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The map label for this gene is bioB

Identifier: 37523897

GI number: 37523897

Start: 4562756

End: 4563712

Strand: Direct

Name: bioB

Synonym: gvip564

Alternate gene names: 37523897

Gene position: 4562756-4563712 (Clockwise)

Preceding gene: 37523896

Following gene: 37523899

Centisome position: 97.93

GC content: 62.17

Gene sequence:

>957_bases
ATGTGCCGTGCGCCGATGCCCTCGACCGTCTCCGAGATTTCGCGGATCTATCACCAGCCGCTGCCCGATTTGCTCTTCGA
AGCCCAGCGCGTCCACCGTGCCCACCATGATCCGCGCGAAGTGCAGCTTTGTACCCTCAGCAACATCAAAACCGGTCTGT
GCCCCGAAAATTGTGGCTACTGCTCCCAGAGCGTTCACCACAAAAGCGGCCTGGCAGCCCAGGAACTCAGTTCCCTCGAC
GCGGTAATGGCCGAGGCGCAGGCAGCCAAGGCGGCGGGTTCGACTCGATTTTGCATGGGGGCTGCCTGGCGAGAAATCAA
AGACGGTCCGCCGTTCGAGCGGGTGTTGGAGATGGTGCGCTCGGTGGCGGCACTGGACATGGAAGTCTGCTGCACCCTCG
GTATGGTCAAACCGCATCAGGCCGAACGCCTGAAGCAGGCAGGACTCACCGCCTACAACCACAATCTCGACACCGGCCCC
GGCTACTACCCGCAGGTGGTGACCACCCGCACCTACAAGGACCGGCTCGAAACGATCCGGGCGGTGGGTGCGGCGGGTAT
TTCTGTGTGCTGCGGCGGCATCCTGGGCATGGGTGAATCGCTCACAGACCGCTTCGAGCTACTCGAATCGCTCGGCAGCC
TCGACCCAACTCCCGAGTCGATCCCGATCAACTGCCTAGTACCGGTGGCGGGCACCCCTCTGGCCGACTCGTCGCCCGTC
GAACCGCTCGACCTGGTGCGGATGGTTGCCACGACCCGTATTCTTTTTCCAGATGCCATGGTGCGCCTTTCGGCAGGGCG
CCTGCAGATGAGCGAGGAACTGCAGGCCCTTTGCTTCCTGGCCGGGGCCAATTCGATATTTACCGGCCCGAAACTGCTCA
CCACCCCCAACCCCGAGCACTCCCACGATCAGAAGCTGCTTGAAAAATTGGGTATGGAGCCAAAGACGACCCAGTGA

Upstream 100 bases:

>100_bases
TGAGCATTTTTCACGCGGCCCGCAACCACCCAATCCTGGGCGGCGGCGGGTCGTTTGCTTTGGATAGGGACGTGCGATCG
GGTAGGCTGTTGCTGCTCCG

Downstream 100 bases:

>100_bases
ACATCAATATTTTGCTTTGTGATTGCGCTAGGAAAACCTATTAGTTCTTCCAGCTTTTTTCCCTTGAGTTGCTTGTTTGA
GAACTGCGTCCAACGAATGC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MCRAPMPSTVSEISRIYHQPLPDLLFEAQRVHRAHHDPREVQLCTLSNIKTGLCPENCGYCSQSVHHKSGLAAQELSSLD
AVMAEAQAAKAAGSTRFCMGAAWREIKDGPPFERVLEMVRSVAALDMEVCCTLGMVKPHQAERLKQAGLTAYNHNLDTGP
GYYPQVVTTRTYKDRLETIRAVGAAGISVCCGGILGMGESLTDRFELLESLGSLDPTPESIPINCLVPVAGTPLADSSPV
EPLDLVRMVATTRILFPDAMVRLSAGRLQMSEELQALCFLAGANSIFTGPKLLTTPNPEHSHDQKLLEKLGMEPKTTQ

Sequences:

>Translated_318_residues
MCRAPMPSTVSEISRIYHQPLPDLLFEAQRVHRAHHDPREVQLCTLSNIKTGLCPENCGYCSQSVHHKSGLAAQELSSLD
AVMAEAQAAKAAGSTRFCMGAAWREIKDGPPFERVLEMVRSVAALDMEVCCTLGMVKPHQAERLKQAGLTAYNHNLDTGP
GYYPQVVTTRTYKDRLETIRAVGAAGISVCCGGILGMGESLTDRFELLESLGSLDPTPESIPINCLVPVAGTPLADSSPV
EPLDLVRMVATTRILFPDAMVRLSAGRLQMSEELQALCFLAGANSIFTGPKLLTTPNPEHSHDQKLLEKLGMEPKTTQ
>Mature_318_residues
MCRAPMPSTVSEISRIYHQPLPDLLFEAQRVHRAHHDPREVQLCTLSNIKTGLCPENCGYCSQSVHHKSGLAAQELSSLD
AVMAEAQAAKAAGSTRFCMGAAWREIKDGPPFERVLEMVRSVAALDMEVCCTLGMVKPHQAERLKQAGLTAYNHNLDTGP
GYYPQVVTTRTYKDRLETIRAVGAAGISVCCGGILGMGESLTDRFELLESLGSLDPTPESIPINCLVPVAGTPLADSSPV
EPLDLVRMVATTRILFPDAMVRLSAGRLQMSEELQALCFLAGANSIFTGPKLLTTPNPEHSHDQKLLEKLGMEPKTTQ

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=311, Percent_Identity=54.6623794212219, Blast_Score=355, Evalue=2e-99,
Organism=Saccharomyces cerevisiae, GI6321725, Length=313, Percent_Identity=49.2012779552716, Blast_Score=320, Evalue=2e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_GLOVI (Q7NDA8)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_927274.1
- HSSP:   P12996
- ProteinModelPortal:   Q7NDA8
- SMR:   Q7NDA8
- GeneID:   2602731
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.4328
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   CLSK2306281
- BioCyc:   GVIO251221:GLR4328-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 34494; Mature: 34494

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCRAPMPSTVSEISRIYHQPLPDLLFEAQRVHRAHHDPREVQLCTLSNIKTGLCPENCGY
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCH
CSQSVHHKSGLAAQELSSLDAVMAEAQAAKAAGSTRFCMGAAWREIKDGPPFERVLEMVR
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
SVAALDMEVCCTLGMVKPHQAERLKQAGLTAYNHNLDTGPGYYPQVVTTRTYKDRLETIR
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHH
AVGAAGISVCCGGILGMGESLTDRFELLESLGSLDPTPESIPINCLVPVAGTPLADSSPV
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCC
EPLDLVRMVATTRILFPDAMVRLSAGRLQMSEELQALCFLAGANSIFTGPKLLTTPNPEH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCC
SHDQKLLEKLGMEPKTTQ
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MCRAPMPSTVSEISRIYHQPLPDLLFEAQRVHRAHHDPREVQLCTLSNIKTGLCPENCGY
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCH
CSQSVHHKSGLAAQELSSLDAVMAEAQAAKAAGSTRFCMGAAWREIKDGPPFERVLEMVR
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
SVAALDMEVCCTLGMVKPHQAERLKQAGLTAYNHNLDTGPGYYPQVVTTRTYKDRLETIR
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHH
AVGAAGISVCCGGILGMGESLTDRFELLESLGSLDPTPESIPINCLVPVAGTPLADSSPV
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCC
EPLDLVRMVATTRILFPDAMVRLSAGRLQMSEELQALCFLAGANSIFTGPKLLTTPNPEH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCC
SHDQKLLEKLGMEPKTTQ
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292