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The map label for this gene is recF

Identifier: 37678197

GI number: 37678197

Start: 10027

End: 11106

Strand: Direct

Name: recF

Synonym: VV0013

Alternate gene names: 37678197

Gene position: 10027-11106 (Clockwise)

Preceding gene: 37678196

Following gene: 37678198

Centisome position: 0.3

GC content: 46.48

Gene sequence:

>1080_bases
ATGCCGCTTTCTCGTCTGATTATTCAGCAGTTTCGTAATATCAAAGCCTGCGACATTGCACTGTCGCCAGGCTTTAACTT
TCTTATTGGTCCGAATGGCAGTGGCAAAACCAGTGTCTTGGAAGCCATCTATTTGCTCGGTCATGGGCGCTCATTTAAAA
GTGCTTTGACCGGACGCGTGATTCAAAATGAGTGTGATCAACTGTTTGTCCATGGTCGTTTTTTGAACTCGGATCAATTT
GAGCTACCGATCGGCATTAATAAGCAGCGTGATGGCACAACAGAGGTTAAAATAGGCGGTCAATCTGGGCAAAAGTTGGC
GCAGCTTGCACAAGTGTTGCCATTGCAATTGATTCATCCCGAAGGCTTCGATTTATTAACGGATGGACCCAAGCATCGTC
GAGCGTTTATTGATTGGGGCGTGTTTCATACTGAGCCCGCGTTTTATGATGCATGGGGGCGTTTTAAGCGCCTCAACAAA
CAACGTAATGCGTTGTTGAAGTCGGCAAAGAGCTATCAAGAGCTGAGTTATTGGGATAAGGAAATGGCACGTTTGGCCGA
GTTGATTAGCCAATGGCGCGCCGATTACGTTGCCCAAATGCAAAGCAAAGCGGAACAGTTGTGCCAAGAATTTTTGCCAG
AATTTCATATCCAATTGAAGTATTATCGTGGCTGGGAAAAAGAAACCCCATACCAACAAATACTGGAAGAGAATTTTGAG
CGCGATCAAACGCTAGGCTATACCGTTAGCGGACCAAACAAGGCCGATTTACGGATAAAAGTGAACAACACCCCGGTGGA
AGATGTGTTATCGCGTGGCCAATTGAAATTAATGGTGTGTGCGCTCCGCTTGGCACAAGGCCAGCACCTAACGGAAAAAA
CCGGTAAGCAATGCGTCTATCTCATAGATGACTTTGCCTCTGAATTAGACAGTCAACGTCGCAAGCGTCTTGCTGATTGC
TTGAAACAGACGGGGGCTCAAGTTTTTGTAAGTTCGATTACGGAAAACCAGATCAGCGACATGCGCGATGACTCTGGCCG
ACTGTTTAATGTGGAGCAGGGTGTAATCGAGCAAGGATAA

Upstream 100 bases:

>100_bases
AATCTCAATGTCGGATGCCAACGCCAGTGCGCTGGTTGAAAATGTCGATGACGACAGCGCCATGTACGTGGTTATGCCAA
TTCGTCTATAAGCTGATTGA

Downstream 100 bases:

>100_bases
ATAATGAGAGAGTAACTCATGTCGAACAATTACGATTCATCGAGTATTAAGGTACTGAAGGGTCTGGATGCGGTACGTAA
GCGTCCGGGTATGTACATCG

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MPLSRLIIQQFRNIKACDIALSPGFNFLIGPNGSGKTSVLEAIYLLGHGRSFKSALTGRVIQNECDQLFVHGRFLNSDQF
ELPIGINKQRDGTTEVKIGGQSGQKLAQLAQVLPLQLIHPEGFDLLTDGPKHRRAFIDWGVFHTEPAFYDAWGRFKRLNK
QRNALLKSAKSYQELSYWDKEMARLAELISQWRADYVAQMQSKAEQLCQEFLPEFHIQLKYYRGWEKETPYQQILEENFE
RDQTLGYTVSGPNKADLRIKVNNTPVEDVLSRGQLKLMVCALRLAQGQHLTEKTGKQCVYLIDDFASELDSQRRKRLADC
LKQTGAQVFVSSITENQISDMRDDSGRLFNVEQGVIEQG

Sequences:

>Translated_359_residues
MPLSRLIIQQFRNIKACDIALSPGFNFLIGPNGSGKTSVLEAIYLLGHGRSFKSALTGRVIQNECDQLFVHGRFLNSDQF
ELPIGINKQRDGTTEVKIGGQSGQKLAQLAQVLPLQLIHPEGFDLLTDGPKHRRAFIDWGVFHTEPAFYDAWGRFKRLNK
QRNALLKSAKSYQELSYWDKEMARLAELISQWRADYVAQMQSKAEQLCQEFLPEFHIQLKYYRGWEKETPYQQILEENFE
RDQTLGYTVSGPNKADLRIKVNNTPVEDVLSRGQLKLMVCALRLAQGQHLTEKTGKQCVYLIDDFASELDSQRRKRLADC
LKQTGAQVFVSSITENQISDMRDDSGRLFNVEQGVIEQG
>Mature_358_residues
PLSRLIIQQFRNIKACDIALSPGFNFLIGPNGSGKTSVLEAIYLLGHGRSFKSALTGRVIQNECDQLFVHGRFLNSDQFE
LPIGINKQRDGTTEVKIGGQSGQKLAQLAQVLPLQLIHPEGFDLLTDGPKHRRAFIDWGVFHTEPAFYDAWGRFKRLNKQ
RNALLKSAKSYQELSYWDKEMARLAELISQWRADYVAQMQSKAEQLCQEFLPEFHIQLKYYRGWEKETPYQQILEENFER
DQTLGYTVSGPNKADLRIKVNNTPVEDVLSRGQLKLMVCALRLAQGQHLTEKTGKQCVYLIDDFASELDSQRRKRLADCL
KQTGAQVFVSSITENQISDMRDDSGRLFNVEQGVIEQG

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=356, Percent_Identity=56.7415730337079, Blast_Score=434, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 40948; Mature: 40817

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSRLIIQQFRNIKACDIALSPGFNFLIGPNGSGKTSVLEAIYLLGHGRSFKSALTGRV
CCHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
IQNECDQLFVHGRFLNSDQFELPIGINKQRDGTTEVKIGGQSGQKLAQLAQVLPLQLIHP
HHHHHHHHHHHCEEECCCCEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCEEECC
EGFDLLTDGPKHRRAFIDWGVFHTEPAFYDAWGRFKRLNKQRNALLKSAKSYQELSYWDK
CCCCCCCCCHHHHHHEEECCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMARLAELISQWRADYVAQMQSKAEQLCQEFLPEFHIQLKYYRGWEKETPYQQILEENFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEEEECCCCCCCCHHHHHHHHHC
RDQTLGYTVSGPNKADLRIKVNNTPVEDVLSRGQLKLMVCALRLAQGQHLTEKTGKQCVY
CCCCCCEEECCCCCCEEEEEECCCCHHHHHCCCCHHHHHHHHHHHCCCCHHHHCCCCEEH
LIDDFASELDSQRRKRLADCLKQTGAQVFVSSITENQISDMRDDSGRLFNVEQGVIEQG
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEECCHHHCCCC
>Mature Secondary Structure 
PLSRLIIQQFRNIKACDIALSPGFNFLIGPNGSGKTSVLEAIYLLGHGRSFKSALTGRV
CHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
IQNECDQLFVHGRFLNSDQFELPIGINKQRDGTTEVKIGGQSGQKLAQLAQVLPLQLIHP
HHHHHHHHHHHCEEECCCCEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCEEECC
EGFDLLTDGPKHRRAFIDWGVFHTEPAFYDAWGRFKRLNKQRNALLKSAKSYQELSYWDK
CCCCCCCCCHHHHHHEEECCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMARLAELISQWRADYVAQMQSKAEQLCQEFLPEFHIQLKYYRGWEKETPYQQILEENFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEEEECCCCCCCCHHHHHHHHHC
RDQTLGYTVSGPNKADLRIKVNNTPVEDVLSRGQLKLMVCALRLAQGQHLTEKTGKQCVY
CCCCCCEEECCCCCCEEEEEECCCCHHHHHCCCCHHHHHHHHHHHCCCCHHHHCCCCEEH
LIDDFASELDSQRRKRLADCLKQTGAQVFVSSITENQISDMRDDSGRLFNVEQGVIEQG
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEECCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA