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The map label for this gene is bioH [H]

Identifier: 37678415

GI number: 37678415

Start: 238607

End: 239455

Strand: Direct

Name: bioH [H]

Synonym: VV0230

Alternate gene names: 37678415

Gene position: 238607-239455 (Clockwise)

Preceding gene: 37678414

Following gene: 37678417

Centisome position: 7.11

GC content: 55.48

Gene sequence:

>849_bases
GTGGGGCGAATGAATCGTGTCGCTAGCGTACTCGACCACATGTGGCGCTTGAGGCTGGAAAAAGTAGGAGTAAGAGGGAA
GATGAGCACAGATTTACACTGGCAAACACAAGGTCAGGGGCCCGATTTGGTGTTGCTGCATGGCTGGGGCATGAATGGCG
CGGTGTGGCAGCAGGTGGTTGAAAGGCTGGAGCCGCACTTTCGTTTGCATGTGGTGGATCTGCCTGGCTATGGCCACAGT
GCCCATCTTCATGCGGCAAGTTTAGAAGAGATTGCCCAGCAGCTTCTCGAGCATGCACCGAAACAAGCTATTTGGGTTGG
CTGGTCATTAGGCGGGCTGGTGGCGACGCACATGGCGCTGCATCACGCCGATTACGTCAGCAAGTTAGTGACGGTGGCGA
GCTCACCGAAGTTCGCTGCAGAAGCGCGTTGGCGTGGCATTCAGCCACAAGTATTGAGCGCCTTTACCGAGCAGTTGAGT
GAAGATTTTCACATCACGGTAGAGCGTTTTATGGCGCTGCAAGCCATGGGCAGCCCTTCTGCACGACAAGATGTGAAAAA
CCTCAAACAAGCGGTGTTCTCGCGCCCGCAACCCAACCCCCAATCACTTTTGGCTGGGCTGCAAATGCTGGCGGAGGTGG
ACTTGCGTGACCATCTGCCGCACTTAACGATGCCGATGTTGCGTCTGTATGGCCGTTTGGACGGGTTGGTGCCGATCAAG
GTGGCGCAGGATCTCGAGAAAGCGTTACCCGCCAGTGAGCAGTTCATTTTCACCCAGTCGTCACACGCGCCTTTTATCAC
TGAACCAGAGAGCTTTTGCCACCAGTTGCTGAGCTTTGCCGGCAAGTAA

Upstream 100 bases:

>100_bases
CGGGTGATGGTCGTGATTCATCGGCAAGCCGCACAGATGACAGAGGCGAGGAAGAGATCGGAGTATTTTTTCACTAAGCC
AATCGGTTAACATACGGGCA

Downstream 100 bases:

>100_bases
GCGAAAAATAAAATTAAACCTGTTCAAATATTGGTCGATACAAGAAGTAACCAAGGCAACTTACTACGGTTAGTGAAGCG
GTTTTGGGGCTGTGCAGTGA

Product: biotin biosynthesis protein BioH

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MGRMNRVASVLDHMWRLRLEKVGVRGKMSTDLHWQTQGQGPDLVLLHGWGMNGAVWQQVVERLEPHFRLHVVDLPGYGHS
AHLHAASLEEIAQQLLEHAPKQAIWVGWSLGGLVATHMALHHADYVSKLVTVASSPKFAAEARWRGIQPQVLSAFTEQLS
EDFHITVERFMALQAMGSPSARQDVKNLKQAVFSRPQPNPQSLLAGLQMLAEVDLRDHLPHLTMPMLRLYGRLDGLVPIK
VAQDLEKALPASEQFIFTQSSHAPFITEPESFCHQLLSFAGK

Sequences:

>Translated_282_residues
MGRMNRVASVLDHMWRLRLEKVGVRGKMSTDLHWQTQGQGPDLVLLHGWGMNGAVWQQVVERLEPHFRLHVVDLPGYGHS
AHLHAASLEEIAQQLLEHAPKQAIWVGWSLGGLVATHMALHHADYVSKLVTVASSPKFAAEARWRGIQPQVLSAFTEQLS
EDFHITVERFMALQAMGSPSARQDVKNLKQAVFSRPQPNPQSLLAGLQMLAEVDLRDHLPHLTMPMLRLYGRLDGLVPIK
VAQDLEKALPASEQFIFTQSSHAPFITEPESFCHQLLSFAGK
>Mature_281_residues
GRMNRVASVLDHMWRLRLEKVGVRGKMSTDLHWQTQGQGPDLVLLHGWGMNGAVWQQVVERLEPHFRLHVVDLPGYGHSA
HLHAASLEEIAQQLLEHAPKQAIWVGWSLGGLVATHMALHHADYVSKLVTVASSPKFAAEARWRGIQPQVLSAFTEQLSE
DFHITVERFMALQAMGSPSARQDVKNLKQAVFSRPQPNPQSLLAGLQMLAEVDLRDHLPHLTMPMLRLYGRLDGLVPIKV
AQDLEKALPASEQFIFTQSSHAPFITEPESFCHQLLSFAGK

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=251, Percent_Identity=53.7848605577689, Blast_Score=274, Evalue=4e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 31564; Mature: 31433

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRMNRVASVLDHMWRLRLEKVGVRGKMSTDLHWQTQGQGPDLVLLHGWGMNGAVWQQVV
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEEECCCCCHHHHHHHH
ERLEPHFRLHVVDLPGYGHSAHLHAASLEEIAQQLLEHAPKQAIWVGWSLGGLVATHMAL
HHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
HHADYVSKLVTVASSPKFAAEARWRGIQPQVLSAFTEQLSEDFHITVERFMALQAMGSPS
HHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCH
ARQDVKNLKQAVFSRPQPNPQSLLAGLQMLAEVDLRDHLPHLTMPMLRLYGRLDGLVPIK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHH
VAQDLEKALPASEQFIFTQSSHAPFITEPESFCHQLLSFAGK
HHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
GRMNRVASVLDHMWRLRLEKVGVRGKMSTDLHWQTQGQGPDLVLLHGWGMNGAVWQQVV
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEEECCCCCHHHHHHHH
ERLEPHFRLHVVDLPGYGHSAHLHAASLEEIAQQLLEHAPKQAIWVGWSLGGLVATHMAL
HHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
HHADYVSKLVTVASSPKFAAEARWRGIQPQVLSAFTEQLSEDFHITVERFMALQAMGSPS
HHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCH
ARQDVKNLKQAVFSRPQPNPQSLLAGLQMLAEVDLRDHLPHLTMPMLRLYGRLDGLVPIK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHH
VAQDLEKALPASEQFIFTQSSHAPFITEPESFCHQLLSFAGK
HHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA