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The map label for this gene is gyrB

Identifier: 37678198

GI number: 37678198

Start: 11125

End: 13542

Strand: Direct

Name: gyrB

Synonym: VV0014

Alternate gene names: 37678198

Gene position: 11125-13542 (Clockwise)

Preceding gene: 37678197

Following gene: 37678199

Centisome position: 0.33

GC content: 49.21

Gene sequence:

>2418_bases
ATGTCGAACAATTACGATTCATCGAGTATTAAGGTACTGAAGGGTCTGGATGCGGTACGTAAGCGTCCGGGTATGTACAT
CGGGGACACAGATGATGGCACCGGTCTGCACCACATGGTTTTTGAGGTGGTGGATAACTCAATTGATGAAGCCTTAGCAG
GCCACTGTAAAGATATCGTAGTCACGATTCATGAGGATAACTCAGTTTCTGTTAGCGATGATGGCCGTGGTATCCCGACC
GAAATGCACCCAGAAGAGAACGTGTCTGCTGCCGAAGTTATCATGACGGTACTGCACGCTGGTGGTAAGTTTGACGACAA
CTCTTACAAAGTGTCTGGCGGTCTTCACGGTGTAGGTGTTTCGGTGGTAAACGCACTGTCTGAAAAAGTGCTGTTGACGA
TTCATCGTGGTGGTCATACCCACAGCCAAACCTATCGTCATGGTGTGCCTGATGCACCGCTTGCTATCATCGGCGATACC
GAAAAAACCGGTACCACGGTACGTTTCTGGCCAAGTGCTGAAACCTTCACCAACATCGAATTTCATTATGACATCCTAGC
GAAGCGTCTACGTGAGCTCTCTTTCCTGAACTCTGGCGTGTCGATCAAACTGTTTGATGAGCGCGAAGAAGATAAGAAAG
ATCACTTCATGTATGAAGGTGGTATTCAAGCGTTCGTCACTCACTTGAACCGCAACAAAACCCCCATCCATGAAAAAGTA
TTCCATTTTAATGCCGAGCGTGAAGACGGTATTGCGGTTGAAGTGGCGATGCAGTGGAACGATGGTTTCCAAGAAAACAT
CTACTGTTTTACCAACAACATCCCACAGCGTGACGGTGGTACCCACTTAGCGGGTTTCCGCGCGGCATTGACGCGCACAC
TGAACAGCTACATGGACAAAGAAGGCTACTCGAAGAAAGCGAAAACCGCGACTTCTGGTGACGATGCGCGTGAAGGTTTG
ACCGCTGTTGTTTCAGTTAAAGTGCCGGATCCAAAATTCTCAAGCCAAACCAAAGACAAACTGGTTTCTAGTGAAGTGAA
GTCCGCAGTGGAATCCTTCATGGCAGACAAACTGAACGACTTCTTGGCCGAACACCCAAGCGAAGCGAAAACCGTTTGTT
CTAAGATTATCGACGCCGCACGTGCGCGTGAAGCTGCACGTAAAGCGCGTGAAATGACACGCCGTAAAGGGGCGTTAGAC
CTTGCTGGCCTTCCTGGTAAACTTGCTGACTGTCAGGAAAAAGATCCTGCACTGTCTGAACTTTACATAGTGGAGGGTGA
TTCGGCAGGCGGCTCCGCAAAACAAGGCCGTAACCGTAAGAACCAAGCAATCCTACCGCTAAAAGGTAAGATTCTAAACG
TAGAAAAAGCGCGTTTCGACAAGATGCTCTCTTCTCAAGAAGTTGCAACGCTGATCACCGCACTAGGTTGTGGTATCGGT
CGTGACGAGTACAACCCAGACAAACTGCGTTACCACAACATCATCATCATGACCGATGCCGACGTCGATGGTTCGCACAT
CCGTACGCTACTGTTGACCTTCTTCTACCGTCAAATGCCGGAGCTTATTGAGCGTGGTTACGTGTATATCGCTCAGCCAC
CACTGTACAAAGTGAAGAAAGGCAAACAAGAGCAGTACATTAAAGATGAAGATGCGATGAACCAGTACCAAATTTCCTTG
GCATTGGATAACGCAGCGCTGCACGTGAACCCAGACGCACCAGCATTGGCTGGTGAAGCACTAGAGAAACTGGTTCAACA
ATACAATGCAGCGATTAAGCTGGTGGATCGCATGAGCCGTCGTTACCCACGTGCACTGATTCATGAACTGATTTATGTTC
CGCGTCTCAGCGAAACACAATTTGCCGATCAAGCAGCAATGGCAGCATGGACAGAGCAACTGGTTACTCAGTTGAACGCC
AAAGAAGTGGGCGCAAGCCAATACAGTTTTGAAATCGAACACGATGCAGAGCGCGGACTGTTCTCACCGAAGATTGTGGT
TCGTACCCACGGTGTGATGCACGAGTACCCACTGAGCGTGGATCTGATCAACTCGAAAGAATACCAGAAGCTGGCAGAGC
TGGCGGATGCGCTGGACGGTTTGATCGAAGAAGGTGCGTATATTCAGCGTGGTGAACGTACTCAACCCGTGAACAGTTTT
GCAAGTGCGCTTGAGTGGCTGATTAAGGAATCTCGTCGTGGTTTGAGCCTACAACGCTACAAAGGTTTGGGTGAGATGAA
CCCAGATCAGCTTTGGGAAACCACCATGGATCCAGAAACTCGCCGCATGATGCGCGTGACCATTGAAGACGCCGTTGGTG
CGGATGAGCTGTTCACGACCTTGATGGGTGATCAAGTTGAACCACGTCGTAACTTCATCGAAGAAAACGCATTGAAAGTA
GCGAACCTAGACGTGTAA

Upstream 100 bases:

>100_bases
TACGGAAAACCAGATCAGCGACATGCGCGATGACTCTGGCCGACTGTTTAATGTGGAGCAGGGTGTAATCGAGCAAGGAT
AAATAATGAGAGAGTAACTC

Downstream 100 bases:

>100_bases
TCGATAAGACGCGATTAAAATTAATGAAAATGCCACCTCGCAAGAGGTGGCATTTTTACTTAGGGCAGGAAAAATTGTCC
TAGACCCTAGACCCTAGGAA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 805; Mature: 804

Protein sequence:

>805_residues
MSNNYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCKDIVVTIHEDNSVSVSDDGRGIPT
EMHPEENVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEKVLLTIHRGGHTHSQTYRHGVPDAPLAIIGDT
EKTGTTVRFWPSAETFTNIEFHYDILAKRLRELSFLNSGVSIKLFDEREEDKKDHFMYEGGIQAFVTHLNRNKTPIHEKV
FHFNAEREDGIAVEVAMQWNDGFQENIYCFTNNIPQRDGGTHLAGFRAALTRTLNSYMDKEGYSKKAKTATSGDDAREGL
TAVVSVKVPDPKFSSQTKDKLVSSEVKSAVESFMADKLNDFLAEHPSEAKTVCSKIIDAARAREAARKAREMTRRKGALD
LAGLPGKLADCQEKDPALSELYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
RDEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYVYIAQPPLYKVKKGKQEQYIKDEDAMNQYQISL
ALDNAALHVNPDAPALAGEALEKLVQQYNAAIKLVDRMSRRYPRALIHELIYVPRLSETQFADQAAMAAWTEQLVTQLNA
KEVGASQYSFEIEHDAERGLFSPKIVVRTHGVMHEYPLSVDLINSKEYQKLAELADALDGLIEEGAYIQRGERTQPVNSF
ASALEWLIKESRRGLSLQRYKGLGEMNPDQLWETTMDPETRRMMRVTIEDAVGADELFTTLMGDQVEPRRNFIEENALKV
ANLDV

Sequences:

>Translated_805_residues
MSNNYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCKDIVVTIHEDNSVSVSDDGRGIPT
EMHPEENVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEKVLLTIHRGGHTHSQTYRHGVPDAPLAIIGDT
EKTGTTVRFWPSAETFTNIEFHYDILAKRLRELSFLNSGVSIKLFDEREEDKKDHFMYEGGIQAFVTHLNRNKTPIHEKV
FHFNAEREDGIAVEVAMQWNDGFQENIYCFTNNIPQRDGGTHLAGFRAALTRTLNSYMDKEGYSKKAKTATSGDDAREGL
TAVVSVKVPDPKFSSQTKDKLVSSEVKSAVESFMADKLNDFLAEHPSEAKTVCSKIIDAARAREAARKAREMTRRKGALD
LAGLPGKLADCQEKDPALSELYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
RDEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYVYIAQPPLYKVKKGKQEQYIKDEDAMNQYQISL
ALDNAALHVNPDAPALAGEALEKLVQQYNAAIKLVDRMSRRYPRALIHELIYVPRLSETQFADQAAMAAWTEQLVTQLNA
KEVGASQYSFEIEHDAERGLFSPKIVVRTHGVMHEYPLSVDLINSKEYQKLAELADALDGLIEEGAYIQRGERTQPVNSF
ASALEWLIKESRRGLSLQRYKGLGEMNPDQLWETTMDPETRRMMRVTIEDAVGADELFTTLMGDQVEPRRNFIEENALKV
ANLDV
>Mature_804_residues
SNNYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCKDIVVTIHEDNSVSVSDDGRGIPTE
MHPEENVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEKVLLTIHRGGHTHSQTYRHGVPDAPLAIIGDTE
KTGTTVRFWPSAETFTNIEFHYDILAKRLRELSFLNSGVSIKLFDEREEDKKDHFMYEGGIQAFVTHLNRNKTPIHEKVF
HFNAEREDGIAVEVAMQWNDGFQENIYCFTNNIPQRDGGTHLAGFRAALTRTLNSYMDKEGYSKKAKTATSGDDAREGLT
AVVSVKVPDPKFSSQTKDKLVSSEVKSAVESFMADKLNDFLAEHPSEAKTVCSKIIDAARAREAARKAREMTRRKGALDL
AGLPGKLADCQEKDPALSELYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGR
DEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYVYIAQPPLYKVKKGKQEQYIKDEDAMNQYQISLA
LDNAALHVNPDAPALAGEALEKLVQQYNAAIKLVDRMSRRYPRALIHELIYVPRLSETQFADQAAMAAWTEQLVTQLNAK
EVGASQYSFEIEHDAERGLFSPKIVVRTHGVMHEYPLSVDLINSKEYQKLAELADALDGLIEEGAYIQRGERTQPVNSFA
SALEWLIKESRRGLSLQRYKGLGEMNPDQLWETTMDPETRRMMRVTIEDAVGADELFTTLMGDQVEPRRNFIEENALKVA
NLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=549, Percent_Identity=25.3187613843352, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI19913406, Length=561, Percent_Identity=25.4901960784314, Blast_Score=128, Evalue=2e-29,
Organism=Escherichia coli, GI48994957, Length=806, Percent_Identity=77.6674937965261, Blast_Score=1293, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=556, Percent_Identity=39.3884892086331, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17535065, Length=542, Percent_Identity=26.0147601476015, Blast_Score=142, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI212645845, Length=524, Percent_Identity=24.0458015267176, Blast_Score=126, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI212645657, Length=153, Percent_Identity=33.9869281045752, Blast_Score=75, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=598, Percent_Identity=25.752508361204, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI17136538, Length=530, Percent_Identity=25.8490566037736, Blast_Score=131, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 89756; Mature: 89625

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCKDIV
CCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEE
VTIHEDNSVSVSDDGRGIPTEMHPEENVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGV
EEEECCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
SVVNALSEKVLLTIHRGGHTHSQTYRHGVPDAPLAIIGDTEKTGTTVRFWPSAETFTNIE
HHHHHHCCCEEEEEECCCCCCCHHHHCCCCCCCEEEEECCCCCCCEEEECCCCCCCEECE
FHYDILAKRLRELSFLNSGVSIKLFDEREEDKKDHFMYEGGIQAFVTHLNRNKTPIHEKV
EHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHEEECCCHHHHHHHHCCCCCCHHHHH
FHFNAEREDGIAVEVAMQWNDGFQENIYCFTNNIPQRDGGTHLAGFRAALTRTLNSYMDK
HCCCCCCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCC
EGYSKKAKTATSGDDAREGLTAVVSVKVPDPKFSSQTKDKLVSSEVKSAVESFMADKLND
CCCCCCCCCCCCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FLAEHPSEAKTVCSKIIDAARAREAARKAREMTRRKGALDLAGLPGKLADCQEKDPALSE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCEE
LYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
EEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCC
RDEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYVYIAQPPLYKVKK
CCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHC
GKQEQYIKDEDAMNQYQISLALDNAALHVNPDAPALAGEALEKLVQQYNAAIKLVDRMSR
CCHHHHCCCHHCCCEEEEEEEEECEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RYPRALIHELIYVPRLSETQFADQAAMAAWTEQLVTQLNAKEVGASQYSFEIEHDAERGL
HCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHCCCCCEEEEECCCHHCCC
FSPKIVVRTHGVMHEYPLSVDLINSKEYQKLAELADALDGLIEEGAYIQRGERTQPVNSF
CCCEEEEEECCCCEECCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHH
ASALEWLIKESRRGLSLQRYKGLGEMNPDQLWETTMDPETRRMMRVTIEDAVGADELFTT
HHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHCCCCHHHHHHEEEEHHHHCCHHHHHHH
LMGDQVEPRRNFIEENALKVANLDV
HHCCCCCHHHHHHHHCCEEEECCCC
>Mature Secondary Structure 
SNNYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCKDIV
CCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEE
VTIHEDNSVSVSDDGRGIPTEMHPEENVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGV
EEEECCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
SVVNALSEKVLLTIHRGGHTHSQTYRHGVPDAPLAIIGDTEKTGTTVRFWPSAETFTNIE
HHHHHHCCCEEEEEECCCCCCCHHHHCCCCCCCEEEEECCCCCCCEEEECCCCCCCEECE
FHYDILAKRLRELSFLNSGVSIKLFDEREEDKKDHFMYEGGIQAFVTHLNRNKTPIHEKV
EHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHEEECCCHHHHHHHHCCCCCCHHHHH
FHFNAEREDGIAVEVAMQWNDGFQENIYCFTNNIPQRDGGTHLAGFRAALTRTLNSYMDK
HCCCCCCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCC
EGYSKKAKTATSGDDAREGLTAVVSVKVPDPKFSSQTKDKLVSSEVKSAVESFMADKLND
CCCCCCCCCCCCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FLAEHPSEAKTVCSKIIDAARAREAARKAREMTRRKGALDLAGLPGKLADCQEKDPALSE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCEE
LYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
EEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCC
RDEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYVYIAQPPLYKVKK
CCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHC
GKQEQYIKDEDAMNQYQISLALDNAALHVNPDAPALAGEALEKLVQQYNAAIKLVDRMSR
CCHHHHCCCHHCCCEEEEEEEEECEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RYPRALIHELIYVPRLSETQFADQAAMAAWTEQLVTQLNAKEVGASQYSFEIEHDAERGL
HCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHCCCCCEEEEECCCHHCCC
FSPKIVVRTHGVMHEYPLSVDLINSKEYQKLAELADALDGLIEEGAYIQRGERTQPVNSF
CCCEEEEEECCCCEECCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHH
ASALEWLIKESRRGLSLQRYKGLGEMNPDQLWETTMDPETRRMMRVTIEDAVGADELFTT
HHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHCCCCHHHHHHEEEEHHHHCCHHHHHHH
LMGDQVEPRRNFIEENALKVANLDV
HHCCCCCHHHHHHHHCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]