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The map label for this gene is cysB

Identifier: 161486632

GI number: 161486632

Start: 1343776

End: 1344750

Strand: Direct

Name: cysB

Synonym: VV1318

Alternate gene names: 161486632

Gene position: 1343776-1344750 (Clockwise)

Preceding gene: 37679498

Following gene: 37679503

Centisome position: 40.06

GC content: 44.1

Gene sequence:

>975_bases
ATGAAACTGCAACAACTGAAGTACATTGTTGAAGTCGTTAATCACAATCTAAATGTCTCGGCGACCGCCGAAAGCTTGTA
TACCTCTCAGCCAGGGATCAGTAAGCAGGTCAGGCTTTTAGAAGATGAGTTAGGCATTCAGATATTTGAACGAAGTGGTA
AACACCTCACTCAAGTGACTCGAGCTGGGGAAGATATCGTGCGAATTTCGCAAGAAATTTTAGCTCGAGTTGAGAGTATT
AAAGCTGTGGCAGGTGAGCATACTCATCCTGAAATGGGCACACTGAACATTTCTACCACCCATACACAGGCTCGTTATGC
TTTACCGGATGTCATTAAAGGATTTGTTAAGCGTTACCCTAAGGTGTCACTGCATATGCACCAAGGGACGCCAAGTCAAA
TGTCCGAAGCGATTGCAAAAGGCACGGCGAACTTTGCTATTGCGACAGAAGCTCTTCATTTGTACCAAGACGCAATTATG
TTGCCTTGTTACCATTGGAATCGTTCGATTGTTGTACCTAAAGAGCATCCATTGGCTAAGAAGGAAAAAGTGACTATTCA
AGATTTGGCTTCTTATCCATTAGTGACTTATGTGTTTGGTTTTACAGGTCGATCTGAATTAGACACCGCGTTCAATCGAG
TCGGATTAACACCGCGAGTGGTCTTTACGGCAACCGACGCGGATGTTATCAAAACCTATGTACGTATGGGGATTGGTGTG
GGTGTGATTGCCAGCATGGCGGTAGATAAAGAGCAGGACAAAGATCTGGTATCGATCGATGCGAGCCACTTGTTTGGTGC
AAGTACCACCAGTATCGGCTTCCGCCGAGGTACGTTCCTACGCTCATATATGTTTGATTTTATGGAAAGATTTGCACCTC
ATTTGACAAGACCAGTAGTAGAACAGGCGATCTCGTTGAAATCAAATGCTGAGATTGATGAAATGTTTAAAGACATCGTT
CTTCCCGTTCGTTAA

Upstream 100 bases:

>100_bases
GTTACACGTTATAACTAATAATTATATTTGAGAACTTAGTATGCGCAAGTTCTGCTCAAATGAGTGAGATTTGTTATTAG
TAACGGATTACGGGGCCAGT

Downstream 100 bases:

>100_bases
AGCGCTTTCCTCCACCGCTTCTTCCCCCTAAAATGGCGTCGCAATTTTAGGGGGATACATGCAAAAACAATTTCAATTGC
TCGATGAATGGCTTACTGAG

Product: transcriptional regulator CysB

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKLQQLKYIVEVVNHNLNVSATAESLYTSQPGISKQVRLLEDELGIQIFERSGKHLTQVTRAGEDIVRISQEILARVESI
KAVAGEHTHPEMGTLNISTTHTQARYALPDVIKGFVKRYPKVSLHMHQGTPSQMSEAIAKGTANFAIATEALHLYQDAIM
LPCYHWNRSIVVPKEHPLAKKEKVTIQDLASYPLVTYVFGFTGRSELDTAFNRVGLTPRVVFTATDADVIKTYVRMGIGV
GVIASMAVDKEQDKDLVSIDASHLFGASTTSIGFRRGTFLRSYMFDFMERFAPHLTRPVVEQAISLKSNAEIDEMFKDIV
LPVR

Sequences:

>Translated_324_residues
MKLQQLKYIVEVVNHNLNVSATAESLYTSQPGISKQVRLLEDELGIQIFERSGKHLTQVTRAGEDIVRISQEILARVESI
KAVAGEHTHPEMGTLNISTTHTQARYALPDVIKGFVKRYPKVSLHMHQGTPSQMSEAIAKGTANFAIATEALHLYQDAIM
LPCYHWNRSIVVPKEHPLAKKEKVTIQDLASYPLVTYVFGFTGRSELDTAFNRVGLTPRVVFTATDADVIKTYVRMGIGV
GVIASMAVDKEQDKDLVSIDASHLFGASTTSIGFRRGTFLRSYMFDFMERFAPHLTRPVVEQAISLKSNAEIDEMFKDIV
LPVR
>Mature_324_residues
MKLQQLKYIVEVVNHNLNVSATAESLYTSQPGISKQVRLLEDELGIQIFERSGKHLTQVTRAGEDIVRISQEILARVESI
KAVAGEHTHPEMGTLNISTTHTQARYALPDVIKGFVKRYPKVSLHMHQGTPSQMSEAIAKGTANFAIATEALHLYQDAIM
LPCYHWNRSIVVPKEHPLAKKEKVTIQDLASYPLVTYVFGFTGRSELDTAFNRVGLTPRVVFTATDADVIKTYVRMGIGV
GVIASMAVDKEQDKDLVSIDASHLFGASTTSIGFRRGTFLRSYMFDFMERFAPHLTRPVVEQAISLKSNAEIDEMFKDIV
LPVR

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=322, Percent_Identity=74.8447204968944, Blast_Score=526, Evalue=1e-151,
Organism=Escherichia coli, GI1788296, Length=313, Percent_Identity=39.6166134185303, Blast_Score=257, Evalue=6e-70,
Organism=Escherichia coli, GI1790399, Length=286, Percent_Identity=26.5734265734266, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI157672245, Length=235, Percent_Identity=26.3829787234043, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788887, Length=246, Percent_Identity=28.0487804878049, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787879, Length=250, Percent_Identity=26, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI2367136, Length=251, Percent_Identity=23.5059760956175, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787806, Length=287, Percent_Identity=23.6933797909408, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789204, Length=232, Percent_Identity=24.5689655172414, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI145693105, Length=186, Percent_Identity=24.7311827956989, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790262, Length=249, Percent_Identity=24.0963855421687, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787601, Length=153, Percent_Identity=29.4117647058824, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36161; Mature: 36161

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQQLKYIVEVVNHNLNVSATAESLYTSQPGISKQVRLLEDELGIQIFERSGKHLTQVT
CCHHHHHHHHHHHCCCCCEEEHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHCCHHHHHHH
RAGEDIVRISQEILARVESIKAVAGEHTHPEMGTLNISTTHTQARYALPDVIKGFVKRYP
HCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCC
KVSLHMHQGTPSQMSEAIAKGTANFAIATEALHLYQDAIMLPCYHWNRSIVVPKEHPLAK
CEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEECCCEEECCCCCCCCH
KEKVTIQDLASYPLVTYVFGFTGRSELDTAFNRVGLTPRVVFTATDADVIKTYVRMGIGV
HHCCHHHHHHCCCHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCH
GVIASMAVDKEQDKDLVSIDASHLFGASTTSIGFRRGTFLRSYMFDFMERFAPHLTRPVV
HHHHHHHHCCCCCCCEEEECHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQAISLKSNAEIDEMFKDIVLPVR
HHHHHCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MKLQQLKYIVEVVNHNLNVSATAESLYTSQPGISKQVRLLEDELGIQIFERSGKHLTQVT
CCHHHHHHHHHHHCCCCCEEEHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHCCHHHHHHH
RAGEDIVRISQEILARVESIKAVAGEHTHPEMGTLNISTTHTQARYALPDVIKGFVKRYP
HCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCC
KVSLHMHQGTPSQMSEAIAKGTANFAIATEALHLYQDAIMLPCYHWNRSIVVPKEHPLAK
CEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEECCCEEECCCCCCCCH
KEKVTIQDLASYPLVTYVFGFTGRSELDTAFNRVGLTPRVVFTATDADVIKTYVRMGIGV
HHCCHHHHHHCCCHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCH
GVIASMAVDKEQDKDLVSIDASHLFGASTTSIGFRRGTFLRSYMFDFMERFAPHLTRPVV
HHHHHHHHCCCCCCCEEEECHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQAISLKSNAEIDEMFKDIVLPVR
HHHHHCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]