Click here to switch to the map view.

The map label for this gene is btuB

Identifier: 37678350

GI number: 37678350

Start: 173539

End: 175380

Strand: Direct

Name: btuB

Synonym: VV0166

Alternate gene names: 37678350

Gene position: 173539-175380 (Clockwise)

Preceding gene: 37678349

Following gene: 37678351

Centisome position: 5.17

GC content: 44.57

Gene sequence:

>1842_bases
ATGCAAAAATCTCTTTTAGCGATCGCCATGGCATCGCTGCTTACCCCTATCTCTTATTTACACGCTCAAGAAGTGCAAAC
CAATGACACTGTTGTCGTGACGGCCAACCGTTTTGAGCAAGTTGAATCTTCCGTTTTAGCGTCGATTAGCGTGGTGACGA
AGGCTGAAATCGAGCAACTGAATGTGACCACTGCGCTTGATATTTTGAAAACACTTCCCGGTGTGGAGGTAAATAGTCAA
GGTGGTAAAGGGCAAATCAGCAGCATCTTTTTACGCGGTACTTCATCAAAACACACTCTAGTGCTCGTTGATGGTGTCAA
AATTAACTCAGCAACAGCAGGTGGTGCATCATTAGGCTTAATCCCAGCTTTCGCTATTGAACAAATTGAAGTGGTGCGCG
GGCCTCGAGCGGCAATTTATGGTTCAGATGCAATTGGTGGCGTCATTCATATCAAGACTATCCCAGCAAACCGCCAAACT
AAGCATGACGCGAACTTGGGTTATGGCAATGATGACCATTCATCATTGGCATGGCGATCTTCAGGACAGTTAAATGATTC
AACTCAGGCTTCTTTTGTCTTTTCCGATGAAAAAAGTGATGGTTATCGAGTCAATGAAGTTGCACCAAGTGGTGATAGCC
ACGGTTATCAATCTCAAACTTTGTTTGGTTCACTTCGTCATGAGATCGACGACGCTTGGTCTGTTCAGTTTAATGGTTAT
CAGCTGAGTTCAGACGTTGAATACGCAAACCAGTTTTCAGGCGTGAAGAATGAGAGCAATACCGATTTTTATAGCATTGC
TGGTTCATTAAATTTCCATAAAGACAATTATTCTTCTCAGTTGATGGTGTCACGTAGTGATAATGAATCGTGGGATGGTG
TCGCCAGCGGTATGGTTGCTAAAACCGCGCTTTTCTCCAGCCGAAATTCAGTCTCGTGGCTGAACCATTGGGTCGTTGTG
CCCGCACTCACGTTAGCGGCTGGCGTGGATTATGATCAAGAGAATGCGCGCCAAGGCGGCGCGAATGCCACAAATTACAG
CAAAACGGAAAAAGACAATAAAGCCGCATATGTAACGGCTCATTTCTCTAAGAACATTGTGACTGCTGAAGCCAGTATTC
GCTCTGATGATGATAGCGCCTTTGGTAAACACAACACTTGGAATCTAGGCTTAGGTATTGCGCCATCTGATTATGTTGAG
TTTATAGCTTCAACGGGCACAGGTTTCAAAGCTCCAACATTTAACGATCTATATTGGCCAAGTTCAGGTAATCCAAACTT
GAAGCCAGAAACCTCAAAGTCCTCAGAAGTAGGGATTCGCAGCTATTTGCCTTTTGTTCAAATTGATATTTCAGCATACA
GAAACGAAATTGAAGATATGATCGATTGGGCCCCTACAGGACCTGGAGGTGCTTGGACCCCTTCAAATATTGATAATGCG
AAAATTGAAGGCATTGAAATTGAAGCGTTGTTCGAGACTGGTGTGATTGAACATCGTGTTTCTGCAGAATGGAAAGATCC
TCGAGATAAGTCAGACGATAGCTTTTTGATCCGCAGAGCGCGCGAAAACTTCAGCTGGGTGTCGACCTATTCGGCTGATC
GCTTTGGATTTTCTGCCGTTGCGAATTACGTGGGTGATCGCAAAGACAGTACGGGCAAAACGATGGATGCCTATACTTTG
CTGGATATAAGTGCTAACTACAAGATGACAGAAGCATTAACGCTAGGTGCCAGAGTCGGTAACCTGTTTGACAAAGAGTA
TCAAACCGCACATAGCTCGGGTGGAAAATACTACGTAGGTGAAGGCCGAAATTGGTTTGCAACGGTCAATTACCGTTTCT
AA

Upstream 100 bases:

>100_bases
GGTTAACAGCTCTTGAGTCCGAATACCTGCCAGCAACTGAGCAAACACTGGAAGCCTTATCTTCATGCTCGGTAGGATTT
ACGCGATTTAGGATTACAAT

Downstream 100 bases:

>100_bases
TGTTTAAAAACCGCCTTCGGGCGGTTTATTTTTCAGAGTACGTGATGAAAAAGAAGGTTGTTATTAGTTGGTCAAGTGGC
AAAGATTCCACTCTGACCTT

Product: outer membrane cobalamin receptor protein

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator

Number of amino acids: Translated: 613; Mature: 613

Protein sequence:

>613_residues
MQKSLLAIAMASLLTPISYLHAQEVQTNDTVVVTANRFEQVESSVLASISVVTKAEIEQLNVTTALDILKTLPGVEVNSQ
GGKGQISSIFLRGTSSKHTLVLVDGVKINSATAGGASLGLIPAFAIEQIEVVRGPRAAIYGSDAIGGVIHIKTIPANRQT
KHDANLGYGNDDHSSLAWRSSGQLNDSTQASFVFSDEKSDGYRVNEVAPSGDSHGYQSQTLFGSLRHEIDDAWSVQFNGY
QLSSDVEYANQFSGVKNESNTDFYSIAGSLNFHKDNYSSQLMVSRSDNESWDGVASGMVAKTALFSSRNSVSWLNHWVVV
PALTLAAGVDYDQENARQGGANATNYSKTEKDNKAAYVTAHFSKNIVTAEASIRSDDDSAFGKHNTWNLGLGIAPSDYVE
FIASTGTGFKAPTFNDLYWPSSGNPNLKPETSKSSEVGIRSYLPFVQIDISAYRNEIEDMIDWAPTGPGGAWTPSNIDNA
KIEGIEIEALFETGVIEHRVSAEWKDPRDKSDDSFLIRRARENFSWVSTYSADRFGFSAVANYVGDRKDSTGKTMDAYTL
LDISANYKMTEALTLGARVGNLFDKEYQTAHSSGGKYYVGEGRNWFATVNYRF

Sequences:

>Translated_613_residues
MQKSLLAIAMASLLTPISYLHAQEVQTNDTVVVTANRFEQVESSVLASISVVTKAEIEQLNVTTALDILKTLPGVEVNSQ
GGKGQISSIFLRGTSSKHTLVLVDGVKINSATAGGASLGLIPAFAIEQIEVVRGPRAAIYGSDAIGGVIHIKTIPANRQT
KHDANLGYGNDDHSSLAWRSSGQLNDSTQASFVFSDEKSDGYRVNEVAPSGDSHGYQSQTLFGSLRHEIDDAWSVQFNGY
QLSSDVEYANQFSGVKNESNTDFYSIAGSLNFHKDNYSSQLMVSRSDNESWDGVASGMVAKTALFSSRNSVSWLNHWVVV
PALTLAAGVDYDQENARQGGANATNYSKTEKDNKAAYVTAHFSKNIVTAEASIRSDDDSAFGKHNTWNLGLGIAPSDYVE
FIASTGTGFKAPTFNDLYWPSSGNPNLKPETSKSSEVGIRSYLPFVQIDISAYRNEIEDMIDWAPTGPGGAWTPSNIDNA
KIEGIEIEALFETGVIEHRVSAEWKDPRDKSDDSFLIRRARENFSWVSTYSADRFGFSAVANYVGDRKDSTGKTMDAYTL
LDISANYKMTEALTLGARVGNLFDKEYQTAHSSGGKYYVGEGRNWFATVNYRF
>Mature_613_residues
MQKSLLAIAMASLLTPISYLHAQEVQTNDTVVVTANRFEQVESSVLASISVVTKAEIEQLNVTTALDILKTLPGVEVNSQ
GGKGQISSIFLRGTSSKHTLVLVDGVKINSATAGGASLGLIPAFAIEQIEVVRGPRAAIYGSDAIGGVIHIKTIPANRQT
KHDANLGYGNDDHSSLAWRSSGQLNDSTQASFVFSDEKSDGYRVNEVAPSGDSHGYQSQTLFGSLRHEIDDAWSVQFNGY
QLSSDVEYANQFSGVKNESNTDFYSIAGSLNFHKDNYSSQLMVSRSDNESWDGVASGMVAKTALFSSRNSVSWLNHWVVV
PALTLAAGVDYDQENARQGGANATNYSKTEKDNKAAYVTAHFSKNIVTAEASIRSDDDSAFGKHNTWNLGLGIAPSDYVE
FIASTGTGFKAPTFNDLYWPSSGNPNLKPETSKSSEVGIRSYLPFVQIDISAYRNEIEDMIDWAPTGPGGAWTPSNIDNA
KIEGIEIEALFETGVIEHRVSAEWKDPRDKSDDSFLIRRARENFSWVSTYSADRFGFSAVANYVGDRKDSTGKTMDAYTL
LDISANYKMTEALTLGARVGNLFDKEYQTAHSSGGKYYVGEGRNWFATVNYRF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily

Homologues:

Organism=Escherichia coli, GI1790405, Length=631, Percent_Identity=34.3898573692552, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI1788478, Length=129, Percent_Identity=44.1860465116279, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1786798, Length=275, Percent_Identity=29.4545454545455, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): BTUB_VIBVY (Q7MQ42)

Other databases:

- EMBL:   BA000037
- RefSeq:   NP_932959.1
- ProteinModelPortal:   Q7MQ42
- SMR:   Q7MQ42
- STRING:   Q7MQ42
- GeneID:   2622925
- GenomeReviews:   BA000037_GR
- KEGG:   vvy:VV0166
- NMPDR:   fig|196600.1.peg.235
- eggNOG:   COG4206
- HOGENOM:   HBG644936
- OMA:   NEYALAP
- ProtClustDB:   CLSK241564
- BioCyc:   VVUL196600:VV0166-MONOMER
- HAMAP:   MF_01531
- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010916
- Gene3D:   G3DSA:2.170.130.10
- Gene3D:   G3DSA:2.40.170.20

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec

EC number: NA

Molecular weight: Translated: 66778; Mature: 66778

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00430 TONB_DEPENDENT_REC_1; PS01156 TONB_DEPENDENT_REC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKSLLAIAMASLLTPISYLHAQEVQTNDTVVVTANRFEQVESSVLASISVVTKAEIEQL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
NVTTALDILKTLPGVEVNSQGGKGQISSIFLRGTSSKHTLVLVDGVKINSATAGGASLGL
HHHHHHHHHHHCCCCEEECCCCCCCEEEEEEECCCCCCEEEEEECEEEECCCCCCCCCCH
IPAFAIEQIEVVRGPRAAIYGSDAIGGVIHIKTIPANRQTKHDANLGYGNDDHSSLAWRS
HHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECC
SGQLNDSTQASFVFSDEKSDGYRVNEVAPSGDSHGYQSQTLFGSLRHEIDDAWSVQFNGY
CCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCE
QLSSDVEYANQFSGVKNESNTDFYSIAGSLNFHKDNYSSQLMVSRSDNESWDGVASGMVA
EECCCHHHHHHHCCCCCCCCCCEEEEECEEEECCCCCCCEEEEECCCCCCCCHHHHHHHH
KTALFSSRNSVSWLNHWVVVPALTLAAGVDYDQENARQGGANATNYSKTEKDNKAAYVTA
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEE
HFSKNIVTAEASIRSDDDSAFGKHNTWNLGLGIAPSDYVEFIASTGTGFKAPTFNDLYWP
ECCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCEECC
SSGNPNLKPETSKSSEVGIRSYLPFVQIDISAYRNEIEDMIDWAPTGPGGAWTPSNIDNA
CCCCCCCCCCCCCCCCCCHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
KIEGIEIEALFETGVIEHRVSAEWKDPRDKSDDSFLIRRARENFSWVSTYSADRFGFSAV
EECCEEEEEEECCCCCHHCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCHHHH
ANYVGDRKDSTGKTMDAYTLLDISANYKMTEALTLGARVGNLFDKEYQTAHSSGGKYYVG
HHHHCCCCCCCCCEEEEEEEEEECCCCEEEHHEEHHHHHHHHHHHHHHHHHCCCCEEEEC
EGRNWFATVNYRF
CCCCEEEEEEECC
>Mature Secondary Structure
MQKSLLAIAMASLLTPISYLHAQEVQTNDTVVVTANRFEQVESSVLASISVVTKAEIEQL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
NVTTALDILKTLPGVEVNSQGGKGQISSIFLRGTSSKHTLVLVDGVKINSATAGGASLGL
HHHHHHHHHHHCCCCEEECCCCCCCEEEEEEECCCCCCEEEEEECEEEECCCCCCCCCCH
IPAFAIEQIEVVRGPRAAIYGSDAIGGVIHIKTIPANRQTKHDANLGYGNDDHSSLAWRS
HHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECC
SGQLNDSTQASFVFSDEKSDGYRVNEVAPSGDSHGYQSQTLFGSLRHEIDDAWSVQFNGY
CCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCE
QLSSDVEYANQFSGVKNESNTDFYSIAGSLNFHKDNYSSQLMVSRSDNESWDGVASGMVA
EECCCHHHHHHHCCCCCCCCCCEEEEECEEEECCCCCCCEEEEECCCCCCCCHHHHHHHH
KTALFSSRNSVSWLNHWVVVPALTLAAGVDYDQENARQGGANATNYSKTEKDNKAAYVTA
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEE
HFSKNIVTAEASIRSDDDSAFGKHNTWNLGLGIAPSDYVEFIASTGTGFKAPTFNDLYWP
ECCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCEECC
SSGNPNLKPETSKSSEVGIRSYLPFVQIDISAYRNEIEDMIDWAPTGPGGAWTPSNIDNA
CCCCCCCCCCCCCCCCCCHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
KIEGIEIEALFETGVIEHRVSAEWKDPRDKSDDSFLIRRARENFSWVSTYSADRFGFSAV
EECCEEEEEEECCCCCHHCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCHHHH
ANYVGDRKDSTGKTMDAYTLLDISANYKMTEALTLGARVGNLFDKEYQTAHSSGGKYYVG
HHHHCCCCCCCCCEEEEEEEEEECCCCEEEHHEEHHHHHHHHHHHHHHHHHCCCCEEEEC
EGRNWFATVNYRF
CCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA