHSSLAVLGLDFVLTALMFGN
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PNLVDMISVLGRRFLSLCQSFGKASLMLAGALAGRPQPRKTLPLLVKQIYNVGVQSLAI
CHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
IVVSGLFIGMVLSLQGYVILVDYGAEGSLGQMVALSLLRELGPVVTALLFAGRAGSALTA
HHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
EIGLMKATEQLSSMEMMAVDPLKRIIAPRLWAGIISMPLLAMIFMAVGIWGGQLVGVDWK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
GIDHGSFWSAMQASVELGQDIGNSMIKCVVFAITVTWIALFNGYDAIPTSEGISRATTRT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
VVHSSLAVLGLDFVLTALMFGN
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]