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The map label for this gene is dnaN [H]

Identifier: 37678196

GI number: 37678196

Start: 8917

End: 10017

Strand: Direct

Name: dnaN [H]

Synonym: VV0012

Alternate gene names: 37678196

Gene position: 8917-10017 (Clockwise)

Preceding gene: 37678195

Following gene: 37678197

Centisome position: 0.27

GC content: 50.68

Gene sequence:

>1101_bases
ATGAAATTTACTATCGCACGTAGTCATTTTATTAAACCCCTTCAACAGGTTTCCGGCGCACTCGGTGGGCGACCAACGCT
ACCGATCCTCGGTAATTTGCTGATCAACGTTGAAAATGGTCAACTGTCAATGACCGCCACGGACTTAGAAGTGGAGTTGA
TCAGTAAAGTGACCTTGGAAGGGGAGTTTGAAGCGGGCAGCATTACGGTTCCTTCGCGCAAGTTTCTCGATATCTGCCGT
GGATTACCCGACGATGCGGTGATCACGTTTGTATTGGAAGGCGATCGCGTGCAAGTGCGCTCCGGGCGCAGCCGTTTCTC
GTTGGCCACTTTGCCAGCCAATGATTTTCCGAATATTGAAGATTGGCAAAGTGAAGTGGAAGTCTCCCTCACTCAGGGCG
AATTGCGCGCGCTGATTGAGAAAACACAATTCTCGATGGCGAACCAAGACGTACGCTACTACCTCAACGGTATGCTGTTT
GAGATTGATGGCAGCACACTGCGCAGTGTCGCGACCGATGGTCACCGTATGGCTGTGGCGCAGGCACCATTGAGTGCTGA
CTTCGCACAAAAGCAGATCATCGTACCGCGTAAAGGTGTGCAAGAGCTGGTTAAACTGCTGGATACACCAGAGCAACCCG
TCACGCTGCAGATTGGTAGCTCAAATCTGCGCGCTGAAGTAAACCATTTTGTCTTTACTTCTAAGCTGGTGGATGGTCGT
TTCCCGGATTACCGTCGCGTGTTGCCGCAACACACAACCAAAACGCTGGAAGCGAGTTGTGATGAATTACGCCAAGCGTT
TTCACGCGCGGCGATTTTGTCGAATGAAAAATTCCGTGGCGTTCGCGTTAACCTCGCTGGCAGTGAAATGCGCATTACCG
CCAACAACCCAGAGCAAGAAGAAGCGGAAGAGATGTTGGACGTGAGCTTCGACGGTGAGGCGATTGAGATTGGTTTCAAC
GTTAGCTACGTATTGGATGTGCTAAACACCTTGCGTTGTGAACAAGTACGAATCTCAATGTCGGATGCCAACGCCAGTGC
GCTGGTTGAAAATGTCGATGACGACAGCGCCATGTACGTGGTTATGCCAATTCGTCTATAA

Upstream 100 bases:

>100_bases
AGAAGATTACTCTAACTTGATTCGCACCCTGTCGTCTTAATCGGTTAGAATGACACAATTCTAAACACTTTATTGAAGCG
GACAGCGTAAAGAGCCAAGC

Downstream 100 bases:

>100_bases
GCTGATTGAATGCCGCTTTCTCGTCTGATTATTCAGCAGTTTCGTAATATCAAAGCCTGCGACATTGCACTGTCGCCAGG
CTTTAACTTTCTTATTGGTC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKFTIARSHFIKPLQQVSGALGGRPTLPILGNLLINVENGQLSMTATDLEVELISKVTLEGEFEAGSITVPSRKFLDICR
GLPDDAVITFVLEGDRVQVRSGRSRFSLATLPANDFPNIEDWQSEVEVSLTQGELRALIEKTQFSMANQDVRYYLNGMLF
EIDGSTLRSVATDGHRMAVAQAPLSADFAQKQIIVPRKGVQELVKLLDTPEQPVTLQIGSSNLRAEVNHFVFTSKLVDGR
FPDYRRVLPQHTTKTLEASCDELRQAFSRAAILSNEKFRGVRVNLAGSEMRITANNPEQEEAEEMLDVSFDGEAIEIGFN
VSYVLDVLNTLRCEQVRISMSDANASALVENVDDDSAMYVVMPIRL

Sequences:

>Translated_366_residues
MKFTIARSHFIKPLQQVSGALGGRPTLPILGNLLINVENGQLSMTATDLEVELISKVTLEGEFEAGSITVPSRKFLDICR
GLPDDAVITFVLEGDRVQVRSGRSRFSLATLPANDFPNIEDWQSEVEVSLTQGELRALIEKTQFSMANQDVRYYLNGMLF
EIDGSTLRSVATDGHRMAVAQAPLSADFAQKQIIVPRKGVQELVKLLDTPEQPVTLQIGSSNLRAEVNHFVFTSKLVDGR
FPDYRRVLPQHTTKTLEASCDELRQAFSRAAILSNEKFRGVRVNLAGSEMRITANNPEQEEAEEMLDVSFDGEAIEIGFN
VSYVLDVLNTLRCEQVRISMSDANASALVENVDDDSAMYVVMPIRL
>Mature_366_residues
MKFTIARSHFIKPLQQVSGALGGRPTLPILGNLLINVENGQLSMTATDLEVELISKVTLEGEFEAGSITVPSRKFLDICR
GLPDDAVITFVLEGDRVQVRSGRSRFSLATLPANDFPNIEDWQSEVEVSLTQGELRALIEKTQFSMANQDVRYYLNGMLF
EIDGSTLRSVATDGHRMAVAQAPLSADFAQKQIIVPRKGVQELVKLLDTPEQPVTLQIGSSNLRAEVNHFVFTSKLVDGR
FPDYRRVLPQHTTKTLEASCDELRQAFSRAAILSNEKFRGVRVNLAGSEMRITANNPEQEEAEEMLDVSFDGEAIEIGFN
VSYVLDVLNTLRCEQVRISMSDANASALVENVDDDSAMYVVMPIRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=366, Percent_Identity=63.9344262295082, Blast_Score=501, Evalue=1e-143,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40583; Mature: 40583

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTIARSHFIKPLQQVSGALGGRPTLPILGNLLINVENGQLSMTATDLEVELISKVTLE
CEEEECHHHHHHHHHHHHHHCCCCCCHHEECEEEEEEECCEEEEEEECCEEEEEHEEEEC
GEFEAGSITVPSRKFLDICRGLPDDAVITFVLEGDRVQVRSGRSRFSLATLPANDFPNIE
CCCCCCCEECCCHHHHHHHCCCCCCCEEEEEEECCEEEEECCCCEEEEEECCCCCCCCHH
DWQSEVEVSLTQGELRALIEKTQFSMANQDVRYYLNGMLFEIDGSTLRSVATDGHRMAVA
HHHHHEEEEECHHHHHHHHHHHHHHHCCCHHHEEECCEEEEECCHHHHHHHCCCCEEEEE
QAPLSADFAQKQIIVPRKGVQELVKLLDTPEQPVTLQIGSSNLRAEVNHFVFTSKLVDGR
ECCCCCCHHHCEEECCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHEEEEHHHCCC
FPDYRRVLPQHTTKTLEASCDELRQAFSRAAILSNEKFRGVRVNLAGSEMRITANNPEQE
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCHH
EAEEMLDVSFDGEAIEIGFNVSYVLDVLNTLRCEQVRISMSDANASALVENVDDDSAMYV
HHHHHHCCCCCCCEEEEECCHHHHHHHHHHHCEEEEEEEECCCCHHHHHCCCCCCCEEEE
VMPIRL
EEEEEC
>Mature Secondary Structure
MKFTIARSHFIKPLQQVSGALGGRPTLPILGNLLINVENGQLSMTATDLEVELISKVTLE
CEEEECHHHHHHHHHHHHHHCCCCCCHHEECEEEEEEECCEEEEEEECCEEEEEHEEEEC
GEFEAGSITVPSRKFLDICRGLPDDAVITFVLEGDRVQVRSGRSRFSLATLPANDFPNIE
CCCCCCCEECCCHHHHHHHCCCCCCCEEEEEEECCEEEEECCCCEEEEEECCCCCCCCHH
DWQSEVEVSLTQGELRALIEKTQFSMANQDVRYYLNGMLFEIDGSTLRSVATDGHRMAVA
HHHHHEEEEECHHHHHHHHHHHHHHHCCCHHHEEECCEEEEECCHHHHHHHCCCCEEEEE
QAPLSADFAQKQIIVPRKGVQELVKLLDTPEQPVTLQIGSSNLRAEVNHFVFTSKLVDGR
ECCCCCCHHHCEEECCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHEEEEHHHCCC
FPDYRRVLPQHTTKTLEASCDELRQAFSRAAILSNEKFRGVRVNLAGSEMRITANNPEQE
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCHH
EAEEMLDVSFDGEAIEIGFNVSYVLDVLNTLRCEQVRISMSDANASALVENVDDDSAMYV
HHHHHHCCCCCCCEEEEECCHHHHHHHHHHHCEEEEEEEECCCCHHHHHCCCCCCCEEEE
VMPIRL
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]